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The map label for this gene is rpoN [H]

Identifier: 92114348

GI number: 92114348

Start: 2497501

End: 2498934

Strand: Direct

Name: rpoN [H]

Synonym: Csal_2226

Alternate gene names: 92114348

Gene position: 2497501-2498934 (Clockwise)

Preceding gene: 92114347

Following gene: 92114349

Centisome position: 67.56

GC content: 63.39

Gene sequence:

>1434_bases
ATGTCCATGAAACCTACGCTTCAGCTTCGCGTCGGCACCCAGCTGACGATGACGCCTCAGTTGCAGCAAGCGATCCAGCT
CTTGCAGCTCTCGACGCTGGATTTGCGTCAGGAGATCCAGCAGGCGCTGGACGCCAACCCCATGCTGGAACTCGAGGAAC
ACTTCGACGAGCGCGAAGTCAGCGAGACGCAGGACGACGACTGGAGCAGCGAAATTCCGGAAGACCTGCCGCTGGACAGC
GACTGGAGCGATACCTACCAGGACGCCGGGCTCGGCTCATTGAACGGCAGCGGGGGCAGCGAGGAAGGCCCCGACATCGA
GCGCAACACGGCGCACACCAGCCTTCACGATCATCTGGGCTGGCAACTGGCCATGACCGACATGACGCCACGCGAACATG
CGATCGCCGAGAGTCTGATCGATGCCGTGGGCGCCGATGGCTACCTTACCGTGTCGCTCGAGGAGCTTCTCGATGGGCTG
CGCGGTCAGGGGTTGGCCGGCCTCAAGATCGGTGACGTCGAGCAGGTCATGATGCGCGTGCAGCAATTCGACCCCACGGG
CGTCGCCGCTCGCGATCTGCGCGAATGTCTCCTGCTGCAGCTGGGCGCCCTGCCCGAGGACATGCCGCTGCTTCCCCAGG
CCAAGCGCCTGGTGCGTCAATTCCTCGACGCGCTCGCCGGCAACGACCGCAAGCTGCTCAAACGGCGCCTGCGTCTCGAA
GAGGCGGAACTCGACCATATCATCGCCCTCATCCGCACGCTCGACCCGCGCCCCGGTCTGGCCTTCGAGGACAGCGACAA
CGATTACGTCATCCCCGACCTCATCGCCCGCCGCGTTCGTCAGGAATGGCGCATCGAGCTCAATCCCGACGCCTTGCCGC
GCGTCCGCATCCAGCCGGATTACGCCGCTCTGATCAAGCGTGCCGACAAGAGTGACGACAACACCTTCCTCAAGCAGCAC
CTGCAGGAAGCCAAGTGGCTGCTCAAGAGCCTGTCTAGCCGTAACGACACCCTGTTGCGCGTGGGCCGCGAAATCATGGC
CCGTCAGCTCGACTTCCTCGAGCATGGCGAAGAAGCGATGAAACCGTTGATCCTCGCCGATATCGCCGGCGCGGTGGACA
TGCACGAATCGACCATTTCACGCGTCACGACGCAGAAATTCATTCATACCCCACGTGGCGTGTTCGAGCTGAAATACTTC
TTCTCCAGCCATGTGGGCGGTGGCCACGGCGAAGGCGACGCCCATTCCAGCACCGCGATACGCGCCCGGCTGAAGAAGCT
GATCGGCGAAGAGCCGCCACGCAAGCCGTTGTCCGACAGCAAGCTCGTCGACCTGCTGGCACAGGACGGCATTCAGGTCG
CACGCCGCACGGTGGCCAAGTATCGTGAAGCGATGGGCATCCCCTCCTCCAGCGAACGCAAGCGCCTGGCCTGA

Upstream 100 bases:

>100_bases
GGCTCATATAAATGGCATGGTTGTTGCTTCCATTCTTGCAACTCACGTCGCTGCGGGATAACGTACGGGCACCGTTTCAG
GAAGCGACGACCGACTCTCC

Downstream 100 bases:

>100_bases
ACGCCCAACGTTTGCACCCGTCGACGCCGCTGCGTAGACTCGCCGCGGTCGTCGACACCCAGGCATCGCCCGGGGCTATG
CATTGCCCTAAAAAGGGTTA

Product: sigma-54 RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSMKPTLQLRVGTQLTMTPQLQQAIQLLQLSTLDLRQEIQQALDANPMLELEEHFDEREVSETQDDDWSSEIPEDLPLDS
DWSDTYQDAGLGSLNGSGGSEEGPDIERNTAHTSLHDHLGWQLAMTDMTPREHAIAESLIDAVGADGYLTVSLEELLDGL
RGQGLAGLKIGDVEQVMMRVQQFDPTGVAARDLRECLLLQLGALPEDMPLLPQAKRLVRQFLDALAGNDRKLLKRRLRLE
EAELDHIIALIRTLDPRPGLAFEDSDNDYVIPDLIARRVRQEWRIELNPDALPRVRIQPDYAALIKRADKSDDNTFLKQH
LQEAKWLLKSLSSRNDTLLRVGREIMARQLDFLEHGEEAMKPLILADIAGAVDMHESTISRVTTQKFIHTPRGVFELKYF
FSSHVGGGHGEGDAHSSTAIRARLKKLIGEEPPRKPLSDSKLVDLLAQDGIQVARRTVAKYREAMGIPSSSERKRLA

Sequences:

>Translated_477_residues
MSMKPTLQLRVGTQLTMTPQLQQAIQLLQLSTLDLRQEIQQALDANPMLELEEHFDEREVSETQDDDWSSEIPEDLPLDS
DWSDTYQDAGLGSLNGSGGSEEGPDIERNTAHTSLHDHLGWQLAMTDMTPREHAIAESLIDAVGADGYLTVSLEELLDGL
RGQGLAGLKIGDVEQVMMRVQQFDPTGVAARDLRECLLLQLGALPEDMPLLPQAKRLVRQFLDALAGNDRKLLKRRLRLE
EAELDHIIALIRTLDPRPGLAFEDSDNDYVIPDLIARRVRQEWRIELNPDALPRVRIQPDYAALIKRADKSDDNTFLKQH
LQEAKWLLKSLSSRNDTLLRVGREIMARQLDFLEHGEEAMKPLILADIAGAVDMHESTISRVTTQKFIHTPRGVFELKYF
FSSHVGGGHGEGDAHSSTAIRARLKKLIGEEPPRKPLSDSKLVDLLAQDGIQVARRTVAKYREAMGIPSSSERKRLA
>Mature_476_residues
SMKPTLQLRVGTQLTMTPQLQQAIQLLQLSTLDLRQEIQQALDANPMLELEEHFDEREVSETQDDDWSSEIPEDLPLDSD
WSDTYQDAGLGSLNGSGGSEEGPDIERNTAHTSLHDHLGWQLAMTDMTPREHAIAESLIDAVGADGYLTVSLEELLDGLR
GQGLAGLKIGDVEQVMMRVQQFDPTGVAARDLRECLLLQLGALPEDMPLLPQAKRLVRQFLDALAGNDRKLLKRRLRLEE
AELDHIIALIRTLDPRPGLAFEDSDNDYVIPDLIARRVRQEWRIELNPDALPRVRIQPDYAALIKRADKSDDNTFLKQHL
QEAKWLLKSLSSRNDTLLRVGREIMARQLDFLEHGEEAMKPLILADIAGAVDMHESTISRVTTQKFIHTPRGVFELKYFF
SSHVGGGHGEGDAHSSTAIRARLKKLIGEEPPRKPLSDSKLVDLLAQDGIQVARRTVAKYREAMGIPSSSERKRLA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=483, Percent_Identity=51.1387163561077, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53505; Mature: 53373

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMKPTLQLRVGTQLTMTPQLQQAIQLLQLSTLDLRQEIQQALDANPMLELEEHFDEREV
CCCCCCEEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHH
SETQDDDWSSEIPEDLPLDSDWSDTYQDAGLGSLNGSGGSEEGPDIERNTAHTSLHDHLG
HCCCCCCCHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
WQLAMTDMTPREHAIAESLIDAVGADGYLTVSLEELLDGLRGQGLAGLKIGDVEQVMMRV
CEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCEECCHHHHHHHH
QQFDPTGVAARDLRECLLLQLGALPEDMPLLPQAKRLVRQFLDALAGNDRKLLKRRLRLE
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EAELDHIIALIRTLDPRPGLAFEDSDNDYVIPDLIARRVRQEWRIELNPDALPRVRIQPD
HHHHHHHHHHHHHCCCCCCCCEECCCCCEECHHHHHHHHHHHCCEECCCCCCCCEEECCC
YAALIKRADKSDDNTFLKQHLQEAKWLLKSLSSRNDTLLRVGREIMARQLDFLEHGEEAM
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
KPLILADIAGAVDMHESTISRVTTQKFIHTPRGVFELKYFFSSHVGGGHGEGDAHSSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RARLKKLIGEEPPRKPLSDSKLVDLLAQDGIQVARRTVAKYREAMGIPSSSERKRLA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SMKPTLQLRVGTQLTMTPQLQQAIQLLQLSTLDLRQEIQQALDANPMLELEEHFDEREV
CCCCCEEEEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHH
SETQDDDWSSEIPEDLPLDSDWSDTYQDAGLGSLNGSGGSEEGPDIERNTAHTSLHDHLG
HCCCCCCCHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
WQLAMTDMTPREHAIAESLIDAVGADGYLTVSLEELLDGLRGQGLAGLKIGDVEQVMMRV
CEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCEECCHHHHHHHH
QQFDPTGVAARDLRECLLLQLGALPEDMPLLPQAKRLVRQFLDALAGNDRKLLKRRLRLE
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EAELDHIIALIRTLDPRPGLAFEDSDNDYVIPDLIARRVRQEWRIELNPDALPRVRIQPD
HHHHHHHHHHHHHCCCCCCCCEECCCCCEECHHHHHHHHHHHCCEECCCCCCCCEEECCC
YAALIKRADKSDDNTFLKQHLQEAKWLLKSLSSRNDTLLRVGREIMARQLDFLEHGEEAM
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
KPLILADIAGAVDMHESTISRVTTQKFIHTPRGVFELKYFFSSHVGGGHGEGDAHSSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RARLKKLIGEEPPRKPLSDSKLVDLLAQDGIQVARRTVAKYREAMGIPSSSERKRLA
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113171; 10984043 [H]