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The map label for this gene is xthA [H]

Identifier: 93006020

GI number: 93006020

Start: 1444477

End: 1445301

Strand: Direct

Name: xthA [H]

Synonym: Pcryo_1192

Alternate gene names: 93006020

Gene position: 1444477-1445301 (Clockwise)

Preceding gene: 93006016

Following gene: 93006021

Centisome position: 47.21

GC content: 43.27

Gene sequence:

>825_bases
ATGACCCGTTTTGTTTGTTTTAATATCAATGGTATCCGAGCTCGTCAACATCAGCTGGAGGCGGTGCGAGATATTATCGA
TCCTGATGTCATGGGTCTGCAAGAAACCAAGGTGCATGATGAGCAGTTTCCCCTTGAAAATGTAGAAGGCTTGGGCTATC
ACGTCGAGTATTTTGGACAGAAAGCGCATTACGGTGTGGCATTATTATCGAAAGTTGCGCCTATTTTTGTGCAAAAGGGT
TTCCCTAACGAAGATGAAGAGGCGCAAAAACGTTTTATTCATGCGCGTTATGAGTTTGATGGGCGTGAGATTGATGTGCT
CAATGGCTATTTCCCACAGGGTGAGAGTCAAGATCATCCGACCAAATTTCCGATGAAACGTGCTTATTATGCTGATTTAA
TGGCCTATATTGATACGCTAAAAGCAGAAGGGCGCTCGCTAGTGATTATGGGGGATATGAATATTGCCCCAGAAGACATC
GATATTGGAATCGGTGAAGTAAATGCTAAGCGCTGGTTGAAAAATAGAAAAACTTCCTTCCTACCTGAGGAGCGTGAGTG
GTATAGCAATCTTATGTCACGTGAGTTGACTGATACCTACCGTTTGCATTATCCAGAAAGCACCGAATTATACAGCTGGT
TTGATTATCGCAGTCGCGGTTTTGATGATGATCCTAAGCGTGGATTGCGTATTGATCATATTTTATGTACGCCTGATCTT
GTTGAATGTTGTGTTGATGCGGGTATCAGCTATGAGCTGCGCGCTATGGAAAAACCCTCTGACCATGCGCCTATTTGGAC
AGCATTTGATTTAAACAAAGTATGA

Upstream 100 bases:

>100_bases
TATCATAACCGTATTTGTTTTATTTTAGATTTATTTATAAGTGCATTTTAAAACTGCATTTACTTATCAAAGCAGTTATT
TATATAGGATAGGATAATTT

Downstream 100 bases:

>100_bases
TGTTTTTACTATTACGACTAAGGCTTTACAATCAATAAAAATATACCAATATCAATAATTAAGGAGTGAATGATGGCAGT
TGGTAATGTTGCAGTACCTG

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTRFVCFNINGIRARQHQLEAVRDIIDPDVMGLQETKVHDEQFPLENVEGLGYHVEYFGQKAHYGVALLSKVAPIFVQKG
FPNEDEEAQKRFIHARYEFDGREIDVLNGYFPQGESQDHPTKFPMKRAYYADLMAYIDTLKAEGRSLVIMGDMNIAPEDI
DIGIGEVNAKRWLKNRKTSFLPEEREWYSNLMSRELTDTYRLHYPESTELYSWFDYRSRGFDDDPKRGLRIDHILCTPDL
VECCVDAGISYELRAMEKPSDHAPIWTAFDLNKV

Sequences:

>Translated_274_residues
MTRFVCFNINGIRARQHQLEAVRDIIDPDVMGLQETKVHDEQFPLENVEGLGYHVEYFGQKAHYGVALLSKVAPIFVQKG
FPNEDEEAQKRFIHARYEFDGREIDVLNGYFPQGESQDHPTKFPMKRAYYADLMAYIDTLKAEGRSLVIMGDMNIAPEDI
DIGIGEVNAKRWLKNRKTSFLPEEREWYSNLMSRELTDTYRLHYPESTELYSWFDYRSRGFDDDPKRGLRIDHILCTPDL
VECCVDAGISYELRAMEKPSDHAPIWTAFDLNKV
>Mature_273_residues
TRFVCFNINGIRARQHQLEAVRDIIDPDVMGLQETKVHDEQFPLENVEGLGYHVEYFGQKAHYGVALLSKVAPIFVQKGF
PNEDEEAQKRFIHARYEFDGREIDVLNGYFPQGESQDHPTKFPMKRAYYADLMAYIDTLKAEGRSLVIMGDMNIAPEDID
IGIGEVNAKRWLKNRKTSFLPEEREWYSNLMSRELTDTYRLHYPESTELYSWFDYRSRGFDDDPKRGLRIDHILCTPDLV
ECCVDAGISYELRAMEKPSDHAPIWTAFDLNKV

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=59.9250936329588, Blast_Score=336, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI71989536, Length=276, Percent_Identity=25, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI221330655, Length=276, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17136678, Length=276, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31900; Mature: 31769

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFVCFNINGIRARQHQLEAVRDIIDPDVMGLQETKVHDEQFPLENVEGLGYHVEYFGQ
CCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCEEHHHHCC
KAHYGVALLSKVAPIFVQKGFPNEDEEAQKRFIHARYEFDGREIDVLNGYFPQGESQDHP
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCCCCC
TKFPMKRAYYADLMAYIDTLKAEGRSLVIMGDMNIAPEDIDIGIGEVNAKRWLKNRKTSF
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCC
LPEEREWYSNLMSRELTDTYRLHYPESTELYSWFDYRSRGFDDDPKRGLRIDHILCTPDL
CCCHHHHHHHHHHHHHCHHEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCHHH
VECCVDAGISYELRAMEKPSDHAPIWTAFDLNKV
HHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
TRFVCFNINGIRARQHQLEAVRDIIDPDVMGLQETKVHDEQFPLENVEGLGYHVEYFGQ
CEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCEEHHHHCC
KAHYGVALLSKVAPIFVQKGFPNEDEEAQKRFIHARYEFDGREIDVLNGYFPQGESQDHP
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCCCCC
TKFPMKRAYYADLMAYIDTLKAEGRSLVIMGDMNIAPEDIDIGIGEVNAKRWLKNRKTSF
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCC
LPEEREWYSNLMSRELTDTYRLHYPESTELYSWFDYRSRGFDDDPKRGLRIDHILCTPDL
CCCHHHHHHHHHHHHHCHHEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCHHH
VECCVDAGISYELRAMEKPSDHAPIWTAFDLNKV
HHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]