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The map label for this gene is greB [H]

Identifier: 92114676

GI number: 92114676

Start: 2869852

End: 2870388

Strand: Direct

Name: greB [H]

Synonym: Csal_2558

Alternate gene names: 92114676

Gene position: 2869852-2870388 (Clockwise)

Preceding gene: 92114667

Following gene: 92114679

Centisome position: 77.63

GC content: 62.76

Gene sequence:

>537_bases
ATGAAAGGCCGCAACATGACTCGCTGGCGCGATCCGGCCAAGGATCCACGCCAGGAAAAGAAAAGCTCATTGATCACGGC
ACGGGGCTATGCCCGGCTCAAGGGCATCCACGAGCACCTTTCCCGCGTCAAGCGTCCCGCCCTCTCCACCAAGGTCGGTG
AGGCGGCCGCGCTCGGCGACCGTAGCGAAAACGCCGACTACACCTACAACAAGAAGGAACTCAATCGCGTCATTGCGCGT
ATTCGCTACCTGGGCAAGCGCCTCGACGAACTCCAGGTCGTCGATCGCCTGCCCGCGGATACCTCACGCGTCTATTTCGG
CGCCTACGTGACGCTGGAGGATGACGACGGCGAGGAAATGACGATCCGCATCGTCGGTCACGACGAAACCGATACCGACA
AGCGCTGGATCAGTGTCGACGCCCCGCTCGCCCAAGCCATGCTCGGCAAGGGACTCGACGAGGACGTCACCGTCAACGCG
CCGGGCGGCGACACCACCTACATCATCACGGACATCGCCTACGACCCCGAGGCCTGA

Upstream 100 bases:

>100_bases
ACGTCGATTCGTCGTAGTGGCCACGTTCATAGCCCGACATCGGGCTTGGCGTACCGACGCGGCTTGCGTAGGGTAAGCAC
TCCACGCTCTGGAGGTAGGC

Downstream 100 bases:

>100_bases
GCCGGGCCTGGTGCAGCGTCAGCGTCTCGTCTTCACCGCCTGGTAGACCCGAAAACGCCGATCGTCGGCCAGCACCGTGA
ACTCGCCGAAGGCGTCTTCC

Product: GreA/GreB family elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MKGRNMTRWRDPAKDPRQEKKSSLITARGYARLKGIHEHLSRVKRPALSTKVGEAAALGDRSENADYTYNKKELNRVIAR
IRYLGKRLDELQVVDRLPADTSRVYFGAYVTLEDDDGEEMTIRIVGHDETDTDKRWISVDAPLAQAMLGKGLDEDVTVNA
PGGDTTYIITDIAYDPEA

Sequences:

>Translated_178_residues
MKGRNMTRWRDPAKDPRQEKKSSLITARGYARLKGIHEHLSRVKRPALSTKVGEAAALGDRSENADYTYNKKELNRVIAR
IRYLGKRLDELQVVDRLPADTSRVYFGAYVTLEDDDGEEMTIRIVGHDETDTDKRWISVDAPLAQAMLGKGLDEDVTVNA
PGGDTTYIITDIAYDPEA
>Mature_178_residues
MKGRNMTRWRDPAKDPRQEKKSSLITARGYARLKGIHEHLSRVKRPALSTKVGEAAALGDRSENADYTYNKKELNRVIAR
IRYLGKRLDELQVVDRLPADTSRVYFGAYVTLEDDDGEEMTIRIVGHDETDTDKRWISVDAPLAQAMLGKGLDEDVTVNA
PGGDTTYIITDIAYDPEA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=45.4545454545455, Blast_Score=140, Evalue=3e-35,
Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=35.9477124183007, Blast_Score=93, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20024; Mature: 20024

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGRNMTRWRDPAKDPRQEKKSSLITARGYARLKGIHEHLSRVKRPALSTKVGEAAALGD
CCCCCCCCCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHCCC
RSENADYTYNKKELNRVIARIRYLGKRLDELQVVDRLPADTSRVYFGAYVTLEDDDGEEM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEE
TIRIVGHDETDTDKRWISVDAPLAQAMLGKGLDEDVTVNAPGGDTTYIITDIAYDPEA
EEEEEECCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MKGRNMTRWRDPAKDPRQEKKSSLITARGYARLKGIHEHLSRVKRPALSTKVGEAAALGD
CCCCCCCCCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHCCC
RSENADYTYNKKELNRVIARIRYLGKRLDELQVVDRLPADTSRVYFGAYVTLEDDDGEEM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCEE
TIRIVGHDETDTDKRWISVDAPLAQAMLGKGLDEDVTVNAPGGDTTYIITDIAYDPEA
EEEEEECCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]