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The map label for this gene is rlmN

Identifier: 92114968

GI number: 92114968

Start: 3181478

End: 3182605

Strand: Direct

Name: rlmN

Synonym: Csal_2851

Alternate gene names: 92114968

Gene position: 3181478-3182605 (Clockwise)

Preceding gene: 92114967

Following gene: 92114969

Centisome position: 86.06

GC content: 62.77

Gene sequence:

>1128_bases
ATGACCCTCGATACCGTCACGCCCCGTACCAACCTTCTCGGCCTTACCCGCGAGGAGATGGAGTCCTTCTTCGTTTCGCT
GGGCGAGAAGAAATTCCGCGCCGCGCAGGTGATGAAGTGGATCCATCACGAAGGCTGCGCCGACTTCGCGTCGATGACCA
ACCTTTCGAAGGCGCTGCGCACGCGTCTCGAGGAGCTCGCCGAAATACGCGGGCCACGTGTGGTCTACGAGGGCACCTCG
CAGGACGGTACCCGCAAGTGGGTGCTCGAGGTCGAGGACGGCAGCTACGTGGAAACCGTGCTGATCCCGGCCGAGGGTGG
CAAGCGTCGGACGCTGTGCGTTTCCTCCCAGGTCGGCTGCTCGCTGGACTGCAGCTTCTGCTCCACCGGCAAGCAGGGGT
TTCAGCGCAACCTCACCAGCGCCGAGATCATCGGCCAGGTGTGGGTGGCCAGCAACAGCTTCGGGGCACGCCGGGACACC
ACCAATCGTCCGGTCACCAACGTGGTGATGATGGGCATGGGCGAGCCGCTACTCAATTACGACAACGTCGTGCCGGCCAT
GAAGCTGATGCTCGACGATAACGGTTACGGACTTTCCAAGCGCCGCGTCACGCTGTCGACCTCCGGCGTGGTGCCGAAAC
TCGACCAGCTCGGCGACGAGCTCGACGTGAGCCTGGCAGTGTCGCTGCATGCCGCCAACGACGAGCTGCGCAACGAACTG
GTGCCGTTGAACCGCAAGTACAACATCGCCACCTTGCTCGACGCCTGCCGTCGCTATCTCGCCAAGTGCGACGACACGCG
CATGCTGACGATCGAGTACACCCTCATCAAGGACGTCAACGATCAGCAGCATCATGCCGAGGAGCTCGCGGCCCTGCTCG
CCGACCTCCCCAGCAAGATCAACCTGATCCCTTTCAATCCGTTTCCCCACTCGGGCTACGAAAAGCCTTCGCGCAATCAG
GTCATGCGCTTTCAGCAATGGCTGTACGATCTCGGCTACACGGCCCTCGTACGCACCACGCGCGGCGACGACATCGACGC
CGCTTGCGGCCAGCTGGTGGGACGCGTGAAGGATCGCACCAAGCGGCACGAGCGTTACATCCAGTCCATCCAGCTCGACG
CTGATTGA

Upstream 100 bases:

>100_bases
TGCTTACTTCTTCGGCAACGAAGAGATCTGCGCACGCTGATCGCTTCGCGCGGGGGCCGCGAGGCCCCCGCACCGCCATC
CACCGAGCGTACGACCCACC

Downstream 100 bases:

>100_bases
GCCTTGCCTTGACTTCGCCAGGGTGCGGCGCTTTGATGGGACACTTCCAGACGACGAAATCCATCGCCTCGCACCGTTTG
ACGCCATTGTTTCGAGAGGG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTLDTVTPRTNLLGLTREEMESFFVSLGEKKFRAAQVMKWIHHEGCADFASMTNLSKALRTRLEELAEIRGPRVVYEGTS
QDGTRKWVLEVEDGSYVETVLIPAEGGKRRTLCVSSQVGCSLDCSFCSTGKQGFQRNLTSAEIIGQVWVASNSFGARRDT
TNRPVTNVVMMGMGEPLLNYDNVVPAMKLMLDDNGYGLSKRRVTLSTSGVVPKLDQLGDELDVSLAVSLHAANDELRNEL
VPLNRKYNIATLLDACRRYLAKCDDTRMLTIEYTLIKDVNDQQHHAEELAALLADLPSKINLIPFNPFPHSGYEKPSRNQ
VMRFQQWLYDLGYTALVRTTRGDDIDAACGQLVGRVKDRTKRHERYIQSIQLDAD

Sequences:

>Translated_375_residues
MTLDTVTPRTNLLGLTREEMESFFVSLGEKKFRAAQVMKWIHHEGCADFASMTNLSKALRTRLEELAEIRGPRVVYEGTS
QDGTRKWVLEVEDGSYVETVLIPAEGGKRRTLCVSSQVGCSLDCSFCSTGKQGFQRNLTSAEIIGQVWVASNSFGARRDT
TNRPVTNVVMMGMGEPLLNYDNVVPAMKLMLDDNGYGLSKRRVTLSTSGVVPKLDQLGDELDVSLAVSLHAANDELRNEL
VPLNRKYNIATLLDACRRYLAKCDDTRMLTIEYTLIKDVNDQQHHAEELAALLADLPSKINLIPFNPFPHSGYEKPSRNQ
VMRFQQWLYDLGYTALVRTTRGDDIDAACGQLVGRVKDRTKRHERYIQSIQLDAD
>Mature_374_residues
TLDTVTPRTNLLGLTREEMESFFVSLGEKKFRAAQVMKWIHHEGCADFASMTNLSKALRTRLEELAEIRGPRVVYEGTSQ
DGTRKWVLEVEDGSYVETVLIPAEGGKRRTLCVSSQVGCSLDCSFCSTGKQGFQRNLTSAEIIGQVWVASNSFGARRDTT
NRPVTNVVMMGMGEPLLNYDNVVPAMKLMLDDNGYGLSKRRVTLSTSGVVPKLDQLGDELDVSLAVSLHAANDELRNELV
PLNRKYNIATLLDACRRYLAKCDDTRMLTIEYTLIKDVNDQQHHAEELAALLADLPSKINLIPFNPFPHSGYEKPSRNQV
MRFQQWLYDLGYTALVRTTRGDDIDAACGQLVGRVKDRTKRHERYIQSIQLDAD

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=358, Percent_Identity=60.6145251396648, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_CHRSD (Q1QTL1)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_574896.1
- ProteinModelPortal:   Q1QTL1
- SMR:   Q1QTL1
- STRING:   Q1QTL1
- GeneID:   4028653
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_2851
- NMPDR:   fig|290398.4.peg.2435
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- BioCyc:   CSAL290398:CSAL_2851-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 42100; Mature: 41968

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDTVTPRTNLLGLTREEMESFFVSLGEKKFRAAQVMKWIHHEGCADFASMTNLSKALR
CCCCCCCCCHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TRLEELAEIRGPRVVYEGTSQDGTRKWVLEVEDGSYVETVLIPAEGGKRRTLCVSSQVGC
HHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEEEECCCCC
SLDCSFCSTGKQGFQRNLTSAEIIGQVWVASNSFGARRDTTNRPVTNVVMMGMGEPLLNY
CEECHHHHCCHHHHHHCCCHHHHHHHHEEECCCCCCCCCCCCCCHHHHHEECCCCCCCCC
DNVVPAMKLMLDDNGYGLSKRRVTLSTSGVVPKLDQLGDELDVSLAVSLHAANDELRNEL
CHHHHHHHEEECCCCCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEEEEECCCHHHHHHC
VPLNRKYNIATLLDACRRYLAKCDDTRMLTIEYTLIKDVNDQQHHAEELAALLADLPSKI
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCC
NLIPFNPFPHSGYEKPSRNQVMRFQQWLYDLGYTALVRTTRGDDIDAACGQLVGRVKDRT
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
KRHERYIQSIQLDAD
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
TLDTVTPRTNLLGLTREEMESFFVSLGEKKFRAAQVMKWIHHEGCADFASMTNLSKALR
CCCCCCCCHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TRLEELAEIRGPRVVYEGTSQDGTRKWVLEVEDGSYVETVLIPAEGGKRRTLCVSSQVGC
HHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEEEECCCCC
SLDCSFCSTGKQGFQRNLTSAEIIGQVWVASNSFGARRDTTNRPVTNVVMMGMGEPLLNY
CEECHHHHCCHHHHHHCCCHHHHHHHHEEECCCCCCCCCCCCCCHHHHHEECCCCCCCCC
DNVVPAMKLMLDDNGYGLSKRRVTLSTSGVVPKLDQLGDELDVSLAVSLHAANDELRNEL
CHHHHHHHEEECCCCCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEEEEECCCHHHHHHC
VPLNRKYNIATLLDACRRYLAKCDDTRMLTIEYTLIKDVNDQQHHAEELAALLADLPSKI
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCC
NLIPFNPFPHSGYEKPSRNQVMRFQQWLYDLGYTALVRTTRGDDIDAACGQLVGRVKDRT
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
KRHERYIQSIQLDAD
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA