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The map label for this gene is alkK [H]

Identifier: 93006065

GI number: 93006065

Start: 1507153

End: 1508847

Strand: Direct

Name: alkK [H]

Synonym: Pcryo_1237

Alternate gene names: 93006065

Gene position: 1507153-1508847 (Clockwise)

Preceding gene: 93006064

Following gene: 93006066

Centisome position: 49.26

GC content: 43.54

Gene sequence:

>1695_bases
ATGACTACGATACTTAACCAACATGAGGATTACCAGCAAGCTCAAGAAGCCTATGGTTTTCCTTTGATTATCAAACAGCT
ACTAAACCGTGCAAAAATCGCCTCTAAAGATCAAACCATTAGTTATGCGGATAAGGTTACTTATACGTATTCTGAATTTT
TTAAGCGTGTTAATCGCTTGGCAAACGTACTTAAAAACATGGGTTTGCAAGCAGGTGATGTGGTTGCTGTTATGGATTGG
GACAGTCATCGTTACCTTGAAGCGTACTTTGCTGTGCCTATGTCTGGAATGATTTTGCAAACGGTCAATGTACGCTTGGC
TGAAGATAAAGTGCTGTATACCATCAATCATTCTAAACCAAAAGCTTTGTTACTTAATGCTGAGTTTGAGCCAATGGCTA
AAAACTACCGTCATGAAGCCCCTTCTATTGAGAAAATTATTTGGTTGGATGATGCTGAGTATGATGAAGACACTATAAAA
GCTCAGCAAACTAGCATGCCAGATTATGTTGAAGGTGAGTATGAGGCAATGCTAGCCGCTGCCAGTGATGAGTTTGATTT
TCCCGATTTTGATGAAAATACCATTGCTACCACATTTTATACCAGTGGTACGACGGGTGACCCAAAAGGGGTTTTCTTTA
CCCATCGACAGATCGTATTACAGACGCTATCGAGTACGCTTGCCTCTGCACTCAGCGCCGAAGGTCAAGGTGCTCGCTAT
AATGATGTTTATATGCCAATGACGCCATTGTTCCATGTGCATGCTTGGTGCTGGCCGTATGGGGCAACCATGATAGGGCT
AAAACAAGTGTATCCAGGGCGCTATGTATCCGAAGTATTGGTTGACTTGATTGAACAGCATAAGGTAACGCTATCACATG
GTGTCCCAACCATTTTACAAATGTTAATCAAAGAAATGGCAACCCGAGGACGCAAGTTTAACGGTCTTAAATTGTCAGTA
GGGGGCTCCAAATTAAACGAAGCTCTAGCTGAAGCCGCTATTGAAAGTGGCATAGAGTTCATCTCGGGGTTTGGAATGTC
AGAGTCCTGTCCTGTCCTTGCCAGAATGGCATTTGGTGATCAGACAAGTACTATGACAACGACAGAGCAGATCAACTATC
GTTGTCTTTCTGGCTCACCAATTATGCTGGTATCGATGGAGCTTTGGGATGCGAACGGCAAATCTTTACCGATGGATGGT
GAGTCGACCGGCGAACTCGTCGTTCGAGCGCCTTGGCTGACCCAAAGCTATTTTAAGAATCCAGATGCTGGCGATGAGTT
ATGGCGTGGTGGCTGGATGCATACCCAAGATATTGCCTGCATTACCGCTGATGGTACTCTAAAAATTACGGATAGACTCA
AAGATGTCATCAAGTCAGGTGGCGAGTGGGTATCCTCGCTTGAAGTCGAAAATATTTTATCATTCCATCCTTGCGTTGCT
GAAGTGGCCGTTATTGGAGTAGCGGATGAAAAATGGGGCGAACGTCCTCTAGCGTTAGTGGTCCTGAAACCTGATCATAC
CGATATTAAAGCGGAGGAAATTTTAGCATTGGGTCATCAAGCGGTTGAAAAAGGCTATTTACCAAAGTATGGCGTCCCAA
GTGAAATAAAGTTCTTAAGAGAAATGCCGAAGACCAGTGTGGGTAAGCTTGATAAGAAAAAGATGCGGATGATGTACGCA
GAAAAATTAATCTAA

Upstream 100 bases:

>100_bases
TTATGCATTGGCATTGGGCAAGGGCTTGCCATGGTGATTGAACGAGTCTAACCCTTTCAATACTTTTGCCATATATTTTT
TATTATCAAGGATGACCATA

Downstream 100 bases:

>100_bases
TCCAAACATATTTATTAGTAACTGCAAGAAAACAGTTAATGCAGCTTACTGATAATTTGAGTAGGTATGTTCATTGGCGT
GACTAGACGAGATAATTATG

Product: AMP-dependent synthetase and ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MTTILNQHEDYQQAQEAYGFPLIIKQLLNRAKIASKDQTISYADKVTYTYSEFFKRVNRLANVLKNMGLQAGDVVAVMDW
DSHRYLEAYFAVPMSGMILQTVNVRLAEDKVLYTINHSKPKALLLNAEFEPMAKNYRHEAPSIEKIIWLDDAEYDEDTIK
AQQTSMPDYVEGEYEAMLAAASDEFDFPDFDENTIATTFYTSGTTGDPKGVFFTHRQIVLQTLSSTLASALSAEGQGARY
NDVYMPMTPLFHVHAWCWPYGATMIGLKQVYPGRYVSEVLVDLIEQHKVTLSHGVPTILQMLIKEMATRGRKFNGLKLSV
GGSKLNEALAEAAIESGIEFISGFGMSESCPVLARMAFGDQTSTMTTTEQINYRCLSGSPIMLVSMELWDANGKSLPMDG
ESTGELVVRAPWLTQSYFKNPDAGDELWRGGWMHTQDIACITADGTLKITDRLKDVIKSGGEWVSSLEVENILSFHPCVA
EVAVIGVADEKWGERPLALVVLKPDHTDIKAEEILALGHQAVEKGYLPKYGVPSEIKFLREMPKTSVGKLDKKKMRMMYA
EKLI

Sequences:

>Translated_564_residues
MTTILNQHEDYQQAQEAYGFPLIIKQLLNRAKIASKDQTISYADKVTYTYSEFFKRVNRLANVLKNMGLQAGDVVAVMDW
DSHRYLEAYFAVPMSGMILQTVNVRLAEDKVLYTINHSKPKALLLNAEFEPMAKNYRHEAPSIEKIIWLDDAEYDEDTIK
AQQTSMPDYVEGEYEAMLAAASDEFDFPDFDENTIATTFYTSGTTGDPKGVFFTHRQIVLQTLSSTLASALSAEGQGARY
NDVYMPMTPLFHVHAWCWPYGATMIGLKQVYPGRYVSEVLVDLIEQHKVTLSHGVPTILQMLIKEMATRGRKFNGLKLSV
GGSKLNEALAEAAIESGIEFISGFGMSESCPVLARMAFGDQTSTMTTTEQINYRCLSGSPIMLVSMELWDANGKSLPMDG
ESTGELVVRAPWLTQSYFKNPDAGDELWRGGWMHTQDIACITADGTLKITDRLKDVIKSGGEWVSSLEVENILSFHPCVA
EVAVIGVADEKWGERPLALVVLKPDHTDIKAEEILALGHQAVEKGYLPKYGVPSEIKFLREMPKTSVGKLDKKKMRMMYA
EKLI
>Mature_563_residues
TTILNQHEDYQQAQEAYGFPLIIKQLLNRAKIASKDQTISYADKVTYTYSEFFKRVNRLANVLKNMGLQAGDVVAVMDWD
SHRYLEAYFAVPMSGMILQTVNVRLAEDKVLYTINHSKPKALLLNAEFEPMAKNYRHEAPSIEKIIWLDDAEYDEDTIKA
QQTSMPDYVEGEYEAMLAAASDEFDFPDFDENTIATTFYTSGTTGDPKGVFFTHRQIVLQTLSSTLASALSAEGQGARYN
DVYMPMTPLFHVHAWCWPYGATMIGLKQVYPGRYVSEVLVDLIEQHKVTLSHGVPTILQMLIKEMATRGRKFNGLKLSVG
GSKLNEALAEAAIESGIEFISGFGMSESCPVLARMAFGDQTSTMTTTEQINYRCLSGSPIMLVSMELWDANGKSLPMDGE
STGELVVRAPWLTQSYFKNPDAGDELWRGGWMHTQDIACITADGTLKITDRLKDVIKSGGEWVSSLEVENILSFHPCVAE
VAVIGVADEKWGERPLALVVLKPDHTDIKAEEILALGHQAVEKGYLPKYGVPSEIKFLREMPKTSVGKLDKKKMRMMYAE
KLI

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=590, Percent_Identity=26.1016949152542, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI187761343, Length=590, Percent_Identity=26.1016949152542, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI156151445, Length=561, Percent_Identity=23.8859180035651, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI42544132, Length=562, Percent_Identity=23.4875444839858, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI122937307, Length=533, Percent_Identity=25.328330206379, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI38505220, Length=524, Percent_Identity=24.618320610687, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI58082049, Length=520, Percent_Identity=22.8846153846154, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI157311624, Length=520, Percent_Identity=23.0769230769231, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311622, Length=520, Percent_Identity=23.0769230769231, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI115511026, Length=526, Percent_Identity=22.4334600760456, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI28416953, Length=561, Percent_Identity=22.9946524064171, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI221142682, Length=515, Percent_Identity=25.4368932038835, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788107, Length=564, Percent_Identity=25.531914893617, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI145693145, Length=520, Percent_Identity=26.3461538461538, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786810, Length=524, Percent_Identity=23.8549618320611, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1788595, Length=520, Percent_Identity=24.2307692307692, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790505, Length=546, Percent_Identity=23.992673992674, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=545, Percent_Identity=24.7706422018349, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=558, Percent_Identity=24.910394265233, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17559526, Length=537, Percent_Identity=25.1396648044693, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71996755, Length=532, Percent_Identity=25.7518796992481, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=521, Percent_Identity=22.2648752399232, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=567, Percent_Identity=23.2804232804233, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=567, Percent_Identity=23.2804232804233, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=567, Percent_Identity=23.2804232804233, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=464, Percent_Identity=24.7844827586207, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=569, Percent_Identity=23.5500878734622, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17558820, Length=526, Percent_Identity=24.1444866920152, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=519, Percent_Identity=23.3140655105973, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17556552, Length=518, Percent_Identity=24.7104247104247, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=369, Percent_Identity=23.0352303523035, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI115534440, Length=555, Percent_Identity=21.6216216216216, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17551278, Length=529, Percent_Identity=23.2514177693762, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=390, Percent_Identity=26.4102564102564, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319264, Length=552, Percent_Identity=24.0942028985507, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21355181, Length=562, Percent_Identity=24.3772241992883, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI18859661, Length=533, Percent_Identity=24.390243902439, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24581924, Length=585, Percent_Identity=23.5897435897436, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19922652, Length=572, Percent_Identity=20.979020979021, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356947, Length=543, Percent_Identity=23.0202578268877, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648260, Length=540, Percent_Identity=24.2592592592593, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24653035, Length=533, Percent_Identity=22.3264540337711, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI62471681, Length=605, Percent_Identity=21.9834710743802, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI62471687, Length=605, Percent_Identity=21.9834710743802, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24586634, Length=605, Percent_Identity=21.9834710743802, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI22026970, Length=605, Percent_Identity=21.9834710743802, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI62471679, Length=606, Percent_Identity=22.2772277227723, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI62471683, Length=606, Percent_Identity=22.2772277227723, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI62471685, Length=606, Percent_Identity=22.2772277227723, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24586636, Length=606, Percent_Identity=22.2772277227723, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI62471689, Length=606, Percent_Identity=22.2772277227723, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667955, Length=562, Percent_Identity=24.0213523131673, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI62472339, Length=560, Percent_Identity=24.1071428571429, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI161076582, Length=543, Percent_Identity=22.4677716390424, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648257, Length=551, Percent_Identity=22.3230490018149, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24667959, Length=435, Percent_Identity=24.5977011494253, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356441, Length=475, Percent_Identity=21.4736842105263, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20130357, Length=526, Percent_Identity=21.6730038022814, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 63066; Mature: 62935

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTILNQHEDYQQAQEAYGFPLIIKQLLNRAKIASKDQTISYADKVTYTYSEFFKRVNRL
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEHHHHHEEHHHHHHHHHHHH
ANVLKNMGLQAGDVVAVMDWDSHRYLEAYFAVPMSGMILQTVNVRLAEDKVLYTINHSKP
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEEEEEECCEEEEEEECCCC
KALLLNAEFEPMAKNYRHEAPSIEKIIWLDDAEYDEDTIKAQQTSMPDYVEGEYEAMLAA
CEEEEECCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHEEE
ASDEFDFPDFDENTIATTFYTSGTTGDPKGVFFTHRQIVLQTLSSTLASALSAEGQGARY
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCC
NDVYMPMTPLFHVHAWCWPYGATMIGLKQVYPGRYVSEVLVDLIEQHKVTLSHGVPTILQ
CCEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEHHCCHHHHHH
MLIKEMATRGRKFNGLKLSVGGSKLNEALAEAAIESGIEFISGFGMSESCPVLARMAFGD
HHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCC
QTSTMTTTEQINYRCLSGSPIMLVSMELWDANGKSLPMDGESTGELVVRAPWLTQSYFKN
CCCCEEEHHHCCEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHCCC
PDAGDELWRGGWMHTQDIACITADGTLKITDRLKDVIKSGGEWVSSLEVENILSFHPCVA
CCCCHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHH
EVAVIGVADEKWGERPLALVVLKPDHTDIKAEEILALGHQAVEKGYLPKYGVPSEIKFLR
HHEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
EMPKTSVGKLDKKKMRMMYAEKLI
HCCCCHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTILNQHEDYQQAQEAYGFPLIIKQLLNRAKIASKDQTISYADKVTYTYSEFFKRVNRL
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEHHHHHEEHHHHHHHHHHHH
ANVLKNMGLQAGDVVAVMDWDSHRYLEAYFAVPMSGMILQTVNVRLAEDKVLYTINHSKP
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEEEEEECCEEEEEEECCCC
KALLLNAEFEPMAKNYRHEAPSIEKIIWLDDAEYDEDTIKAQQTSMPDYVEGEYEAMLAA
CEEEEECCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHEEE
ASDEFDFPDFDENTIATTFYTSGTTGDPKGVFFTHRQIVLQTLSSTLASALSAEGQGARY
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCC
NDVYMPMTPLFHVHAWCWPYGATMIGLKQVYPGRYVSEVLVDLIEQHKVTLSHGVPTILQ
CCEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEHHCCHHHHHH
MLIKEMATRGRKFNGLKLSVGGSKLNEALAEAAIESGIEFISGFGMSESCPVLARMAFGD
HHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCC
QTSTMTTTEQINYRCLSGSPIMLVSMELWDANGKSLPMDGESTGELVVRAPWLTQSYFKN
CCCCEEEHHHCCEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHCCC
PDAGDELWRGGWMHTQDIACITADGTLKITDRLKDVIKSGGEWVSSLEVENILSFHPCVA
CCCCHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHH
EVAVIGVADEKWGERPLALVVLKPDHTDIKAEEILALGHQAVEKGYLPKYGVPSEIKFLR
HHEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
EMPKTSVGKLDKKKMRMMYAEKLI
HCCCCHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]