Click here to switch to the map view.

The map label for this gene is pilR [H]

Identifier: 93005505

GI number: 93005505

Start: 800164

End: 801903

Strand: Reverse

Name: pilR [H]

Synonym: Pcryo_0675

Alternate gene names: 93005505

Gene position: 801903-800164 (Counterclockwise)

Preceding gene: 93005506

Following gene: 93005501

Centisome position: 26.21

GC content: 44.6

Gene sequence:

>1740_bases
ATGACAACCACCGCGCTAGTCGTTGATGACGAAGTAGATTTATGCCGTTTGATGCAAATCACCCTGACCAAAATGGGTAT
CAAAAGTGATGTTGCCTATACATTGTCGCAAGCGAAATCGTACTGGCAAGACAATGACTATGATTTTTGTCTAACCGATT
TAAAACTCCCTGATGGTTCGGGATTAGAGTTGGTCAAACATATCAGTGCCAGCTCCAGTAAGCCCATTGCGGTGATTACA
GCGCATGGTAGTATGGATTTAGCGATTGAAGCACTAAAGCTTGGGGCTTTTGATTTCGTCAATAAACCGCTTGAGCTACC
CCGCTTACGGCAATTGGTTGAAAACGCGCTAAAGGTAATACAACAAGATTTGACTGCCAAGTCCACGCCGCAACGCTCAC
CTGAACAGCAGATGCTTGATGAGCGCCTGATTGGTGATTCGGCGGTAATGCATCCTTTAAAAAACACGATTTTAAAATTG
GCTCGCTCACAAGCGCCAGTATTTTTGTCAGGGGCTTCAGGTACAGGTAAAGAAGTCGTTGCGCGGCTCATTCATGACTT
AAGCCCGCGGCGTGACGGCAGTTTTGTGCCAGTCAATTGCGGGGCGATACCATCAGAGCTGATGGAATCAGAGTTTTTTG
GTCATAAAAAAGGCAGCTTTACAGGTGCCGTTGCGGACAAGCAAGGTCTATTTCAGCAAGCCAATGGCGGTACTTTATTT
TTAGATGAGGTAGCAGACTTGCCTTTAGCGATGCAAGTGAAGTTGCTACGGGCTATTCAGGAGAAAACTGTACGCGCGAT
TGGCGATACCAAAGAAGTGCCTGTCGATATTCGTATCTTGTCAGCCACGCATAAAGATTTAAACCAGCTGGTACAGACAG
GGTTGTTCCGTCAGGATTTATATTACCGCATTAACGTGATTGAGCTTAAATTACCAACATTGAATGCACGGCGCGATGAT
ATACCGGTATTAGCCACGCATTTTTTAGCGCTGATTGCTGAAGAGTGGCAGTTGGAGTCGAAGCCTTTATTAACTGCAGA
GGCTTGCCTGCGTTTACAGCAGCATGATTTTGCGGGTAACGTTCGTGAGCTGCGTAATGTACTTGAACGTGCCGTTACTT
TGGCTGAAACCTCAACTATTGATATAAGCCATCTCGGCTTATCTGACTTAAATGTCCATCAACAGGCGCATATGCCTCAA
AAAATCAATGAAGATAACGCATCTGATTCAATAGAAAATATAGATAAAGACGATAGAGAACCATCATCTATCAAAAGTGT
CTCGACGCAGCAAGCAAAGGCTAATCTGCACCCTTATCGTACGATGACTCATCACTATGCTGCAGTGAAGCAATCTTCGA
TATCCGGTACGATATCCGACTCATCGTCTTCCGCAATAGGAATAGAGCAGCAATCAGTGACAGCAATGGCGGAGCGCGCT
GAATCAGTAAACAAGAACGCAAATAGTGATAAGACCGATTCTGTAGCCAATCTCGACAGTAGCATTGATGGCAATATTGA
TATATTAAACAGTATCGCGATAAAAAGCAGCGTCAGTGGTCAGCTACCAGCACAAGGGTTAGAGCACTATCTCCAAGAGC
AAGAAAGACAACTTATTATCACTGCACTTAAGCAAACTGGCTGGAATAAAACCCAAGCCGCTGAACTATTAGGAACCACT
TTTCGATCCTTACGCTATCGTATGACCAAACTTGAGATTGCAGAAGATCAAGCTGAATAA

Upstream 100 bases:

>100_bases
ATTAAAAAATACCTGTAACCAATGCAGTTATTAAGACTGATAAACCCTTAGAGCGTTATTTTATATACTTTTGTAGCACT
TGAAAACAATGAGATGATGT

Downstream 100 bases:

>100_bases
GGGGCTTTGATATAGAATTGTGACTAGATTAGAGCTTTTCCTTAGATGTTGGTTTTTCAATAACATTCTTTATAGCTGTA
TTATTTTTAATAATACTATT

Product: two component sigma-54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MTTTALVVDDEVDLCRLMQITLTKMGIKSDVAYTLSQAKSYWQDNDYDFCLTDLKLPDGSGLELVKHISASSSKPIAVIT
AHGSMDLAIEALKLGAFDFVNKPLELPRLRQLVENALKVIQQDLTAKSTPQRSPEQQMLDERLIGDSAVMHPLKNTILKL
ARSQAPVFLSGASGTGKEVVARLIHDLSPRRDGSFVPVNCGAIPSELMESEFFGHKKGSFTGAVADKQGLFQQANGGTLF
LDEVADLPLAMQVKLLRAIQEKTVRAIGDTKEVPVDIRILSATHKDLNQLVQTGLFRQDLYYRINVIELKLPTLNARRDD
IPVLATHFLALIAEEWQLESKPLLTAEACLRLQQHDFAGNVRELRNVLERAVTLAETSTIDISHLGLSDLNVHQQAHMPQ
KINEDNASDSIENIDKDDREPSSIKSVSTQQAKANLHPYRTMTHHYAAVKQSSISGTISDSSSSAIGIEQQSVTAMAERA
ESVNKNANSDKTDSVANLDSSIDGNIDILNSIAIKSSVSGQLPAQGLEHYLQEQERQLIITALKQTGWNKTQAAELLGTT
FRSLRYRMTKLEIAEDQAE

Sequences:

>Translated_579_residues
MTTTALVVDDEVDLCRLMQITLTKMGIKSDVAYTLSQAKSYWQDNDYDFCLTDLKLPDGSGLELVKHISASSSKPIAVIT
AHGSMDLAIEALKLGAFDFVNKPLELPRLRQLVENALKVIQQDLTAKSTPQRSPEQQMLDERLIGDSAVMHPLKNTILKL
ARSQAPVFLSGASGTGKEVVARLIHDLSPRRDGSFVPVNCGAIPSELMESEFFGHKKGSFTGAVADKQGLFQQANGGTLF
LDEVADLPLAMQVKLLRAIQEKTVRAIGDTKEVPVDIRILSATHKDLNQLVQTGLFRQDLYYRINVIELKLPTLNARRDD
IPVLATHFLALIAEEWQLESKPLLTAEACLRLQQHDFAGNVRELRNVLERAVTLAETSTIDISHLGLSDLNVHQQAHMPQ
KINEDNASDSIENIDKDDREPSSIKSVSTQQAKANLHPYRTMTHHYAAVKQSSISGTISDSSSSAIGIEQQSVTAMAERA
ESVNKNANSDKTDSVANLDSSIDGNIDILNSIAIKSSVSGQLPAQGLEHYLQEQERQLIITALKQTGWNKTQAAELLGTT
FRSLRYRMTKLEIAEDQAE
>Mature_578_residues
TTTALVVDDEVDLCRLMQITLTKMGIKSDVAYTLSQAKSYWQDNDYDFCLTDLKLPDGSGLELVKHISASSSKPIAVITA
HGSMDLAIEALKLGAFDFVNKPLELPRLRQLVENALKVIQQDLTAKSTPQRSPEQQMLDERLIGDSAVMHPLKNTILKLA
RSQAPVFLSGASGTGKEVVARLIHDLSPRRDGSFVPVNCGAIPSELMESEFFGHKKGSFTGAVADKQGLFQQANGGTLFL
DEVADLPLAMQVKLLRAIQEKTVRAIGDTKEVPVDIRILSATHKDLNQLVQTGLFRQDLYYRINVIELKLPTLNARRDDI
PVLATHFLALIAEEWQLESKPLLTAEACLRLQQHDFAGNVRELRNVLERAVTLAETSTIDISHLGLSDLNVHQQAHMPQK
INEDNASDSIENIDKDDREPSSIKSVSTQQAKANLHPYRTMTHHYAAVKQSSISGTISDSSSSAIGIEQQSVTAMAERAE
SVNKNANSDKTDSVANLDSSIDGNIDILNSIAIKSSVSGQLPAQGLEHYLQEQERQLIITALKQTGWNKTQAAELLGTTF
RSLRYRMTKLEIAEDQAE

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=370, Percent_Identity=41.6216216216216, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI1788550, Length=373, Percent_Identity=41.0187667560322, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1788905, Length=379, Percent_Identity=39.5778364116095, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1790299, Length=380, Percent_Identity=38.1578947368421, Blast_Score=263, Evalue=3e-71,
Organism=Escherichia coli, GI87082117, Length=276, Percent_Identity=46.0144927536232, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1789233, Length=241, Percent_Identity=46.8879668049792, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1789087, Length=243, Percent_Identity=43.2098765432099, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI87082152, Length=261, Percent_Identity=42.911877394636, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=38.8888888888889, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1787583, Length=215, Percent_Identity=43.2558139534884, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87081872, Length=236, Percent_Identity=38.9830508474576, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789828, Length=267, Percent_Identity=36.3295880149813, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI87081858, Length=321, Percent_Identity=27.7258566978193, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 63751; Mature: 63620

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTALVVDDEVDLCRLMQITLTKMGIKSDVAYTLSQAKSYWQDNDYDFCLTDLKLPDGS
CCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
GLELVKHISASSSKPIAVITAHGSMDLAIEALKLGAFDFVNKPLELPRLRQLVENALKVI
CHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHH
QQDLTAKSTPQRSPEQQMLDERLIGDSAVMHPLKNTILKLARSQAPVFLSGASGTGKEVV
HHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
ARLIHDLSPRRDGSFVPVNCGAIPSELMESEFFGHKKGSFTGAVADKQGLFQQANGGTLF
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEE
LDEVADLPLAMQVKLLRAIQEKTVRAIGDTKEVPVDIRILSATHKDLNQLVQTGLFRQDL
EHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC
YYRINVIELKLPTLNARRDDIPVLATHFLALIAEEWQLESKPLLTAEACLRLQQHDFAGN
EEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCC
VRELRNVLERAVTLAETSTIDISHLGLSDLNVHQQAHMPQKINEDNASDSIENIDKDDRE
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHCCCCCCC
PSSIKSVSTQQAKANLHPYRTMTHHYAAVKQSSISGTISDSSSSAIGIEQQSVTAMAERA
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCEECCCHHHHHHHHHHH
ESVNKNANSDKTDSVANLDSSIDGNIDILNSIAIKSSVSGQLPAQGLEHYLQEQERQLII
HHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
TALKQTGWNKTQAAELLGTTFRSLRYRMTKLEIAEDQAE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTTALVVDDEVDLCRLMQITLTKMGIKSDVAYTLSQAKSYWQDNDYDFCLTDLKLPDGS
CCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
GLELVKHISASSSKPIAVITAHGSMDLAIEALKLGAFDFVNKPLELPRLRQLVENALKVI
CHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHH
QQDLTAKSTPQRSPEQQMLDERLIGDSAVMHPLKNTILKLARSQAPVFLSGASGTGKEVV
HHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
ARLIHDLSPRRDGSFVPVNCGAIPSELMESEFFGHKKGSFTGAVADKQGLFQQANGGTLF
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEE
LDEVADLPLAMQVKLLRAIQEKTVRAIGDTKEVPVDIRILSATHKDLNQLVQTGLFRQDL
EHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC
YYRINVIELKLPTLNARRDDIPVLATHFLALIAEEWQLESKPLLTAEACLRLQQHDFAGN
EEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCC
VRELRNVLERAVTLAETSTIDISHLGLSDLNVHQQAHMPQKINEDNASDSIENIDKDDRE
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHCCCCCCC
PSSIKSVSTQQAKANLHPYRTMTHHYAAVKQSSISGTISDSSSSAIGIEQQSVTAMAERA
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCEECCCHHHHHHHHHHH
ESVNKNANSDKTDSVANLDSSIDGNIDILNSIAIKSSVSGQLPAQGLEHYLQEQERQLII
HHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
TALKQTGWNKTQAAELLGTTFRSLRYRMTKLEIAEDQAE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]