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The map label for this gene is pilB [H]

Identifier: 93004900

GI number: 93004900

Start: 77937

End: 79649

Strand: Reverse

Name: pilB [H]

Synonym: Pcryo_0069

Alternate gene names: 93004900

Gene position: 79649-77937 (Counterclockwise)

Preceding gene: 93004912

Following gene: 93004899

Centisome position: 2.6

GC content: 44.13

Gene sequence:

>1713_bases
ATGTCTATCACAACCAGTAAATACGGTGGCTTAGCACAGCAGCTGATTAGCGAAGGTATCGTAAGCGAAGCGCATATGAA
AGCGGCGCAGACTGATTCGCAGCAGCAGCAAGTAGGACTGGTCTCTTATCTGGTAGAGCATAAGCTTGCCAATGCCTATC
AATTGGCTCAAATGCTATCGCAAGCATTCGGCGATCCTTTATTTGATCTTGATACGCTCAGCTCAGAGGTCATCCCAAAA
GACTTGGTCGACGAAAAAATCGTCCGTAAGTTTAATGCCTTGCCAATGTTTAAGCGTGGGCAACGCTTATTTGTGGCACT
GAGTGATCCGACCCGTATCGATGCGATTGATGCGATTGCCTTTAACTCTCGCTTGTCAATTGAAACCGTTGTAGTCGAAG
AAGATAAGCTTAAAAGGCGTATTGAAAGCTTCTATGCTGATAGCATGCAAAATTTTGATAGCTTTTCTAATAGTGATTTG
CATATTGGCTTTGATGAGAGTCAAGAAGAAGATGACGGCGAGACCAAAATCTCTGATAGCGTCGAGGAAGCACCGGTCGT
CAAGTTTGTTAATAAAATGCTAGTAGATGCCATTCGTATGGGTGCGTCCGACTTACATTTTGAGCCCTATGAAAAAAGCT
ATCGAGTGCGCTTTCGAGTCGACGGTGTGATGCAAAAAATGGCCAACCCGCCCGTACAACTCGCGGGGAAAATTGCTGCG
CGTTTAAAAGTAATGTCACAAATGGATATCTCAGAGCGCCGTGTCCCTCAAGATGGACGTATTAAGCTAAAGATATCTAA
AAATAAGGCCATCGATTTTCGGGTAAGTTGCCTACCTACCTTGTTTGGCGAAAAACTGGTTTTGCGTATTCTAGATCCCT
CATCAGCCATGCTTGGTATTGAAGCGTTGGGCTATGAGCCTGACCAACAAGATATGTTTTTGGAGGCGTTACATAAACCG
CAAGGCATGCTCCTAATTACTGGTCCCACTGGTTCTGGTAAAACCGTCTCACTCTATACTGGTATTAACATCCTGAATAC
TGGTTCGACCAATATCTCGACTGCCGAAGATCCTGTCGAAATTAACCTTGAAGGTATTAATCAGGTCAACGTCAACCCAA
AAGTGGGGCTGACTTTCTCCACCGCCCTTAAGTCTTTCTTACGCCAAGATCCTGATATTGTGATGGTTGGTGAGATTCGT
GATCTTGAAACTGCTGAGATTGCGATTAAAGCAGCACAGACAGGGCACATGGTACTTTCAACCTTACATACCAATTCAGC
GCCTGAAACGTTAACGCGATTACGCAATATGGGTGTCGCTTCTTTTAATATTGCCACTTCCGTGAACTTGGTCATTGCTC
AGCGATTGGCACGGCGCTTATGTAAAAACTGTAAAAAACCCATTAGTATCCCACGTCAAAGTCTGCTTGAGCTTGGTTTT
ACAGATGCAGATTTGGACAATACAGACAATATCATCTACGAGCCAGTCGGTTGTAATGAGTGCCGTGAAGGATACAAAGG
ACGTGTTGGTATTTATGAGGTGATGAAAGTCACTCCAGATATTTCACGTATTATCATGGAAGATGGTAACGCGATAGATA
TCAAAGATGCGGCGCTAAAAAACGGTTTTAGAGACTTACGTCGCTCTGGTGTTTTAAAGGTTTTACAAGGCGTCACCAGT
ATTCAAGAAATGATGCGCGTGACTTCTGAGTAG

Upstream 100 bases:

>100_bases
CAACTATCAACGATAAAATGGCAATAAGTAAAACATGGTGAATAGCCTGCTATTTAGGTAAATATATCGCCTACCAGATA
TCGAGCAAGGAGAAGTCTTA

Downstream 100 bases:

>100_bases
CATATTTGGTTGAAAAGATTGGTAAAGATATTGCTTTGAGTAAATTTTATTATTTCCGTACAGCTTGTTGTTCAATAACT
ATTAAACAATAATAAGTAGT

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MSITTSKYGGLAQQLISEGIVSEAHMKAAQTDSQQQQVGLVSYLVEHKLANAYQLAQMLSQAFGDPLFDLDTLSSEVIPK
DLVDEKIVRKFNALPMFKRGQRLFVALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKRRIESFYADSMQNFDSFSNSDL
HIGFDESQEEDDGETKISDSVEEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMANPPVQLAGKIAA
RLKVMSQMDISERRVPQDGRIKLKISKNKAIDFRVSCLPTLFGEKLVLRILDPSSAMLGIEALGYEPDQQDMFLEALHKP
QGMLLITGPTGSGKTVSLYTGINILNTGSTNISTAEDPVEINLEGINQVNVNPKVGLTFSTALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPISIPRQSLLELGF
TDADLDNTDNIIYEPVGCNECREGYKGRVGIYEVMKVTPDISRIIMEDGNAIDIKDAALKNGFRDLRRSGVLKVLQGVTS
IQEMMRVTSE

Sequences:

>Translated_570_residues
MSITTSKYGGLAQQLISEGIVSEAHMKAAQTDSQQQQVGLVSYLVEHKLANAYQLAQMLSQAFGDPLFDLDTLSSEVIPK
DLVDEKIVRKFNALPMFKRGQRLFVALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKRRIESFYADSMQNFDSFSNSDL
HIGFDESQEEDDGETKISDSVEEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMANPPVQLAGKIAA
RLKVMSQMDISERRVPQDGRIKLKISKNKAIDFRVSCLPTLFGEKLVLRILDPSSAMLGIEALGYEPDQQDMFLEALHKP
QGMLLITGPTGSGKTVSLYTGINILNTGSTNISTAEDPVEINLEGINQVNVNPKVGLTFSTALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPISIPRQSLLELGF
TDADLDNTDNIIYEPVGCNECREGYKGRVGIYEVMKVTPDISRIIMEDGNAIDIKDAALKNGFRDLRRSGVLKVLQGVTS
IQEMMRVTSE
>Mature_569_residues
SITTSKYGGLAQQLISEGIVSEAHMKAAQTDSQQQQVGLVSYLVEHKLANAYQLAQMLSQAFGDPLFDLDTLSSEVIPKD
LVDEKIVRKFNALPMFKRGQRLFVALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKRRIESFYADSMQNFDSFSNSDLH
IGFDESQEEDDGETKISDSVEEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMANPPVQLAGKIAAR
LKVMSQMDISERRVPQDGRIKLKISKNKAIDFRVSCLPTLFGEKLVLRILDPSSAMLGIEALGYEPDQQDMFLEALHKPQ
GMLLITGPTGSGKTVSLYTGINILNTGSTNISTAEDPVEINLEGINQVNVNPKVGLTFSTALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPISIPRQSLLELGFT
DADLDNTDNIIYEPVGCNECREGYKGRVGIYEVMKVTPDISRIIMEDGNAIDIKDAALKNGFRDLRRSGVLKVLQGVTSI
QEMMRVTSE

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=395, Percent_Identity=48.3544303797468, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1786296, Length=475, Percent_Identity=39.1578947368421, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI87082188, Length=277, Percent_Identity=30.6859205776173, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 63161; Mature: 63030

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITTSKYGGLAQQLISEGIVSEAHMKAAQTDSQQQQVGLVSYLVEHKLANAYQLAQMLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAFGDPLFDLDTLSSEVIPKDLVDEKIVRKFNALPMFKRGQRLFVALSDPTRIDAIDAIA
HHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHEE
FNSRLSIETVVVEEDKLKRRIESFYADSMQNFDSFSNSDLHIGFDESQEEDDGETKISDS
ECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHCC
VEEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMANPPVQLAGKIAA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHH
RLKVMSQMDISERRVPQDGRIKLKISKNKAIDFRVSCLPTLFGEKLVLRILDPSSAMLGI
HHHHHHHCCHHHHCCCCCCCEEEEEECCCCEEEEHHHHHHHHCCEEEEEEECCCCHHEEH
EALGYEPDQQDMFLEALHKPQGMLLITGPTGSGKTVSLYTGINILNTGSTNISTAEDPVE
HHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEEECCCCCCCCCCCCEE
INLEGINQVNVNPKVGLTFSTALKSFLRQDPDIVMVGEIRDLETAEIAIKAAQTGHMVLS
EEEECCEEECCCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCHHHHH
TLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPISIPRQSLLELGF
HHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
TDADLDNTDNIIYEPVGCNECREGYKGRVGIYEVMKVTPDISRIIMEDGNAIDIKDAALK
CCCCCCCCCCEEECCCCCHHHHHCCCCCCCHHHHHHCCCCHHHHHHCCCCEEECHHHHHH
NGFRDLRRSGVLKVLQGVTSIQEMMRVTSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SITTSKYGGLAQQLISEGIVSEAHMKAAQTDSQQQQVGLVSYLVEHKLANAYQLAQMLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAFGDPLFDLDTLSSEVIPKDLVDEKIVRKFNALPMFKRGQRLFVALSDPTRIDAIDAIA
HHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHEE
FNSRLSIETVVVEEDKLKRRIESFYADSMQNFDSFSNSDLHIGFDESQEEDDGETKISDS
ECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHCC
VEEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMANPPVQLAGKIAA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHH
RLKVMSQMDISERRVPQDGRIKLKISKNKAIDFRVSCLPTLFGEKLVLRILDPSSAMLGI
HHHHHHHCCHHHHCCCCCCCEEEEEECCCCEEEEHHHHHHHHCCEEEEEEECCCCHHEEH
EALGYEPDQQDMFLEALHKPQGMLLITGPTGSGKTVSLYTGINILNTGSTNISTAEDPVE
HHHCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEEECCCCCCCCCCCCEE
INLEGINQVNVNPKVGLTFSTALKSFLRQDPDIVMVGEIRDLETAEIAIKAAQTGHMVLS
EEEECCEEECCCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCHHHHH
TLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPISIPRQSLLELGF
HHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
TDADLDNTDNIIYEPVGCNECREGYKGRVGIYEVMKVTPDISRIIMEDGNAIDIKDAALK
CCCCCCCCCCEEECCCCCHHHHHCCCCCCCHHHHHHCCCCHHHHHHCCCCEEECHHHHHH
NGFRDLRRSGVLKVLQGVTSIQEMMRVTSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]