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The map label for this gene is ycdT [H]

Identifier: 92114993

GI number: 92114993

Start: 3208613

End: 3209770

Strand: Direct

Name: ycdT [H]

Synonym: Csal_2876

Alternate gene names: 92114993

Gene position: 3208613-3209770 (Clockwise)

Preceding gene: 92114992

Following gene: 92114994

Centisome position: 86.8

GC content: 59.84

Gene sequence:

>1158_bases
ATGCCAGACAAGCTTTACATGCACCACAACGCCTACTCAATTCCGCGCTGGCGCTTCATGTACTGGTTGCTCTGCGACAG
CCGCTTGCCCTTGAATATTCAACAGCGCCTGCTGCTGGGCCCCAACACCCAGACATCCGTCATGTGGTCCGGGACGCTCA
CGGTACTCGTACTGACGCTCATCGCCCTCTCCCGTCATCCGGCACCGCTATTCTGGCTCTGGTTTCTGGCCACGCTCGCG
ATCTCGGGTTACCGCGCCGGGCTACATTACCATGTCAAGCGGCAATGGCGCTGGCGTATCGTCACACCCGCCGATCAGAT
CTATCTGGCCAATCTGCTCTGGAGTACCACGACCGGGCTGGGAACCGCGCTTTGCCTGCTCAGCGGCGATCCGGTACTAC
AAGTATTGGCCATCCCATCCATGGTCGCACTGGCATCGGCCGCAGCCGCTTTCTCTCACGGGACTCCCCGTTATGCCATG
TTGCAAGTCCTGCTGCTGGACCTGCCTCTCAAACTCGCGGTTCCCTTCCAGCCTGAGCCCTGGTTCTGGGTATTCGTGCT
GCAGGGCCCCTTCTTCTGGATGGGGCTGCACATGATGATCCATTCCCTTTACGACATGTCGACCCAGGCCCTGCTCGGGG
AGTATCACAGCCAGCGGCAAGCCATCCTCGACCCGCTGACCGAGCTGTTCAACCGGGAAGGCTGGTTCCGCGAGGCCCTG
ACGATCCGTGGGCCAGGGCGCGTGCCACTTCCCGTCAGCCTGCTCTTTTCGGACCTAGACGGTTTCAAGCACGTCAACGA
CACCTACGGCCACATGATCGGCGATGATCTCCTTCGCGACATTGCCAGTGCAATGAGCGAGGCATTGAGAAGTGACGACA
TCCTCGCACGCTGGGGCGGTGATGAATTCGTGATCTTGCTACCAGGTATCAGCCCCGAGGCAAGTCGCACCGTTGCACAA
CGACTCATCGATACCACTGCCGACATCGGGCGTACCTATCCGGGAATAGGACTGAGCATCGGACTCGTGCATTGTCAGAA
TTGGCGAGGCATGGATCGCAACCGCCTGGAGCGTCTGGTCGCGCAGGCGGATCAATCCCTCTACCGAGCCAAGCGGCAAG
GCAAGGGATGCTTTCAAGAACTGACCGAGCAGGTCTGA

Upstream 100 bases:

>100_bases
CACAGGCACGCTCAGCCCTGAGAACAAAAGCGGCGACGTTTTGCAGCATGCGCCAGCCCCGTCGCCACGCAAGGACGTTT
GATCGATGAGGGCCGCCCAT

Downstream 100 bases:

>100_bases
GCCCGCAAGCATCTCTTGGCACGGCGATCGGCCATACCCGACGACCTGTCTTGGATGACCCGAACTGTCGGCATCATCCT
CGGGCAGAGCTACGCACCGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MPDKLYMHHNAYSIPRWRFMYWLLCDSRLPLNIQQRLLLGPNTQTSVMWSGTLTVLVLTLIALSRHPAPLFWLWFLATLA
ISGYRAGLHYHVKRQWRWRIVTPADQIYLANLLWSTTTGLGTALCLLSGDPVLQVLAIPSMVALASAAAAFSHGTPRYAM
LQVLLLDLPLKLAVPFQPEPWFWVFVLQGPFFWMGLHMMIHSLYDMSTQALLGEYHSQRQAILDPLTELFNREGWFREAL
TIRGPGRVPLPVSLLFSDLDGFKHVNDTYGHMIGDDLLRDIASAMSEALRSDDILARWGGDEFVILLPGISPEASRTVAQ
RLIDTTADIGRTYPGIGLSIGLVHCQNWRGMDRNRLERLVAQADQSLYRAKRQGKGCFQELTEQV

Sequences:

>Translated_385_residues
MPDKLYMHHNAYSIPRWRFMYWLLCDSRLPLNIQQRLLLGPNTQTSVMWSGTLTVLVLTLIALSRHPAPLFWLWFLATLA
ISGYRAGLHYHVKRQWRWRIVTPADQIYLANLLWSTTTGLGTALCLLSGDPVLQVLAIPSMVALASAAAAFSHGTPRYAM
LQVLLLDLPLKLAVPFQPEPWFWVFVLQGPFFWMGLHMMIHSLYDMSTQALLGEYHSQRQAILDPLTELFNREGWFREAL
TIRGPGRVPLPVSLLFSDLDGFKHVNDTYGHMIGDDLLRDIASAMSEALRSDDILARWGGDEFVILLPGISPEASRTVAQ
RLIDTTADIGRTYPGIGLSIGLVHCQNWRGMDRNRLERLVAQADQSLYRAKRQGKGCFQELTEQV
>Mature_384_residues
PDKLYMHHNAYSIPRWRFMYWLLCDSRLPLNIQQRLLLGPNTQTSVMWSGTLTVLVLTLIALSRHPAPLFWLWFLATLAI
SGYRAGLHYHVKRQWRWRIVTPADQIYLANLLWSTTTGLGTALCLLSGDPVLQVLAIPSMVALASAAAAFSHGTPRYAML
QVLLLDLPLKLAVPFQPEPWFWVFVLQGPFFWMGLHMMIHSLYDMSTQALLGEYHSQRQAILDPLTELFNREGWFREALT
IRGPGRVPLPVSLLFSDLDGFKHVNDTYGHMIGDDLLRDIASAMSEALRSDDILARWGGDEFVILLPGISPEASRTVAQR
LIDTTADIGRTYPGIGLSIGLVHCQNWRGMDRNRLERLVAQADQSLYRAKRQGKGCFQELTEQV

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=159, Percent_Identity=35.2201257861635, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=194, Percent_Identity=31.9587628865979, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=36.7088607594937, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=34.8837209302326, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082007, Length=190, Percent_Identity=32.1052631578947, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787541, Length=131, Percent_Identity=37.4045801526718, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=26.219512195122, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787816, Length=136, Percent_Identity=30.1470588235294, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1786584, Length=187, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=26.9938650306748, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=27.3291925465839, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=162, Percent_Identity=31.4814814814815, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 43652; Mature: 43521

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDKLYMHHNAYSIPRWRFMYWLLCDSRLPLNIQQRLLLGPNTQTSVMWSGTLTVLVLTL
CCCCEEEECCCCCCCHHHHHHHHEECCCCCCCHHHHEEECCCCCCEEEECCHHHHHHHHH
IALSRHPAPLFWLWFLATLAISGYRAGLHYHVKRQWRWRIVTPADQIYLANLLWSTTTGL
HHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHCCH
GTALCLLSGDPVLQVLAIPSMVALASAAAAFSHGTPRYAMLQVLLLDLPLKLAVPFQPEP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCC
WFWVFVLQGPFFWMGLHMMIHSLYDMSTQALLGEYHSQRQAILDPLTELFNREGWFREAL
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHE
TIRGPGRVPLPVSLLFSDLDGFKHVNDTYGHMIGDDLLRDIASAMSEALRSDDILARWGG
EECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DEFVILLPGISPEASRTVAQRLIDTTADIGRTYPGIGLSIGLVHCQNWRGMDRNRLERLV
CEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
AQADQSLYRAKRQGKGCFQELTEQV
HHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
PDKLYMHHNAYSIPRWRFMYWLLCDSRLPLNIQQRLLLGPNTQTSVMWSGTLTVLVLTL
CCCEEEECCCCCCCHHHHHHHHEECCCCCCCHHHHEEECCCCCCEEEECCHHHHHHHHH
IALSRHPAPLFWLWFLATLAISGYRAGLHYHVKRQWRWRIVTPADQIYLANLLWSTTTGL
HHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHCCH
GTALCLLSGDPVLQVLAIPSMVALASAAAAFSHGTPRYAMLQVLLLDLPLKLAVPFQPEP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCC
WFWVFVLQGPFFWMGLHMMIHSLYDMSTQALLGEYHSQRQAILDPLTELFNREGWFREAL
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHE
TIRGPGRVPLPVSLLFSDLDGFKHVNDTYGHMIGDDLLRDIASAMSEALRSDDILARWGG
EECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DEFVILLPGISPEASRTVAQRLIDTTADIGRTYPGIGLSIGLVHCQNWRGMDRNRLERLV
CEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
AQADQSLYRAKRQGKGCFQELTEQV
HHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]