Click here to switch to the map view.

The map label for this gene is ipk

Identifier: 93005017

GI number: 93005017

Start: 217236

End: 218204

Strand: Reverse

Name: ipk

Synonym: Pcryo_0186

Alternate gene names: 93005017

Gene position: 218204-217236 (Counterclockwise)

Preceding gene: 93005018

Following gene: 93005016

Centisome position: 7.13

GC content: 43.03

Gene sequence:

>969_bases
ATGACTAAAAACGCGACTTCTGCATCGGTTATTACTCGCCTATCACCAGCAAAGATTAATTTATTTTTGCATATTACTGG
TAAGCGCGCTGATGGTTATCACAATTTGCAAACGGTGTTTCGCCTGCTCGATTGGGGCGATTATTTGCATTTTTCAGTAG
CAGATGAATTAATAATTACTATTGATAGCGTAGCAGACAATAGCGCTGTAGATAATAGTAGCCTGTGTGGTCAGCTGCTT
ACGCTAAGTGGTGCCGACACCATAACCAGCAGCATAGAGGACAATTTGATCTTTAAAGCGGCTAATGCCTTACTGGCATC
TGCCATAAACTCAAATTCGCTACCTAAGAATCTACCGAAAGTGTCAGTGAGGTTAGATAAGCACTTACCGATGGGCGCAG
GCTTAGGTGGTGGCTCATCCAATGCTGCGACGACATTATTAGTCTTAAATAAAATATGGCAGATTAATTTTGATCAAGAT
ACGCTTATAAAAATTGGCGCAAGCATTGGCGCTGATGTGCCGATATTTATTTTTGGACAAGATGCTATTGCGATGGGCAT
TGGCGAAGAGCTGACAGCGATTGATTTACCTGAGCAACAGTATTTAGTATTGACCCCTAAAGCTCACGTTAATACTGCCG
AGCTTTTTGCGCACTCCAAGCTACAGCGAGATATCGCTCCGCTATCAATTAAGACCATTCAAAACCATTCCAATGACTAT
GTGCAACATTTAAACACGCCTTATCAAAATGTCTTTACGCCAGTGGTCACCAATCTTGCTCCTGCCGTCGATGAAGCACT
ACGCTATTTGCAAGAGTTAGAGACTCAAGCACTGAGCACAGCAAGAATGACTGGCTCAGGTAGCGCAGTATTTTTGCCTT
TGGATGCAGGTGTGATATCTGATAAAGCGCGTTTGGCAAAGTGGATTGAAGAGGCGCCCTGTCCTGCCTATTTAGTTCGT
AATTTATGA

Upstream 100 bases:

>100_bases
AGTAATAGCACCAATAGATTTGATCGCTATTGCAAACAGCCATTTCTATAAATGGCTGTTTGCGTTTAAAAATTGCACTT
AAGAATTGTGACCATCATTT

Downstream 100 bases:

>100_bases
GTTAGATTTGAGCTATAGTGTGAAAATAAGAGGACTACAAATACAGCGTAAAAAGTTTTATGGATTCAATACCCGTTTTA
TTTCTGACCTATTTTTTAAT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTKNATSASVITRLSPAKINLFLHITGKRADGYHNLQTVFRLLDWGDYLHFSVADELIITIDSVADNSAVDNSSLCGQLL
TLSGADTITSSIEDNLIFKAANALLASAINSNSLPKNLPKVSVRLDKHLPMGAGLGGGSSNAATTLLVLNKIWQINFDQD
TLIKIGASIGADVPIFIFGQDAIAMGIGEELTAIDLPEQQYLVLTPKAHVNTAELFAHSKLQRDIAPLSIKTIQNHSNDY
VQHLNTPYQNVFTPVVTNLAPAVDEALRYLQELETQALSTARMTGSGSAVFLPLDAGVISDKARLAKWIEEAPCPAYLVR
NL

Sequences:

>Translated_322_residues
MTKNATSASVITRLSPAKINLFLHITGKRADGYHNLQTVFRLLDWGDYLHFSVADELIITIDSVADNSAVDNSSLCGQLL
TLSGADTITSSIEDNLIFKAANALLASAINSNSLPKNLPKVSVRLDKHLPMGAGLGGGSSNAATTLLVLNKIWQINFDQD
TLIKIGASIGADVPIFIFGQDAIAMGIGEELTAIDLPEQQYLVLTPKAHVNTAELFAHSKLQRDIAPLSIKTIQNHSNDY
VQHLNTPYQNVFTPVVTNLAPAVDEALRYLQELETQALSTARMTGSGSAVFLPLDAGVISDKARLAKWIEEAPCPAYLVR
NL
>Mature_321_residues
TKNATSASVITRLSPAKINLFLHITGKRADGYHNLQTVFRLLDWGDYLHFSVADELIITIDSVADNSAVDNSSLCGQLLT
LSGADTITSSIEDNLIFKAANALLASAINSNSLPKNLPKVSVRLDKHLPMGAGLGGGSSNAATTLLVLNKIWQINFDQDT
LIKIGASIGADVPIFIFGQDAIAMGIGEELTAIDLPEQQYLVLTPKAHVNTAELFAHSKLQRDIAPLSIKTIQNHSNDYV
QHLNTPYQNVFTPVVTNLAPAVDEALRYLQELETQALSTARMTGSGSAVFLPLDAGVISDKARLAKWIEEAPCPAYLVRN
L

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=299, Percent_Identity=37.4581939799331, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PSYCK (Q1QED3)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579454.1
- ProteinModelPortal:   Q1QED3
- SMR:   Q1QED3
- STRING:   Q1QED3
- GeneID:   4035666
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0186
- NMPDR:   fig|335284.3.peg.795
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   WIEEAPC
- PhylomeDB:   Q1QED3
- ProtClustDB:   PRK00343
- BioCyc:   PCRY335284:PCRYO_0186-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 34701; Mature: 34570

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: ACT_SITE 18-18 ACT_SITE 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNATSASVITRLSPAKINLFLHITGKRADGYHNLQTVFRLLDWGDYLHFSVADELIIT
CCCCCCCHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCEEEEE
IDSVADNSAVDNSSLCGQLLTLSGADTITSSIEDNLIFKAANALLASAINSNSLPKNLPK
EECCCCCCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VSVRLDKHLPMGAGLGGGSSNAATTLLVLNKIWQINFDQDTLIKIGASIGADVPIFIFGQ
EEEEEECCCCCCCCCCCCCCCHHHHHHEEHHHHEECCCCCHHHEECHHCCCCCEEEEECC
DAIAMGIGEELTAIDLPEQQYLVLTPKAHVNTAELFAHSKLQRDIAPLSIKTIQNHSNDY
CEEEECCCCCCEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEHHCCCHHH
VQHLNTPYQNVFTPVVTNLAPAVDEALRYLQELETQALSTARMTGSGSAVFLPLDAGVIS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCC
DKARLAKWIEEAPCPAYLVRNL
CHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure 
TKNATSASVITRLSPAKINLFLHITGKRADGYHNLQTVFRLLDWGDYLHFSVADELIIT
CCCCCCHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCEEEEE
IDSVADNSAVDNSSLCGQLLTLSGADTITSSIEDNLIFKAANALLASAINSNSLPKNLPK
EECCCCCCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VSVRLDKHLPMGAGLGGGSSNAATTLLVLNKIWQINFDQDTLIKIGASIGADVPIFIFGQ
EEEEEECCCCCCCCCCCCCCCHHHHHHEEHHHHEECCCCCHHHEECHHCCCCCEEEEECC
DAIAMGIGEELTAIDLPEQQYLVLTPKAHVNTAELFAHSKLQRDIAPLSIKTIQNHSNDY
CEEEECCCCCCEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEHHCCCHHH
VQHLNTPYQNVFTPVVTNLAPAVDEALRYLQELETQALSTARMTGSGSAVFLPLDAGVIS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCC
DKARLAKWIEEAPCPAYLVRNL
CHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA