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The map label for this gene is trmJ [H]

Identifier: 92114963

GI number: 92114963

Start: 3177846

End: 3178670

Strand: Direct

Name: trmJ [H]

Synonym: Csal_2846

Alternate gene names: 92114963

Gene position: 3177846-3178670 (Clockwise)

Preceding gene: 92114961

Following gene: 92114964

Centisome position: 85.97

GC content: 67.27

Gene sequence:

>825_bases
ATGATAGACTCGCCGTTCGTTCCTCAGCCCCGCGCCTTATTCATCATGCTCGACAAACTTCGTATCGTCCTCATCGGCAC
CAGCCACCCCGGCAACATCGGCGGCACCGCCCGTGCCATGCACAACATGGGGCTCGCCGACCTGGCCCTCGTCGCGCCGC
GCTGCGAGGTGTACGGCTCGGAATCCCACGCGCGTGCCTCCGGCGCCGATGCGCTGGTCAGCAACGCCCGCGTGCATGCC
ACCCTCGAGGAAGCCGTCAGCGACTGCACCTTCGTGGTCGGCGCCAGCGCCCGTGTGCGCCACCTGCCCTGGCCCATGCT
CACGCCCCGCGCGCTCGGCGAGCGTCTGCCCCGGGAGTGCGCGCCGGAGAACGCACGGGCCGCGCTGGTCTTCGGCCGCG
AGGACAGCGGCATGACCAACGGCGAGCTGCAACGCTGTCATGCGCATGTGCACATCCCCACCAACCCCGACTTCAGCTCG
CTCAACCTCGCCGCAGCGGTCCAGGTGCTCGCCTACGAGTGCCGTCTGGCGTTTCTGGGCAGCGACGCGGAAGATACCGA
GACGCCCTTCGGCGTCGAATGGGACAACCCCCCGGCCACGCACGACGACCTGGAGCGTTTCTTCGAGCATCTGGAGCGCA
CCCTGATCGATATCGACTTCCACGATCCGGACAACCCGCGCCAGCTGATGGCACGCCTGCGGCGGCTCTTCCTGCGCACG
CATATCGACAGCATGGAAATGAACATCCTGCGCGGCATCCTCGGTGCCGTGAACAAGCAGAGCCGCGCTCTGGCGGCACG
CAAAGACGGCGACGGGAATGGTTGA

Upstream 100 bases:

>100_bases
TGAATCTCGGAAACTGTTAAAGAACGTGGCGCATCCTAGCAGACGGGGCCCTGCACGGAAAGCGCCGCAGCCCGCAGCCC
GCAGCCCGCAGCCCGCAGCC

Downstream 100 bases:

>100_bases
CCGCGTTACTGGGTTTTCCCATAATGCGATCACGCTTCAGGCGACGGGTCCCCCACGGATCGCATGCTCCGCCTGGTCAA
CCGCTCTTTCACCGCTGCCC

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MIDSPFVPQPRALFIMLDKLRIVLIGTSHPGNIGGTARAMHNMGLADLALVAPRCEVYGSESHARASGADALVSNARVHA
TLEEAVSDCTFVVGASARVRHLPWPMLTPRALGERLPRECAPENARAALVFGREDSGMTNGELQRCHAHVHIPTNPDFSS
LNLAAAVQVLAYECRLAFLGSDAEDTETPFGVEWDNPPATHDDLERFFEHLERTLIDIDFHDPDNPRQLMARLRRLFLRT
HIDSMEMNILRGILGAVNKQSRALAARKDGDGNG

Sequences:

>Translated_274_residues
MIDSPFVPQPRALFIMLDKLRIVLIGTSHPGNIGGTARAMHNMGLADLALVAPRCEVYGSESHARASGADALVSNARVHA
TLEEAVSDCTFVVGASARVRHLPWPMLTPRALGERLPRECAPENARAALVFGREDSGMTNGELQRCHAHVHIPTNPDFSS
LNLAAAVQVLAYECRLAFLGSDAEDTETPFGVEWDNPPATHDDLERFFEHLERTLIDIDFHDPDNPRQLMARLRRLFLRT
HIDSMEMNILRGILGAVNKQSRALAARKDGDGNG
>Mature_274_residues
MIDSPFVPQPRALFIMLDKLRIVLIGTSHPGNIGGTARAMHNMGLADLALVAPRCEVYGSESHARASGADALVSNARVHA
TLEEAVSDCTFVVGASARVRHLPWPMLTPRALGERLPRECAPENARAALVFGREDSGMTNGELQRCHAHVHIPTNPDFSS
LNLAAAVQVLAYECRLAFLGSDAEDTETPFGVEWDNPPATHDDLERFFEHLERTLIDIDFHDPDNPRQLMARLRRLFLRT
HIDSMEMNILRGILGAVNKQSRALAARKDGDGNG

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=252, Percent_Identity=50, Blast_Score=231, Evalue=3e-62,
Organism=Escherichia coli, GI1790865, Length=156, Percent_Identity=36.5384615384615, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30098; Mature: 30098

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSPFVPQPRALFIMLDKLRIVLIGTSHPGNIGGTARAMHNMGLADLALVAPRCEVYGS
CCCCCCCCCCCEEEEEEECEEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHCCHHHCCCC
ESHARASGADALVSNARVHATLEEAVSDCTFVVGASARVRHLPWPMLTPRALGERLPREC
CCCCCCCCHHHHHHCCHHHHHHHHHHHHCEEEECCCCCEEECCCCCCCHHHHHHHCCHHC
APENARAALVFGREDSGMTNGELQRCHAHVHIPTNPDFSSLNLAAAVQVLAYECRLAFLG
CCCCCCEEEEEECCCCCCCCCHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEC
SDAEDTETPFGVEWDNPPATHDDLERFFEHLERTLIDIDFHDPDNPRQLMARLRRLFLRT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHH
HIDSMEMNILRGILGAVNKQSRALAARKDGDGNG
HHCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIDSPFVPQPRALFIMLDKLRIVLIGTSHPGNIGGTARAMHNMGLADLALVAPRCEVYGS
CCCCCCCCCCCEEEEEEECEEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHCCHHHCCCC
ESHARASGADALVSNARVHATLEEAVSDCTFVVGASARVRHLPWPMLTPRALGERLPREC
CCCCCCCCHHHHHHCCHHHHHHHHHHHHCEEEECCCCCEEECCCCCCCHHHHHHHCCHHC
APENARAALVFGREDSGMTNGELQRCHAHVHIPTNPDFSSLNLAAAVQVLAYECRLAFLG
CCCCCCEEEEEECCCCCCCCCHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEC
SDAEDTETPFGVEWDNPPATHDDLERFFEHLERTLIDIDFHDPDNPRQLMARLRRLFLRT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHH
HIDSMEMNILRGILGAVNKQSRALAARKDGDGNG
HHCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA