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The map label for this gene is nadB [H]

Identifier: 93005429

GI number: 93005429

Start: 713380

End: 715287

Strand: Reverse

Name: nadB [H]

Synonym: Pcryo_0599

Alternate gene names: 93005429

Gene position: 715287-713380 (Counterclockwise)

Preceding gene: 93005430

Following gene: 93005428

Centisome position: 23.38

GC content: 44.39

Gene sequence:

>1908_bases
ATGAGCGCGAACTGGTTAAATGGCAAAGATAACTGTAATGCACATCAGATCCTTGATGCTGTTGTGATAACAGATGTACT
TATCGTTGGGGCTGGGCTTGCAGGATTAAGCACCGCGCTTGCTTTACCGGAGTCGCTGAGCATTACCATATTAAGTAAAG
CTGCATTGGAAGTTTGCTCAAGCCATTACGCCCAAGGCGGTATCGCCGCAAGCTTGGACAAAAATGATTCGGTAGCTGAC
CATATTACCGATACGCTAATCGCTGGTGCAGGATTATGTGCAGCGGATAATACGACAAACATCCTCAGCGCTGGACAACA
AGCGGTTCAATGGCTATGTGAGCAAGGCGTACCCTTTACTCAGATTCCTCAAGCTAATAAAAAATACAATACTGAGCAAA
AAGTCTTCACTAAATTGCAAACTGCTGATTTACATCTAACCAAGGAAGGCGGTCATGGCTGTAGACGCGTTGCCCATGCT
GATGATGCAACGGGTCGCCATATTATGGAAGCCTTGACTATAAAAGCGCAAGCCGCTTCTAATATAACTATTTTGCCCTA
CCATGAAGCACTGTATTTATTAACTGACAACAGTGATAACCGATGCCAAGGGGCGCAAGTTTTTGATCATATTATTCAGC
GCCAAATCACATTTCATAGTCGCGCGGTTGTCTTAGCCAGTGGTGGTTTAGGGCAATTATTTCAACGAGCCAGCGCGCCC
AACGTCTGTGTCGGTGATGGCGTTATGATGGCGTGGCAAGCAGGCTGCCGTTTGGCAAATTTAGAATTCATCCAATTTCA
TCCGACTGGTCTAGCACTAGGAGATAGCAGCTTTTTGATTTCTGAAGCTTTACGCGGCGAAGGTGGGCGCTTATATTGTC
CACAAACTGGCGACCGCTTTATGCTAGAGATTGATGAGCGTGCAGAACTGGCGCCGCGAGATATTGTCGCTCGGGCAATA
GCTGAGCAGATTAATAATAACGGACTTGGCTACGTTCATCTTGACGTCAGTCACTTGCCAGCGGAATTTTTGCAAGAGCA
TTTCCCTCAGATTTATCAGACACTATTAGCACTTGGTATTGGTATAACTAAAGAGCCAATTCCGGTTGCCCCAACCGCCC
ATTATAGCTGTGGCGGCGTGGTCACTGATGCTAATGGCGTAACCGATGTAGCAGGTCTTTATGCCGCTGGTGAAGTTGCT
TATACAGGTCTGCATGGAGCAAACCGTTTGGCGAGCAATTCACTTTTGGAATGCGTAGTGGTTGGCCGCAATATCGCCGC
TGATTTACCTCGCTATTTAAAAGCGTTAGAGAATCTAGATACTGCTGCCATTTATGAGAGCATAAACTTATCAGCACCTA
CCATTTGGACAGCGCCAACACTCAAAGATACTCAAGCCATTACGCTCTCTTCTTTTATTACACATCCATTGACTTCAGAT
GATTACGAGGACACTGATAGCAATATAGATACTAATAATCAGGCTGGCAAAATAACTACAGAATTAAAAATTTTAATGAC
CACCTATATGGGTATCACTCGCACTGCCGAATCATTAGAGCAAATGTTAATACAGATACAGCAATGGCAACAGTGGCTTG
AAAAATCTGAGTTAAGTCAGCCCAATATAAATGCTTCAGACGACGCTATGACCATTGATAAACTTGCACACTTTCAATTA
GCAAGACAGTTACAATTAGCGACTTTAATTGTTCAGTCTGCTTGTCAGCGCCTTGAAAGTCGTGGCGGACACTATCGTAG
GGACTATCCCAACCTAGCAGCCACTGCTCGCATTAGTGTGATTGAGCCATTATCAAATCAAGCTATTTGCCCATTAAGTG
GTATCAATAATCCCACTTCAGAAATTCAGAAACATCATAAATTTGTCCAATCCGAACAAGCAGTATAG

Upstream 100 bases:

>100_bases
CGCATGCTTGATTTTGCTGAATCACAAAAACAGCGGGTAGCAGCAAGCGGTGATATCGTCAAAGACCGTGCATTGTTTGC
AAATGTTGGAGCAGCATAAT

Downstream 100 bases:

>100_bases
TGGCTTTATATTCAATCAATGCGGCACTGCTGGTCTAGCATATAGAGAAGCTCATAAAAAAAGCTTTGAGCAGCAGCAAT
TCAATGAATTTTGTCTGCTC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MSANWLNGKDNCNAHQILDAVVITDVLIVGAGLAGLSTALALPESLSITILSKAALEVCSSHYAQGGIAASLDKNDSVAD
HITDTLIAGAGLCAADNTTNILSAGQQAVQWLCEQGVPFTQIPQANKKYNTEQKVFTKLQTADLHLTKEGGHGCRRVAHA
DDATGRHIMEALTIKAQAASNITILPYHEALYLLTDNSDNRCQGAQVFDHIIQRQITFHSRAVVLASGGLGQLFQRASAP
NVCVGDGVMMAWQAGCRLANLEFIQFHPTGLALGDSSFLISEALRGEGGRLYCPQTGDRFMLEIDERAELAPRDIVARAI
AEQINNNGLGYVHLDVSHLPAEFLQEHFPQIYQTLLALGIGITKEPIPVAPTAHYSCGGVVTDANGVTDVAGLYAAGEVA
YTGLHGANRLASNSLLECVVVGRNIAADLPRYLKALENLDTAAIYESINLSAPTIWTAPTLKDTQAITLSSFITHPLTSD
DYEDTDSNIDTNNQAGKITTELKILMTTYMGITRTAESLEQMLIQIQQWQQWLEKSELSQPNINASDDAMTIDKLAHFQL
ARQLQLATLIVQSACQRLESRGGHYRRDYPNLAATARISVIEPLSNQAICPLSGINNPTSEIQKHHKFVQSEQAV

Sequences:

>Translated_635_residues
MSANWLNGKDNCNAHQILDAVVITDVLIVGAGLAGLSTALALPESLSITILSKAALEVCSSHYAQGGIAASLDKNDSVAD
HITDTLIAGAGLCAADNTTNILSAGQQAVQWLCEQGVPFTQIPQANKKYNTEQKVFTKLQTADLHLTKEGGHGCRRVAHA
DDATGRHIMEALTIKAQAASNITILPYHEALYLLTDNSDNRCQGAQVFDHIIQRQITFHSRAVVLASGGLGQLFQRASAP
NVCVGDGVMMAWQAGCRLANLEFIQFHPTGLALGDSSFLISEALRGEGGRLYCPQTGDRFMLEIDERAELAPRDIVARAI
AEQINNNGLGYVHLDVSHLPAEFLQEHFPQIYQTLLALGIGITKEPIPVAPTAHYSCGGVVTDANGVTDVAGLYAAGEVA
YTGLHGANRLASNSLLECVVVGRNIAADLPRYLKALENLDTAAIYESINLSAPTIWTAPTLKDTQAITLSSFITHPLTSD
DYEDTDSNIDTNNQAGKITTELKILMTTYMGITRTAESLEQMLIQIQQWQQWLEKSELSQPNINASDDAMTIDKLAHFQL
ARQLQLATLIVQSACQRLESRGGHYRRDYPNLAATARISVIEPLSNQAICPLSGINNPTSEIQKHHKFVQSEQAV
>Mature_634_residues
SANWLNGKDNCNAHQILDAVVITDVLIVGAGLAGLSTALALPESLSITILSKAALEVCSSHYAQGGIAASLDKNDSVADH
ITDTLIAGAGLCAADNTTNILSAGQQAVQWLCEQGVPFTQIPQANKKYNTEQKVFTKLQTADLHLTKEGGHGCRRVAHAD
DATGRHIMEALTIKAQAASNITILPYHEALYLLTDNSDNRCQGAQVFDHIIQRQITFHSRAVVLASGGLGQLFQRASAPN
VCVGDGVMMAWQAGCRLANLEFIQFHPTGLALGDSSFLISEALRGEGGRLYCPQTGDRFMLEIDERAELAPRDIVARAIA
EQINNNGLGYVHLDVSHLPAEFLQEHFPQIYQTLLALGIGITKEPIPVAPTAHYSCGGVVTDANGVTDVAGLYAAGEVAY
TGLHGANRLASNSLLECVVVGRNIAADLPRYLKALENLDTAAIYESINLSAPTIWTAPTLKDTQAITLSSFITHPLTSDD
YEDTDSNIDTNNQAGKITTELKILMTTYMGITRTAESLEQMLIQIQQWQQWLEKSELSQPNINASDDAMTIDKLAHFQLA
RQLQLATLIVQSACQRLESRGGHYRRDYPNLAATARISVIEPLSNQAICPLSGINNPTSEIQKHHKFVQSEQAV

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=550, Percent_Identity=30.3636363636364, Blast_Score=167, Evalue=4e-41,
Organism=Escherichia coli, GI1788928, Length=594, Percent_Identity=41.0774410774411, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1790597, Length=561, Percent_Identity=29.2335115864528, Blast_Score=201, Evalue=2e-52,
Organism=Escherichia coli, GI1786942, Length=566, Percent_Identity=28.9752650176678, Blast_Score=158, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17550100, Length=558, Percent_Identity=31.1827956989247, Blast_Score=192, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17505833, Length=382, Percent_Identity=35.3403141361257, Blast_Score=185, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6322701, Length=562, Percent_Identity=32.0284697508897, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6322416, Length=562, Percent_Identity=31.6725978647687, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322511, Length=489, Percent_Identity=25.1533742331288, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17137288, Length=579, Percent_Identity=31.9516407599309, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24655642, Length=579, Percent_Identity=31.9516407599309, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24655647, Length=579, Percent_Identity=31.9516407599309, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24663005, Length=577, Percent_Identity=28.7694974003466, Blast_Score=169, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 68619; Mature: 68488

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANWLNGKDNCNAHQILDAVVITDVLIVGAGLAGLSTALALPESLSITILSKAALEVCS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEHHHHHHHHHHH
SHYAQGGIAASLDKNDSVADHITDTLIAGAGLCAADNTTNILSAGQQAVQWLCEQGVPFT
HHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
QIPQANKKYNTEQKVFTKLQTADLHLTKEGGHGCRRVAHADDATGRHIMEALTIKAQAAS
CCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCC
NITILPYHEALYLLTDNSDNRCQGAQVFDHIIQRQITFHSRAVVLASGGLGQLFQRASAP
CCEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCC
NVCVGDGVMMAWQAGCRLANLEFIQFHPTGLALGDSSFLISEALRGEGGRLYCPQTGDRF
CEEECCCHHHHHHHCCEEECEEEEEEECCCEEECCCHHHHHHHHCCCCCEEECCCCCCEE
MLEIDERAELAPRDIVARAIAEQINNNGLGYVHLDVSHLPAEFLQEHFPQIYQTLLALGI
EEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHCC
GITKEPIPVAPTAHYSCGGVVTDANGVTDVAGLYAAGEVAYTGLHGANRLASNSLLECVV
CCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCHHHEECCCCHHHHHHHHHHHHHH
VGRNIAADLPRYLKALENLDTAAIYESINLSAPTIWTAPTLKDTQAITLSSFITHPLTSD
CCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCC
DYEDTDSNIDTNNQAGKITTELKILMTTYMGITRTAESLEQMLIQIQQWQQWLEKSELSQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PNINASDDAMTIDKLAHFQLARQLQLATLIVQSACQRLESRGGHYRRDYPNLAATARISV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEE
IEPLSNQAICPLSGINNPTSEIQKHHKFVQSEQAV
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SANWLNGKDNCNAHQILDAVVITDVLIVGAGLAGLSTALALPESLSITILSKAALEVCS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEHHHHHHHHHHH
SHYAQGGIAASLDKNDSVADHITDTLIAGAGLCAADNTTNILSAGQQAVQWLCEQGVPFT
HHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
QIPQANKKYNTEQKVFTKLQTADLHLTKEGGHGCRRVAHADDATGRHIMEALTIKAQAAS
CCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCC
NITILPYHEALYLLTDNSDNRCQGAQVFDHIIQRQITFHSRAVVLASGGLGQLFQRASAP
CCEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCC
NVCVGDGVMMAWQAGCRLANLEFIQFHPTGLALGDSSFLISEALRGEGGRLYCPQTGDRF
CEEECCCHHHHHHHCCEEECEEEEEEECCCEEECCCHHHHHHHHCCCCCEEECCCCCCEE
MLEIDERAELAPRDIVARAIAEQINNNGLGYVHLDVSHLPAEFLQEHFPQIYQTLLALGI
EEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHCC
GITKEPIPVAPTAHYSCGGVVTDANGVTDVAGLYAAGEVAYTGLHGANRLASNSLLECVV
CCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCHHHEECCCCHHHHHHHHHHHHHH
VGRNIAADLPRYLKALENLDTAAIYESINLSAPTIWTAPTLKDTQAITLSSFITHPLTSD
CCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCC
DYEDTDSNIDTNNQAGKITTELKILMTTYMGITRTAESLEQMLIQIQQWQQWLEKSELSQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PNINASDDAMTIDKLAHFQLARQLQLATLIVQSACQRLESRGGHYRRDYPNLAATARISV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEE
IEPLSNQAICPLSGINNPTSEIQKHHKFVQSEQAV
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]