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The map label for this gene is ptsN [H]

Identifier: 92114350

GI number: 92114350

Start: 2499369

End: 2499863

Strand: Direct

Name: ptsN [H]

Synonym: Csal_2228

Alternate gene names: 92114350

Gene position: 2499369-2499863 (Clockwise)

Preceding gene: 92114349

Following gene: 92114351

Centisome position: 67.61

GC content: 62.63

Gene sequence:

>495_bases
GTGCCCGTTGAATCCGCCATGACACTGGAAAGCATCCTGCCCCCCGAGCGCGCCCTCTTCGGCGTTGCCGGGGGGAGCAA
GAAGCGTGTGCTGGAATTCTTCAGCACCTTCATCGCCCAGAATACGCCTAGCCTCGACAGCCAGGAGGTCTTCGGCCGTC
TCATCGGTCGTGAACGCCTGGGAAGCACCGGCATCGGCAATGGCGTTGCCATTCCGCATGCGCGCAACCCGCATTGCAAG
GCGCCCATCGCCGGTTTTCTGCGTCTTCAGGACCCCATCGACTTCGATGCGATAGACGGCGAGCCCGTCGACCTGATATT
TGTGCTGCTGGTCCCGGAAGAAGCCGATGACGCCCACCTGTCGCTGCTCGCCCAGGTCGCTGCCATCATGAACGACAGCG
CCATCCGTAGCAGGCTGCGACAAGCGCAGACCCAACGGCAACTCTACGATACCCTGATCGAGGCCATACGCCAGCAAGAC
ACCCGCGCCAGCTGA

Upstream 100 bases:

>100_bases
AGCGACGACATGTACGCTGCCATCGACGCCCTCACCGACAAGCTCGACCGCCAGCTCGTCAAACACAAAGAAAAGATGCA
GGCACGCGCCCAGGGCGCCG

Downstream 100 bases:

>100_bases
CGCTGGCGCAAACCAAGGGGCGCGCCCATGCAACTCGTGATCATCAGCGGCCGTTCCGGCTCCGGTAAATCCATTGCCTT
GCAGGCACTCGAGGATCTCG

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MPVESAMTLESILPPERALFGVAGGSKKRVLEFFSTFIAQNTPSLDSQEVFGRLIGRERLGSTGIGNGVAIPHARNPHCK
APIAGFLRLQDPIDFDAIDGEPVDLIFVLLVPEEADDAHLSLLAQVAAIMNDSAIRSRLRQAQTQRQLYDTLIEAIRQQD
TRAS

Sequences:

>Translated_164_residues
MPVESAMTLESILPPERALFGVAGGSKKRVLEFFSTFIAQNTPSLDSQEVFGRLIGRERLGSTGIGNGVAIPHARNPHCK
APIAGFLRLQDPIDFDAIDGEPVDLIFVLLVPEEADDAHLSLLAQVAAIMNDSAIRSRLRQAQTQRQLYDTLIEAIRQQD
TRAS
>Mature_163_residues
PVESAMTLESILPPERALFGVAGGSKKRVLEFFSTFIAQNTPSLDSQEVFGRLIGRERLGSTGIGNGVAIPHARNPHCKA
PIAGFLRLQDPIDFDAIDGEPVDLIFVLLVPEEADDAHLSLLAQVAAIMNDSAIRSRLRQAQTQRQLYDTLIEAIRQQDT
RAS

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=147, Percent_Identity=44.2176870748299, Blast_Score=111, Evalue=2e-26,
Organism=Escherichia coli, GI2367327, Length=129, Percent_Identity=29.4573643410853, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI1786951, Length=98, Percent_Identity=34.6938775510204, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17921; Mature: 17789

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVESAMTLESILPPERALFGVAGGSKKRVLEFFSTFIAQNTPSLDSQEVFGRLIGRERL
CCCCHHHHHHHHCCHHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
GSTGIGNGVAIPHARNPHCKAPIAGFLRLQDPIDFDAIDGEPVDLIFVLLVPEEADDAHL
CCCCCCCCEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHH
SLLAQVAAIMNDSAIRSRLRQAQTQRQLYDTLIEAIRQQDTRAS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PVESAMTLESILPPERALFGVAGGSKKRVLEFFSTFIAQNTPSLDSQEVFGRLIGRERL
CCCHHHHHHHHCCHHHHEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
GSTGIGNGVAIPHARNPHCKAPIAGFLRLQDPIDFDAIDGEPVDLIFVLLVPEEADDAHL
CCCCCCCCEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHH
SLLAQVAAIMNDSAIRSRLRQAQTQRQLYDTLIEAIRQQDTRAS
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]