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The map label for this gene is lipB

Identifier: 93005912

GI number: 93005912

Start: 1304633

End: 1305490

Strand: Reverse

Name: lipB

Synonym: Pcryo_1084

Alternate gene names: 93005912

Gene position: 1305490-1304633 (Counterclockwise)

Preceding gene: 93005918

Following gene: 93005911

Centisome position: 42.66

GC content: 45.22

Gene sequence:

>858_bases
ATGCCAAATACTATGCAGGCGCTTCATACGCGACCACTTAATGAAAATCTCATCACAAAGTCACTCACTGCTGCTGACTA
TGTGGCTACTCTTGATGCCATGCTATCACGCACGTTAGAGCGTATCTCTCTTAAAAAAGAGCAAGGACTGCGTACGCCTG
ACGAGTTATGGATTGTCGATCACAATGACGTTTATACCCTTGGGCAAGCAGGTAAAGAAGAGCATATCCTGCAACGTACT
AATACGCCTATCATCAAAACGGATCGCGGTGGTCAGGTAACATGGCATGGACATGGTCAGCTGGTTATGTATTGGCTGTT
TGATTTGGACAGTATCAGCTGGAGTGTGCGCAATATGGTCTCACACGCTGAGCAAGCGATTGAGGATGTGGTCAATGACT
GTCTCAAGAGCACAGCATCAACTGACACCAACCATATCAGCGCCCGTGCCCGCCGTGATGCCCCAGGTGTCTATCTATAT
GCTGATATGGCTACAGAAACGGACAGCGCTCCTACTAGTGATGAGATAGAAGTTGATAATACCATTATGATTGGCAAAAT
CGCGTCGCTTGGCTTTAAAATCAAGCATGGCTTTAGCTACCATGGTATTGCCATTAATTTAGATTGTGATTTATCAGCAT
TTAATGCTATCAACCCTTGTGGTTATGCTGGCATGCAAATGTTGCGTTTGGCGGATTTTGTTAATATGAACCAAGCGACC
AATATACAGCCAAATAACCTACCGCTTACCGATGACAAAAAAACTCTTACCTACGAGCAATTTACGCAACAGCTCATCGA
CAATATCGCCCAGCGTCATGCAGGAGACATCCCATTGCGTGAATTAGCGCCAAAATAG

Upstream 100 bases:

>100_bases
AAAATTTGGGCGGCAATATGAAAACTGCTACACTCAATGACAGTAATATTAACAATAGATAGCCCATTTTACATAACAAA
AACATAAGACAGCCACTCTC

Downstream 100 bases:

>100_bases
GTATTTTTTGCATAATCCTTTTTTGCATAATATCGTGCGCGCTTAGACTTAAGCATTTTGAGAAAATTTGATTCAAACTT
GTTATAATCGCACACGGTAC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MPNTMQALHTRPLNENLITKSLTAADYVATLDAMLSRTLERISLKKEQGLRTPDELWIVDHNDVYTLGQAGKEEHILQRT
NTPIIKTDRGGQVTWHGHGQLVMYWLFDLDSISWSVRNMVSHAEQAIEDVVNDCLKSTASTDTNHISARARRDAPGVYLY
ADMATETDSAPTSDEIEVDNTIMIGKIASLGFKIKHGFSYHGIAINLDCDLSAFNAINPCGYAGMQMLRLADFVNMNQAT
NIQPNNLPLTDDKKTLTYEQFTQQLIDNIAQRHAGDIPLRELAPK

Sequences:

>Translated_285_residues
MPNTMQALHTRPLNENLITKSLTAADYVATLDAMLSRTLERISLKKEQGLRTPDELWIVDHNDVYTLGQAGKEEHILQRT
NTPIIKTDRGGQVTWHGHGQLVMYWLFDLDSISWSVRNMVSHAEQAIEDVVNDCLKSTASTDTNHISARARRDAPGVYLY
ADMATETDSAPTSDEIEVDNTIMIGKIASLGFKIKHGFSYHGIAINLDCDLSAFNAINPCGYAGMQMLRLADFVNMNQAT
NIQPNNLPLTDDKKTLTYEQFTQQLIDNIAQRHAGDIPLRELAPK
>Mature_284_residues
PNTMQALHTRPLNENLITKSLTAADYVATLDAMLSRTLERISLKKEQGLRTPDELWIVDHNDVYTLGQAGKEEHILQRTN
TPIIKTDRGGQVTWHGHGQLVMYWLFDLDSISWSVRNMVSHAEQAIEDVVNDCLKSTASTDTNHISARARRDAPGVYLYA
DMATETDSAPTSDEIEVDNTIMIGKIASLGFKIKHGFSYHGIAINLDCDLSAFNAINPCGYAGMQMLRLADFVNMNQATN
IQPNNLPLTDDKKTLTYEQFTQQLIDNIAQRHAGDIPLRELAPK

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Escherichia coli, GI87081767, Length=196, Percent_Identity=40.3061224489796, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_PSYCK (Q1QBT8)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_580349.1
- ProteinModelPortal:   Q1QBT8
- STRING:   Q1QBT8
- GeneID:   4034693
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_1084
- NMPDR:   fig|335284.3.peg.497
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DNTIMIG
- PhylomeDB:   Q1QBT8
- ProtClustDB:   CLSK839245
- BioCyc:   PCRY335284:PCRYO_1084-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 31854; Mature: 31723

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNTMQALHTRPLNENLITKSLTAADYVATLDAMLSRTLERISLKKEQGLRTPDELWIVD
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
HNDVYTLGQAGKEEHILQRTNTPIIKTDRGGQVTWHGHGQLVMYWLFDLDSISWSVRNMV
CCCEEECCCCCCHHHHHHHCCCCEEEECCCCEEEEECCCCEEEEEEEHHCHHHHHHHHHH
SHAEQAIEDVVNDCLKSTASTDTNHISARARRDAPGVYLYADMATETDSAPTSDEIEVDN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEECC
TIMIGKIASLGFKIKHGFSYHGIAINLDCDLSAFNAINPCGYAGMQMLRLADFVNMNQAT
EEEEEEHHHCCEEEECCCEECCEEEEEECCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
NIQPNNLPLTDDKKTLTYEQFTQQLIDNIAQRHAGDIPLRELAPK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure 
PNTMQALHTRPLNENLITKSLTAADYVATLDAMLSRTLERISLKKEQGLRTPDELWIVD
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
HNDVYTLGQAGKEEHILQRTNTPIIKTDRGGQVTWHGHGQLVMYWLFDLDSISWSVRNMV
CCCEEECCCCCCHHHHHHHCCCCEEEECCCCEEEEECCCCEEEEEEEHHCHHHHHHHHHH
SHAEQAIEDVVNDCLKSTASTDTNHISARARRDAPGVYLYADMATETDSAPTSDEIEVDN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEECC
TIMIGKIASLGFKIKHGFSYHGIAINLDCDLSAFNAINPCGYAGMQMLRLADFVNMNQAT
EEEEEEHHHCCEEEECCCEECCEEEEEECCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
NIQPNNLPLTDDKKTLTYEQFTQQLIDNIAQRHAGDIPLRELAPK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA