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The map label for this gene is yjfH [C]

Identifier: 93005359

GI number: 93005359

Start: 625447

End: 626241

Strand: Reverse

Name: yjfH [C]

Synonym: Pcryo_0529

Alternate gene names: 93005359

Gene position: 626241-625447 (Counterclockwise)

Preceding gene: 93005360

Following gene: 93005351

Centisome position: 20.47

GC content: 44.91

Gene sequence:

>795_bases
ATGGTAGCAAATCCCACCGAGCTGATTACCTCAGATAAGAATACAACGGTCAAGCTAGTAAAAGCGTTATTGACGCAAGC
TCGCCAACGTAACAAACATGGACAAACTGTCATTGAAGGTATTCATCTTATTGATGCTGCCCTTCGTAGCGATTATCCAT
TTGTGCAAATATTGCTAGCAGAGTCCGCACACCATCATCCAGAGGTGCAGCAAGTTCTCACCCGCCTGCCCACTTATACG
CCTATCTTAACCTTATCGGATGCGCTTTATGAAAGTATTCGTAGCTTAGGTACTGGGATTGACATTATGGCAGTGATTAA
GATGCCAACCCCTAGCCTATCTATGATTCATGATGACTGCTTGATTCTCAATGACGTTCAAGATAGTGGTAATGTCGGCA
CATTACTGCGCACGGCAGCAGCTGTTGGAATCAAAAACATACTTTGCACCAGCGCTACTGCGCAAGCTTGGTCACCAAAA
ACATTACGGGCAGGAATGGGTGCCCAGTTTGCCCTAAATATATATGAGGGATTAAGTGTACAAGAGGTTTTAGACCATGT
GCAAACCCCTCTATTTGCCACCAGCTCGCACACTGATACCGTCATCTATCAGCATGATTTAAAAAAACCAATCGCTTGGA
TTATGGGACATGAAGGTCAAGGGGTTTGTAACGAGCTGATGCAGTGCGCAACCCCTATCGCTCTACCACAGCCCAATGGG
CAAGAAAGCCTCAATGTTGCGATTGCAGGCTCACTATGTTTGTATGAGACATTACGTCAAAGAAGTTATAACTAA

Upstream 100 bases:

>100_bases
AAGACATACAAGCACATTTTGACAGCACCTATAAAGCCATATCAATAATCATTTTATAGGCGCTGTCTATATTTTATACT
TTTTCTCTAAGCGATCTATT

Downstream 100 bases:

>100_bases
TATCATTTAACTGAATCAGCAAGACGATTTTTAAGTTGAATACAAAAAAACCCGCAATCAATGATAGCGGGTTTTTTATT
TTACTTTCACTAACAAGTAC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MVANPTELITSDKNTTVKLVKALLTQARQRNKHGQTVIEGIHLIDAALRSDYPFVQILLAESAHHHPEVQQVLTRLPTYT
PILTLSDALYESIRSLGTGIDIMAVIKMPTPSLSMIHDDCLILNDVQDSGNVGTLLRTAAAVGIKNILCTSATAQAWSPK
TLRAGMGAQFALNIYEGLSVQEVLDHVQTPLFATSSHTDTVIYQHDLKKPIAWIMGHEGQGVCNELMQCATPIALPQPNG
QESLNVAIAGSLCLYETLRQRSYN

Sequences:

>Translated_264_residues
MVANPTELITSDKNTTVKLVKALLTQARQRNKHGQTVIEGIHLIDAALRSDYPFVQILLAESAHHHPEVQQVLTRLPTYT
PILTLSDALYESIRSLGTGIDIMAVIKMPTPSLSMIHDDCLILNDVQDSGNVGTLLRTAAAVGIKNILCTSATAQAWSPK
TLRAGMGAQFALNIYEGLSVQEVLDHVQTPLFATSSHTDTVIYQHDLKKPIAWIMGHEGQGVCNELMQCATPIALPQPNG
QESLNVAIAGSLCLYETLRQRSYN
>Mature_264_residues
MVANPTELITSDKNTTVKLVKALLTQARQRNKHGQTVIEGIHLIDAALRSDYPFVQILLAESAHHHPEVQQVLTRLPTYT
PILTLSDALYESIRSLGTGIDIMAVIKMPTPSLSMIHDDCLILNDVQDSGNVGTLLRTAAAVGIKNILCTSATAQAWSPK
TLRAGMGAQFALNIYEGLSVQEVLDHVQTPLFATSSHTDTVIYQHDLKKPIAWIMGHEGQGVCNELMQCATPIALPQPNG
QESLNVAIAGSLCLYETLRQRSYN

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=297, Percent_Identity=26.2626262626263, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1790623, Length=152, Percent_Identity=27.6315789473684, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28773; Mature: 28773

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVANPTELITSDKNTTVKLVKALLTQARQRNKHGQTVIEGIHLIDAALRSDYPFVQILLA
CCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHH
ESAHHHPEVQQVLTRLPTYTPILTLSDALYESIRSLGTGIDIMAVIKMPTPSLSMIHDDC
HCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCE
LILNDVQDSGNVGTLLRTAAAVGIKNILCTSATAQAWSPKTLRAGMGAQFALNIYEGLSV
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEHHHHCCCH
QEVLDHVQTPLFATSSHTDTVIYQHDLKKPIAWIMGHEGQGVCNELMQCATPIALPQPNG
HHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCC
QESLNVAIAGSLCLYETLRQRSYN
CCCEEEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVANPTELITSDKNTTVKLVKALLTQARQRNKHGQTVIEGIHLIDAALRSDYPFVQILLA
CCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHH
ESAHHHPEVQQVLTRLPTYTPILTLSDALYESIRSLGTGIDIMAVIKMPTPSLSMIHDDC
HCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCE
LILNDVQDSGNVGTLLRTAAAVGIKNILCTSATAQAWSPKTLRAGMGAQFALNIYEGLSV
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEHHHHCCCH
QEVLDHVQTPLFATSSHTDTVIYQHDLKKPIAWIMGHEGQGVCNELMQCATPIALPQPNG
HHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCC
QESLNVAIAGSLCLYETLRQRSYN
CCCEEEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]