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The map label for this gene is nadE2 [H]

Identifier: 92114479

GI number: 92114479

Start: 2650333

End: 2651175

Strand: Direct

Name: nadE2 [H]

Synonym: Csal_2359

Alternate gene names: 92114479

Gene position: 2650333-2651175 (Clockwise)

Preceding gene: 92114478

Following gene: 92114482

Centisome position: 71.7

GC content: 67.5

Gene sequence:

>843_bases
ATGCACTATCAGGATCACCTGCGCGTCGGCGCCGCGCAGATCAACGCCAGCCTCGGCGATGTCGACGCCAACCTCGAACG
CCATCTCGCGTTCATTGCCGATGCACGCCGCAAGGGGCTCGAATTGCTGGTCTTCCCCGAGCTCTCGCTGACCGGCTACG
GCCTGGGCAACCGGGTGATCGACGTGGCCTGCCCGGCTCATGATCCGCGCCTGGCCGAGCTGGCCCGCGCCGCCGGCGAG
ATGCAGGTGGTGGTCGGCTTCGTCGAGGAGGCCAGCCCCGGCGAGTACTACAACGCCCTGGCGATTCTCCAGCACGGCAA
GCTTCAGGCCGTGCACCGCAAGCTCAACCTGCCCACCTACGGTGGCCTGGAAGAAGGCAAGCTGTTCACCCATGGCAGCG
AGCTCACGCATCTTCCGGTGTGTCCCGGCTGGTCGGCGACCTCGCTGATCTGCGCCGACCTGTGGAACCCGGGGTTGGTG
CACGCCGCCCTGCTGGCCCGCCCCACCGTGCTGTGCGCACCGATCAACTCGGCCTCGGGCATCGTCAGCGACGACTTCTC
CAACGAGCAGAACTGGGCCGTCAACCTGCAGTTCTACGCCATGACCTACGGCACCCCCGTAATCATGGCCAACCGCTTCG
GCCCCGAAGGCAACAGCCACTTCTGGGGCGGCTCGCGCATTCTCGGCCCGCGCGGCGACACCCTCGCCCTGGCCGAGGAC
GGCGAAACGCTGATCGAAGCCCAGCTCTCGCGCACCGCCATCGCCCGCGCCCGCTTCGAACTCCCCACCCACCGCGACGC
CGACACGCCACTGGTACGTGAATTGATGGACGGCTATCGCTGA

Upstream 100 bases:

>100_bases
CTCGGCGCGCCTTGAAGCAACGCCGCGCGGGTGGGATGATCCGCCTCGCCGGCGAGGCACGCGCCGGCATCTTTCAGACC
CAGCGTAGCGAGACGCCACC

Downstream 100 bases:

>100_bases
ACCCGGCCCCAAACGACACTCGGCGCCTCCCAAGGGAAGGCGCCGAGCGCATGTGAAGCGGGGTCTAAACCGTCAAAGAC
GAATCAGTCGCGGCGATACG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MHYQDHLRVGAAQINASLGDVDANLERHLAFIADARRKGLELLVFPELSLTGYGLGNRVIDVACPAHDPRLAELARAAGE
MQVVVGFVEEASPGEYYNALAILQHGKLQAVHRKLNLPTYGGLEEGKLFTHGSELTHLPVCPGWSATSLICADLWNPGLV
HAALLARPTVLCAPINSASGIVSDDFSNEQNWAVNLQFYAMTYGTPVIMANRFGPEGNSHFWGGSRILGPRGDTLALAED
GETLIEAQLSRTAIARARFELPTHRDADTPLVRELMDGYR

Sequences:

>Translated_280_residues
MHYQDHLRVGAAQINASLGDVDANLERHLAFIADARRKGLELLVFPELSLTGYGLGNRVIDVACPAHDPRLAELARAAGE
MQVVVGFVEEASPGEYYNALAILQHGKLQAVHRKLNLPTYGGLEEGKLFTHGSELTHLPVCPGWSATSLICADLWNPGLV
HAALLARPTVLCAPINSASGIVSDDFSNEQNWAVNLQFYAMTYGTPVIMANRFGPEGNSHFWGGSRILGPRGDTLALAED
GETLIEAQLSRTAIARARFELPTHRDADTPLVRELMDGYR
>Mature_280_residues
MHYQDHLRVGAAQINASLGDVDANLERHLAFIADARRKGLELLVFPELSLTGYGLGNRVIDVACPAHDPRLAELARAAGE
MQVVVGFVEEASPGEYYNALAILQHGKLQAVHRKLNLPTYGGLEEGKLFTHGSELTHLPVCPGWSATSLICADLWNPGLV
HAALLARPTVLCAPINSASGIVSDDFSNEQNWAVNLQFYAMTYGTPVIMANRFGPEGNSHFWGGSRILGPRGDTLALAED
GETLIEAQLSRTAIARARFELPTHRDADTPLVRELMDGYR

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 30431; Mature: 30431

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYQDHLRVGAAQINASLGDVDANLERHLAFIADARRKGLELLVFPELSLTGYGLGNRVI
CCCHHHHEECHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCEEE
DVACPAHDPRLAELARAAGEMQVVVGFVEEASPGEYYNALAILQHGKLQAVHRKLNLPTY
EEECCCCCCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCC
GGLEEGKLFTHGSELTHLPVCPGWSATSLICADLWNPGLVHAALLARPTVLCAPINSASG
CCCCCCEEEECCCCCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCCCCC
IVSDDFSNEQNWAVNLQFYAMTYGTPVIMANRFGPEGNSHFWGGSRILGPRGDTLALAED
CCCCCCCCCCCEEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEECCCCCEEEEECC
GETLIEAQLSRTAIARARFELPTHRDADTPLVRELMDGYR
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MHYQDHLRVGAAQINASLGDVDANLERHLAFIADARRKGLELLVFPELSLTGYGLGNRVI
CCCHHHHEECHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCEEE
DVACPAHDPRLAELARAAGEMQVVVGFVEEASPGEYYNALAILQHGKLQAVHRKLNLPTY
EEECCCCCCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCC
GGLEEGKLFTHGSELTHLPVCPGWSATSLICADLWNPGLVHAALLARPTVLCAPINSASG
CCCCCCEEEECCCCCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCCCCC
IVSDDFSNEQNWAVNLQFYAMTYGTPVIMANRFGPEGNSHFWGGSRILGPRGDTLALAED
CCCCCCCCCCCEEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEECCCCCEEEEECC
GETLIEAQLSRTAIARARFELPTHRDADTPLVRELMDGYR
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]