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The map label for this gene is ispG

Identifier: 92114971

GI number: 92114971

Start: 3184555

End: 3185670

Strand: Direct

Name: ispG

Synonym: Csal_2854

Alternate gene names: 92114971

Gene position: 3184555-3185670 (Clockwise)

Preceding gene: 92114970

Following gene: 92114972

Centisome position: 86.15

GC content: 64.96

Gene sequence:

>1116_bases
ATGCATATGCAATCTCCGATCCAACGGCGCAAGTCGCGCCAGATTCATGTCGGGCACGTCCCCGTCGGTGGGGACGCCCC
CATCGCCGTGCAAAGCATGACCAATACCGACACGCTGGATGTCGACGCCACCGTCGCGCAGATTCGTCAGCTCGAGGCAG
CGGGCGCGGACATCGTGCGCGTCTCGGTGCCCACCATGGATGCCGCCGAGGCCTTCGGCCAGATCAAGCGCCAGGTCTCG
GTGCCCCTGGTGGCCGACATCCACTTCGATTACAAGATCGCCCTGCGCGTGGCCGAGCTGGGCGTCGACTGTCTGCGCAT
CAACCCCGGCAACATCGGCCGCGAGGACCGCGTGCGTGCAGTGGTCGATGCCGCACGGCATCACGGCATTCCCATTCGCA
TCGGCGTCAACGCCGGGTCGCTGGAAAAGGACCTGCAGAAGAAATACGGTGAACCCACGCCGGAGGCGCTGGTCGAATCC
GCCATGCGCCATATCGAGCACCTCGATCGCCTCGACTTCCAGGACTTCAAGGTCAGCGTCAAGGCATCGGACGTGTTCAT
GGCGGTGGCCGCCTATCGCCAGCTCGCCCAGCGCATCGATCAGCCGCTGCACCTCGGCATCACCGAGGCCGGCGGCCTGC
GGTCGGGCACGGTCAAATCCTCGATCGGCCTGGGGCTGCTGCTGATGGAAGGCATCGGCGACACCATCCGCGTCTCGCTG
GCCGCCGACCCGGTCGAGGAGATCAAGGTCGGCTATGACATGCTCAAGAGCCTGCGCCTGCGCAGCAAGGGCATCAACTT
CATCGCCTGCCCGAGTTGCTCGCGGCAGAATTTCGACGTCATCGGCACCATGAACGCCCTCGAGGAACGCCTCGACGACA
TCATGACGCCCCTCGACGTCTCGGTCATCGGCTGCGTGGTCAACGGGCCCGGCGAAGCCAAGGAAAGCGACATCGGTCTG
ACCGGCGGCGATCCGGCCAACCTCGTCTACATCGACGGCAAGCCGGCCAGCAAGCTGCGCAACGACCACCTGGTCGACGA
CCTGGAAGCACTGATCCGCGACAAGGTCCGCGAAAAAGAAGCCCGCGAACGCGATACCATCGCGCGCGAGGCATGA

Upstream 100 bases:

>100_bases
ACCGGCGTCGAAATCAGCTATCGAGACGACAACGTCGACTTGAGCGAGCATACCGGCAGCAACAACGTCGCCCGCTTCAC
CTTGGGAGAGTGATACGCCC

Downstream 100 bases:

>100_bases
CGCGCGCCGCTCGCGCCCCGTAACCGGTTCAGGAGTCAGCATTGAGCAACAAGATCCAGGCCATCCGTGGCATGAACGAT
CTGCTACCGGAGCAGTCGCC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MHMQSPIQRRKSRQIHVGHVPVGGDAPIAVQSMTNTDTLDVDATVAQIRQLEAAGADIVRVSVPTMDAAEAFGQIKRQVS
VPLVADIHFDYKIALRVAELGVDCLRINPGNIGREDRVRAVVDAARHHGIPIRIGVNAGSLEKDLQKKYGEPTPEALVES
AMRHIEHLDRLDFQDFKVSVKASDVFMAVAAYRQLAQRIDQPLHLGITEAGGLRSGTVKSSIGLGLLLMEGIGDTIRVSL
AADPVEEIKVGYDMLKSLRLRSKGINFIACPSCSRQNFDVIGTMNALEERLDDIMTPLDVSVIGCVVNGPGEAKESDIGL
TGGDPANLVYIDGKPASKLRNDHLVDDLEALIRDKVREKEARERDTIAREA

Sequences:

>Translated_371_residues
MHMQSPIQRRKSRQIHVGHVPVGGDAPIAVQSMTNTDTLDVDATVAQIRQLEAAGADIVRVSVPTMDAAEAFGQIKRQVS
VPLVADIHFDYKIALRVAELGVDCLRINPGNIGREDRVRAVVDAARHHGIPIRIGVNAGSLEKDLQKKYGEPTPEALVES
AMRHIEHLDRLDFQDFKVSVKASDVFMAVAAYRQLAQRIDQPLHLGITEAGGLRSGTVKSSIGLGLLLMEGIGDTIRVSL
AADPVEEIKVGYDMLKSLRLRSKGINFIACPSCSRQNFDVIGTMNALEERLDDIMTPLDVSVIGCVVNGPGEAKESDIGL
TGGDPANLVYIDGKPASKLRNDHLVDDLEALIRDKVREKEARERDTIAREA
>Mature_371_residues
MHMQSPIQRRKSRQIHVGHVPVGGDAPIAVQSMTNTDTLDVDATVAQIRQLEAAGADIVRVSVPTMDAAEAFGQIKRQVS
VPLVADIHFDYKIALRVAELGVDCLRINPGNIGREDRVRAVVDAARHHGIPIRIGVNAGSLEKDLQKKYGEPTPEALVES
AMRHIEHLDRLDFQDFKVSVKASDVFMAVAAYRQLAQRIDQPLHLGITEAGGLRSGTVKSSIGLGLLLMEGIGDTIRVSL
AADPVEEIKVGYDMLKSLRLRSKGINFIACPSCSRQNFDVIGTMNALEERLDDIMTPLDVSVIGCVVNGPGEAKESDIGL
TGGDPANLVYIDGKPASKLRNDHLVDDLEALIRDKVREKEARERDTIAREA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=366, Percent_Identity=74.0437158469945, Blast_Score=551, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_CHRSD (Q1QTK8)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_574899.1
- ProteinModelPortal:   Q1QTK8
- STRING:   Q1QTK8
- GeneID:   4028656
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_2854
- NMPDR:   fig|290398.4.peg.2438
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   INEDTMD
- ProtClustDB:   PRK00366
- BioCyc:   CSAL290398:CSAL_2854-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40380; Mature: 40380

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMQSPIQRRKSRQIHVGHVPVGGDAPIAVQSMTNTDTLDVDATVAQIRQLEAAGADIVR
CCCCCHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFGQIKRQVSVPLVADIHFDYKIALRVAELGVDCLRINPGNIGREDRVRA
EECCCCHHHHHHHHHHHHHCCCEEEEEECCHHEEEEHHHCCCEEEEECCCCCCHHHHHHH
VVDAARHHGIPIRIGVNAGSLEKDLQKKYGEPTPEALVESAMRHIEHLDRLDFQDFKVSV
HHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
KASDVFMAVAAYRQLAQRIDQPLHLGITEAGGLRSGTVKSSIGLGLLLMEGIGDTIRVSL
EHHHHHHHHHHHHHHHHHCCCCEEECEECCCCCCCCCCHHHCCHHHHHHHCCCCEEEEEE
AADPVEEIKVGYDMLKSLRLRSKGINFIACPSCSRQNFDVIGTMNALEERLDDIMTPLDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEECHHHHHHHHHHHCCCCCE
SVIGCVVNGPGEAKESDIGLTGGDPANLVYIDGKPASKLRNDHLVDDLEALIRDKVREKE
EEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHH
ARERDTIAREA
HHHHHHHHCCC
>Mature Secondary Structure
MHMQSPIQRRKSRQIHVGHVPVGGDAPIAVQSMTNTDTLDVDATVAQIRQLEAAGADIVR
CCCCCHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFGQIKRQVSVPLVADIHFDYKIALRVAELGVDCLRINPGNIGREDRVRA
EECCCCHHHHHHHHHHHHHCCCEEEEEECCHHEEEEHHHCCCEEEEECCCCCCHHHHHHH
VVDAARHHGIPIRIGVNAGSLEKDLQKKYGEPTPEALVESAMRHIEHLDRLDFQDFKVSV
HHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
KASDVFMAVAAYRQLAQRIDQPLHLGITEAGGLRSGTVKSSIGLGLLLMEGIGDTIRVSL
EHHHHHHHHHHHHHHHHHCCCCEEECEECCCCCCCCCCHHHCCHHHHHHHCCCCEEEEEE
AADPVEEIKVGYDMLKSLRLRSKGINFIACPSCSRQNFDVIGTMNALEERLDDIMTPLDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEECHHHHHHHHHHHCCCCCE
SVIGCVVNGPGEAKESDIGLTGGDPANLVYIDGKPASKLRNDHLVDDLEALIRDKVREKE
EEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHH
ARERDTIAREA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA