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The map label for this gene is cobS [H]

Identifier: 93005855

GI number: 93005855

Start: 1227685

End: 1228518

Strand: Direct

Name: cobS [H]

Synonym: Pcryo_1027

Alternate gene names: 93005855

Gene position: 1227685-1228518 (Clockwise)

Preceding gene: 93005854

Following gene: 93005856

Centisome position: 40.12

GC content: 45.2

Gene sequence:

>834_bases
ATGCAAAGTTCATCCTCAAAGCTGTCTGTATTGCAGCGCCTTCGTTATGAAGCCCGATTGATATTGGTTGCTGTTCAGTT
TTTAACCCGTATATCCGTCCCGTCTTTTAGCCATTATGATCCGCAGTGGCTGCACCAAAGCAGCCGTTATTTTCCAGCGG
TAGGCTTACTGATAGGTTTGCTTTGTGCTGGCGTGTTTTGGCTTAGCAGTCTCTTATTCATACCGCTAGTTGCGGCAGTG
ATAAGTACGGCTTTTGGTATCAAGCTGACAGGTGCTTTTCATGAAGATGGACTTGCCGATAGCTGCGATGGTTTAGGTGG
TGGGCTAACTCGCGAGCGTACACTCACGATTATGAAAGACTCACGCCTAGGCACTTATGGCGTATTAGGTTTGGTCTCAG
CGCTATTGCTTAAAATTAGCTTACTTACTGCGATGCCAACACCCGTTGCTATCGTTGCACTCATTATTGGGCATACTGCG
TCACGTTTATTTTGTATTAGCTTACTGACGCTATTGCCTTATGGCGGAGAGATTGAACATGCCAAAGCAAAACCGATGGC
ACAGCAGCTGACACCATTACAAGCTATATATAGCAGTGGTTGGCTAATATTGGCTATTACTTTTGTCACACTGGTTTTCC
CTAATACCATGCAACAGATTGGTATTTGGCAATGGTTATTGGCTTTATTGCTAGGCATAGGCGCGACAGATTATATGCGG
CGCTTATTACGTAGACGTTTAGATGGTTATACGGGTGATGGCTTAGGAGCCACGCAGCAGCTTAGCGAAATTGCTATTTA
CATTGGACTGGCCGCTTCATTGCCCTTAATTTAA

Upstream 100 bases:

>100_bases
GTGGTGCGGCATTAGCGTATCCACTATTACAATCTGCCTGTGCCATTATCAATGAGATGGCAAGTTTTAGTGATGCTGGT
ATCAGTGAGCAAAGCAAGTG

Downstream 100 bases:

>100_bases
GCCAAAGCGTTAAAAATGATGCTAAGAAAGGATACTCGCACTCATGATTCTGTATATTTGGCGTCATCCAAAACCTATTA
CGGCTGGTGGTCTCTGTATC

Product: cobalamin-5-phosphate synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MQSSSSKLSVLQRLRYEARLILVAVQFLTRISVPSFSHYDPQWLHQSSRYFPAVGLLIGLLCAGVFWLSSLLFIPLVAAV
ISTAFGIKLTGAFHEDGLADSCDGLGGGLTRERTLTIMKDSRLGTYGVLGLVSALLLKISLLTAMPTPVAIVALIIGHTA
SRLFCISLLTLLPYGGEIEHAKAKPMAQQLTPLQAIYSSGWLILAITFVTLVFPNTMQQIGIWQWLLALLLGIGATDYMR
RLLRRRLDGYTGDGLGATQQLSEIAIYIGLAASLPLI

Sequences:

>Translated_277_residues
MQSSSSKLSVLQRLRYEARLILVAVQFLTRISVPSFSHYDPQWLHQSSRYFPAVGLLIGLLCAGVFWLSSLLFIPLVAAV
ISTAFGIKLTGAFHEDGLADSCDGLGGGLTRERTLTIMKDSRLGTYGVLGLVSALLLKISLLTAMPTPVAIVALIIGHTA
SRLFCISLLTLLPYGGEIEHAKAKPMAQQLTPLQAIYSSGWLILAITFVTLVFPNTMQQIGIWQWLLALLLGIGATDYMR
RLLRRRLDGYTGDGLGATQQLSEIAIYIGLAASLPLI
>Mature_277_residues
MQSSSSKLSVLQRLRYEARLILVAVQFLTRISVPSFSHYDPQWLHQSSRYFPAVGLLIGLLCAGVFWLSSLLFIPLVAAV
ISTAFGIKLTGAFHEDGLADSCDGLGGGLTRERTLTIMKDSRLGTYGVLGLVSALLLKISLLTAMPTPVAIVALIIGHTA
SRLFCISLLTLLPYGGEIEHAKAKPMAQQLTPLQAIYSSGWLILAITFVTLVFPNTMQQIGIWQWLLALLLGIGATDYMR
RLLRRRLDGYTGDGLGATQQLSEIAIYIGLAASLPLI

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=263, Percent_Identity=31.1787072243346, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 30072; Mature: 30072

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSSSSKLSVLQRLRYEARLILVAVQFLTRISVPSFSHYDPQWLHQSSRYFPAVGLLIGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHH
LCAGVFWLSSLLFIPLVAAVISTAFGIKLTGAFHEDGLADSCDGLGGGLTRERTLTIMKD
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCHHHEEEEEEC
SRLGTYGVLGLVSALLLKISLLTAMPTPVAIVALIIGHTASRLFCISLLTLLPYGGEIEH
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
AKAKPMAQQLTPLQAIYSSGWLILAITFVTLVFPNTMQQIGIWQWLLALLLGIGATDYMR
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHH
RLLRRRLDGYTGDGLGATQQLSEIAIYIGLAASLPLI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQSSSSKLSVLQRLRYEARLILVAVQFLTRISVPSFSHYDPQWLHQSSRYFPAVGLLIGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHH
LCAGVFWLSSLLFIPLVAAVISTAFGIKLTGAFHEDGLADSCDGLGGGLTRERTLTIMKD
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCHHHEEEEEEC
SRLGTYGVLGLVSALLLKISLLTAMPTPVAIVALIIGHTASRLFCISLLTLLPYGGEIEH
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
AKAKPMAQQLTPLQAIYSSGWLILAITFVTLVFPNTMQQIGIWQWLLALLLGIGATDYMR
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHH
RLLRRRLDGYTGDGLGATQQLSEIAIYIGLAASLPLI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA