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The map label for this gene is purH [H]

Identifier: 92114410

GI number: 92114410

Start: 2577253

End: 2578845

Strand: Direct

Name: purH [H]

Synonym: Csal_2289

Alternate gene names: 92114410

Gene position: 2577253-2578845 (Clockwise)

Preceding gene: 92114409

Following gene: 92114421

Centisome position: 69.72

GC content: 66.1

Gene sequence:

>1593_bases
ATGGCCGAACACACTACCCCGTCGTCCGCCCGCCCCGTGCGCCGCGCATTGATCAGCGTGTCCGACAAGACCGGCATCGT
GGAGTTCGCCCGCGAGCTGGCCGCGCGTGGCGTCGCGCTGCTGTCCACCGGCGGCACGTTCCGCCTGCTCAGCGAGCACG
GTATCCCGGTGACGGAAGTCTCCCAACACACCGGTTTCCCGGAAATCATGGACGGCCGCGTCAAGACCCTGCACCCCAAG
ATTCACGGCGGCATTCTCGGTCGTCGCGGGCAGGACGACGAGGTGATGGCCGCGCATGACATCGATGCCATCGACATGGT
CGTGGTCAACCTCTACCCCTTCGCCGACACTGTGGCCCGCGAGGATTGCACGCTGGAAGAGGCCATCGAGAACATCGACA
TCGGCGGCCCGACCATGGTGCGCGCCTGTGCCAAGAACCACGCCCACACCACGATCGTGGTCAACGCCGGCGACTACTCG
CGCGTACTGGATGACATGAGCGCCCAGGGCGGCGCCGTCGGCCAGGCGCTGCGCTTCGACCTGGCGGTCAAGGCCTTCGA
GCACACCGCCGGTTACGACGGCGCCATCGCCAACTATCTCGGTACCCTGGCGGAAGGCGGTGAAGCCAACTTCCCGCGCA
CCTACAACGTGCAGTTCCACAAGAAGCAGGCCATGCGCTACGGCGAGAACCCGCACCAGCAGGCTGCGTTCTATGCCGAA
GCCGATGCCGCGGAAGCCAGCGTAACCACTGCCCGGCAACGCCAGGGCAAGGCGCTTTCCTTCAACAACGTCGCCGATAC
CGATGCTGCCTTCGAGTGCGTCAAGGCCTTCCGCGAGACGGCCTGCGTCATCGTCAAGCACGCCAACCCCTGTGGCGTCG
CCGTCGCCGAGACCCCGCTCGCGGCCTACGAGCGCGCTTTCGCCACCGATCCCACCAGTGCGTTCGGCGGCATCATCGCC
TTCAATCGTGCGCTCGATGCCGCCACGGCTCGCGCCATCGTCGATCGGCAGTTCGTCGAGGTCATCATCGCTCCGGGCAT
CAGCGACGAGGCTGCCGACATCGTCGCCGAGAAGAAGAATGTCCGCCTGCTCGATGTCAGCGACCATTGGCCCGGGCAGC
GTCGCCCCTCGCACGACTTCAAGCGCGTCACCGGCGGCTTGCTGGTGCAGGACCGTGACCTGGGCATGGTCGAGCGGGAG
GAACTGCGCACCGTCACCGAGCGCGCGCCCAGCGAGCAGGAAATGAACGACCTCAGCTTCGCCTGGAAAGTCGCCAAGTA
CGTCAAGTCCAATGCCATCGTCTACGCCAAGCAAGGGCAGACCATCGGGGTCGGTGCCGGTCAGATGAGCCGCGTCTATT
CCGCCAAGATCGCGGGCATCAAGGCCGCCGACGAGCATCTGGAAGTGCCCGGCTCGGTGATGGCCTCGGATGCCTTCTTC
CCGTTCCGCGACGGCATCGATGCCGCGGCCCAGGCCGGCATCACCGCCGTGATCCAGCCCGGCGGCTCCATGCGCGATCA
GGAAGTGATCGATGCCGCCAACGAAGCCGGCATCGCCATGGTCTTCACCGGCATGCGTCACTTCCGGCACTGA

Upstream 100 bases:

>100_bases
GAACTGATTTGATTCCGTCCCGGGGCGTCGCGTCAGCGACGCCCCGTTGCGTTTCGTCCGGCGCCCATCGCGCGCCCTCA
CCTTGCAAGAGTGGAGCCCA

Downstream 100 bases:

>100_bases
GACCGGACTGCGCGCCAACGTGAAAACGGCCCGCCCCGAATGGCATTCGGGGCGGGCCGTTGGCGTTACGAGTGATGGAT
GTACCTCGCCGCCCCATGCA

Product: IMP cyclohydrolase / phosphoribosylaminoimidazolecarboxamide formyltransferase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MAEHTTPSSARPVRRALISVSDKTGIVEFARELAARGVALLSTGGTFRLLSEHGIPVTEVSQHTGFPEIMDGRVKTLHPK
IHGGILGRRGQDDEVMAAHDIDAIDMVVVNLYPFADTVAREDCTLEEAIENIDIGGPTMVRACAKNHAHTTIVVNAGDYS
RVLDDMSAQGGAVGQALRFDLAVKAFEHTAGYDGAIANYLGTLAEGGEANFPRTYNVQFHKKQAMRYGENPHQQAAFYAE
ADAAEASVTTARQRQGKALSFNNVADTDAAFECVKAFRETACVIVKHANPCGVAVAETPLAAYERAFATDPTSAFGGIIA
FNRALDAATARAIVDRQFVEVIIAPGISDEAADIVAEKKNVRLLDVSDHWPGQRRPSHDFKRVTGGLLVQDRDLGMVERE
ELRTVTERAPSEQEMNDLSFAWKVAKYVKSNAIVYAKQGQTIGVGAGQMSRVYSAKIAGIKAADEHLEVPGSVMASDAFF
PFRDGIDAAAQAGITAVIQPGGSMRDQEVIDAANEAGIAMVFTGMRHFRH

Sequences:

>Translated_530_residues
MAEHTTPSSARPVRRALISVSDKTGIVEFARELAARGVALLSTGGTFRLLSEHGIPVTEVSQHTGFPEIMDGRVKTLHPK
IHGGILGRRGQDDEVMAAHDIDAIDMVVVNLYPFADTVAREDCTLEEAIENIDIGGPTMVRACAKNHAHTTIVVNAGDYS
RVLDDMSAQGGAVGQALRFDLAVKAFEHTAGYDGAIANYLGTLAEGGEANFPRTYNVQFHKKQAMRYGENPHQQAAFYAE
ADAAEASVTTARQRQGKALSFNNVADTDAAFECVKAFRETACVIVKHANPCGVAVAETPLAAYERAFATDPTSAFGGIIA
FNRALDAATARAIVDRQFVEVIIAPGISDEAADIVAEKKNVRLLDVSDHWPGQRRPSHDFKRVTGGLLVQDRDLGMVERE
ELRTVTERAPSEQEMNDLSFAWKVAKYVKSNAIVYAKQGQTIGVGAGQMSRVYSAKIAGIKAADEHLEVPGSVMASDAFF
PFRDGIDAAAQAGITAVIQPGGSMRDQEVIDAANEAGIAMVFTGMRHFRH
>Mature_529_residues
AEHTTPSSARPVRRALISVSDKTGIVEFARELAARGVALLSTGGTFRLLSEHGIPVTEVSQHTGFPEIMDGRVKTLHPKI
HGGILGRRGQDDEVMAAHDIDAIDMVVVNLYPFADTVAREDCTLEEAIENIDIGGPTMVRACAKNHAHTTIVVNAGDYSR
VLDDMSAQGGAVGQALRFDLAVKAFEHTAGYDGAIANYLGTLAEGGEANFPRTYNVQFHKKQAMRYGENPHQQAAFYAEA
DAAEASVTTARQRQGKALSFNNVADTDAAFECVKAFRETACVIVKHANPCGVAVAETPLAAYERAFATDPTSAFGGIIAF
NRALDAATARAIVDRQFVEVIIAPGISDEAADIVAEKKNVRLLDVSDHWPGQRRPSHDFKRVTGGLLVQDRDLGMVEREE
LRTVTERAPSEQEMNDLSFAWKVAKYVKSNAIVYAKQGQTIGVGAGQMSRVYSAKIAGIKAADEHLEVPGSVMASDAFFP
FRDGIDAAAQAGITAVIQPGGSMRDQEVIDAANEAGIAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=485, Percent_Identity=39.3814432989691, Blast_Score=284, Evalue=1e-76,
Organism=Escherichia coli, GI1790439, Length=527, Percent_Identity=71.3472485768501, Blast_Score=748, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=614, Percent_Identity=31.2703583061889, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71985574, Length=353, Percent_Identity=28.0453257790368, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985556, Length=152, Percent_Identity=32.8947368421053, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323056, Length=487, Percent_Identity=36.9609856262834, Blast_Score=284, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6323768, Length=479, Percent_Identity=35.9081419624217, Blast_Score=270, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24649832, Length=506, Percent_Identity=37.7470355731225, Blast_Score=274, Evalue=1e-73,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57009; Mature: 56878

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHTTPSSARPVRRALISVSDKTGIVEFARELAARGVALLSTGGTFRLLSEHGIPVTEV
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCHHHH
SQHTGFPEIMDGRVKTLHPKIHGGILGRRGQDDEVMAAHDIDAIDMVVVNLYPFADTVAR
HHHCCCHHHHCCCCEEECCHHHCCCCCCCCCCCCEEEECCCCHHEEEEEEHCCCHHHHHH
EDCTLEEAIENIDIGGPTMVRACAKNHAHTTIVVNAGDYSRVLDDMSAQGGAVGQALRFD
CCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHH
LAVKAFEHTAGYDGAIANYLGTLAEGGEANFPRTYNVQFHKKQAMRYGENPHQQAAFYAE
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHCCCCHHHHHHEEEC
ADAAEASVTTARQRQGKALSFNNVADTDAAFECVKAFRETACVIVKHANPCGVAVAETPL
CCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCCCCEEEECCCH
AAYERAFATDPTSAFGGIIAFNRALDAATARAIVDRQFVEVIIAPGISDEAADIVAEKKN
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCC
VRLLDVSDHWPGQRRPSHDFKRVTGGLLVQDRDLGMVEREELRTVTERAPSEQEMNDLSF
EEEEEECCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
AWKVAKYVKSNAIVYAKQGQTIGVGAGQMSRVYSAKIAGIKAADEHLEVPGSVMASDAFF
HHHHHHHHHCCEEEEEECCCEEEECCCHHHHHHHHHHCCCEECHHHHCCCCCHHCCCCCC
PFRDGIDAAAQAGITAVIQPGGSMRDQEVIDAANEAGIAMVFTGMRHFRH
CCCCCCHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCEEHEEHHHHHHCC
>Mature Secondary Structure 
AEHTTPSSARPVRRALISVSDKTGIVEFARELAARGVALLSTGGTFRLLSEHGIPVTEV
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCHHHH
SQHTGFPEIMDGRVKTLHPKIHGGILGRRGQDDEVMAAHDIDAIDMVVVNLYPFADTVAR
HHHCCCHHHHCCCCEEECCHHHCCCCCCCCCCCCEEEECCCCHHEEEEEEHCCCHHHHHH
EDCTLEEAIENIDIGGPTMVRACAKNHAHTTIVVNAGDYSRVLDDMSAQGGAVGQALRFD
CCCCHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHH
LAVKAFEHTAGYDGAIANYLGTLAEGGEANFPRTYNVQFHKKQAMRYGENPHQQAAFYAE
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHCCCCHHHHHHEEEC
ADAAEASVTTARQRQGKALSFNNVADTDAAFECVKAFRETACVIVKHANPCGVAVAETPL
CCCCHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCCCCEEEECCCH
AAYERAFATDPTSAFGGIIAFNRALDAATARAIVDRQFVEVIIAPGISDEAADIVAEKKN
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCC
VRLLDVSDHWPGQRRPSHDFKRVTGGLLVQDRDLGMVEREELRTVTERAPSEQEMNDLSF
EEEEEECCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
AWKVAKYVKSNAIVYAKQGQTIGVGAGQMSRVYSAKIAGIKAADEHLEVPGSVMASDAFF
HHHHHHHHHCCEEEEEECCCEEEECCCHHHHHHHHHHCCCEECHHHHCCCCCHHCCCCCC
PFRDGIDAAAQAGITAVIQPGGSMRDQEVIDAANEAGIAMVFTGMRHFRH
CCCCCCHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCEEHEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA