Click here to switch to the map view.

The map label for this gene is ykoW [H]

Identifier: 92115148

GI number: 92115148

Start: 3378442

End: 3380775

Strand: Direct

Name: ykoW [H]

Synonym: Csal_3033

Alternate gene names: 92115148

Gene position: 3378442-3380775 (Clockwise)

Preceding gene: 92115147

Following gene: 92115150

Centisome position: 91.39

GC content: 63.07

Gene sequence:

>2334_bases
ATGCCACCGAGTAGATTTCCTGCGCTTTTCTCCCAGCCGTCGGGAGAATCCCAGAACCTGCTGCATGTGCAGGTCGCGTT
GAGGGCCCGCGCAGATCAACGATTGCTGGTGCGGACGCTGCGAGACGCCGGGTTAACGCCGGAAAGCGTGACCCTGCCAG
ATGGCAAGGCCGATCTGGTCATCGTGGACGCCTGGGCGTGGGAGCACTCCCGCGAGCGACTACGAAGCGTTCGTGAAAAC
GCCTACCCGCTGGTGTTTCCGATCCTGGCAATTGCCGGCAAGGAAGCAGCGTCCGCCGCCTGGGCCTATCGCGAACTCGG
CGCCGACATCGACGACCTGGTGGAGCTGCCCACCTCCAGGGCCGTGCTGCTGTCCCGTGTGGGCAATTTGCTGCGCCTGC
GCCGACTCTCTCTGACACAGGCCGACCACCACCGATCGACCCAGCAAAAGCTGAATGCCGTCAATCGCGCGCTGCAGACC
TTGCATGCCTGCAACGAAATGATGGTGCGTGAATCCACGGAAGAGGGGCTGTTGCAATCCGTGTGCGAGGTCATCGCGCA
ATTTGAAGGATACGCACTGGCCTGGGTCGGATTTGCCGACGACAGCACGCAGAAGAAGGTGGCCAAGTGCTACATCGCCG
GAGACGCCGCCGATTACGCGCGAGCCATCGACGTGAGTGCCGATGGCTCGCCACTGGGACAGGGCCCCATCGGGAAAGCC
ATTGCGACCGGGAGCCCCCAGATCGTTTCCGACATGGCTTCCGAACCGCGCATGGCGCCCTGGAGAGAGCAGATCACCGC
CTGGGGGCTGGGCGCGGAGATCGCGCTACCCTTGAATGTCTCGGTGGGATCGAGCGGTGTGCTGGCGGTGTATTCGCAAC
AGTCGGGAGACTTCGCCAGCGATGAACGGGCGCTGCTCGAGCGCCTCGCCGAAAACCTCGCCTTTGGCCTGGACCGCCTG
CGACTGCAGAACGTGCGCAAGGCACAGAACCAGGAAATAAAACGACTCGCCTATGAAGACCCGGTGACCGGCATGGCGAA
CCGCCGCTCCTTGCTGGCCCGTCTGCATGAACTGGTGGGACGAGCCAACCATGAGCAGGCCGCGGCGATATTGTTCATCG
ATCTCGACGACTTCAAGCTGGTGAACGATGCCCTCGGGCACAAGGTCGGCGATCGCGTCCTCCAGAACGTCGCCTGGCGC
ATCAACAATACGCTGCGGGAAGGCGACCTGGTGGCACGCCAGGGGGGTGACGAATTCATCGTCGTCATGATGGACGATCC
ACGATATTCGCCGTCAGTGGCAGAAGACGTCCGACAACGCCTGGCCCGAGGCGCCGAAGAGATGGCGCGGCGTATCAGCC
GAACGCTGCGTCAGCCTTTCGAAATCAACGGATACGCTCATCGGCTCGGTGCCAGCATCGGCGTCAGCCTCTTTCCCTAT
CTCAGCGACGACCCCGAGACCGTCATCGATCAGGCCGACATGGCGATGTATGCCGCCAAACGCTCGGGTCAGCACACGGT
CTTCTTCTCGCCGGAGCTCGCCACCGGCCGCCAGCAACGCCTGTCGTTGGAAACCAAGCTGAATCATGCGCTGGCAGCAG
GCGAGCTGCAATTGCATTACCAACCCGTCTGGGAGCTCGACAGCGGCCGAATCGTAGCCGTGGAAGCGCTTCTGCGCTGG
CAGGATGCCGAGGGCGAGATGATTTCCCCGGCGACCTTCATTCCCGTTGCCGAAGAAATCGGCTTGATGGGCGCCCTCAG
CAAATGGGTCATCGACGAGGCGGCTCGGCAGCTCGAATCCTGGCGCGAAACCGGGCTCGAACTCACCATGGGCGTCAACT
TGTCGGTATCGCAGCTTCAAGGGGCTGACGCGGCGCGCAAGATCCATGATCAGGTGGTGTCTTCCGGCAGCGACCCGCAA
TGGTGGCTACTCGAGTTGACCGAAGAAGCCGTCATGCAGTCCCCCGAGGCCGTCATGGAGGCGATGCGCCTGCTCGACGG
TGCCGGCTTCCGGCTGGCCCTGGATGACTTCGGGAGGGGTTACTCATCGCTTGCACGATTGCAGGCCATGCCACTGCACA
CCCTGAAAATCGACAAGCTGTTCGTCGACAGCCTGCGGGAAGATACGCCCGATGACCGGGTCGTGCGCACAGTGGTCGAA
CTGGCCCGGCAATTTTCCCTGCGTGTCGTGGCCGAAGGGATCGAGCATGCGGAACAGGCCCGACAATTGACCGCGATGGG
ATGCAGCTTCGGACAAGGGTTCTGGGTCAGCCCCGCCGTGGCCGCCAGCGAGATCCCCAGCTTGATACGCCGCGCCGCGC
CACCCGCGCTCTGA

Upstream 100 bases:

>100_bases
CGGGAGTTCCAGATAACCCGCGAGGGTCTGCGCGTCGGTGCACCGCTTTACGGCCTGCAGGGAATCCTGCAGGGAATCCC
TTCCAAGATAGCGACCCTTG

Downstream 100 bases:

>100_bases
ACGCACCGCCGACGCGTCGAGGCAGCGGTGACGTGCGGATCAACGACCTCCCTCTGCGTCGAGAGTCGCCATCCCCAGGC
GCCCGACGCCTGGCAACTTG

Product: GAF sensor-containing diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 777; Mature: 776

Protein sequence:

>777_residues
MPPSRFPALFSQPSGESQNLLHVQVALRARADQRLLVRTLRDAGLTPESVTLPDGKADLVIVDAWAWEHSRERLRSVREN
AYPLVFPILAIAGKEAASAAWAYRELGADIDDLVELPTSRAVLLSRVGNLLRLRRLSLTQADHHRSTQQKLNAVNRALQT
LHACNEMMVRESTEEGLLQSVCEVIAQFEGYALAWVGFADDSTQKKVAKCYIAGDAADYARAIDVSADGSPLGQGPIGKA
IATGSPQIVSDMASEPRMAPWREQITAWGLGAEIALPLNVSVGSSGVLAVYSQQSGDFASDERALLERLAENLAFGLDRL
RLQNVRKAQNQEIKRLAYEDPVTGMANRRSLLARLHELVGRANHEQAAAILFIDLDDFKLVNDALGHKVGDRVLQNVAWR
INNTLREGDLVARQGGDEFIVVMMDDPRYSPSVAEDVRQRLARGAEEMARRISRTLRQPFEINGYAHRLGASIGVSLFPY
LSDDPETVIDQADMAMYAAKRSGQHTVFFSPELATGRQQRLSLETKLNHALAAGELQLHYQPVWELDSGRIVAVEALLRW
QDAEGEMISPATFIPVAEEIGLMGALSKWVIDEAARQLESWRETGLELTMGVNLSVSQLQGADAARKIHDQVVSSGSDPQ
WWLLELTEEAVMQSPEAVMEAMRLLDGAGFRLALDDFGRGYSSLARLQAMPLHTLKIDKLFVDSLREDTPDDRVVRTVVE
LARQFSLRVVAEGIEHAEQARQLTAMGCSFGQGFWVSPAVAASEIPSLIRRAAPPAL

Sequences:

>Translated_777_residues
MPPSRFPALFSQPSGESQNLLHVQVALRARADQRLLVRTLRDAGLTPESVTLPDGKADLVIVDAWAWEHSRERLRSVREN
AYPLVFPILAIAGKEAASAAWAYRELGADIDDLVELPTSRAVLLSRVGNLLRLRRLSLTQADHHRSTQQKLNAVNRALQT
LHACNEMMVRESTEEGLLQSVCEVIAQFEGYALAWVGFADDSTQKKVAKCYIAGDAADYARAIDVSADGSPLGQGPIGKA
IATGSPQIVSDMASEPRMAPWREQITAWGLGAEIALPLNVSVGSSGVLAVYSQQSGDFASDERALLERLAENLAFGLDRL
RLQNVRKAQNQEIKRLAYEDPVTGMANRRSLLARLHELVGRANHEQAAAILFIDLDDFKLVNDALGHKVGDRVLQNVAWR
INNTLREGDLVARQGGDEFIVVMMDDPRYSPSVAEDVRQRLARGAEEMARRISRTLRQPFEINGYAHRLGASIGVSLFPY
LSDDPETVIDQADMAMYAAKRSGQHTVFFSPELATGRQQRLSLETKLNHALAAGELQLHYQPVWELDSGRIVAVEALLRW
QDAEGEMISPATFIPVAEEIGLMGALSKWVIDEAARQLESWRETGLELTMGVNLSVSQLQGADAARKIHDQVVSSGSDPQ
WWLLELTEEAVMQSPEAVMEAMRLLDGAGFRLALDDFGRGYSSLARLQAMPLHTLKIDKLFVDSLREDTPDDRVVRTVVE
LARQFSLRVVAEGIEHAEQARQLTAMGCSFGQGFWVSPAVAASEIPSLIRRAAPPAL
>Mature_776_residues
PPSRFPALFSQPSGESQNLLHVQVALRARADQRLLVRTLRDAGLTPESVTLPDGKADLVIVDAWAWEHSRERLRSVRENA
YPLVFPILAIAGKEAASAAWAYRELGADIDDLVELPTSRAVLLSRVGNLLRLRRLSLTQADHHRSTQQKLNAVNRALQTL
HACNEMMVRESTEEGLLQSVCEVIAQFEGYALAWVGFADDSTQKKVAKCYIAGDAADYARAIDVSADGSPLGQGPIGKAI
ATGSPQIVSDMASEPRMAPWREQITAWGLGAEIALPLNVSVGSSGVLAVYSQQSGDFASDERALLERLAENLAFGLDRLR
LQNVRKAQNQEIKRLAYEDPVTGMANRRSLLARLHELVGRANHEQAAAILFIDLDDFKLVNDALGHKVGDRVLQNVAWRI
NNTLREGDLVARQGGDEFIVVMMDDPRYSPSVAEDVRQRLARGAEEMARRISRTLRQPFEINGYAHRLGASIGVSLFPYL
SDDPETVIDQADMAMYAAKRSGQHTVFFSPELATGRQQRLSLETKLNHALAAGELQLHYQPVWELDSGRIVAVEALLRWQ
DAEGEMISPATFIPVAEEIGLMGALSKWVIDEAARQLESWRETGLELTMGVNLSVSQLQGADAARKIHDQVVSSGSDPQW
WLLELTEEAVMQSPEAVMEAMRLLDGAGFRLALDDFGRGYSSLARLQAMPLHTLKIDKLFVDSLREDTPDDRVVRTVVEL
ARQFSLRVVAEGIEHAEQARQLTAMGCSFGQGFWVSPAVAASEIPSLIRRAAPPAL

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=444, Percent_Identity=35.3603603603604, Blast_Score=241, Evalue=2e-64,
Organism=Escherichia coli, GI87081921, Length=492, Percent_Identity=29.8780487804878, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI226510982, Length=461, Percent_Identity=29.0672451193059, Blast_Score=173, Evalue=4e-44,
Organism=Escherichia coli, GI1790496, Length=246, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI87081845, Length=250, Percent_Identity=32.8, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI87081743, Length=235, Percent_Identity=33.6170212765957, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1786507, Length=277, Percent_Identity=31.7689530685921, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI87081980, Length=256, Percent_Identity=30.078125, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1787055, Length=240, Percent_Identity=28.75, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788502, Length=249, Percent_Identity=29.3172690763052, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1788381, Length=522, Percent_Identity=25.8620689655172, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1788849, Length=439, Percent_Identity=24.8291571753986, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI87081977, Length=252, Percent_Identity=30.1587301587302, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI87082096, Length=247, Percent_Identity=29.5546558704453, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1788956, Length=207, Percent_Identity=30.9178743961353, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87082007, Length=178, Percent_Identity=34.8314606741573, Blast_Score=83, Evalue=8e-17,
Organism=Escherichia coli, GI145693134, Length=169, Percent_Identity=34.3195266272189, Blast_Score=83, Evalue=8e-17,
Organism=Escherichia coli, GI1786584, Length=195, Percent_Identity=32.3076923076923, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1787262, Length=179, Percent_Identity=31.2849162011173, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI87081881, Length=187, Percent_Identity=28.8770053475936, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787802, Length=147, Percent_Identity=29.9319727891156, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081974, Length=125, Percent_Identity=31.2, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787816, Length=180, Percent_Identity=31.1111111111111, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 85570; Mature: 85439

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSRFPALFSQPSGESQNLLHVQVALRARADQRLLVRTLRDAGLTPESVTLPDGKADLV
CCCCCCCHHHCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCCCCEECCCCCCCEE
IVDAWAWEHSRERLRSVRENAYPLVFPILAIAGKEAASAAWAYRELGADIDDLVELPTSR
EEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHCCCHH
AVLLSRVGNLLRLRRLSLTQADHHRSTQQKLNAVNRALQTLHACNEMMVRESTEEGLLQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VCEVIAQFEGYALAWVGFADDSTQKKVAKCYIAGDAADYARAIDVSADGSPLGQGPIGKA
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHEEECCHHHHHHEEECCCCCCCCCCCCCCCC
IATGSPQIVSDMASEPRMAPWREQITAWGLGAEIALPLNVSVGSSGVLAVYSQQSGDFAS
CCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCC
DERALLERLAENLAFGLDRLRLQNVRKAQNQEIKRLAYEDPVTGMANRRSLLARLHELVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
RANHEQAAAILFIDLDDFKLVNDALGHKVGDRVLQNVAWRINNTLREGDLVARQGGDEFI
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCEECCCCCEE
VVMMDDPRYSPSVAEDVRQRLARGAEEMARRISRTLRQPFEINGYAHRLGASIGVSLFPY
EEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEECCC
LSDDPETVIDQADMAMYAAKRSGQHTVFFSPELATGRQQRLSLETKLNHALAAGELQLHY
CCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEE
QPVWELDSGRIVAVEALLRWQDAEGEMISPATFIPVAEEIGLMGALSKWVIDEAARQLES
CEEEECCCCCEEEEHHHHHHCCCCCCEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
WRETGLELTMGVNLSVSQLQGADAARKIHDQVVSSGSDPQWWLLELTEEAVMQSPEAVME
HHHCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCHHHHHH
AMRLLDGAGFRLALDDFGRGYSSLARLQAMPLHTLKIDKLFVDSLREDTPDDRVVRTVVE
HHHHHCCCCCEEEHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LARQFSLRVVAEGIEHAEQARQLTAMGCSFGQGFWVSPAVAASEIPSLIRRAAPPAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PPSRFPALFSQPSGESQNLLHVQVALRARADQRLLVRTLRDAGLTPESVTLPDGKADLV
CCCCCCHHHCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCCCCEECCCCCCCEE
IVDAWAWEHSRERLRSVRENAYPLVFPILAIAGKEAASAAWAYRELGADIDDLVELPTSR
EEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHCCCHH
AVLLSRVGNLLRLRRLSLTQADHHRSTQQKLNAVNRALQTLHACNEMMVRESTEEGLLQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VCEVIAQFEGYALAWVGFADDSTQKKVAKCYIAGDAADYARAIDVSADGSPLGQGPIGKA
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHEEECCHHHHHHEEECCCCCCCCCCCCCCCC
IATGSPQIVSDMASEPRMAPWREQITAWGLGAEIALPLNVSVGSSGVLAVYSQQSGDFAS
CCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCC
DERALLERLAENLAFGLDRLRLQNVRKAQNQEIKRLAYEDPVTGMANRRSLLARLHELVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
RANHEQAAAILFIDLDDFKLVNDALGHKVGDRVLQNVAWRINNTLREGDLVARQGGDEFI
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCEECCCCCEE
VVMMDDPRYSPSVAEDVRQRLARGAEEMARRISRTLRQPFEINGYAHRLGASIGVSLFPY
EEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEECCC
LSDDPETVIDQADMAMYAAKRSGQHTVFFSPELATGRQQRLSLETKLNHALAAGELQLHY
CCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEE
QPVWELDSGRIVAVEALLRWQDAEGEMISPATFIPVAEEIGLMGALSKWVIDEAARQLES
CEEEECCCCCEEEEHHHHHHCCCCCCEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
WRETGLELTMGVNLSVSQLQGADAARKIHDQVVSSGSDPQWWLLELTEEAVMQSPEAVME
HHHCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCHHHHHH
AMRLLDGAGFRLALDDFGRGYSSLARLQAMPLHTLKIDKLFVDSLREDTPDDRVVRTVVE
HHHHHCCCCCEEEHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LARQFSLRVVAEGIEHAEQARQLTAMGCSFGQGFWVSPAVAASEIPSLIRRAAPPAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]