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The map label for this gene is ogt [H]

Identifier: 92114913

GI number: 92114913

Start: 3126603

End: 3127151

Strand: Direct

Name: ogt [H]

Synonym: Csal_2796

Alternate gene names: 92114913

Gene position: 3126603-3127151 (Clockwise)

Preceding gene: 92114912

Following gene: 92114914

Centisome position: 84.58

GC content: 66.85

Gene sequence:

>549_bases
ATGTCGTCCCTCGAGTCCGCCATCGACTACGTCGTCACGCCCGTACCGGCCTCGCCGCTGGGCACGATACGCATCCGCGC
CAGTGCACGTGGCATTCGCGAAATCGCGTTCGTGGAGGCGCCCGACGAGACACCGCACCCTTCAGCGCTCACCGATGCCT
GCCGCGTGCAACTGAGCGACTATCTCCAGGGCGAGCGTCGCGAGTTCGACCTCCCGCTCGACCCTGTCGGTACTCCGTTT
CAACGACGGGTCTGGGCAGCCCTGGCAGAGATTCCCCATGGGCAGACACGCAGTTACGCGGACATCGCCCGGCACATCGG
CAATCTCAAGGCCATGCGGGCGGTCGGCGCCGCCAATGGCCGCAACCCGATCGCCATCGTCGTGCCCTGCCATCGGGTCA
TCGGCCGCAATGGCCAACTCACCGGTTACGCCGGCGGGCTCGAGCGCAAGGCCTGGTTGCTGGCGCTCGAGCGTCACGGT
CATGCAGACAGCGGGGCGCTCGCCGGCGTCCCCGGGCAAGGGGATCATCATCAGGATCATTGGAGGTAA

Upstream 100 bases:

>100_bases
CTGGGCGTCAAGCGAGCGCTGTCGGCGCTGGGTACGGTCGAGCCGGCCCATGCCACGCCATGGCGCAGTTACCTCACCCT
GCAACTCTGGAGCCTTTGAC

Downstream 100 bases:

>100_bases
GCCATGACGCAACAGCACGAAGACGCCCGGCAGGTACTGGATTTCTGGTTCGAAGAGGCGGGGCCCAAGCAGTGGTTCAG
CAAGAGCGCCTCGTTCGATC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MSSLESAIDYVVTPVPASPLGTIRIRASARGIREIAFVEAPDETPHPSALTDACRVQLSDYLQGERREFDLPLDPVGTPF
QRRVWAALAEIPHGQTRSYADIARHIGNLKAMRAVGAANGRNPIAIVVPCHRVIGRNGQLTGYAGGLERKAWLLALERHG
HADSGALAGVPGQGDHHQDHWR

Sequences:

>Translated_182_residues
MSSLESAIDYVVTPVPASPLGTIRIRASARGIREIAFVEAPDETPHPSALTDACRVQLSDYLQGERREFDLPLDPVGTPF
QRRVWAALAEIPHGQTRSYADIARHIGNLKAMRAVGAANGRNPIAIVVPCHRVIGRNGQLTGYAGGLERKAWLLALERHG
HADSGALAGVPGQGDHHQDHWR
>Mature_181_residues
SSLESAIDYVVTPVPASPLGTIRIRASARGIREIAFVEAPDETPHPSALTDACRVQLSDYLQGERREFDLPLDPVGTPFQ
RRVWAALAEIPHGQTRSYADIARHIGNLKAMRAVGAANGRNPIAIVVPCHRVIGRNGQLTGYAGGLERKAWLLALERHGH
ADSGALAGVPGQGDHHQDHWR

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=161, Percent_Identity=34.7826086956522, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1787596, Length=110, Percent_Identity=55.4545454545455, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=51.685393258427, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=53.75, Blast_Score=90, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=53.75, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=45.8823529411765, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19727; Mature: 19595

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLESAIDYVVTPVPASPLGTIRIRASARGIREIAFVEAPDETPHPSALTDACRVQLSD
CCHHHHHHHHEECCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
YLQGERREFDLPLDPVGTPFQRRVWAALAEIPHGQTRSYADIARHIGNLKAMRAVGAANG
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
RNPIAIVVPCHRVIGRNGQLTGYAGGLERKAWLLALERHGHADSGALAGVPGQGDHHQDH
CCCEEEEEECHHHHCCCCCEEEECCCCCHHHHEEEHHHCCCCCCCCEECCCCCCCCCCCC
WR
CC
>Mature Secondary Structure 
SSLESAIDYVVTPVPASPLGTIRIRASARGIREIAFVEAPDETPHPSALTDACRVQLSD
CHHHHHHHHEECCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
YLQGERREFDLPLDPVGTPFQRRVWAALAEIPHGQTRSYADIARHIGNLKAMRAVGAANG
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
RNPIAIVVPCHRVIGRNGQLTGYAGGLERKAWLLALERHGHADSGALAGVPGQGDHHQDH
CCCEEEEEECHHHHCCCCCEEEECCCCCHHHHEEEHHHCCCCCCCCEECCCCCCCCCCCC
WR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]