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The map label for this gene is dusA [H]

Identifier: 93005625

GI number: 93005625

Start: 936167

End: 937132

Strand: Reverse

Name: dusA [H]

Synonym: Pcryo_0795

Alternate gene names: 93005625

Gene position: 937132-936167 (Counterclockwise)

Preceding gene: 93005626

Following gene: 93005620

Centisome position: 30.63

GC content: 45.86

Gene sequence:

>966_bases
ATGGTTGATATGACCAATAAACGTCTTTCCGTTGCACCCATGATCGATTGGACAACGACGGATTATCGTTATTTTGCCCG
TCTTTTTAATCCGCATGTTTATTTGTACACCGAGATGATCAGTACGGGCGCGCTGATACATGGCAATCGTGCACGGCATT
TACGCTTTGATACTCTTGAACATCCGCTAGTACTGCAACTTGGCGGCGCAGATATCACTGAGATGACGCAGTGTGCAGAA
TTTGCTCAGCAGCATGGTTATGATGAGGTTAATATTAATGTGGGCTGCCCCTCTGATCGTGTACAGCACAATAAAATCGG
TGCTTGCTTGATGGCAGAACCCAATACCGTTGCAGACTTGGTCAAGCATATGCAAGCGGCAGTCGATATTCCAGTCACGG
TCAAGCATCGTATTGGCATTGATGACTTTGACAGTTATGAATTTATGGCGGATTTTGTACAAAAGGTAGCAGCAGGTGGT
TGTACACGTTTTATCGTTCATGCGCGTACGGCATGGCTACAAGGCTTGAGCCCAAAGCAAAATCGTGAGATACCGCCACT
GCGTTATGAAGATGTGTATCGTTTAAAACAGGACTTTCCTACCCTTGATATTGAGATTAATGGTGGGATTGAGACCGTCG
CTGATATTGAGGCACACCTTGAGCATATCGATGGCGTTATGATTGGGCGCGCCTTTTATCATAACCCGTATTTGTTAGCC
GAAGCCAATAGCTTGTGGGACACCCCTGTCCCGAAGCGTTCAGTTATACTAGCTCAGCTGTATCCTTATTTAGAAGAGCA
AATAGCTAAAGGTGAAGCACTTCCAACGATGACTCGGCACTATTTAGGGTTATTCCAAGGATTAGTGGGTGCTCGAAAAT
GGCGTCAAGCATTGAGCGGTAAGCCTCAATTGACCATCGATGATATCAAGCACGCGGCGACTGAAGTATTAGCCCTTAAC
CCTTAA

Upstream 100 bases:

>100_bases
TTTACAAGCCATTGCAAGTGATACGTAAACTGACACATATACCATCAGCATTTAGCATATACATCTTCAAAAATTTCTAA
AAAATTAAGGCTTAACCACT

Downstream 100 bases:

>100_bases
CCCTTAACCCTGATGCCTGATGCCTGATGCCTGATGAAACCTACTATTAAATAACAATGCATACGGCGTAAGTATTAATT
ATCAGATATTCACTGTTATT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MVDMTNKRLSVAPMIDWTTTDYRYFARLFNPHVYLYTEMISTGALIHGNRARHLRFDTLEHPLVLQLGGADITEMTQCAE
FAQQHGYDEVNINVGCPSDRVQHNKIGACLMAEPNTVADLVKHMQAAVDIPVTVKHRIGIDDFDSYEFMADFVQKVAAGG
CTRFIVHARTAWLQGLSPKQNREIPPLRYEDVYRLKQDFPTLDIEINGGIETVADIEAHLEHIDGVMIGRAFYHNPYLLA
EANSLWDTPVPKRSVILAQLYPYLEEQIAKGEALPTMTRHYLGLFQGLVGARKWRQALSGKPQLTIDDIKHAATEVLALN
P

Sequences:

>Translated_321_residues
MVDMTNKRLSVAPMIDWTTTDYRYFARLFNPHVYLYTEMISTGALIHGNRARHLRFDTLEHPLVLQLGGADITEMTQCAE
FAQQHGYDEVNINVGCPSDRVQHNKIGACLMAEPNTVADLVKHMQAAVDIPVTVKHRIGIDDFDSYEFMADFVQKVAAGG
CTRFIVHARTAWLQGLSPKQNREIPPLRYEDVYRLKQDFPTLDIEINGGIETVADIEAHLEHIDGVMIGRAFYHNPYLLA
EANSLWDTPVPKRSVILAQLYPYLEEQIAKGEALPTMTRHYLGLFQGLVGARKWRQALSGKPQLTIDDIKHAATEVLALN
P
>Mature_321_residues
MVDMTNKRLSVAPMIDWTTTDYRYFARLFNPHVYLYTEMISTGALIHGNRARHLRFDTLEHPLVLQLGGADITEMTQCAE
FAQQHGYDEVNINVGCPSDRVQHNKIGACLMAEPNTVADLVKHMQAAVDIPVTVKHRIGIDDFDSYEFMADFVQKVAAGG
CTRFIVHARTAWLQGLSPKQNREIPPLRYEDVYRLKQDFPTLDIEINGGIETVADIEAHLEHIDGVMIGRAFYHNPYLLA
EANSLWDTPVPKRSVILAQLYPYLEEQIAKGEALPTMTRHYLGLFQGLVGARKWRQALSGKPQLTIDDIKHAATEVLALN
P

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=249, Percent_Identity=26.9076305220884, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI145693211, Length=297, Percent_Identity=55.2188552188552, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1789660, Length=248, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=234, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323560, Length=284, Percent_Identity=21.830985915493, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24580595, Length=241, Percent_Identity=27.8008298755187, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19920448, Length=241, Percent_Identity=27.8008298755187, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19921524, Length=234, Percent_Identity=24.3589743589744, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36238; Mature: 36238

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDMTNKRLSVAPMIDWTTTDYRYFARLFNPHVYLYTEMISTGALIHGNRARHLRFDTLE
CCCCCCCCEEEEEEEECCCCHHHHHHHHHCCEEEEEEEHHHCCCEEECCCCCEEEECCCC
HPLVLQLGGADITEMTQCAEFAQQHGYDEVNINVGCPSDRVQHNKIGACLMAEPNTVADL
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHH
VKHMQAAVDIPVTVKHRIGIDDFDSYEFMADFVQKVAAGGCTRFIVHARTAWLQGLSPKQ
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
NREIPPLRYEDVYRLKQDFPTLDIEINGGIETVADIEAHLEHIDGVMIGRAFYHNPYLLA
CCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCEECHHHHCCCEEEE
EANSLWDTPVPKRSVILAQLYPYLEEQIAKGEALPTMTRHYLGLFQGLVGARKWRQALSG
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
KPQLTIDDIKHAATEVLALNP
CCCEEHHHHHHHHHHEEEECC
>Mature Secondary Structure
MVDMTNKRLSVAPMIDWTTTDYRYFARLFNPHVYLYTEMISTGALIHGNRARHLRFDTLE
CCCCCCCCEEEEEEEECCCCHHHHHHHHHCCEEEEEEEHHHCCCEEECCCCCEEEECCCC
HPLVLQLGGADITEMTQCAEFAQQHGYDEVNINVGCPSDRVQHNKIGACLMAEPNTVADL
CCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHH
VKHMQAAVDIPVTVKHRIGIDDFDSYEFMADFVQKVAAGGCTRFIVHARTAWLQGLSPKQ
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
NREIPPLRYEDVYRLKQDFPTLDIEINGGIETVADIEAHLEHIDGVMIGRAFYHNPYLLA
CCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCEECHHHHCCCEEEE
EANSLWDTPVPKRSVILAQLYPYLEEQIAKGEALPTMTRHYLGLFQGLVGARKWRQALSG
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
KPQLTIDDIKHAATEVLALNP
CCCEEHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]