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The map label for this gene is pilS [H]

Identifier: 93005506

GI number: 93005506

Start: 802070

End: 803800

Strand: Reverse

Name: pilS [H]

Synonym: Pcryo_0676

Alternate gene names: 93005506

Gene position: 803800-802070 (Counterclockwise)

Preceding gene: 93005507

Following gene: 93005505

Centisome position: 26.27

GC content: 41.83

Gene sequence:

>1731_bases
ATGAATCCTGCCAAATCTGCCTCCAAGCTTATCGCTGTTATTACTGACTATCTAGAGCAAGATGATATTTTGCCTGTGCC
GCAGTTACGCAAACTGGGGATGATTTATAGCAGTTATCGCTTTATCGTCAGCTTGTTTTTTATGCTGATGGTATATATTA
CGGTTCGCACGGATGGCAGCCCATCATTGCCAAGTTTCTTGCAGCAAACCGTGCTCAGTTTTTACGTTTTGCTGAGTTTA
ATCTTGCTAGGATTATTTTATACCGTTGGTAAGCATATGCGCCGACAGCTGGCTTTTGGGTTGGCGCTAGATGTCATTAT
CCTAAGCTTACTGCTCTATACCGCTGGCGCACCTGATTTGCAGCTGACCATGCTATACATGGTGGTGGTAGCAGCAAGCT
TTATGTTATTACATGGCTCACAAGCATTAATTATTACTTTGCTTGCCATTATTTTTGTGATTTATCAGCAGTTTTTTTAT
GCCATTGCCAATAGTATGAGTTTGGCAAATTTAGGCAGTGCCCTACTCATGTCAGCCAGCTTTTTGGCAGTAGGCGGTCT
AAGCTGGTCGATATCGCAGCGCTTGGTACAGCTTGAAAAAGTGGCGGTTCGCCATGCCAAAGAAGTCGAGCGTTTAAATA
GTATCAATCAAGAAGTCATCTCTCAAATGGTCAATGGTGTCATCGTCATTGATGACAAACAAGTGGTCTTAGCCAACCTT
GCGGCTTATCAGCTACTTAGCATCCCAAGCCGTCATGACAATCAGTCAAAACCAGTAGCCGCTTTTGAGCAGCAACTTGG
CCAACAACATCTGCAATTGTTTAATGCTTGCTTATCGGTAGCCGCAGGACTAACGCGTACTTTTATTTATGAGCTGCCTG
CTGTTGCCAACTCTTCTATCATTGGCAAGCTACGTGTCGATATTATTCCGTTAAAAGACGACAGTAAACTCATTATATTA
GAGGATTTACGCCGTGAACAAGCCAGTGCGCAACAATTAAAGCTCGCCTCACTTGGACAGTTGACCGCCAGTATCGCTCA
TGAAATCAGAAACCCTTTGGCAGCTATTTCGCAAGCTAGCCAGTTGCTAATAGAAGATGTTATAGAAAGCGAGATGGACA
ATGACAATGCCCTAAGTCCTTTCGCTAATAATGATATAAATAGCCAGATGACTGGCAACCATGAGCTTTATAAAATGATA
TTTTCGCAAACAAAACGCGTTAATCGCATTATCGAAGACGTATTAAAACTGTCGCGCCAGCAAACGGCCAATCAACAAAC
CATTATCCTTGCTGATTGGATGCCTGTATTTTTAGACAATTACTTTAAAGGACATGATGTCTTTCTACATGTTCTTACCC
AGCCTACCATCTCTTTTGATATTCATCAGTTAGAGCAGGTACTCATCAATTTAATCAATAATGGTTTACGCTATAGTAGT
TACGCCCACCCTCATGCCTTTGTGGAAATCGAAATTTATTGTGCCGATAACGATGTTATAATCGATGTTTTGGATGGCGG
TACTGGTGTTAATGCAGATGATTTAAGCGCTTTATTTAATCCGTTTTTTACCACCGAACAATCAGGCACAGGGCTTGGGC
TATATTTATCACAAGCCTTTTGTGAAGCCAATCAGGCACGCTTACTGTATGTGCCTGAGCATCAAAAAACCTGCTTTAGG
CTAATTTTTCCTGCTGTTCATAATGACATCGTTAGTCCAACTTTTATCTAA

Upstream 100 bases:

>100_bases
GTCGATAGTGATGTGAAATTGACGCATTTGGCGATTCGCCATGGTCTTATTCAACCCTAATTATCAAGTCCCTCTCATTA
AGCACATTAGTTATTAATCC

Downstream 100 bases:

>100_bases
AGAAGAAAGAATTACTTCTCAAAAAAAATTGAAAGAAAATGTAAAACAAAAGGCTTTATATTGTTCATTAAAAAATACCT
GTAACCAATGCAGTTATTAA

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MNPAKSASKLIAVITDYLEQDDILPVPQLRKLGMIYSSYRFIVSLFFMLMVYITVRTDGSPSLPSFLQQTVLSFYVLLSL
ILLGLFYTVGKHMRRQLAFGLALDVIILSLLLYTAGAPDLQLTMLYMVVVAASFMLLHGSQALIITLLAIIFVIYQQFFY
AIANSMSLANLGSALLMSASFLAVGGLSWSISQRLVQLEKVAVRHAKEVERLNSINQEVISQMVNGVIVIDDKQVVLANL
AAYQLLSIPSRHDNQSKPVAAFEQQLGQQHLQLFNACLSVAAGLTRTFIYELPAVANSSIIGKLRVDIIPLKDDSKLIIL
EDLRREQASAQQLKLASLGQLTASIAHEIRNPLAAISQASQLLIEDVIESEMDNDNALSPFANNDINSQMTGNHELYKMI
FSQTKRVNRIIEDVLKLSRQQTANQQTIILADWMPVFLDNYFKGHDVFLHVLTQPTISFDIHQLEQVLINLINNGLRYSS
YAHPHAFVEIEIYCADNDVIIDVLDGGTGVNADDLSALFNPFFTTEQSGTGLGLYLSQAFCEANQARLLYVPEHQKTCFR
LIFPAVHNDIVSPTFI

Sequences:

>Translated_576_residues
MNPAKSASKLIAVITDYLEQDDILPVPQLRKLGMIYSSYRFIVSLFFMLMVYITVRTDGSPSLPSFLQQTVLSFYVLLSL
ILLGLFYTVGKHMRRQLAFGLALDVIILSLLLYTAGAPDLQLTMLYMVVVAASFMLLHGSQALIITLLAIIFVIYQQFFY
AIANSMSLANLGSALLMSASFLAVGGLSWSISQRLVQLEKVAVRHAKEVERLNSINQEVISQMVNGVIVIDDKQVVLANL
AAYQLLSIPSRHDNQSKPVAAFEQQLGQQHLQLFNACLSVAAGLTRTFIYELPAVANSSIIGKLRVDIIPLKDDSKLIIL
EDLRREQASAQQLKLASLGQLTASIAHEIRNPLAAISQASQLLIEDVIESEMDNDNALSPFANNDINSQMTGNHELYKMI
FSQTKRVNRIIEDVLKLSRQQTANQQTIILADWMPVFLDNYFKGHDVFLHVLTQPTISFDIHQLEQVLINLINNGLRYSS
YAHPHAFVEIEIYCADNDVIIDVLDGGTGVNADDLSALFNPFFTTEQSGTGLGLYLSQAFCEANQARLLYVPEHQKTCFR
LIFPAVHNDIVSPTFI
>Mature_576_residues
MNPAKSASKLIAVITDYLEQDDILPVPQLRKLGMIYSSYRFIVSLFFMLMVYITVRTDGSPSLPSFLQQTVLSFYVLLSL
ILLGLFYTVGKHMRRQLAFGLALDVIILSLLLYTAGAPDLQLTMLYMVVVAASFMLLHGSQALIITLLAIIFVIYQQFFY
AIANSMSLANLGSALLMSASFLAVGGLSWSISQRLVQLEKVAVRHAKEVERLNSINQEVISQMVNGVIVIDDKQVVLANL
AAYQLLSIPSRHDNQSKPVAAFEQQLGQQHLQLFNACLSVAAGLTRTFIYELPAVANSSIIGKLRVDIIPLKDDSKLIIL
EDLRREQASAQQLKLASLGQLTASIAHEIRNPLAAISQASQLLIEDVIESEMDNDNALSPFANNDINSQMTGNHELYKMI
FSQTKRVNRIIEDVLKLSRQQTANQQTIILADWMPVFLDNYFKGHDVFLHVLTQPTISFDIHQLEQVLINLINNGLRYSS
YAHPHAFVEIEIYCADNDVIIDVLDGGTGVNADDLSALFNPFFTTEQSGTGLGLYLSQAFCEANQARLLYVPEHQKTCFR
LIFPAVHNDIVSPTFI

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=257, Percent_Identity=27.6264591439689, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788549, Length=378, Percent_Identity=25.3968253968254, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1790346, Length=215, Percent_Identity=26.046511627907, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64132; Mature: 64132

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure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HCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]