Click here to switch to the map view.

The map label for this gene is metR [H]

Identifier: 92115280

GI number: 92115280

Start: 3529447

End: 3530331

Strand: Direct

Name: metR [H]

Synonym: Csal_3165

Alternate gene names: 92115280

Gene position: 3529447-3530331 (Clockwise)

Preceding gene: 92115278

Following gene: 92115281

Centisome position: 95.48

GC content: 67.46

Gene sequence:

>885_bases
ATGCTGGATATCAAGTATTACGTATTGATCGACGCCATCGCGACCCACGGCACGGTGCACGATGCCGCCGCCGCCATCGG
CATCACCCAGCCCGCGGCGACGCATCGCATTCGGGAAATGGAACGCCGCCTGGGCGTTGCGCTGTTCCATCGCGAAGGCC
GTCGGCTGACGCTTTCCGCCTCGGGCGAGCGTCTGCTCGACACCGCTCATGCGGTACTCCCCCAGTTGCGCAACGCCGAG
CTCGAGGCATGGCAGCTGGCTCGCCGCGCCGAGCCCGCCCTGCGCCTGGGCATCGGCCCCTACGACACCTTCTCGCGGGC
GATCCCGCATCTCTACGACCAGCATGAGCGCGACATCGACCTGGTGCGCTTGCCCATGGCGGACATGACCACGGCCCTGC
TCGCCAACCGGGTCGACATGATCTGCATGATCGAAGTCCCTTCGCAGCGCGGGATCGCTCACCAGTTGCTGTTCGAGGAG
CCACTGGTGGCCGTGCTGCCCCCCGACCACCCCGACGCGCAGAGCGACACCATCCCCGCCGAGGTATTCGACCGGGGACG
ACACTTCACCTACAGCATCACGCCCGAGTCCGGCCACGAGTTCGAGCACTTCTTCCAGCCCGCCGGCGTCTATCCCTCGC
ACATGGTACAGATCGAGTCGGTCGCCCTGATCCTCGAGCTGGTCGCCGCCGGCAAGGGCGTGAGCATCCTGTCGCGCTGG
GCCGCCCGGGACGCCCATCAGGCCGGGCACGTCGTCATGCGCCCCCTGGCCGGCGACATCCCGCGCATTCCCTGGTACCT
GGCCTATACGCAGACGCCCCACATCGCCGCCGTCGCCGAACCACTGGCGGACACGCTACGCCATCTCTATCGCGCCGATG
AGTGA

Upstream 100 bases:

>100_bases
TGGTGGTCCGACATCGTGTCGTGCTCCTTGTCGCATCTTGCATCGAGCGTAGCATGTCGTTTCACGCGACGCGCCACCCT
TCAAGAGACCCGAGAGTCCC

Downstream 100 bases:

>100_bases
TCGCGAGGCGCGCCGCCAAGGGCCTGAGACCGAACGATAAGAATCTCGTCGCAGATGGCCGGACATTCTCATTAGTTTCA
CGCTCCCCGGGCTTTTACCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLDIKYYVLIDAIATHGTVHDAAAAIGITQPAATHRIREMERRLGVALFHREGRRLTLSASGERLLDTAHAVLPQLRNAE
LEAWQLARRAEPALRLGIGPYDTFSRAIPHLYDQHERDIDLVRLPMADMTTALLANRVDMICMIEVPSQRGIAHQLLFEE
PLVAVLPPDHPDAQSDTIPAEVFDRGRHFTYSITPESGHEFEHFFQPAGVYPSHMVQIESVALILELVAAGKGVSILSRW
AARDAHQAGHVVMRPLAGDIPRIPWYLAYTQTPHIAAVAEPLADTLRHLYRADE

Sequences:

>Translated_294_residues
MLDIKYYVLIDAIATHGTVHDAAAAIGITQPAATHRIREMERRLGVALFHREGRRLTLSASGERLLDTAHAVLPQLRNAE
LEAWQLARRAEPALRLGIGPYDTFSRAIPHLYDQHERDIDLVRLPMADMTTALLANRVDMICMIEVPSQRGIAHQLLFEE
PLVAVLPPDHPDAQSDTIPAEVFDRGRHFTYSITPESGHEFEHFFQPAGVYPSHMVQIESVALILELVAAGKGVSILSRW
AARDAHQAGHVVMRPLAGDIPRIPWYLAYTQTPHIAAVAEPLADTLRHLYRADE
>Mature_294_residues
MLDIKYYVLIDAIATHGTVHDAAAAIGITQPAATHRIREMERRLGVALFHREGRRLTLSASGERLLDTAHAVLPQLRNAE
LEAWQLARRAEPALRLGIGPYDTFSRAIPHLYDQHERDIDLVRLPMADMTTALLANRVDMICMIEVPSQRGIAHQLLFEE
PLVAVLPPDHPDAQSDTIPAEVFDRGRHFTYSITPESGHEFEHFFQPAGVYPSHMVQIESVALILELVAAGKGVSILSRW
AARDAHQAGHVVMRPLAGDIPRIPWYLAYTQTPHIAAVAEPLADTLRHLYRADE

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=261, Percent_Identity=28.3524904214559, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788887, Length=276, Percent_Identity=29.3478260869565, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790399, Length=303, Percent_Identity=25.4125412541254, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=235, Percent_Identity=26.8085106382979, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787879, Length=255, Percent_Identity=26.2745098039216, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082132, Length=270, Percent_Identity=23.3333333333333, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787601, Length=281, Percent_Identity=22.7758007117438, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=24.9056603773585, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32815; Mature: 32815

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIKYYVLIDAIATHGTVHDAAAAIGITQPAATHRIREMERRLGVALFHREGRRLTLSA
CCCEEEEHEEEHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCEEEEEC
SGERLLDTAHAVLPQLRNAELEAWQLARRAEPALRLGIGPYDTFSRAIPHLYDQHERDID
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCCCC
LVRLPMADMTTALLANRVDMICMIEVPSQRGIAHQLLFEEPLVAVLPPDHPDAQSDTIPA
EEECCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCH
EVFDRGRHFTYSITPESGHEFEHFFQPAGVYPSHMVQIESVALILELVAAGKGVSILSRW
HHHHCCCEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AARDAHQAGHVVMRPLAGDIPRIPWYLAYTQTPHIAAVAEPLADTLRHLYRADE
HHHHHHHCCCEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDIKYYVLIDAIATHGTVHDAAAAIGITQPAATHRIREMERRLGVALFHREGRRLTLSA
CCCEEEEHEEEHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCEEEEEC
SGERLLDTAHAVLPQLRNAELEAWQLARRAEPALRLGIGPYDTFSRAIPHLYDQHERDID
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCCCC
LVRLPMADMTTALLANRVDMICMIEVPSQRGIAHQLLFEEPLVAVLPPDHPDAQSDTIPA
EEECCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCH
EVFDRGRHFTYSITPESGHEFEHFFQPAGVYPSHMVQIESVALILELVAAGKGVSILSRW
HHHHCCCEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AARDAHQAGHVVMRPLAGDIPRIPWYLAYTQTPHIAAVAEPLADTLRHLYRADE
HHHHHHHCCCEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]