Click here to switch to the map view.

The map label for this gene is prpC [H]

Identifier: 93006138

GI number: 93006138

Start: 1600112

End: 1601239

Strand: Reverse

Name: prpC [H]

Synonym: Pcryo_1310

Alternate gene names: 93006138

Gene position: 1601239-1600112 (Counterclockwise)

Preceding gene: 93006139

Following gene: 93006137

Centisome position: 52.33

GC content: 46.63

Gene sequence:

>1128_bases
ATGGCAGCACAAAAAGAACTTTCGGGCGCAGGTTTACGTGGTCAAGTAGCAGGTAAGACCGCTTTGTCTACCGTTGGCAA
ATCAGGCTCAGGTCTGACTTATCGCGGTTATGACGTATCAGAGCTGGCTGACAAGTGCATCTTTGAGGAAGTGGCTTATA
TGTTGCTATACGGCAACTTACCTACCCAAAGTGAGCTTGATGCTTATCAAACTAAATTGAAAGGTTTACGTGGCTTACCA
CAAGCATTAAAAGATGTGCTTGAGCGTATTCCTGCGGACGCCCATCCGATGGACGTATTACGTACCGGTTGCTCGATGCT
TGGTAACCTTGAGATGGAAACTGACTTCTCACAAGAGAACGATAAAACCGACCGTATGCTGGCGGTTTTCCCATCAATCA
TCAATTACTGGTATCGCTTTACCCACGACAACGTGCGTATCGAAACTGAAACTGATGACGATACCATTGGCGGTCATTTC
TTACATCTGCTAAAAGGTGAAAAACCAAACGAGCTACATACTAAGGTAATGAACGTATCACTGATTCTTTATGCTGAGCA
TGAGTTTAACGCCTCAACCTTTACCGCTCGCGTCTGTGCGTCTACCCTGTCTGACATTCATTCTTGTATCACTGGCGCGA
TTGGCTCATTACGCGGTCATTTACATGGCGGCGCCAATGAAGCGGCGATGGATATGATTGAAGGATTCACCTCTCCTGAT
CAAGCAGAGTCTGAGATGATGGCAATGCTGGCGCGTAAAGATAAGATTATGGGCTTTGGTCATGCTATCTATAGCGAATC
AGATCCACGTAATGTTGTAATTAAAGGTTGGGCTGAAAAACTGGCAGCAGATGTTGGTGATGAAGTGCTATACCCAGTAT
CAGTACGCTGTGAAGAAGTGATGTGGCGCGAGAAAAAACTCTTCTGTAATGCTGACTTTTTCCATGCGTCTGCCTATCAC
TTTATGGGCATTCCAACCAAACTGTTTACGCCAATCTTTGTTTGCTCGCGTCTAACAGGCTGGGCAGCACACGTGTTTGA
GCAACGTGCGAACAACCGTATCATTCGCCCAAGTGCGGAATATACTGGCGAAGAGCTGCGCCCAGTACCAGAGATGAGCG
CGCGTTAA

Upstream 100 bases:

>100_bases
GCGTTGGTTTTCCTATCAATGAGGATGTCAACGCAGTATTTGATATTTGCAGCATCTTTATCTTTTATCCAACACAAGCT
ACCAACAAAAGGAATTTATC

Downstream 100 bases:

>100_bases
TCCATATGTATTTACTATCTCGCATAAATACTTATGCGAGATAGCCGGCTTAACCGCTAGACATATACAACAATTTAGCA
GTTAAACCTTTTCTTAATAA

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MAAQKELSGAGLRGQVAGKTALSTVGKSGSGLTYRGYDVSELADKCIFEEVAYMLLYGNLPTQSELDAYQTKLKGLRGLP
QALKDVLERIPADAHPMDVLRTGCSMLGNLEMETDFSQENDKTDRMLAVFPSIINYWYRFTHDNVRIETETDDDTIGGHF
LHLLKGEKPNELHTKVMNVSLILYAEHEFNASTFTARVCASTLSDIHSCITGAIGSLRGHLHGGANEAAMDMIEGFTSPD
QAESEMMAMLARKDKIMGFGHAIYSESDPRNVVIKGWAEKLAADVGDEVLYPVSVRCEEVMWREKKLFCNADFFHASAYH
FMGIPTKLFTPIFVCSRLTGWAAHVFEQRANNRIIRPSAEYTGEELRPVPEMSAR

Sequences:

>Translated_375_residues
MAAQKELSGAGLRGQVAGKTALSTVGKSGSGLTYRGYDVSELADKCIFEEVAYMLLYGNLPTQSELDAYQTKLKGLRGLP
QALKDVLERIPADAHPMDVLRTGCSMLGNLEMETDFSQENDKTDRMLAVFPSIINYWYRFTHDNVRIETETDDDTIGGHF
LHLLKGEKPNELHTKVMNVSLILYAEHEFNASTFTARVCASTLSDIHSCITGAIGSLRGHLHGGANEAAMDMIEGFTSPD
QAESEMMAMLARKDKIMGFGHAIYSESDPRNVVIKGWAEKLAADVGDEVLYPVSVRCEEVMWREKKLFCNADFFHASAYH
FMGIPTKLFTPIFVCSRLTGWAAHVFEQRANNRIIRPSAEYTGEELRPVPEMSAR
>Mature_374_residues
AAQKELSGAGLRGQVAGKTALSTVGKSGSGLTYRGYDVSELADKCIFEEVAYMLLYGNLPTQSELDAYQTKLKGLRGLPQ
ALKDVLERIPADAHPMDVLRTGCSMLGNLEMETDFSQENDKTDRMLAVFPSIINYWYRFTHDNVRIETETDDDTIGGHFL
HLLKGEKPNELHTKVMNVSLILYAEHEFNASTFTARVCASTLSDIHSCITGAIGSLRGHLHGGANEAAMDMIEGFTSPDQ
AESEMMAMLARKDKIMGFGHAIYSESDPRNVVIKGWAEKLAADVGDEVLYPVSVRCEEVMWREKKLFCNADFFHASAYHF
MGIPTKLFTPIFVCSRLTGWAAHVFEQRANNRIIRPSAEYTGEELRPVPEMSAR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=374, Percent_Identity=52.6737967914438, Blast_Score=403, Evalue=1e-114,
Organism=Escherichia coli, GI1786939, Length=357, Percent_Identity=31.0924369747899, Blast_Score=162, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6319850, Length=398, Percent_Identity=23.6180904522613, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324328, Length=399, Percent_Identity=21.8045112781955, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640126, Length=379, Percent_Identity=22.9551451187335, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24640124, Length=380, Percent_Identity=22.8947368421053, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 41692; Mature: 41561

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQKELSGAGLRGQVAGKTALSTVGKSGSGLTYRGYDVSELADKCIFEEVAYMLLYGNL
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCC
PTQSELDAYQTKLKGLRGLPQALKDVLERIPADAHPMDVLRTGCSMLGNLEMETDFSQEN
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DKTDRMLAVFPSIINYWYRFTHDNVRIETETDDDTIGGHFLHLLKGEKPNELHTKVMNVS
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHEEEE
LILYAEHEFNASTFTARVCASTLSDIHSCITGAIGSLRGHLHGGANEAAMDMIEGFTSPD
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
QAESEMMAMLARKDKIMGFGHAIYSESDPRNVVIKGWAEKLAADVGDEVLYPVSVRCEEV
HHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECHHHHHHHHCCCCEEECHHHHHHHH
MWREKKLFCNADFFHASAYHFMGIPTKLFTPIFVCSRLTGWAAHVFEQRANNRIIRPSAE
HHHHHHHEECCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHH
YTGEELRPVPEMSAR
HCCHHCCCCCCCCCC
>Mature Secondary Structure 
AAQKELSGAGLRGQVAGKTALSTVGKSGSGLTYRGYDVSELADKCIFEEVAYMLLYGNL
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCC
PTQSELDAYQTKLKGLRGLPQALKDVLERIPADAHPMDVLRTGCSMLGNLEMETDFSQEN
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DKTDRMLAVFPSIINYWYRFTHDNVRIETETDDDTIGGHFLHLLKGEKPNELHTKVMNVS
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHEEEE
LILYAEHEFNASTFTARVCASTLSDIHSCITGAIGSLRGHLHGGANEAAMDMIEGFTSPD
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
QAESEMMAMLARKDKIMGFGHAIYSESDPRNVVIKGWAEKLAADVGDEVLYPVSVRCEEV
HHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECHHHHHHHHCCCCEEECHHHHHHHH
MWREKKLFCNADFFHASAYHFMGIPTKLFTPIFVCSRLTGWAAHVFEQRANNRIIRPSAE
HHHHHHHEECCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHH
YTGEELRPVPEMSAR
HCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]