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The map label for this gene is pilD [H]

Identifier: 93004898

GI number: 93004898

Start: 75551

End: 76450

Strand: Reverse

Name: pilD [H]

Synonym: Pcryo_0067

Alternate gene names: 93004898

Gene position: 76450-75551 (Counterclockwise)

Preceding gene: 93004899

Following gene: 93004897

Centisome position: 2.5

GC content: 42.44

Gene sequence:

>900_bases
ATGCAATTTATACAGTTACTACAAGAAAATATGACCATTGCTTTGGTCGTATTTGGTCTACTAGGGCTGTGCGTCGGTAG
CTTCTTAAACGTGGTAATTCATCGCACTCCATTGATAATGGTATCGGCTTGGCGAAAAGAGTGTAGCCAGTTTATGTATG
AGCAAGCAGATATGCCGCGTGAGCATACGATGCCACTAGTTAATATTGTTGCCACTGATACACCTATTACCTTAAGTAAA
CCAGCATCGCGCTGCCCGCACTGTGCCCACAAAATTAAGTGGTATGAAAACATACCTTTAATTAGCTGGATAGTACTACG
CGGACGTTGCTCAGAGTGCAAAGCGGCTATCGGGCTTCGATATCCTATCGTTGAGCTGGTCACTGCTCTGCTATCTGCAC
TAGTCATTTATCATTTTGGCGTTAATATGGTAGGGCTGTCGGCATTAGTTTTAGTCTGGACACTCATCGCCTTAACTGGT
ATCGATTTTGATACTCAGTTATTGCCTGACCGCTTAACTTTTCCATTAGCAGGGCTAGGATTGGCGGTCAATTCACAAGG
TTGGTTTGTGCCCCCTACTCAGTCGATATGGGGCTTGTTATTGGGATTTTTATCGTTATGGATAGTGGTCAAGATATTTT
ATTTAATCACCAAAAAGCATGGCATGGGACAAGGCGACTTTAAATTATTGGCAGTACTGGGTGCGTGGCTTGGACCTATG
ATGTTACCCTTGATTATTTTATTATCATCATTGCTCGGCTCTATCGTTGGGCTTATCTTGATGAAAAAACAAGGCGAGAG
CAAGCCTTTTGCCTTTGGTCCTTATATTGCCATTGCTGGTATTGTGGCATTGTTATACGGCTCAGATATCGTTAATTGGT
ATTTAGGTATGTATACTTAA

Upstream 100 bases:

>100_bases
CTTGGCGTATTAGTTGGTGGTTTGGTCATTGCCATGTACTTACCAATCTTCCAGATGGGCTCAGTAGTAGGTTGAAAGCT
AATTAAAGGACAGCTAATTC

Downstream 100 bases:

>100_bases
GCAGTAGCCATTTCCCAATAGTTATGTTTAACCTTTCATTGATTACAGTCATTATCCTTCTATCAGTCAGATAAGTCATC
ATTATGTCAAAATACGCAGC

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MQFIQLLQENMTIALVVFGLLGLCVGSFLNVVIHRTPLIMVSAWRKECSQFMYEQADMPREHTMPLVNIVATDTPITLSK
PASRCPHCAHKIKWYENIPLISWIVLRGRCSECKAAIGLRYPIVELVTALLSALVIYHFGVNMVGLSALVLVWTLIALTG
IDFDTQLLPDRLTFPLAGLGLAVNSQGWFVPPTQSIWGLLLGFLSLWIVVKIFYLITKKHGMGQGDFKLLAVLGAWLGPM
MLPLIILLSSLLGSIVGLILMKKQGESKPFAFGPYIAIAGIVALLYGSDIVNWYLGMYT

Sequences:

>Translated_299_residues
MQFIQLLQENMTIALVVFGLLGLCVGSFLNVVIHRTPLIMVSAWRKECSQFMYEQADMPREHTMPLVNIVATDTPITLSK
PASRCPHCAHKIKWYENIPLISWIVLRGRCSECKAAIGLRYPIVELVTALLSALVIYHFGVNMVGLSALVLVWTLIALTG
IDFDTQLLPDRLTFPLAGLGLAVNSQGWFVPPTQSIWGLLLGFLSLWIVVKIFYLITKKHGMGQGDFKLLAVLGAWLGPM
MLPLIILLSSLLGSIVGLILMKKQGESKPFAFGPYIAIAGIVALLYGSDIVNWYLGMYT
>Mature_299_residues
MQFIQLLQENMTIALVVFGLLGLCVGSFLNVVIHRTPLIMVSAWRKECSQFMYEQADMPREHTMPLVNIVATDTPITLSK
PASRCPHCAHKIKWYENIPLISWIVLRGRCSECKAAIGLRYPIVELVTALLSALVIYHFGVNMVGLSALVLVWTLIALTG
IDFDTQLLPDRLTFPLAGLGLAVNSQGWFVPPTQSIWGLLLGFLSLWIVVKIFYLITKKHGMGQGDFKLLAVLGAWLGPM
MLPLIILLSSLLGSIVGLILMKKQGESKPFAFGPYIAIAGIVALLYGSDIVNWYLGMYT

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=281, Percent_Identity=35.2313167259786, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1789732, Length=135, Percent_Identity=41.4814814814815, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 33075; Mature: 33075

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFIQLLQENMTIALVVFGLLGLCVGSFLNVVIHRTPLIMVSAWRKECSQFMYEQADMPR
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCH
EHTMPLVNIVATDTPITLSKPASRCPHCAHKIKWYENIPLISWIVLRGRCSECKAAIGLR
HHCCCHHHEEECCCCCEECCCHHHCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCC
YPIVELVTALLSALVIYHFGVNMVGLSALVLVWTLIALTGIDFDTQLLPDRLTFPLAGLG
CCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHCCCHHHCC
LAVNSQGWFVPPTQSIWGLLLGFLSLWIVVKIFYLITKKHGMGQGDFKLLAVLGAWLGPM
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MLPLIILLSSLLGSIVGLILMKKQGESKPFAFGPYIAIAGIVALLYGSDIVNWYLGMYT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQFIQLLQENMTIALVVFGLLGLCVGSFLNVVIHRTPLIMVSAWRKECSQFMYEQADMPR
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCH
EHTMPLVNIVATDTPITLSKPASRCPHCAHKIKWYENIPLISWIVLRGRCSECKAAIGLR
HHCCCHHHEEECCCCCEECCCHHHCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCC
YPIVELVTALLSALVIYHFGVNMVGLSALVLVWTLIALTGIDFDTQLLPDRLTFPLAGLG
CCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHCCCHHHCC
LAVNSQGWFVPPTQSIWGLLLGFLSLWIVVKIFYLITKKHGMGQGDFKLLAVLGAWLGPM
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MLPLIILLSSLLGSIVGLILMKKQGESKPFAFGPYIAIAGIVALLYGSDIVNWYLGMYT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA