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The map label for this gene is prpD [H]

Identifier: 93006130

GI number: 93006130

Start: 1590050

End: 1591552

Strand: Reverse

Name: prpD [H]

Synonym: Pcryo_1302

Alternate gene names: 93006130

Gene position: 1591552-1590050 (Counterclockwise)

Preceding gene: 93006131

Following gene: 93006127

Centisome position: 52.01

GC content: 47.37

Gene sequence:

>1503_bases
ATGTCTACTGTGGAAAGCAATGTACGACCGGAGTATGATAGCGAGATTGAAAAAATCGCCGATTATGTACTCAATTACTC
TATAGATAACGACTCACCCAATAGCGCTGATGCTTGGAATACTGCTCGCTACTGCCTGATGGATACGCTTGGCTGCGGAC
TACTGGCATTACGTTTCCCTGAATGTACCAAGCATTTAGGTCCTGATTGCGCCGATCAAATTACTCCTAATGGCGCGCGT
GTCCCCGGCACCTCCTATCGACTAGACCCTATTAAAGCTGCCTTTGACATTGGCTGTATCGTGCGCTGGCTTGATTACAA
TGATACATGGCTCGCTGCCGAATGGGGTCACCCCTCTGACAATTTAGGGGCGATTTTGGCAGTGGCGGATTATATCAGCC
AGCAAAACGTCAGTCAGGGGCATGCACCTCTTATTATGAAAGACGTACTAGAAGCTATGATTATGGCACATGAGATTCAA
GGGGTGTTGGCATTAGACAACTCCTTTAATCGTGTGGGTCTTGATCATGTATTTTTAGTGAAGTTGGCATCGACCGCTGT
CGTTGCCAAACTTTATAAACTGCCACATGAGCGTATCATGGCTGCCATCTCTCAAGCTATCGTCGATGGTCAAGCGCTAC
GTACCTATCGTCATGCACCCAATGCTGGTAGTCGCAAATCATGGGCTGCAGGGGATGCGACCAGTCGAGCCGTACGTTTG
GTCGATATCACGCGCCGCGGCGAGATGGGCATTCCATCTGCATTGACAGCGCCGCAATGGGGCTTTTATGATGTGCTGTT
TAGCCATACTAATAAGGATTTGGCGCTTAAGCCTGAAAACGAGCGGTGCTTTACCTTCCAGCGCGAGTTTGGCAGCTATG
TGATGGAGAATATCTTATTTAAGATAAGCTTCCCTGCCGAGTTCCATGCGCAAACGGCTTGTGAGGCGGCCGTTATCTTG
CATCCACGTATTGATGACCGCATGGAAGACATTGAAAAAATCATCCTAACGACTCATGAGTCTGCTATTCGGATCATCTC
AAAGGAAGGGACGCTGGCCAATCCTGCAGATCGCGATCATTGTTTGCAATATATGGTCGCAGTGGCTTTACTCACCGGTG
ATTTGATGGCTGAAAACTATGAAGATGATTATCATGCCGAGCATCATATTTTGATTGATGGTCTGCGTCATAAGATGGTC
GTTGAAGAAGATGCCGGTTATTCAAAAGACTATCATGATCCAAGCAAGCGCTCGATTGCCAATGCGATACAGGTATTCTT
TAAAGATGGCAGTAGTACCGATAAGGTAGCGATTGAATACCCTATCGGTCATAAACGCCGCCGTACTGAGGGTTTGCCGA
TATTGGAGGCAAAGTTCCGTGCCAGTCTTGCCACGCGGTTTATCGACAGTCGCTGTACAGAGATTTTTGCATTGTGTAGC
GACCAACAGCGGTTAGAGCAAACACCAGTAAACGAATTTATGGATTTATTTGTCGCCTATTGA

Upstream 100 bases:

>100_bases
GGTTAGATTGTGAGTATGCTATTTTTGATAGCAGGTTTATTGATTCGTAATAGGATTGATAAGTCACTTATATATCTGAC
TCAAGGAAGAGGTAACGATT

Downstream 100 bases:

>100_bases
TCGCCTTAACTCGTCAATTTCTAATGAAGTAAGACTCATTCTAAAACTTACCACGGTATAGATTCCCCTGCCCAATCCAC
AAAACTCCCTGATTGGGCAG

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSTVESNVRPEYDSEIEKIADYVLNYSIDNDSPNSADAWNTARYCLMDTLGCGLLALRFPECTKHLGPDCADQITPNGAR
VPGTSYRLDPIKAAFDIGCIVRWLDYNDTWLAAEWGHPSDNLGAILAVADYISQQNVSQGHAPLIMKDVLEAMIMAHEIQ
GVLALDNSFNRVGLDHVFLVKLASTAVVAKLYKLPHERIMAAISQAIVDGQALRTYRHAPNAGSRKSWAAGDATSRAVRL
VDITRRGEMGIPSALTAPQWGFYDVLFSHTNKDLALKPENERCFTFQREFGSYVMENILFKISFPAEFHAQTACEAAVIL
HPRIDDRMEDIEKIILTTHESAIRIISKEGTLANPADRDHCLQYMVAVALLTGDLMAENYEDDYHAEHHILIDGLRHKMV
VEEDAGYSKDYHDPSKRSIANAIQVFFKDGSSTDKVAIEYPIGHKRRRTEGLPILEAKFRASLATRFIDSRCTEIFALCS
DQQRLEQTPVNEFMDLFVAY

Sequences:

>Translated_500_residues
MSTVESNVRPEYDSEIEKIADYVLNYSIDNDSPNSADAWNTARYCLMDTLGCGLLALRFPECTKHLGPDCADQITPNGAR
VPGTSYRLDPIKAAFDIGCIVRWLDYNDTWLAAEWGHPSDNLGAILAVADYISQQNVSQGHAPLIMKDVLEAMIMAHEIQ
GVLALDNSFNRVGLDHVFLVKLASTAVVAKLYKLPHERIMAAISQAIVDGQALRTYRHAPNAGSRKSWAAGDATSRAVRL
VDITRRGEMGIPSALTAPQWGFYDVLFSHTNKDLALKPENERCFTFQREFGSYVMENILFKISFPAEFHAQTACEAAVIL
HPRIDDRMEDIEKIILTTHESAIRIISKEGTLANPADRDHCLQYMVAVALLTGDLMAENYEDDYHAEHHILIDGLRHKMV
VEEDAGYSKDYHDPSKRSIANAIQVFFKDGSSTDKVAIEYPIGHKRRRTEGLPILEAKFRASLATRFIDSRCTEIFALCS
DQQRLEQTPVNEFMDLFVAY
>Mature_499_residues
STVESNVRPEYDSEIEKIADYVLNYSIDNDSPNSADAWNTARYCLMDTLGCGLLALRFPECTKHLGPDCADQITPNGARV
PGTSYRLDPIKAAFDIGCIVRWLDYNDTWLAAEWGHPSDNLGAILAVADYISQQNVSQGHAPLIMKDVLEAMIMAHEIQG
VLALDNSFNRVGLDHVFLVKLASTAVVAKLYKLPHERIMAAISQAIVDGQALRTYRHAPNAGSRKSWAAGDATSRAVRLV
DITRRGEMGIPSALTAPQWGFYDVLFSHTNKDLALKPENERCFTFQREFGSYVMENILFKISFPAEFHAQTACEAAVILH
PRIDDRMEDIEKIILTTHESAIRIISKEGTLANPADRDHCLQYMVAVALLTGDLMAENYEDDYHAEHHILIDGLRHKMVV
EEDAGYSKDYHDPSKRSIANAIQVFFKDGSSTDKVAIEYPIGHKRRRTEGLPILEAKFRASLATRFIDSRCTEIFALCSD
QQRLEQTPVNEFMDLFVAY

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=501, Percent_Identity=58.4830339321357, Blast_Score=598, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6325258, Length=507, Percent_Identity=50.4930966469428, Blast_Score=497, Evalue=1e-141,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 56034; Mature: 55903

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVESNVRPEYDSEIEKIADYVLNYSIDNDSPNSADAWNTARYCLMDTLGCGLLALRFP
CCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
ECTKHLGPDCADQITPNGARVPGTSYRLDPIKAAFDIGCIVRWLDYNDTWLAAEWGHPSD
HHHHHCCCCHHHHCCCCCCCCCCCCEECCHHHHHHHHHHEEEEECCCCCEEEEECCCCCC
NLGAILAVADYISQQNVSQGHAPLIMKDVLEAMIMAHEIQGVLALDNSFNRVGLDHVFLV
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHH
KLASTAVVAKLYKLPHERIMAAISQAIVDGQALRTYRHAPNAGSRKSWAAGDATSRAVRL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHHEEEE
VDITRRGEMGIPSALTAPQWGFYDVLFSHTNKDLALKPENERCFTFQREFGSYVMENILF
EEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHE
KISFPAEFHAQTACEAAVILHPRIDDRMEDIEKIILTTHESAIRIISKEGTLANPADRDH
EEECCHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCHHH
CLQYMVAVALLTGDLMAENYEDDYHAEHHILIDGLRHKMVVEEDAGYSKDYHDPSKRSIA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHEEEECCCCCCCCCCCCHHHHHH
NAIQVFFKDGSSTDKVAIEYPIGHKRRRTEGLPILEAKFRASLATRFIDSRCTEIFALCS
HHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DQQRLEQTPVNEFMDLFVAY
CHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
STVESNVRPEYDSEIEKIADYVLNYSIDNDSPNSADAWNTARYCLMDTLGCGLLALRFP
CCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
ECTKHLGPDCADQITPNGARVPGTSYRLDPIKAAFDIGCIVRWLDYNDTWLAAEWGHPSD
HHHHHCCCCHHHHCCCCCCCCCCCCEECCHHHHHHHHHHEEEEECCCCCEEEEECCCCCC
NLGAILAVADYISQQNVSQGHAPLIMKDVLEAMIMAHEIQGVLALDNSFNRVGLDHVFLV
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHH
KLASTAVVAKLYKLPHERIMAAISQAIVDGQALRTYRHAPNAGSRKSWAAGDATSRAVRL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHHEEEE
VDITRRGEMGIPSALTAPQWGFYDVLFSHTNKDLALKPENERCFTFQREFGSYVMENILF
EEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHE
KISFPAEFHAQTACEAAVILHPRIDDRMEDIEKIILTTHESAIRIISKEGTLANPADRDH
EEECCHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCHHH
CLQYMVAVALLTGDLMAENYEDDYHAEHHILIDGLRHKMVVEEDAGYSKDYHDPSKRSIA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHEEEECCCCCCCCCCCCHHHHHH
NAIQVFFKDGSSTDKVAIEYPIGHKRRRTEGLPILEAKFRASLATRFIDSRCTEIFALCS
HHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DQQRLEQTPVNEFMDLFVAY
CHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]