Click here to switch to the map view.

The map label for this gene is yfmE [H]

Identifier: 93006112

GI number: 93006112

Start: 1561426

End: 1562385

Strand: Direct

Name: yfmE [H]

Synonym: Pcryo_1284

Alternate gene names: 93006112

Gene position: 1561426-1562385 (Clockwise)

Preceding gene: 93006111

Following gene: 93006113

Centisome position: 51.03

GC content: 46.77

Gene sequence:

>960_bases
GTGGTACTGATTATTCTGATGCTATTCATCCTTAGTGTGAGCAGCATCTTTTTTGGTGCCAGTGAATTAACGCTAGCGCA
AATCTGGCAGCATTTACAGCAAGGATTTGGCGGCGACAAAGACTTTATTGTGTGGCAGCACCGATTGCCGCGCACGCTAT
TATCAATTTTAGTGGGGGCAAGCCTTGGTCTATCAGGATCATTAGTACAAGGGGTCATTCGTAACCCGTTAGCATCTCCT
GACTTGATGGGTATCAGTGCTGGCGCAGGGTTTTGCGCGACTTTGTTGCTGGTTATGCTTCCTGCGGCACCAACGTGGTG
GCTATCTGTCGCAGCTTTACTTGGCGGATTATTGGCGTTTAGTTCTATCATGCTGTTGGCACAGATATCACAGCCTACGC
CAGCACGTTTGGCATTGATTGGCGTTGCCATTAGTGCTTTTTTAGCCAGTGGGATTAATTTTCTATTGGTGATTTATCCT
GTAGAAATCAATACCGCAATGATTTGGTTAACTGGTAGCTTGTGGGGTCGCAGCTGGCAACATATTCCAATGTTAACGAT
AATATTTGCCGTATTAATGGTCATCAGTATGTATTTAGCATGGCGTTTAGATGTATTGGGACTTGGCGAGGAAACGGCAT
ATCATCTTGGTGTGTCAGTCAAGCCATTAGAATTTACTACATTATTTATCGCAGTGGTGATTGCGAGTTTGGCGGTGTCG
GTGGCAGGGACAGTCAGTTTTATTGGTTTACTCGCGCCGCATATCGCACGATTGCTCTTTGGGCATCAGCACCGCATCTT
GTTGCCAGCGTCAGCGCTGATAGGCGCGATCATTTTAGTCACCGCAGATGTATTGGCACGCAGTCTATTTGCGCCAATTG
AGCTACCAGCTGGCGTCTTAACCTCAGTGATCGGTGCGCCTTATTTTATCTTTTTGCTATCGCGCTATAAGGGGTGGTGA

Upstream 100 bases:

>100_bases
CCTATCCAGCAACAAAAGTAAGCAACGAATGACGATACAAACTAAAGCTTCTTCATCGCGCTCAAACTCACTATTTGCTC
GTAAATCGGCAAGTAGGGCG

Downstream 100 bases:

>100_bases
TGCTATCAGTGAATGAACTACAGCTTGGCTATCATGGCGGACAGACAGTTATCAAAAACTTGTCCCTTGAGTTGCCAGAA
AATAAAGTCATTGGACTCAT

Product: transport system permease

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: Ferric-citrate import system permease protein [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MVLIILMLFILSVSSIFFGASELTLAQIWQHLQQGFGGDKDFIVWQHRLPRTLLSILVGASLGLSGSLVQGVIRNPLASP
DLMGISAGAGFCATLLLVMLPAAPTWWLSVAALLGGLLAFSSIMLLAQISQPTPARLALIGVAISAFLASGINFLLVIYP
VEINTAMIWLTGSLWGRSWQHIPMLTIIFAVLMVISMYLAWRLDVLGLGEETAYHLGVSVKPLEFTTLFIAVVIASLAVS
VAGTVSFIGLLAPHIARLLFGHQHRILLPASALIGAIILVTADVLARSLFAPIELPAGVLTSVIGAPYFIFLLSRYKGW

Sequences:

>Translated_319_residues
MVLIILMLFILSVSSIFFGASELTLAQIWQHLQQGFGGDKDFIVWQHRLPRTLLSILVGASLGLSGSLVQGVIRNPLASP
DLMGISAGAGFCATLLLVMLPAAPTWWLSVAALLGGLLAFSSIMLLAQISQPTPARLALIGVAISAFLASGINFLLVIYP
VEINTAMIWLTGSLWGRSWQHIPMLTIIFAVLMVISMYLAWRLDVLGLGEETAYHLGVSVKPLEFTTLFIAVVIASLAVS
VAGTVSFIGLLAPHIARLLFGHQHRILLPASALIGAIILVTADVLARSLFAPIELPAGVLTSVIGAPYFIFLLSRYKGW
>Mature_319_residues
MVLIILMLFILSVSSIFFGASELTLAQIWQHLQQGFGGDKDFIVWQHRLPRTLLSILVGASLGLSGSLVQGVIRNPLASP
DLMGISAGAGFCATLLLVMLPAAPTWWLSVAALLGGLLAFSSIMLLAQISQPTPARLALIGVAISAFLASGINFLLVIYP
VEINTAMIWLTGSLWGRSWQHIPMLTIIFAVLMVISMYLAWRLDVLGLGEETAYHLGVSVKPLEFTTLFIAVVIASLAVS
VAGTVSFIGLLAPHIARLLFGHQHRILLPASALIGAIILVTADVLARSLFAPIELPAGVLTSVIGAPYFIFLLSRYKGW

Specific function: Part of the ABC transporter complex yfmCDEF involved in citrate-dependent Fe(3+) import. Involved in the translocation of the substrate across the membrane (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790740, Length=281, Percent_Identity=43.0604982206406, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1790741, Length=317, Percent_Identity=34.384858044164, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786347, Length=278, Percent_Identity=32.0143884892086, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786804, Length=303, Percent_Identity=36.3036303630363, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788004, Length=287, Percent_Identity=37.9790940766551, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786805, Length=311, Percent_Identity=32.7974276527331, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000522 [H]

Pfam domain/function: PF01032 FecCD [H]

EC number: NA

Molecular weight: Translated: 34162; Mature: 34162

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLIILMLFILSVSSIFFGASELTLAQIWQHLQQGFGGDKDFIVWQHRLPRTLLSILVGA
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHH
SLGLSGSLVQGVIRNPLASPDLMGISAGAGFCATLLLVMLPAAPTWWLSVAALLGGLLAF
CCCCCHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SSIMLLAQISQPTPARLALIGVAISAFLASGINFLLVIYPVEINTAMIWLTGSLWGRSWQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECEEEEEEEECHHCCCCC
HIPMLTIIFAVLMVISMYLAWRLDVLGLGEETAYHLGVSVKPLEFTTLFIAVVIASLAVS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECCEECCHHHHHHHHHHHHHHHHHH
VAGTVSFIGLLAPHIARLLFGHQHRILLPASALIGAIILVTADVLARSLFAPIELPAGVL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TSVIGAPYFIFLLSRYKGW
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVLIILMLFILSVSSIFFGASELTLAQIWQHLQQGFGGDKDFIVWQHRLPRTLLSILVGA
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHH
SLGLSGSLVQGVIRNPLASPDLMGISAGAGFCATLLLVMLPAAPTWWLSVAALLGGLLAF
CCCCCHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SSIMLLAQISQPTPARLALIGVAISAFLASGINFLLVIYPVEINTAMIWLTGSLWGRSWQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECEEEEEEEECHHCCCCC
HIPMLTIIFAVLMVISMYLAWRLDVLGLGEETAYHLGVSVKPLEFTTLFIAVVIASLAVS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECCEECCHHHHHHHHHHHHHHHHHH
VAGTVSFIGLLAPHIARLLFGHQHRILLPASALIGAIILVTADVLARSLFAPIELPAGVL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TSVIGAPYFIFLLSRYKGW
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]