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The map label for this gene is aceF [H]

Identifier: 93005879

GI number: 93005879

Start: 1263327

End: 1265069

Strand: Direct

Name: aceF [H]

Synonym: Pcryo_1051

Alternate gene names: 93005879

Gene position: 1263327-1265069 (Clockwise)

Preceding gene: 93005878

Following gene: 93005881

Centisome position: 41.29

GC content: 44.75

Gene sequence:

>1743_bases
ATGGACATTAAAGCCCCCGATTTGGGTGTCGATAGCGCCGAAGTCAGTGAAATTATGGTTGCAGTTGGTGATGTCATTGC
AAAAGATGATAATATTATTTTACTTGAGTCAGACAAGGCTTCGGTCGAAGTGCCAAGCTCTGCTGCTGGTAAAGTGACTA
AAATCAGTGTTGCGATTGGTGATCAAGTCAGTGAAGGTATGGTGCTAATCGAGCTTGAAAGCGAGACTAATAGTCAAGAT
GAAAGTCAAAGCGTACAAACACCTGCTGCTGACACTCAAGAGAAATCTCTTGATACTGAGCAAGAAGAACCGCAAAAAGA
AGAGCAGAGCGATAAGGTAGCCGTTGAGTCAAATACTGATCAACCTGTTGAGCAAGAAGCAACAGCGACCACGCATGCGC
TCCCAGATTTAGGCGTCGATGAAGCGCAAGTATCTGAGATTATGGTCAGTGTTGGCGATATGGTCACTGCTGATCAGTCT
ATCTTGCTAATCGAGTCAGATAAAGCGTCGGTTGAAGTACCAGCGCCACAAGCCGGCAAAGTTGAGAAAATACTGATACA
AGTAGGCGATATGGTTGCCAATGGGCAAGACTTTATTGTCATCATTGGTCAAAGCTCAGACAATGCTAATGCTACGAGTG
AAGCCAAAGCACCAGTTGCTCAATCGCAAGATTCAAAACCAGCAGTGAGCGAGCAAAAGACTGATTCAAGTCAGGAAGCT
GCTAAACAAGCTACGACTGAGCCTAAACAAGTTGCTAGTACTACTGCAAAAAGCAGTACAGGTAATAAGCTGACCGAAGC
ACAAGTCAATGAAAAAATGGTTGATGTCTATGCTGGTCCCGCCGTGCGTAAACTTGCCCGTCAGTTGGGTGTGGATATTA
GCCAAGTAACAGGTTCAGCACTGAATGCACGTATCTTAAAAGAAGATTTGTTTGCTCATGTGAAGCAAAGCTTGACGACA
AAACAAGCTGCACCCGCTACTGGAGCCGGTGTATCTAGAGCCAGCTTACCAAGCTTACCTGATATGAGTAATGTTGAAAT
TTGGGGCGAAACAGAAACCCAAGACTTAACTCGCTTGCAAAAGGTGTCGATTCCACAGCTGAATTACAACACTTACTTGC
CACAGGTGACGCAGTTTGATTTGTCTGATATCACTGAGACAGAGCAGCTGCGTAGTGAGCTTAAAGGCAGTATGAAAGCT
GAAGGTGTTGGACTGACAATTTTGGCCTTTATTGTCAAAGCAACGGCTTATGCATTGACCCAGCATCCACGTTTTAACAG
TCATTTAAGTGACGACAATACCCAAGTCATCCTACGTAAAAGCGTAAATATGGGGATTGCAGTAGCAACAGATGATGGCT
TAATCGTGCCAGTGATTAAAAATGCTCACGAAAAAGGTATCAAGCAAATTGCGATTGAAATCGGCGAATTGGCCATCAAA
GCCCGTGATAAAAAGCTAAGTACTAAAGACTTGCAAGGCGCAAGCTTTACCATCTCAAGTCAAGGTATCTTAGGTGGTAC
GGCGTTTACGCCATTGGTAAACTGGCCACAAGTTGGTATTTTGGGGGCATCAGAAGCGACTATGCAACCGAAATGGAATG
CTGCCAAGCAAACTTTTGAGCCGCGCTTGATGTTGCCATTGTCATTATCATATGATCATCGTGTGATTAATGGGGCTGAT
GCGGCAGTATTCACGCGTTATGTCGCAACATTGCTTGCTGACCCGCGCCGTATTTTGCTATAG

Upstream 100 bases:

>100_bases
GAAATCAGCAGTGAAGGTCGCGCCGAAGATCAAGCCGATGCAACCTTACTTAATAATGATGATGTCGAAGAAAAATAACC
ATAAGACAAGGAAATGAAAA

Downstream 100 bases:

>100_bases
GTTTTATTCTTATTTAAATTTTATATAAAAGAAAGACTATCTGCGGATGGTCTTTTTTTATCTCTGTTATTTGATTAGAA
TATAGATAAATAACAGACAC

Product: dehydrogenase catalytic domain-containing protein

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MDIKAPDLGVDSAEVSEIMVAVGDVIAKDDNIILLESDKASVEVPSSAAGKVTKISVAIGDQVSEGMVLIELESETNSQD
ESQSVQTPAADTQEKSLDTEQEEPQKEEQSDKVAVESNTDQPVEQEATATTHALPDLGVDEAQVSEIMVSVGDMVTADQS
ILLIESDKASVEVPAPQAGKVEKILIQVGDMVANGQDFIVIIGQSSDNANATSEAKAPVAQSQDSKPAVSEQKTDSSQEA
AKQATTEPKQVASTTAKSSTGNKLTEAQVNEKMVDVYAGPAVRKLARQLGVDISQVTGSALNARILKEDLFAHVKQSLTT
KQAAPATGAGVSRASLPSLPDMSNVEIWGETETQDLTRLQKVSIPQLNYNTYLPQVTQFDLSDITETEQLRSELKGSMKA
EGVGLTILAFIVKATAYALTQHPRFNSHLSDDNTQVILRKSVNMGIAVATDDGLIVPVIKNAHEKGIKQIAIEIGELAIK
ARDKKLSTKDLQGASFTISSQGILGGTAFTPLVNWPQVGILGASEATMQPKWNAAKQTFEPRLMLPLSLSYDHRVINGAD
AAVFTRYVATLLADPRRILL

Sequences:

>Translated_580_residues
MDIKAPDLGVDSAEVSEIMVAVGDVIAKDDNIILLESDKASVEVPSSAAGKVTKISVAIGDQVSEGMVLIELESETNSQD
ESQSVQTPAADTQEKSLDTEQEEPQKEEQSDKVAVESNTDQPVEQEATATTHALPDLGVDEAQVSEIMVSVGDMVTADQS
ILLIESDKASVEVPAPQAGKVEKILIQVGDMVANGQDFIVIIGQSSDNANATSEAKAPVAQSQDSKPAVSEQKTDSSQEA
AKQATTEPKQVASTTAKSSTGNKLTEAQVNEKMVDVYAGPAVRKLARQLGVDISQVTGSALNARILKEDLFAHVKQSLTT
KQAAPATGAGVSRASLPSLPDMSNVEIWGETETQDLTRLQKVSIPQLNYNTYLPQVTQFDLSDITETEQLRSELKGSMKA
EGVGLTILAFIVKATAYALTQHPRFNSHLSDDNTQVILRKSVNMGIAVATDDGLIVPVIKNAHEKGIKQIAIEIGELAIK
ARDKKLSTKDLQGASFTISSQGILGGTAFTPLVNWPQVGILGASEATMQPKWNAAKQTFEPRLMLPLSLSYDHRVINGAD
AAVFTRYVATLLADPRRILL
>Mature_580_residues
MDIKAPDLGVDSAEVSEIMVAVGDVIAKDDNIILLESDKASVEVPSSAAGKVTKISVAIGDQVSEGMVLIELESETNSQD
ESQSVQTPAADTQEKSLDTEQEEPQKEEQSDKVAVESNTDQPVEQEATATTHALPDLGVDEAQVSEIMVSVGDMVTADQS
ILLIESDKASVEVPAPQAGKVEKILIQVGDMVANGQDFIVIIGQSSDNANATSEAKAPVAQSQDSKPAVSEQKTDSSQEA
AKQATTEPKQVASTTAKSSTGNKLTEAQVNEKMVDVYAGPAVRKLARQLGVDISQVTGSALNARILKEDLFAHVKQSLTT
KQAAPATGAGVSRASLPSLPDMSNVEIWGETETQDLTRLQKVSIPQLNYNTYLPQVTQFDLSDITETEQLRSELKGSMKA
EGVGLTILAFIVKATAYALTQHPRFNSHLSDDNTQVILRKSVNMGIAVATDDGLIVPVIKNAHEKGIKQIAIEIGELAIK
ARDKKLSTKDLQGASFTISSQGILGGTAFTPLVNWPQVGILGASEATMQPKWNAAKQTFEPRLMLPLSLSYDHRVINGAD
AAVFTRYVATLLADPRRILL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=451, Percent_Identity=29.490022172949, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI19923748, Length=206, Percent_Identity=35.4368932038835, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI31711992, Length=440, Percent_Identity=28.8636363636364, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI203098816, Length=175, Percent_Identity=34.8571428571429, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI203098753, Length=175, Percent_Identity=34.8571428571429, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI260898739, Length=143, Percent_Identity=36.3636363636364, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786305, Length=311, Percent_Identity=44.6945337620579, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI1786946, Length=451, Percent_Identity=27.9379157427938, Blast_Score=148, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=434, Percent_Identity=29.4930875576037, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=32.1428571428571, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=452, Percent_Identity=26.9911504424779, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=189, Percent_Identity=31.2169312169312, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320352, Length=476, Percent_Identity=27.5210084033613, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324258, Length=436, Percent_Identity=25.6880733944954, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859875, Length=456, Percent_Identity=27.6315789473684, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24645909, Length=201, Percent_Identity=34.3283582089552, Blast_Score=123, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24582497, Length=217, Percent_Identity=31.7972350230415, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129315, Length=217, Percent_Identity=31.7972350230415, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 61848; Mature: 61848

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKAPDLGVDSAEVSEIMVAVGDVIAKDDNIILLESDKASVEVPSSAAGKVTKISVAIG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCCCEEEEEEEEC
DQVSEGMVLIELESETNSQDESQSVQTPAADTQEKSLDTEQEEPQKEEQSDKVAVESNTD
CCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCHHHHCCCCEEEECCCC
QPVEQEATATTHALPDLGVDEAQVSEIMVSVGDMVTADQSILLIESDKASVEVPAPQAGK
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEECCCEEEEEECCCCEEECCCCCCCC
VEKILIQVGDMVANGQDFIVIIGQSSDNANATSEAKAPVAQSQDSKPAVSEQKTDSSQEA
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHH
AKQATTEPKQVASTTAKSSTGNKLTEAQVNEKMVDVYAGPAVRKLARQLGVDISQVTGSA
HHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCHH
LNARILKEDLFAHVKQSLTTKQAAPATGAGVSRASLPSLPDMSNVEIWGETETQDLTRLQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHH
KVSIPQLNYNTYLPQVTQFDLSDITETEQLRSELKGSMKAEGVGLTILAFIVKATAYALT
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
QHPRFNSHLSDDNTQVILRKSVNMGIAVATDDGLIVPVIKNAHEKGIKQIAIEIGELAIK
CCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCEEEHHHHCCHHHHHHHHHHHHHHHHEE
ARDKKLSTKDLQGASFTISSQGILGGTAFTPLVNWPQVGILGASEATMQPKWNAAKQTFE
CCCCCCCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCEEEECCCCCCCCCCCCHHHHCCC
PRLMLPLSLSYDHRVINGADAAVFTRYVATLLADPRRILL
CEEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure
MDIKAPDLGVDSAEVSEIMVAVGDVIAKDDNIILLESDKASVEVPSSAAGKVTKISVAIG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCCCEEEEEEEEC
DQVSEGMVLIELESETNSQDESQSVQTPAADTQEKSLDTEQEEPQKEEQSDKVAVESNTD
CCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCHHHHCCCCEEEECCCC
QPVEQEATATTHALPDLGVDEAQVSEIMVSVGDMVTADQSILLIESDKASVEVPAPQAGK
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEECCCEEEEEECCCCEEECCCCCCCC
VEKILIQVGDMVANGQDFIVIIGQSSDNANATSEAKAPVAQSQDSKPAVSEQKTDSSQEA
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCHHHH
AKQATTEPKQVASTTAKSSTGNKLTEAQVNEKMVDVYAGPAVRKLARQLGVDISQVTGSA
HHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHCCHH
LNARILKEDLFAHVKQSLTTKQAAPATGAGVSRASLPSLPDMSNVEIWGETETQDLTRLQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHH
KVSIPQLNYNTYLPQVTQFDLSDITETEQLRSELKGSMKAEGVGLTILAFIVKATAYALT
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
QHPRFNSHLSDDNTQVILRKSVNMGIAVATDDGLIVPVIKNAHEKGIKQIAIEIGELAIK
CCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCEEEHHHHCCHHHHHHHHHHHHHHHHEE
ARDKKLSTKDLQGASFTISSQGILGGTAFTPLVNWPQVGILGASEATMQPKWNAAKQTFE
CCCCCCCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCEEEECCCCCCCCCCCCHHHHCCC
PRLMLPLSLSYDHRVINGADAAVFTRYVATLLADPRRILL
CEEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]