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The map label for this gene is nfsA [C]

Identifier: 92114994

GI number: 92114994

Start: 3209878

End: 3210489

Strand: Direct

Name: nfsA [C]

Synonym: Csal_2877

Alternate gene names: 92114994

Gene position: 3209878-3210489 (Clockwise)

Preceding gene: 92114993

Following gene: 92114995

Centisome position: 86.83

GC content: 60.13

Gene sequence:

>612_bases
ATGCAAACCAAGGAGAGAAATGTGGCCTCGTTCGAGCACACCATCCGTTCGCGTTATAGCTGTCGTGATTTCAGCCCGCA
AACGGTTCCTCGCGCACTCCTCGACAGACTGCTAAGGGCCGCGCAGGGGATCACCCATGCCGATGGCAAACGCAGCGTGC
CTTCGGCGAGTTCTCTCTACTCTCTCAAGCTATTAGCGATAGCCGGGCGCATTCGCGGCCTGGACGCGGCGGCCTACATC
TTCGACCCCGAAAGCTGCAGGTTGGAAACGTACCTCGACCACGACGTACGATATGCACTCCAGCACGCGGCACTCGAGGA
GCAACCTTGGATCGGCCAGGCGGCCGGCATTATCACGATCTGTGCCGACATGGGAGCCGCAACCCAAACATTCGCCGATC
AACCACCCTATGGATCACGCGGCCAACGCTATGCTTGGATCGAGGCAGGCGCCGCAGCTCAGAACATTCAGTTGCAAGCA
ACTGCCGAGGAGCTGGGTAGCGTGCTCGTCGCCGGCTTCCGCGACGAGGCCACGGCAGAAGCGCTGGTCCTCGAAGCGCC
TATAACGCCGCTAATCCATTTGTGCTTCGGTTGGCCGGCTGCTAAGCAGTAA

Upstream 100 bases:

>100_bases
AGCATCTCTTGGCACGGCGATCGGCCATACCCGACGACCTGTCTTGGATGACCCGAACTGTCGGCATCATCCTCGGGCAG
AGCTACGCACCGCCTAATCC

Downstream 100 bases:

>100_bases
CGGGCCCCGACGGAATAAACGATGTCCGAGAGCCACTGACGGTTTTCCACCAAGGAAGGAGTGACCATGATCCGCGTCCC
ATTATTCTGCAGTGGCCTGA

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MQTKERNVASFEHTIRSRYSCRDFSPQTVPRALLDRLLRAAQGITHADGKRSVPSASSLYSLKLLAIAGRIRGLDAAAYI
FDPESCRLETYLDHDVRYALQHAALEEQPWIGQAAGIITICADMGAATQTFADQPPYGSRGQRYAWIEAGAAAQNIQLQA
TAEELGSVLVAGFRDEATAEALVLEAPITPLIHLCFGWPAAKQ

Sequences:

>Translated_203_residues
MQTKERNVASFEHTIRSRYSCRDFSPQTVPRALLDRLLRAAQGITHADGKRSVPSASSLYSLKLLAIAGRIRGLDAAAYI
FDPESCRLETYLDHDVRYALQHAALEEQPWIGQAAGIITICADMGAATQTFADQPPYGSRGQRYAWIEAGAAAQNIQLQA
TAEELGSVLVAGFRDEATAEALVLEAPITPLIHLCFGWPAAKQ
>Mature_203_residues
MQTKERNVASFEHTIRSRYSCRDFSPQTVPRALLDRLLRAAQGITHADGKRSVPSASSLYSLKLLAIAGRIRGLDAAAYI
FDPESCRLETYLDHDVRYALQHAALEEQPWIGQAAGIITICADMGAATQTFADQPPYGSRGQRYAWIEAGAAAQNIQLQA
TAEELGSVLVAGFRDEATAEALVLEAPITPLIHLCFGWPAAKQ

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415
- InterPro:   IPR020051 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 22127; Mature: 22127

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKERNVASFEHTIRSRYSCRDFSPQTVPRALLDRLLRAAQGITHADGKRSVPSASSLY
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
SLKLLAIAGRIRGLDAAAYIFDPESCRLETYLDHDVRYALQHAALEEQPWIGQAAGIITI
HHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
CADMGAATQTFADQPPYGSRGQRYAWIEAGAAAQNIQLQATAEELGSVLVAGFRDEATAE
HHCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHCCCCCCCHH
ALVLEAPITPLIHLCFGWPAAKQ
EEEEECCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQTKERNVASFEHTIRSRYSCRDFSPQTVPRALLDRLLRAAQGITHADGKRSVPSASSLY
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
SLKLLAIAGRIRGLDAAAYIFDPESCRLETYLDHDVRYALQHAALEEQPWIGQAAGIITI
HHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
CADMGAATQTFADQPPYGSRGQRYAWIEAGAAAQNIQLQATAEELGSVLVAGFRDEATAE
HHCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHCCCCCCCHH
ALVLEAPITPLIHLCFGWPAAKQ
EEEEECCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]