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The map label for this gene is argT [H]

Identifier: 92114902

GI number: 92114902

Start: 3115964

End: 3116803

Strand: Direct

Name: argT [H]

Synonym: Csal_2784

Alternate gene names: 92114902

Gene position: 3115964-3116803 (Clockwise)

Preceding gene: 92114901

Following gene: 92114903

Centisome position: 84.29

GC content: 60.48

Gene sequence:

>840_bases
TTGAAGCATTGCGCTTCACGACAACACGACGACCGCGAGATTGTTGACATGAAAAAGCTGCTCACGCTGACCCTGCTGGG
AATGTTCCTGGCGACCGGCACCGCCGAGGCACGGGGTACCGACGAGGTGCGCCTGGCCATCGACGTGCCTTACGAGCCCT
TTGTGTATCGCACCCCGGAAGGAGAACTGGCGGGCTTCGAGATCGACCTCGGCAACGAGCTGTGTGCCCGCGCCGATCTC
GAATGCACCTGGATCGAGCAATCCTGGGACGGCATCATCCCCGGGCTTCTCTCGCGCAAATACGACGCCATTCTCTCTTC
GATGGCCATCACCCCCGAGCGCGAAAACCAGGTGCTGTTTTCGATTCCCTACTACAACACCCCCAGTGTCTGGATCACGC
GGCGTGACCGCGACATCGACATCAGCGACAAGGCCTCGCTCGAGGGGCTTTCCGTCGGCGTGCAACGCGGCACCATCCGC
GACAGCTACGTCACGCAGATGTACGGCGACACCCTCGACATTCGCCGTTACTCGTCATCGCAGGATGTCGCCAACGACCT
CGTCGCCGGGCGTCTCGATCTCTCGTTCGAGGACTATCCCCTGGCCGTGGACACCTTCCACTTCAATGAAGCGGACAGCG
AGTTCAAGCAGATTGGTGACAGCATCAAGAAACCCGTTTCCATTTTCGGCAAGGGCGCGGCGATGGCCTTTCGCAAACGC
GACGAGGCCCTCGCCGAGAAGTTCAATACGGCGCTGCGCGACGTCTATGCCGATGGCACCTTCGATGAGCTGATGCACCA
GTACTTCGACTACGACCTGTCACTCCCCGTCGGCGAATGA

Upstream 100 bases:

>100_bases
TGTTCGTGGCGGCGATCTACCTGTGCTTGACCTTCGCCATCCTGTATCTCTTCCGCTACCTGGAAAAGAAACTCCTGGCG
CACCTCAAGCCGATCAACGG

Downstream 100 bases:

>100_bases
TATCCGATGGCCGGCGGCTTCGGCCGCCGGCACTTTCAAGGAAAGATCATGCGACACCACACGCATCCCTTGCTGAGCCC
CGTTCCCGGCACCCAGCGCG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKHCASRQHDDREIVDMKKLLTLTLLGMFLATGTAEARGTDEVRLAIDVPYEPFVYRTPEGELAGFEIDLGNELCARADL
ECTWIEQSWDGIIPGLLSRKYDAILSSMAITPERENQVLFSIPYYNTPSVWITRRDRDIDISDKASLEGLSVGVQRGTIR
DSYVTQMYGDTLDIRRYSSSQDVANDLVAGRLDLSFEDYPLAVDTFHFNEADSEFKQIGDSIKKPVSIFGKGAAMAFRKR
DEALAEKFNTALRDVYADGTFDELMHQYFDYDLSLPVGE

Sequences:

>Translated_279_residues
MKHCASRQHDDREIVDMKKLLTLTLLGMFLATGTAEARGTDEVRLAIDVPYEPFVYRTPEGELAGFEIDLGNELCARADL
ECTWIEQSWDGIIPGLLSRKYDAILSSMAITPERENQVLFSIPYYNTPSVWITRRDRDIDISDKASLEGLSVGVQRGTIR
DSYVTQMYGDTLDIRRYSSSQDVANDLVAGRLDLSFEDYPLAVDTFHFNEADSEFKQIGDSIKKPVSIFGKGAAMAFRKR
DEALAEKFNTALRDVYADGTFDELMHQYFDYDLSLPVGE
>Mature_279_residues
MKHCASRQHDDREIVDMKKLLTLTLLGMFLATGTAEARGTDEVRLAIDVPYEPFVYRTPEGELAGFEIDLGNELCARADL
ECTWIEQSWDGIIPGLLSRKYDAILSSMAITPERENQVLFSIPYYNTPSVWITRRDRDIDISDKASLEGLSVGVQRGTIR
DSYVTQMYGDTLDIRRYSSSQDVANDLVAGRLDLSFEDYPLAVDTFHFNEADSEFKQIGDSIKKPVSIFGKGAAMAFRKR
DEALAEKFNTALRDVYADGTFDELMHQYFDYDLSLPVGE

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=34.6303501945525, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=35.3448275862069, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787085, Length=255, Percent_Identity=34.9019607843137, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787088, Length=253, Percent_Identity=31.6205533596838, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788228, Length=238, Percent_Identity=26.0504201680672, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=25.296442687747, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31532; Mature: 31532

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHCASRQHDDREIVDMKKLLTLTLLGMFLATGTAEARGTDEVRLAIDVPYEPFVYRTPE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEECCC
GELAGFEIDLGNELCARADLECTWIEQSWDGIIPGLLSRKYDAILSSMAITPERENQVLF
CCEEEEEEECCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEE
SIPYYNTPSVWITRRDRDIDISDKASLEGLSVGVQRGTIRDSYVTQMYGDTLDIRRYSSS
EECCCCCCCEEEEECCCCCCCCCCCCCCHHEEEHCCCCCCHHHHHHHHCCCEEEEECCCC
QDVANDLVAGRLDLSFEDYPLAVDTFHFNEADSEFKQIGDSIKKPVSIFGKGAAMAFRKR
HHHHHHHHHCEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHH
DEALAEKFNTALRDVYADGTFDELMHQYFDYDLSLPVGE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKHCASRQHDDREIVDMKKLLTLTLLGMFLATGTAEARGTDEVRLAIDVPYEPFVYRTPE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEECCC
GELAGFEIDLGNELCARADLECTWIEQSWDGIIPGLLSRKYDAILSSMAITPERENQVLF
CCEEEEEEECCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEE
SIPYYNTPSVWITRRDRDIDISDKASLEGLSVGVQRGTIRDSYVTQMYGDTLDIRRYSSS
EECCCCCCCEEEEECCCCCCCCCCCCCCHHEEEHCCCCCCHHHHHHHHCCCEEEEECCCC
QDVANDLVAGRLDLSFEDYPLAVDTFHFNEADSEFKQIGDSIKKPVSIFGKGAAMAFRKR
HHHHHHHHHCEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHH
DEALAEKFNTALRDVYADGTFDELMHQYFDYDLSLPVGE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]