Click here to switch to the map view.

The map label for this gene is kefA [H]

Identifier: 93005832

GI number: 93005832

Start: 1199764

End: 1201710

Strand: Direct

Name: kefA [H]

Synonym: Pcryo_1004

Alternate gene names: 93005832

Gene position: 1199764-1201710 (Clockwise)

Preceding gene: 93005831

Following gene: 93005833

Centisome position: 39.21

GC content: 44.79

Gene sequence:

>1947_bases
ATGCAAACTCCTTTCGGATTTCTCAGGACTCACTCTCTACAGTCTCATGCCCAGTTTCTATCGCGACTGCTCTTTATAAT
TGGCAGCTTATTTATCCTATTAAATATGGCGTGCATTTCTGCTTATGCAGCAAGCAGTACTGAGGAAATAGACAGTCGAA
ATATTGAAGAGACAGTTACTACAAAACCGGAAAATTTGGTTGAGTATGCTGCGCAAAGAATCGACAAGTTAAAAGAGGAA
GGAGTGACTGTCTCTGCCGTTGCAGCAGAGGTAATTAGCCCGCTAGAAAAAAGGACAGTGCAGCAAGCAGGTGTGCTACA
AGGAGATTCTGGTCTCACTGGTGATTACAGCGAAAACTACTATGCGTTAGATAAGCTCAATGCAGGTTTGCCGCCCTTGT
CTGAGCCGCCAAACTTGTCAACCCCACTTGCCACATTAGAGTTTTTCCAATCGGCAGTCATGAAGCAGCAATATGATTTG
GCAGCTTATGCACTAAATATGAACTTGATTGATAGAGAATCACAGTCGAATCAAGCAATGGATTTGGTCAAACAGCTTGA
TTTTTTATTGGTTGAAAAGCAGCTATATGTGTTTGATAAGTTGCCTGATCGTCCTGATGGCTTGATTGAACCGCCGCTTG
GCAGTACGAGCAGCATTCAAGGGATTCCTAGACGTTCTATTCAGCTTGGTTATATTGATTATCGCGAGCGCCGAGTACCC
TTGCATCTAGAGCGTGTACGTATTGATAATGGGGCGCCTTTTTGGGTATTTTCAGCGCAGACGGTGGCAAATATTGATAA
GCTTTATGAACAATATCATCCGGCAAAGTTTGAGCGATATCTGCCTAGTTGGACCAAGCTAAAGCTTTTTAATATTGCTA
TCTGGGAGTTTTTAGCGTTATTTATATTCTTCTCCTTTACCATGGGCATTGGCTGGCTGCTAAGTACTGCCGCTGGCAAG
CTCCTCAATTGGTATGTCATGGATAAAGAAGGCAACCGTCTTGGCACCCTTCATCACAACGGTGTAGAGGATTTGGTCAA
TAAGCTGACGGTCCCTCTAACCTTTACCATCAGCTTCTCATCGGTATTTACCTTGGTATCGGGCGGCTTCCCTTACTTGG
ATGCAGTCGCTTCCTCTATACGTCCGATTATTTGGATTGGATTGGTCTTTAGCGCCATGTGGCTCGGTATACGTGTTATC
AACTTTTTTGCCAACCGCTATCAAGATATGCAGATTGAAAATCTAGGAGAGGAGCAGTTTGACAAAGTGCGCCGTCGTCG
AACTTACGTATCGATATTTCGTCGCGTCTTCGTATTTGTCATGGTGTTGGGCAGTATTTGGATAGGTCTGAGTGAGTTTG
CCAACATGGAAGGCTTTGGTAAAACCTTACTGACTTCGGCTGGCATTGCTGGCGTGGTCATTGGTATCGCCGCTCAACCA
ATATTGGGTAACATCATTGCTGGAGTACAGGTAGCAGTGACCCAGCCCGTACGAATTGGCGACAGTGTGATAATGGATGG
CAACTTTAGTACCGTTGAAGACCTGCGTTACACTTATGCGGTACTAAAAACATGGGATGAACGTCGATTAATCGTGCCGA
TGCGCGAATTGATCACCGAAATGGTAGAGAACTGGTCGCATACAGAAGTACATCAGACCTGTCCTGTGTTCTTATATATC
GATTACGGCGCAGATATTGACGCCATTCGGCAGCAGTTTATTTCGGTGGTGAAAGACAACAAGCTTTGGGACAACAAGAC
TGAGCCTGAAATATTTGTCGTTGAGGTAACTGAAAAGACCATTCAACTGCGTGGCGCTGTTTCAGCGGTAGGCCCTGTAG
AAGCATGGACACTGGCCTGTGAGATACGTGAGCAGATGCTTGATTATTTGTACCGTGAACAAAAGCATTATTTACCAGCT
GAGCACCTTGTATTGAGACAAAAATAG

Upstream 100 bases:

>100_bases
TGTTCATTCACAAACAATAATAAATTAAGCCAGCTATCTGCTGGCTTTTTTGTGTCTTCTGCATTAGCTTAATATCATAC
TTATTAGCAAGAGTTTTTGT

Downstream 100 bases:

>100_bases
TCAATGTCTATTGGTTTAGCGCTTAAAAAATTTGAATGTACAGCGTCACTATCGATAGACAGCTTTGGGTAAAACCTATT
GACAAATATAGCATGCGATT

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MQTPFGFLRTHSLQSHAQFLSRLLFIIGSLFILLNMACISAYAASSTEEIDSRNIEETVTTKPENLVEYAAQRIDKLKEE
GVTVSAVAAEVISPLEKRTVQQAGVLQGDSGLTGDYSENYYALDKLNAGLPPLSEPPNLSTPLATLEFFQSAVMKQQYDL
AAYALNMNLIDRESQSNQAMDLVKQLDFLLVEKQLYVFDKLPDRPDGLIEPPLGSTSSIQGIPRRSIQLGYIDYRERRVP
LHLERVRIDNGAPFWVFSAQTVANIDKLYEQYHPAKFERYLPSWTKLKLFNIAIWEFLALFIFFSFTMGIGWLLSTAAGK
LLNWYVMDKEGNRLGTLHHNGVEDLVNKLTVPLTFTISFSSVFTLVSGGFPYLDAVASSIRPIIWIGLVFSAMWLGIRVI
NFFANRYQDMQIENLGEEQFDKVRRRRTYVSIFRRVFVFVMVLGSIWIGLSEFANMEGFGKTLLTSAGIAGVVIGIAAQP
ILGNIIAGVQVAVTQPVRIGDSVIMDGNFSTVEDLRYTYAVLKTWDERRLIVPMRELITEMVENWSHTEVHQTCPVFLYI
DYGADIDAIRQQFISVVKDNKLWDNKTEPEIFVVEVTEKTIQLRGAVSAVGPVEAWTLACEIREQMLDYLYREQKHYLPA
EHLVLRQK

Sequences:

>Translated_648_residues
MQTPFGFLRTHSLQSHAQFLSRLLFIIGSLFILLNMACISAYAASSTEEIDSRNIEETVTTKPENLVEYAAQRIDKLKEE
GVTVSAVAAEVISPLEKRTVQQAGVLQGDSGLTGDYSENYYALDKLNAGLPPLSEPPNLSTPLATLEFFQSAVMKQQYDL
AAYALNMNLIDRESQSNQAMDLVKQLDFLLVEKQLYVFDKLPDRPDGLIEPPLGSTSSIQGIPRRSIQLGYIDYRERRVP
LHLERVRIDNGAPFWVFSAQTVANIDKLYEQYHPAKFERYLPSWTKLKLFNIAIWEFLALFIFFSFTMGIGWLLSTAAGK
LLNWYVMDKEGNRLGTLHHNGVEDLVNKLTVPLTFTISFSSVFTLVSGGFPYLDAVASSIRPIIWIGLVFSAMWLGIRVI
NFFANRYQDMQIENLGEEQFDKVRRRRTYVSIFRRVFVFVMVLGSIWIGLSEFANMEGFGKTLLTSAGIAGVVIGIAAQP
ILGNIIAGVQVAVTQPVRIGDSVIMDGNFSTVEDLRYTYAVLKTWDERRLIVPMRELITEMVENWSHTEVHQTCPVFLYI
DYGADIDAIRQQFISVVKDNKLWDNKTEPEIFVVEVTEKTIQLRGAVSAVGPVEAWTLACEIREQMLDYLYREQKHYLPA
EHLVLRQK
>Mature_648_residues
MQTPFGFLRTHSLQSHAQFLSRLLFIIGSLFILLNMACISAYAASSTEEIDSRNIEETVTTKPENLVEYAAQRIDKLKEE
GVTVSAVAAEVISPLEKRTVQQAGVLQGDSGLTGDYSENYYALDKLNAGLPPLSEPPNLSTPLATLEFFQSAVMKQQYDL
AAYALNMNLIDRESQSNQAMDLVKQLDFLLVEKQLYVFDKLPDRPDGLIEPPLGSTSSIQGIPRRSIQLGYIDYRERRVP
LHLERVRIDNGAPFWVFSAQTVANIDKLYEQYHPAKFERYLPSWTKLKLFNIAIWEFLALFIFFSFTMGIGWLLSTAAGK
LLNWYVMDKEGNRLGTLHHNGVEDLVNKLTVPLTFTISFSSVFTLVSGGFPYLDAVASSIRPIIWIGLVFSAMWLGIRVI
NFFANRYQDMQIENLGEEQFDKVRRRRTYVSIFRRVFVFVMVLGSIWIGLSEFANMEGFGKTLLTSAGIAGVVIGIAAQP
ILGNIIAGVQVAVTQPVRIGDSVIMDGNFSTVEDLRYTYAVLKTWDERRLIVPMRELITEMVENWSHTEVHQTCPVFLYI
DYGADIDAIRQQFISVVKDNKLWDNKTEPEIFVVEVTEKTIQLRGAVSAVGPVEAWTLACEIREQMLDYLYREQKHYLPA
EHLVLRQK

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=127, Percent_Identity=29.9212598425197, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 73472; Mature: 73472

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTPFGFLRTHSLQSHAQFLSRLLFIIGSLFILLNMACISAYAASSTEEIDSRNIEETVT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHC
TKPENLVEYAAQRIDKLKEEGVTVSAVAAEVISPLEKRTVQQAGVLQGDSGLTGDYSENY
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCE
YALDKLNAGLPPLSEPPNLSTPLATLEFFQSAVMKQQYDLAAYALNMNLIDRESQSNQAM
EEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECHHCCCCCCCHHHH
DLVKQLDFLLVEKQLYVFDKLPDRPDGLIEPPLGSTSSIQGIPRRSIQLGYIDYRERRVP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCC
LHLERVRIDNGAPFWVFSAQTVANIDKLYEQYHPAKFERYLPSWTKLKLFNIAIWEFLAL
EEEEEEEECCCCCEEEECHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHH
FIFFSFTMGIGWLLSTAAGKLLNWYVMDKEGNRLGTLHHNGVEDLVNKLTVPLTFTISFS
HHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEECCCCCCHHHHHHHHEECEEEEEEHH
SVFTLVSGGFPYLDAVASSIRPIIWIGLVFSAMWLGIRVINFFANRYQDMQIENLGEEQF
HHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHH
DKVRRRRTYVSIFRRVFVFVMVLGSIWIGLSEFANMEGFGKTLLTSAGIAGVVIGIAAQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCH
ILGNIIAGVQVAVTQPVRIGDSVIMDGNFSTVEDLRYTYAVLKTWDERRLIVPMRELITE
HHHHHHHCCEEEEECCEECCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
MVENWSHTEVHQTCPVFLYIDYGADIDAIRQQFISVVKDNKLWDNKTEPEIFVVEVTEKT
HHHCCCCCCHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHH
IQLRGAVSAVGPVEAWTLACEIREQMLDYLYREQKHYLPAEHLVLRQK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure
MQTPFGFLRTHSLQSHAQFLSRLLFIIGSLFILLNMACISAYAASSTEEIDSRNIEETVT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHC
TKPENLVEYAAQRIDKLKEEGVTVSAVAAEVISPLEKRTVQQAGVLQGDSGLTGDYSENY
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCE
YALDKLNAGLPPLSEPPNLSTPLATLEFFQSAVMKQQYDLAAYALNMNLIDRESQSNQAM
EEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECHHCCCCCCCHHHH
DLVKQLDFLLVEKQLYVFDKLPDRPDGLIEPPLGSTSSIQGIPRRSIQLGYIDYRERRVP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCC
LHLERVRIDNGAPFWVFSAQTVANIDKLYEQYHPAKFERYLPSWTKLKLFNIAIWEFLAL
EEEEEEEECCCCCEEEECHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHH
FIFFSFTMGIGWLLSTAAGKLLNWYVMDKEGNRLGTLHHNGVEDLVNKLTVPLTFTISFS
HHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEECCCCCCHHHHHHHHEECEEEEEEHH
SVFTLVSGGFPYLDAVASSIRPIIWIGLVFSAMWLGIRVINFFANRYQDMQIENLGEEQF
HHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHH
DKVRRRRTYVSIFRRVFVFVMVLGSIWIGLSEFANMEGFGKTLLTSAGIAGVVIGIAAQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCH
ILGNIIAGVQVAVTQPVRIGDSVIMDGNFSTVEDLRYTYAVLKTWDERRLIVPMRELITE
HHHHHHHCCEEEEECCEECCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
MVENWSHTEVHQTCPVFLYIDYGADIDAIRQQFISVVKDNKLWDNKTEPEIFVVEVTEKT
HHHCCCCCCHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHH
IQLRGAVSAVGPVEAWTLACEIREQMLDYLYREQKHYLPAEHLVLRQK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]