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The map label for this gene is nemR [H]

Identifier: 93005842

GI number: 93005842

Start: 1213577

End: 1214215

Strand: Reverse

Name: nemR [H]

Synonym: Pcryo_1014

Alternate gene names: 93005842

Gene position: 1214215-1213577 (Counterclockwise)

Preceding gene: 93005844

Following gene: 93005841

Centisome position: 39.68

GC content: 44.44

Gene sequence:

>639_bases
ATGTCTATACCCAATTCTATAAAACATACTCAACCAGATAATTCGCTATCTAATATTGCACTATCAAACACTAGAGACCA
TCTGTTAACGACGGGCTATAAGCTCATCGCACAAAAAGGCTTTACCGCAGTTGGCATTAAGCAAATATTAGATACGGCAG
GCGTGCCGAAAGGCTCTTTTTATCATTATTTTGCGTCAAAAGAAGCCTTTGGCGAGGCGATTATCAGCCACTACTTTACC
CAGTATAAGCAGCGCCTTGATACTATTGGTTCACAAGATGTTAGTGCCCAGCAGAAATTGTATAACTATTTCCAAAACTG
GTATGACACACAGCAAAGCGGCTGCGCTCATGAGAAATGTTTGGTGGTAAAGCTTAGCGCTGAAGTGTCAGATATGTCGG
AGCCAATGCGTAAAGTCCTATATGCGGGCTATCAGCAAACGATTACATGGCTTGCAACTCAAATCAAAGCTGGCTGGGCA
GACGGTTCTGTGCCTCATCCCGCTAACGTAGCGGCTGAGAGTATGGCAAAACGTTGGTATTTTGCATGGCTTGGTGCCAG
CTTAGTGACCAAAATTAGTCAAACGGATACCCCATTAGCAGAAGTATGGCTGATGACTACTACCGCTATGGGTCGCTAG

Upstream 100 bases:

>100_bases
AACCTGTTGTTTGTTGAACAAACGTAACTTGCCAATCAATACTTATAAATTTGTTAAATCATCTTAAATAATTAGTCTCT
CTAAGGAGAACATAAGTCAC

Downstream 100 bases:

>100_bases
TTTTTTCATTTTATAAACGATCTATTTACGTTCTGTAACTCTCGTTTTGTCTATCCTTCTGTCTTACTCCTCCTTGTTTA
ACTTACCATGAACTAGTGTT

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSIPNSIKHTQPDNSLSNIALSNTRDHLLTTGYKLIAQKGFTAVGIKQILDTAGVPKGSFYHYFASKEAFGEAIISHYFT
QYKQRLDTIGSQDVSAQQKLYNYFQNWYDTQQSGCAHEKCLVVKLSAEVSDMSEPMRKVLYAGYQQTITWLATQIKAGWA
DGSVPHPANVAAESMAKRWYFAWLGASLVTKISQTDTPLAEVWLMTTTAMGR

Sequences:

>Translated_212_residues
MSIPNSIKHTQPDNSLSNIALSNTRDHLLTTGYKLIAQKGFTAVGIKQILDTAGVPKGSFYHYFASKEAFGEAIISHYFT
QYKQRLDTIGSQDVSAQQKLYNYFQNWYDTQQSGCAHEKCLVVKLSAEVSDMSEPMRKVLYAGYQQTITWLATQIKAGWA
DGSVPHPANVAAESMAKRWYFAWLGASLVTKISQTDTPLAEVWLMTTTAMGR
>Mature_211_residues
SIPNSIKHTQPDNSLSNIALSNTRDHLLTTGYKLIAQKGFTAVGIKQILDTAGVPKGSFYHYFASKEAFGEAIISHYFTQ
YKQRLDTIGSQDVSAQQKLYNYFQNWYDTQQSGCAHEKCLVVKLSAEVSDMSEPMRKVLYAGYQQTITWLATQIKAGWAD
GSVPHPANVAAESMAKRWYFAWLGASLVTKISQTDTPLAEVWLMTTTAMGR

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=198, Percent_Identity=39.8989898989899, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 23644; Mature: 23513

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPNSIKHTQPDNSLSNIALSNTRDHLLTTGYKLIAQKGFTAVGIKQILDTAGVPKGSF
CCCCCCCCCCCCCCCHHHEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCH
YHYFASKEAFGEAIISHYFTQYKQRLDTIGSQDVSAQQKLYNYFQNWYDTQQSGCAHEKC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
LVVKLSAEVSDMSEPMRKVLYAGYQQTITWLATQIKAGWADGSVPHPANVAAESMAKRWY
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
FAWLGASLVTKISQTDTPLAEVWLMTTTAMGR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIPNSIKHTQPDNSLSNIALSNTRDHLLTTGYKLIAQKGFTAVGIKQILDTAGVPKGSF
CCCCCCCCCCCCCCHHHEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCH
YHYFASKEAFGEAIISHYFTQYKQRLDTIGSQDVSAQQKLYNYFQNWYDTQQSGCAHEKC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCE
LVVKLSAEVSDMSEPMRKVLYAGYQQTITWLATQIKAGWADGSVPHPANVAAESMAKRWY
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
FAWLGASLVTKISQTDTPLAEVWLMTTTAMGR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]