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The map label for this gene is trmL [H]

Identifier: 93005998

GI number: 93005998

Start: 1417435

End: 1417995

Strand: Direct

Name: trmL [H]

Synonym: Pcryo_1170

Alternate gene names: 93005998

Gene position: 1417435-1417995 (Clockwise)

Preceding gene: 93005996

Following gene: 93006003

Centisome position: 46.32

GC content: 45.28

Gene sequence:

>561_bases
ATGACCATTCATATCGTGCTGGTAGCGCCAAAAATGCCCAGTAACACGGGCAATATCATTCGTCTGTGTGCCAATAGTGG
GGCGCAGCTGCATTTAGTCAAACCATTAGGGTTTGAGCTGGATGATAAAAAATTGCGCCGTGCAGGATTAGATTATCATG
AGTATGCCCATCTGCAAGTACACGACAATTGGTCAATAGCAAAGCAAGCATTGCAGGATGCTGACTGTCATACCATTACG
GCATTGACCACCAAGTTAAGTAAGCCTTTTTATGATTATGATTTTGGTGGACAAGAGACTGCAGAGTCGGGCAGTGATTT
TTCTGGAAATAATGTGGCCATGGTTTTTGGCTCAGAAACAGCGGGACTGGCAGATGATATACGTGAAGATATTGGCGCTG
ATAACTGGCTACGATTGCCGATGCTAGCAGATTCACGCAGTTTGAATCTGTCGAACAGCGTGGCAATATGTTTATATGAG
GTATGGCGACAACAAGGATTTGGCGGTGATGAAGGTCGTAGTATCGGCTATGAGACACTCATCGTCTACGACCCAAAGTA
G

Upstream 100 bases:

>100_bases
CCCAGTATCAAAATGTAATTATTGGCAGTAAACTGTAACTTTTAATTACCTTTGCCATATAAGTCAGAAAAATAACCCTA
ATGCTAATGACGGAGAGAGT

Downstream 100 bases:

>100_bases
TACTTTTATTATAAAAATGCTCATAAAAAAGGCAGATAAATAAAGAATTTATCTGTCTTTTTTATGAGCATTTATAAGCA
ATTCGGCGGTTTGGGTAGCC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MTIHIVLVAPKMPSNTGNIIRLCANSGAQLHLVKPLGFELDDKKLRRAGLDYHEYAHLQVHDNWSIAKQALQDADCHTIT
ALTTKLSKPFYDYDFGGQETAESGSDFSGNNVAMVFGSETAGLADDIREDIGADNWLRLPMLADSRSLNLSNSVAICLYE
VWRQQGFGGDEGRSIGYETLIVYDPK

Sequences:

>Translated_186_residues
MTIHIVLVAPKMPSNTGNIIRLCANSGAQLHLVKPLGFELDDKKLRRAGLDYHEYAHLQVHDNWSIAKQALQDADCHTIT
ALTTKLSKPFYDYDFGGQETAESGSDFSGNNVAMVFGSETAGLADDIREDIGADNWLRLPMLADSRSLNLSNSVAICLYE
VWRQQGFGGDEGRSIGYETLIVYDPK
>Mature_185_residues
TIHIVLVAPKMPSNTGNIIRLCANSGAQLHLVKPLGFELDDKKLRRAGLDYHEYAHLQVHDNWSIAKQALQDADCHTITA
LTTKLSKPFYDYDFGGQETAESGSDFSGNNVAMVFGSETAGLADDIREDIGADNWLRLPMLADSRSLNLSNSVAICLYEV
WRQQGFGGDEGRSIGYETLIVYDPK

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=167, Percent_Identity=43.1137724550898, Blast_Score=142, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 20536; Mature: 20405

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIHIVLVAPKMPSNTGNIIRLCANSGAQLHLVKPLGFELDDKKLRRAGLDYHEYAHLQV
CEEEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCHHHEEEEEE
HDNWSIAKQALQDADCHTITALTTKLSKPFYDYDFGGQETAESGSDFSGNNVAMVFGSET
ECCHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
AGLADDIREDIGADNWLRLPMLADSRSLNLSNSVAICLYEVWRQQGFGGDEGRSIGYETL
CCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEE
IVYDPK
EEECCC
>Mature Secondary Structure 
TIHIVLVAPKMPSNTGNIIRLCANSGAQLHLVKPLGFELDDKKLRRAGLDYHEYAHLQV
EEEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCHHHEEEEEE
HDNWSIAKQALQDADCHTITALTTKLSKPFYDYDFGGQETAESGSDFSGNNVAMVFGSET
ECCHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
AGLADDIREDIGADNWLRLPMLADSRSLNLSNSVAICLYEVWRQQGFGGDEGRSIGYETL
CCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEE
IVYDPK
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA