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The map label for this gene is gacA [H]

Identifier: 93005507

GI number: 93005507

Start: 803841

End: 804476

Strand: Reverse

Name: gacA [H]

Synonym: Pcryo_0677

Alternate gene names: 93005507

Gene position: 804476-803841 (Counterclockwise)

Preceding gene: 93005510

Following gene: 93005506

Centisome position: 26.29

GC content: 42.45

Gene sequence:

>636_bases
ATGATTCGAGTATTAGTAGTAGATGATCATGATTTGGTGCGGATGGGTATTAGCCGTATGCTGGCAGATAGTGCAGATAT
CGAAGTCGTCGGTGAGGCAGACAGCGGTGATATGGCGATAAAGATGGCAAAACAGCTGCGTCCTGATGTGATATTACTGG
ATGTCAATATGCCCAATATCGGCGGTCTGGAAGCAACCAAACGCTTGATTCAGCTGGATATGGGTATCAAAATTTTGGCA
GTTAGTAGTATGTCAGCTCAACCTTATCCCTCAATGTTGATTAAAGCAGGCGTCAATGGCTATATTACTAAAGGCACACC
GCTTGATGAGATGATACGAGCGGTCAAAAAAATCTATCAAGGTGGACGCTACTTTAGCCAAGACGTCGCTGAACAATTAG
CTGACGTACTATTATCTGATAATGCCAATTCCCCTTTTGATTTGCTCAGTGATCGTGAGAAGCAAGTAGCAATGATGGTG
GTCAACTGTCAAAGCCCTCAACAAATTGCAGACCAGTTATTTGTCAGTGTGAAGACCATCAATACCTATCGTTATCGTAT
TTATGAAAAAGTAAATGTCGATAGTGATGTGAAATTGACGCATTTGGCGATTCGCCATGGTCTTATTCAACCCTAA

Upstream 100 bases:

>100_bases
TTTTATGCGTAAATGAGTGTTATTGGTCAAATAGATTGATTAAGATGACTTAAATTTAGCAGTGCACCCTTTATTCTGAT
AGATAGAAAAGAGTATAACA

Downstream 100 bases:

>100_bases
TTATCAAGTCCCTCTCATTAAGCACATTAGTTATTAATCCATGAATCCTGCCAAATCTGCCTCCAAGCTTATCGCTGTTA
TTACTGACTATCTAGAGCAA

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MIRVLVVDDHDLVRMGISRMLADSADIEVVGEADSGDMAIKMAKQLRPDVILLDVNMPNIGGLEATKRLIQLDMGIKILA
VSSMSAQPYPSMLIKAGVNGYITKGTPLDEMIRAVKKIYQGGRYFSQDVAEQLADVLLSDNANSPFDLLSDREKQVAMMV
VNCQSPQQIADQLFVSVKTINTYRYRIYEKVNVDSDVKLTHLAIRHGLIQP

Sequences:

>Translated_211_residues
MIRVLVVDDHDLVRMGISRMLADSADIEVVGEADSGDMAIKMAKQLRPDVILLDVNMPNIGGLEATKRLIQLDMGIKILA
VSSMSAQPYPSMLIKAGVNGYITKGTPLDEMIRAVKKIYQGGRYFSQDVAEQLADVLLSDNANSPFDLLSDREKQVAMMV
VNCQSPQQIADQLFVSVKTINTYRYRIYEKVNVDSDVKLTHLAIRHGLIQP
>Mature_211_residues
MIRVLVVDDHDLVRMGISRMLADSADIEVVGEADSGDMAIKMAKQLRPDVILLDVNMPNIGGLEATKRLIQLDMGIKILA
VSSMSAQPYPSMLIKAGVNGYITKGTPLDEMIRAVKKIYQGGRYFSQDVAEQLADVLLSDNANSPFDLLSDREKQVAMMV
VNCQSPQQIADQLFVSVKTINTYRYRIYEKVNVDSDVKLTHLAIRHGLIQP

Specific function: Forms part of a two-component regulatory system gacA/gacA(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=44.4976076555024, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=33, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786747, Length=206, Percent_Identity=29.126213592233, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788521, Length=207, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788712, Length=205, Percent_Identity=27.8048780487805, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082052, Length=64, Percent_Identity=46.875, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23408; Mature: 23408

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVLVVDDHDLVRMGISRMLADSADIEVVGEADSGDMAIKMAKQLRPDVILLDVNMPNI
CEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCC
GGLEATKRLIQLDMGIKILAVSSMSAQPYPSMLIKAGVNGYITKGTPLDEMIRAVKKIYQ
CCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHH
GGRYFSQDVAEQLADVLLSDNANSPFDLLSDREKQVAMMVVNCQSPQQIADQLFVSVKTI
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHEE
NTYRYRIYEKVNVDSDVKLTHLAIRHGLIQP
EEEEEEEEEEECCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MIRVLVVDDHDLVRMGISRMLADSADIEVVGEADSGDMAIKMAKQLRPDVILLDVNMPNI
CEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCC
GGLEATKRLIQLDMGIKILAVSSMSAQPYPSMLIKAGVNGYITKGTPLDEMIRAVKKIYQ
CCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHH
GGRYFSQDVAEQLADVLLSDNANSPFDLLSDREKQVAMMVVNCQSPQQIADQLFVSVKTI
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHEE
NTYRYRIYEKVNVDSDVKLTHLAIRHGLIQP
EEEEEEEEEEECCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002569 [H]