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The map label for this gene is yqhT [H]

Identifier: 92115001

GI number: 92115001

Start: 3214751

End: 3216028

Strand: Direct

Name: yqhT [H]

Synonym: Csal_2884

Alternate gene names: 92115001

Gene position: 3214751-3216028 (Clockwise)

Preceding gene: 92115000

Following gene: 92115002

Centisome position: 86.96

GC content: 64.63

Gene sequence:

>1278_bases
ATGACCCGCTTGTCATCCACGCTGCCTTCCCTGCTGGAAGGCACCGATATCCTGGCGCCGACGCACGAACCCGCCGAGTG
CGAGCCCACTCAGGCCGAGAGCCGCCAGATTCTGCTCGACCGCCTGGCCCGCACCCGGGCCGAACTGAAGAAACGCGATA
TCGTCGCCGCCGTGCTGTTCGACCCGACCCATGTGCGCTACGCGACCGGCGCCCGCAACATGCAGGTCTACTCGGCGCGC
AACCCGGCGCGTTACGCCTTCGTGCCGGCCGAGGGCCCGGTGGTGATGTTCGAGTTCTCCGGCTGCAGTCACTTGACCGA
AGGGCTGCCGGGCATCGACGAGACACGCCCGGCGACGGCGATCTCCTATTATTTCTGTGAAGACAACCTTCAGCAGACCA
CCGAGGCGTGGGCGGACGAGATCGACGAACTGGTACGCCGATGCGGCGGACAAAAAGGCGACAAGGTGGCCATCGAAAGC
GCCACCTCGCATGCCGCCTTCGCGCTGGTCGAACGCGGCTGGCAGATCTGCGATGCCCAGATCCCGCTGGAGCGGGCGCG
GGCGATCAAGGTCCCCAACGAGATCAAGATGGTGCGCAGCTCGCTGCGCGCGGTCGAGGACGGGGTCCGCAAGCTGGAAG
CGGCGATTCGCCCCGGCGCCACGGAGAACGAGGGCTGGTCGCAGCTGCACCAGCACATCATCGCCACCGATGCGGACTAC
GTCGAAACCCGGCTGATGAATTCCGGGCCGCGCACCAACCCCTGGTTCCAGGAGTGCTCCAATAGAGCGATGCAGGCCGG
CGAGCTGGTCGCGCTGGATACCGACGTGGTCGGCCGTTATGGTTACTACGCCGATTTCTCGCGAACCTTCCTGTGCGGCG
ACGGCCAGGCGTCGTCCACCCAGCGCGAGTTGTATCGCATGGCCTTCGATCAGGTACGCACCAACATGGCCCTGATCGCG
CCGGGCATGTCGTTCAAGGAATACGCCGAGAAGGCCTGGCAGATTCCCCAACCCTACAAGGCGCGCCGCTATTTCGCGCT
GGCGCATGGGGTCGGCATGACCGGCGAGTATCCGTATATCGTTCACCGCGAGGACATCGACGACAAGGGCTACGACGGCA
TCATGCAACCCGGCATGACGCTTTGCGTGGAAAGCTATATCGGCCACGAGGCCGGCGGCGAAGGCGTCAAGCTCGAGGAG
CAGCTTTACATCCGCGACGATGGCCAGGTCGAATTGCTTTCCGACTATCCACTCGATACGCGGCTGCTGGCCGACTGA

Upstream 100 bases:

>100_bases
CGAGGCGGAAAAGCGCCGTGAAGTTCGCGCCGACGGGCTGCTCGACTGATCGAAATGCCTGGAACGGGCTGATTCAATTT
CCATGACGGGACCGACGACC

Downstream 100 bases:

>100_bases
GGGCCAGTCGTCTCATCCCGACAGAAGCGAACGCCATGACCCAGACATCGATCCCGCATATCGATCTCACGCCACTTCTC
GAAGGCGCCGGCAGCGACGA

Product: peptidase M24

Products: N-terminal amino acid including proline [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTRLSSTLPSLLEGTDILAPTHEPAECEPTQAESRQILLDRLARTRAELKKRDIVAAVLFDPTHVRYATGARNMQVYSAR
NPARYAFVPAEGPVVMFEFSGCSHLTEGLPGIDETRPATAISYYFCEDNLQQTTEAWADEIDELVRRCGGQKGDKVAIES
ATSHAAFALVERGWQICDAQIPLERARAIKVPNEIKMVRSSLRAVEDGVRKLEAAIRPGATENEGWSQLHQHIIATDADY
VETRLMNSGPRTNPWFQECSNRAMQAGELVALDTDVVGRYGYYADFSRTFLCGDGQASSTQRELYRMAFDQVRTNMALIA
PGMSFKEYAEKAWQIPQPYKARRYFALAHGVGMTGEYPYIVHREDIDDKGYDGIMQPGMTLCVESYIGHEAGGEGVKLEE
QLYIRDDGQVELLSDYPLDTRLLAD

Sequences:

>Translated_425_residues
MTRLSSTLPSLLEGTDILAPTHEPAECEPTQAESRQILLDRLARTRAELKKRDIVAAVLFDPTHVRYATGARNMQVYSAR
NPARYAFVPAEGPVVMFEFSGCSHLTEGLPGIDETRPATAISYYFCEDNLQQTTEAWADEIDELVRRCGGQKGDKVAIES
ATSHAAFALVERGWQICDAQIPLERARAIKVPNEIKMVRSSLRAVEDGVRKLEAAIRPGATENEGWSQLHQHIIATDADY
VETRLMNSGPRTNPWFQECSNRAMQAGELVALDTDVVGRYGYYADFSRTFLCGDGQASSTQRELYRMAFDQVRTNMALIA
PGMSFKEYAEKAWQIPQPYKARRYFALAHGVGMTGEYPYIVHREDIDDKGYDGIMQPGMTLCVESYIGHEAGGEGVKLEE
QLYIRDDGQVELLSDYPLDTRLLAD
>Mature_424_residues
TRLSSTLPSLLEGTDILAPTHEPAECEPTQAESRQILLDRLARTRAELKKRDIVAAVLFDPTHVRYATGARNMQVYSARN
PARYAFVPAEGPVVMFEFSGCSHLTEGLPGIDETRPATAISYYFCEDNLQQTTEAWADEIDELVRRCGGQKGDKVAIESA
TSHAAFALVERGWQICDAQIPLERARAIKVPNEIKMVRSSLRAVEDGVRKLEAAIRPGATENEGWSQLHQHIIATDADYV
ETRLMNSGPRTNPWFQECSNRAMQAGELVALDTDVVGRYGYYADFSRTFLCGDGQASSTQRELYRMAFDQVRTNMALIAP
GMSFKEYAEKAWQIPQPYKARRYFALAHGVGMTGEYPYIVHREDIDDKGYDGIMQPGMTLCVESYIGHEAGGEGVKLEEQ
LYIRDDGQVELLSDYPLDTRLLAD

Specific function: Unknown

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR001131 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.11.9 [C]

Molecular weight: Translated: 47524; Mature: 47393

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLSSTLPSLLEGTDILAPTHEPAECEPTQAESRQILLDRLARTRAELKKRDIVAAVLF
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
DPTHVRYATGARNMQVYSARNPARYAFVPAEGPVVMFEFSGCSHLTEGLPGIDETRPATA
CCCCEEEECCCCCEEEEECCCCCEEEEEECCCCEEEEEECCCHHHHHCCCCCCCCCCCCE
ISYYFCEDNLQQTTEAWADEIDELVRRCGGQKGDKVAIESATSHAAFALVERGWQICDAQ
EEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCHHEECCC
IPLERARAIKVPNEIKMVRSSLRAVEDGVRKLEAAIRPGATENEGWSQLHQHIIATDADY
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHH
VETRLMNSGPRTNPWFQECSNRAMQAGELVALDTDVVGRYGYYADFSRTFLCGDGQASST
HHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHCCCCEECCCCEEEECCCCCCHH
QRELYRMAFDQVRTNMALIAPGMSFKEYAEKAWQIPQPYKARRYFALAHGVGMTGEYPYI
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCHHHHHEEHHECCCCCCCCCCEE
VHREDIDDKGYDGIMQPGMTLCVESYIGHEAGGEGVKLEEQLYIRDDGQVELLSDYPLDT
EECCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCC
RLLAD
EECCC
>Mature Secondary Structure 
TRLSSTLPSLLEGTDILAPTHEPAECEPTQAESRQILLDRLARTRAELKKRDIVAAVLF
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
DPTHVRYATGARNMQVYSARNPARYAFVPAEGPVVMFEFSGCSHLTEGLPGIDETRPATA
CCCCEEEECCCCCEEEEECCCCCEEEEEECCCCEEEEEECCCHHHHHCCCCCCCCCCCCE
ISYYFCEDNLQQTTEAWADEIDELVRRCGGQKGDKVAIESATSHAAFALVERGWQICDAQ
EEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCHHEECCC
IPLERARAIKVPNEIKMVRSSLRAVEDGVRKLEAAIRPGATENEGWSQLHQHIIATDADY
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHH
VETRLMNSGPRTNPWFQECSNRAMQAGELVALDTDVVGRYGYYADFSRTFLCGDGQASST
HHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHCCCCEECCCCEEEECCCCCCHH
QRELYRMAFDQVRTNMALIAPGMSFKEYAEKAWQIPQPYKARRYFALAHGVGMTGEYPYI
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCHHHHHEEHHECCCCCCCCCCEE
VHREDIDDKGYDGIMQPGMTLCVESYIGHEAGGEGVKLEEQLYIRDDGQVELLSDYPLDT
EECCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCC
RLLAD
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+ [C]

Kcat value (1/min): 8100 [C]

Specific activity: 3.45

Km value (mM): 3 {(4-nitro)Phe-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} 0.22 {(4-nitro)Phe-Pro-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} [C]

Substrates: N-terminal amino acid including proline [C]

Specific reaction: Release of any N-terminal amino acid including proline that mislinked with proline even from a dipeptide or tripeptide [C]

General reaction: carboxylic acid amide hydrolysis [C]

Inhibitor: thiazolidide acid; -2-hydroxy -3-amino acidpyrrolidide acid; Pro-methyl ester acid; Pro-Phe-methyl ester acid; 2-hydroxy -3-aminoacyl-Pro-OH dipeptides [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]