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The map label for this gene is mcrA [H]

Identifier: 93004848

GI number: 93004848

Start: 22169

End: 23017

Strand: Reverse

Name: mcrA [H]

Synonym: Pcryo_0017

Alternate gene names: 93004848

Gene position: 23017-22169 (Counterclockwise)

Preceding gene: 93004849

Following gene: 93004846

Centisome position: 0.75

GC content: 37.1

Gene sequence:

>849_bases
GTGTTTGAAATAGGAGAAATTTATAACCGTCGTTCTGATATACACGGTATATATAAAGGGCAACAATATGGTGGTATAGC
AACGCCTGCCGAGCATCCATATATATTTATTTTCACCGGCGATGCTGGTGGTGAGTATGGTTATATTGATGATTTTGATC
CTAACGGCACGTTTAAATATACTGGTGAAGGGCAAGAAGGTGACATGAAAATGACCAAAGGTAATTTGGCAATTCATGAC
CATCAAAGAAACAATAAAGAAATCCTACTTTTCGAATCAACATCACGAGGATTTGTACGATTTTTAGGGTATTGCAATTA
CATTTTTCATCATATTGAAGAACGGCCAGATAGAAATAGCGAGCTTCGTGACGCTATCGTTTTTCATCTAGATATAGTTA
ATACTAAAAATATTGATAAAGCTCAAACTCTGACTTCTAAACTAGTTGAAGCCCCTAAAGCCGTTTATGTCACTAAGCCT
AGTAAAGGTAAGTCATTACAGCAACTACGAGAGATTGCCTTAAGTTCAACACCAACCCACGCAAGCACTCAAGAAAAAAT
TCAATCTATTCAAAACCGTAGTACAGCAATTAAACTGTATGCCAAAAAAAGAGCTAATGGTATATGCGAGGGATGTAATG
AGATAGCTCCATTTGAAACTAAATCTGGACCTTATCTCGAAGTCCATCATCTAACTAGGTTGGCAGATGGTGGTGCGGAC
TTACCTCAAAACGTCATCGCATTGTGCCCTACCTGTCATCGAAAAGCTCACTACTCTTTAAATCATTTAGAATTTAATAA
TCAGTTAATAAACAAAGTTGCTGCTATCGAAGCAGAATTTAGTGAGTAA

Upstream 100 bases:

>100_bases
TGTATAAGGTTGAAATTAACAATAAGAAGCATAGAAACTATTATTTATAGCGAACTTAAGTATATTCTATTCCCTTAATG
TAAATAACAAGGAAGTCATA

Downstream 100 bases:

>100_bases
AACACTTAATTTTAAACTAAAAAAACACCCAACCAAGTTGAGTGCTTTTTTTATTTCATCTGAATATCAAGCGGAGAATT
ATTCCCACTCAATCGTCGCA

Product: HNH endonuclease

Products: NA

Alternate protein names: EcoKMcrA [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MFEIGEIYNRRSDIHGIYKGQQYGGIATPAEHPYIFIFTGDAGGEYGYIDDFDPNGTFKYTGEGQEGDMKMTKGNLAIHD
HQRNNKEILLFESTSRGFVRFLGYCNYIFHHIEERPDRNSELRDAIVFHLDIVNTKNIDKAQTLTSKLVEAPKAVYVTKP
SKGKSLQQLREIALSSTPTHASTQEKIQSIQNRSTAIKLYAKKRANGICEGCNEIAPFETKSGPYLEVHHLTRLADGGAD
LPQNVIALCPTCHRKAHYSLNHLEFNNQLINKVAAIEAEFSE

Sequences:

>Translated_282_residues
MFEIGEIYNRRSDIHGIYKGQQYGGIATPAEHPYIFIFTGDAGGEYGYIDDFDPNGTFKYTGEGQEGDMKMTKGNLAIHD
HQRNNKEILLFESTSRGFVRFLGYCNYIFHHIEERPDRNSELRDAIVFHLDIVNTKNIDKAQTLTSKLVEAPKAVYVTKP
SKGKSLQQLREIALSSTPTHASTQEKIQSIQNRSTAIKLYAKKRANGICEGCNEIAPFETKSGPYLEVHHLTRLADGGAD
LPQNVIALCPTCHRKAHYSLNHLEFNNQLINKVAAIEAEFSE
>Mature_282_residues
MFEIGEIYNRRSDIHGIYKGQQYGGIATPAEHPYIFIFTGDAGGEYGYIDDFDPNGTFKYTGEGQEGDMKMTKGNLAIHD
HQRNNKEILLFESTSRGFVRFLGYCNYIFHHIEERPDRNSELRDAIVFHLDIVNTKNIDKAQTLTSKLVEAPKAVYVTKP
SKGKSLQQLREIALSSTPTHASTQEKIQSIQNRSTAIKLYAKKRANGICEGCNEIAPFETKSGPYLEVHHLTRLADGGAD
LPQNVIALCPTCHRKAHYSLNHLEFNNQLINKVAAIEAEFSE

Specific function: Restriction of 5-methyl and 5-hydroxymethylcytosines at the specific DNA sequence C(me)CGG [H]

COG id: COG1403

COG function: function code V; Restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787406, Length=124, Percent_Identity=37.0967741935484, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002711
- InterPro:   IPR003615 [H]

Pfam domain/function: PF01844 HNH [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 31737; Mature: 31737

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEIGEIYNRRSDIHGIYKGQQYGGIATPAEHPYIFIFTGDAGGEYGYIDDFDPNGTFKY
CCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
TGEGQEGDMKMTKGNLAIHDHQRNNKEILLFESTSRGFVRFLGYCNYIFHHIEERPDRNS
CCCCCCCCEEEECCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
ELRDAIVFHLDIVNTKNIDKAQTLTSKLVEAPKAVYVTKPSKGKSLQQLREIALSSTPTH
HHHHEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCC
ASTQEKIQSIQNRSTAIKLYAKKRANGICEGCNEIAPFETKSGPYLEVHHLTRLADGGAD
CHHHHHHHHHHCCCCEEEEEEHHHCCCHHHCHHCCCCCCCCCCCEEEHHHHHHHCCCCCC
LPQNVIALCPTCHRKAHYSLNHLEFNNQLINKVAAIEAEFSE
CCHHHHHHCCCHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFEIGEIYNRRSDIHGIYKGQQYGGIATPAEHPYIFIFTGDAGGEYGYIDDFDPNGTFKY
CCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
TGEGQEGDMKMTKGNLAIHDHQRNNKEILLFESTSRGFVRFLGYCNYIFHHIEERPDRNS
CCCCCCCCEEEECCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
ELRDAIVFHLDIVNTKNIDKAQTLTSKLVEAPKAVYVTKPSKGKSLQQLREIALSSTPTH
HHHHEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCC
ASTQEKIQSIQNRSTAIKLYAKKRANGICEGCNEIAPFETKSGPYLEVHHLTRLADGGAD
CHHHHHHHHHHCCCCEEEEEEHHHCCCHHHCHHCCCCCCCCCCCEEEHHHHHHHCCCCCC
LPQNVIALCPTCHRKAHYSLNHLEFNNQLINKVAAIEAEFSE
CCHHHHHHCCCHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1938927; 8905232; 9278503 [H]