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The map label for this gene is pilC [H]

Identifier: 93004899

GI number: 93004899

Start: 76476

End: 77699

Strand: Reverse

Name: pilC [H]

Synonym: Pcryo_0068

Alternate gene names: 93004899

Gene position: 77699-76476 (Counterclockwise)

Preceding gene: 93004900

Following gene: 93004898

Centisome position: 2.54

GC content: 42.4

Gene sequence:

>1224_bases
ATGGCAAAAGCTAAGACCGACATGCTGTTAGACTTTGTCTATGATGGGGTAAATCGGCGCGGGCAAAAGGTAAAAGGTGA
GACGACCAGTCGTAGCTTAGAGCTTGCCAAAGCCACGTTGCGCAAACAAGGTATTACGGTTAAAGGTATCAAGAAAAAGC
CAAAACCCTTATACACCTTTAAGAAGTCCATCAAGCCTATCGATATTGCTATTTTTGCACGTCAATTGGCAACTATGATG
AAAGCTGGGGTGCCATTGACTCAATCCTTTGAGATTGTCGCGGACTCTCTAGATAATCCGAGTATGAAAGACTTGGTATT
ACAAATTAAAGCCGATATTGAAGCTGGTGGTACTTTTGCATCGGCATTACGTCGCCATCCCCGCTATTTTGACGATCTTT
TTTGCTCGCTTGTTGATTCTGGCGAGCAGTCAGGTGCGTTAGAAACCATGCTAGAGCGAGTTGCGACTTATAAAGAGAAA
AGCGAGCTGTTAAAAGCTAAAATTAAAAAAGCTATGAAATATCCTATCGCGGTCGTTGTAGTTGCTATTATTGTCACCAT
TATTCTGCTTGTTAAAGTCGTTCCAGTGTTTGCTGATTTATTTAGCTCTTTTGGTGCAGAATTACCTGCTTTTACCCAAA
TGGTCGTAGGTATGTCTGAGTGGATGCAAGCATGGTGGTTCATCTTAATTGTATTTATTGGTGGCGCAATTATCGGCTTT
TCAGAAGCTAAGAAGCGTTCAAAGAAATTTCGTGATGTCTTAGACCGTGCGGTATTAAAAGCCCCAATATTTGGCAATAT
CGCTTACCAAGCGGTGATTGCGCGTTTTGCTCGTACGCTGTCTACAACTTTTGCTGCCGGCGTACCGCTTATTGATGCGC
TCGATTCAACTGCTGGCGCGGCCAATAATGTAGTATTTTATAACGCTATTCAGCAAATCAAAAATGACGTCTCTACGGGA
CAACAATTACAGTTTTCCATACGTTCAACCAATTTATTTCCAAGCCTTGCTATTCAAATGGTAGGTATCGGTGAAGAGTC
TGGTAGCTTAGAAGAGATGCTCGATAAAGTAGCAGTTTATTATGAAAACGAAGTTGATAACGCTGTTGATGGTTTGACCA
GCTTGATGGAGCCGCTTATTATGGCGGTACTTGGCGTATTAGTTGGTGGTTTGGTCATTGCCATGTACTTACCAATCTTC
CAGATGGGCTCAGTAGTAGGTTGA

Upstream 100 bases:

>100_bases
TAAAATAAAGTATATCCGCTTAAAACAATGGGTTAACTACGTTTTATAGCAGGCATTCTCTATATTGGACTATATCAGAC
CATTTTTTGAAGGAAGGGAC

Downstream 100 bases:

>100_bases
AAGCTAATTAAAGGACAGCTAATTCATGCAATTTATACAGTTACTACAAGAAAATATGACCATTGCTTTGGTCGTATTTG
GTCTACTAGGGCTGTGCGTC

Product: type II secretion system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MAKAKTDMLLDFVYDGVNRRGQKVKGETTSRSLELAKATLRKQGITVKGIKKKPKPLYTFKKSIKPIDIAIFARQLATMM
KAGVPLTQSFEIVADSLDNPSMKDLVLQIKADIEAGGTFASALRRHPRYFDDLFCSLVDSGEQSGALETMLERVATYKEK
SELLKAKIKKAMKYPIAVVVVAIIVTIILLVKVVPVFADLFSSFGAELPAFTQMVVGMSEWMQAWWFILIVFIGGAIIGF
SEAKKRSKKFRDVLDRAVLKAPIFGNIAYQAVIARFARTLSTTFAAGVPLIDALDSTAGAANNVVFYNAIQQIKNDVSTG
QQLQFSIRSTNLFPSLAIQMVGIGEESGSLEEMLDKVAVYYENEVDNAVDGLTSLMEPLIMAVLGVLVGGLVIAMYLPIF
QMGSVVG

Sequences:

>Translated_407_residues
MAKAKTDMLLDFVYDGVNRRGQKVKGETTSRSLELAKATLRKQGITVKGIKKKPKPLYTFKKSIKPIDIAIFARQLATMM
KAGVPLTQSFEIVADSLDNPSMKDLVLQIKADIEAGGTFASALRRHPRYFDDLFCSLVDSGEQSGALETMLERVATYKEK
SELLKAKIKKAMKYPIAVVVVAIIVTIILLVKVVPVFADLFSSFGAELPAFTQMVVGMSEWMQAWWFILIVFIGGAIIGF
SEAKKRSKKFRDVLDRAVLKAPIFGNIAYQAVIARFARTLSTTFAAGVPLIDALDSTAGAANNVVFYNAIQQIKNDVSTG
QQLQFSIRSTNLFPSLAIQMVGIGEESGSLEEMLDKVAVYYENEVDNAVDGLTSLMEPLIMAVLGVLVGGLVIAMYLPIF
QMGSVVG
>Mature_406_residues
AKAKTDMLLDFVYDGVNRRGQKVKGETTSRSLELAKATLRKQGITVKGIKKKPKPLYTFKKSIKPIDIAIFARQLATMMK
AGVPLTQSFEIVADSLDNPSMKDLVLQIKADIEAGGTFASALRRHPRYFDDLFCSLVDSGEQSGALETMLERVATYKEKS
ELLKAKIKKAMKYPIAVVVVAIIVTIILLVKVVPVFADLFSSFGAELPAFTQMVVGMSEWMQAWWFILIVFIGGAIIGFS
EAKKRSKKFRDVLDRAVLKAPIFGNIAYQAVIARFARTLSTTFAAGVPLIDALDSTAGAANNVVFYNAIQQIKNDVSTGQ
QLQFSIRSTNLFPSLAIQMVGIGEESGSLEEMLDKVAVYYENEVDNAVDGLTSLMEPLIMAVLGVLVGGLVIAMYLPIFQ
MGSVVG

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=402, Percent_Identity=28.6069651741293, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786295, Length=394, Percent_Identity=27.6649746192893, Blast_Score=164, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44490; Mature: 44359

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKAKTDMLLDFVYDGVNRRGQKVKGETTSRSLELAKATLRKQGITVKGIKKKPKPLYTF
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]