Click here to switch to the map view.

The map label for this gene is pilB [H]

Identifier: 92114296

GI number: 92114296

Start: 2447058

End: 2448797

Strand: Direct

Name: pilB [H]

Synonym: Csal_2174

Alternate gene names: 92114296

Gene position: 2447058-2448797 (Clockwise)

Preceding gene: 92114294

Following gene: 92114297

Centisome position: 66.2

GC content: 65.11

Gene sequence:

>1740_bases
ATGCCATTGTCTTCATCATCCACCTCCGATACGGAGGCATCACCGCTGAAAGGGCTCGCCGCGCGCCTGGTGCAGGAAGG
TCTGCTCGAGGAACAGCAGGCGCGCAGCGCCCTGGAGGCCGTACGCGAAAGCCACCAGTCGATGCTCGAGTATGTCATCG
AGCGCCAGTGGGTCCCTGCCCGCGCGGCGACCCAGGTTGCGGCCTGGGAGTACGGTCTGCCGTTCGTCGATCTCGAGGCC
GTCGACCTCGGCCGGCTCCCCACCGGTGAGGGCCTGCCCGATGCGATGATACGTCGTCTGGGCATACTGCCCCTGGCGCG
CAGCGAGCGGCATATCAGCGTGGCGGTTCCCTACCCCGCGACCCTGGCGAAACTGGACGAACTGCAGTTCAGTACCGGCC
TTTCCGCCGAGGGGGTCCTGGTCGCCGCCGATCAGTTGCAACCCGCCATCGACAGCCACCTTGCCCAGCGCGAGACGCAC
GGCATGCTCGAGGCCCTTGGCGATGCCAGCGAAGCGCTCGAGACGCTGGATCTCGACGATGTCGAGCCTCTCGAGGATGG
GCTCGGTACCGAGGACGACGATGCGCCGGTGGTGCGCTTCGTGCACAAGATGATGATCGATGCGGCCCGTCGCGGCGCCT
CGGACATTCACTTCGAGCCGTACGAGTCGACCTTTCGCATTCGTTTTCGCATCGATGGCATCCTGGTCGAGGTCGCACGC
CCACCATTCAACCTGCGTGCGCGTCTGTCGGCACGCCTCAAGGTCATGTCGCGCCTGGACATCTCCGAACGCCGCCTGCC
TCAGGACGGCGCGATCAAGCTGCGGCTGTCGGCATCGCGAACCATCGATTTCCGCGTCAGCACCTTGCCCACGGTCCATG
GCGAGAAGATCGTGCTGCGTCTGCTGGATGCCGGCGCCACGCGCCTGGGGATCGATGCACTGGGGTTCAGCGAGGCGCAG
CGCGCGATGTTCGAGCACGCCCTGGCACAGCCCCAGGGCATGATTCTCGCCACCGGCCCCACCGGCAGCGGCAAGACCGT
GACCTTGTACACTGGGCTCAATATCCTCAACACCGACGCGCGCAACATTTCCACCGCCGAGGATCCCGTCGAACTCAAGC
TCGACGGCATCAACCAGGTCAGCGTTCAGCCGAAGATCGGGCTGGATTTCGCCAATGCCTTGCGCGCCTTCCTGCGCCAG
GACCCCGACGTGGTCATGGTCGGCGAGGTGCGCGACCTGGAGACCGCCGAAATCGCCGTCAAGGCCGCGCAAACCGGACA
CCTGGTGCTCTCGACGCTGCATACGAATTCGGCGGCGGAAACGCTGACGCGCCTGCGCAACATGGGGGTGGCGGCCTACA
ACATCGCCAGTTCGGTGAGCCTGATCGTCGCCCAGCGGCTCGTGCGACAACTCTGCCCGCACTGCAAGACGCCCGTCGAG
CTGCCCCGGGAGCTCTTCATCGAGGCCGGCTTCACGTCCACCGACATCGAATCGACGACCTGCTATCGTGCCGCGGGCTG
CGCCCAGTGTACCCACGGGTACAAGGGACGCGTGGGAATCTACGAGGTACTGCCCGTCAGCGAGGCCATGAGCAAGTTGA
TCATGGCCGACGGCAATTCTCTGGAACTGGGGGAACTGGCCCGTCAAGAAGGCCACCCCGATTTAAGACGCAGCGGACTG
AGCAAGGTGCTCGCCGGCATCACCAGCCTCGAGGAAATCAACCGCGTGGTGCTGGAATGA

Upstream 100 bases:

>100_bases
TACCAAGGGTCACCAAGCGCCCATGCATGGGGTAGGATGCGACGAGTAGCGGGCGGTGACGCCGCCCCGTTTGGTCACTG
CCCTGGGTGTCGCAACGCCT

Downstream 100 bases:

>100_bases
AAGCTCAGGGCAAGTGACAAGAGGGGAACGACATGGCGGTAGCGACACGTAGCAAGGCGCCACGGCGCGTCGTCCTGCAT
CGCTGGCGATGGAAGGGCAA

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MPLSSSSTSDTEASPLKGLAARLVQEGLLEEQQARSALEAVRESHQSMLEYVIERQWVPARAATQVAAWEYGLPFVDLEA
VDLGRLPTGEGLPDAMIRRLGILPLARSERHISVAVPYPATLAKLDELQFSTGLSAEGVLVAADQLQPAIDSHLAQRETH
GMLEALGDASEALETLDLDDVEPLEDGLGTEDDDAPVVRFVHKMMIDAARRGASDIHFEPYESTFRIRFRIDGILVEVAR
PPFNLRARLSARLKVMSRLDISERRLPQDGAIKLRLSASRTIDFRVSTLPTVHGEKIVLRLLDAGATRLGIDALGFSEAQ
RAMFEHALAQPQGMILATGPTGSGKTVTLYTGLNILNTDARNISTAEDPVELKLDGINQVSVQPKIGLDFANALRAFLRQ
DPDVVMVGEVRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLRNMGVAAYNIASSVSLIVAQRLVRQLCPHCKTPVE
LPRELFIEAGFTSTDIESTTCYRAAGCAQCTHGYKGRVGIYEVLPVSEAMSKLIMADGNSLELGELARQEGHPDLRRSGL
SKVLAGITSLEEINRVVLE

Sequences:

>Translated_579_residues
MPLSSSSTSDTEASPLKGLAARLVQEGLLEEQQARSALEAVRESHQSMLEYVIERQWVPARAATQVAAWEYGLPFVDLEA
VDLGRLPTGEGLPDAMIRRLGILPLARSERHISVAVPYPATLAKLDELQFSTGLSAEGVLVAADQLQPAIDSHLAQRETH
GMLEALGDASEALETLDLDDVEPLEDGLGTEDDDAPVVRFVHKMMIDAARRGASDIHFEPYESTFRIRFRIDGILVEVAR
PPFNLRARLSARLKVMSRLDISERRLPQDGAIKLRLSASRTIDFRVSTLPTVHGEKIVLRLLDAGATRLGIDALGFSEAQ
RAMFEHALAQPQGMILATGPTGSGKTVTLYTGLNILNTDARNISTAEDPVELKLDGINQVSVQPKIGLDFANALRAFLRQ
DPDVVMVGEVRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLRNMGVAAYNIASSVSLIVAQRLVRQLCPHCKTPVE
LPRELFIEAGFTSTDIESTTCYRAAGCAQCTHGYKGRVGIYEVLPVSEAMSKLIMADGNSLELGELARQEGHPDLRRSGL
SKVLAGITSLEEINRVVLE
>Mature_578_residues
PLSSSSTSDTEASPLKGLAARLVQEGLLEEQQARSALEAVRESHQSMLEYVIERQWVPARAATQVAAWEYGLPFVDLEAV
DLGRLPTGEGLPDAMIRRLGILPLARSERHISVAVPYPATLAKLDELQFSTGLSAEGVLVAADQLQPAIDSHLAQRETHG
MLEALGDASEALETLDLDDVEPLEDGLGTEDDDAPVVRFVHKMMIDAARRGASDIHFEPYESTFRIRFRIDGILVEVARP
PFNLRARLSARLKVMSRLDISERRLPQDGAIKLRLSASRTIDFRVSTLPTVHGEKIVLRLLDAGATRLGIDALGFSEAQR
AMFEHALAQPQGMILATGPTGSGKTVTLYTGLNILNTDARNISTAEDPVELKLDGINQVSVQPKIGLDFANALRAFLRQD
PDVVMVGEVRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLRNMGVAAYNIASSVSLIVAQRLVRQLCPHCKTPVEL
PRELFIEAGFTSTDIESTTCYRAAGCAQCTHGYKGRVGIYEVLPVSEAMSKLIMADGNSLELGELARQEGHPDLRRSGLS
KVLAGITSLEEINRVVLE

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=412, Percent_Identity=48.3009708737864, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1786296, Length=485, Percent_Identity=42.2680412371134, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI87082188, Length=246, Percent_Identity=35.7723577235772, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62936; Mature: 62804

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSSSSTSDTEASPLKGLAARLVQEGLLEEQQARSALEAVRESHQSMLEYVIERQWVPA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
RAATQVAAWEYGLPFVDLEAVDLGRLPTGEGLPDAMIRRLGILPLARSERHISVAVPYPA
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCH
TLAKLDELQFSTGLSAEGVLVAADQLQPAIDSHLAQRETHGMLEALGDASEALETLDLDD
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
VEPLEDGLGTEDDDAPVVRFVHKMMIDAARRGASDIHFEPYESTFRIRFRIDGILVEVAR
CCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEEECC
PPFNLRARLSARLKVMSRLDISERRLPQDGAIKLRLSASRTIDFRVSTLPTVHGEKIVLR
CCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHH
LLDAGATRLGIDALGFSEAQRAMFEHALAQPQGMILATGPTGSGKTVTLYTGLNILNTDA
HHHCCCCEECCHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEECCCC
RNISTAEDPVELKLDGINQVSVQPKIGLDFANALRAFLRQDPDVVMVGEVRDLETAEIAV
CCCCCCCCCCEEEECCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHEE
KAAQTGHLVLSTLHTNSAAETLTRLRNMGVAAYNIASSVSLIVAQRLVRQLCPHCKTPVE
ECCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LPRELFIEAGFTSTDIESTTCYRAAGCAQCTHGYKGRVGIYEVLPVSEAMSKLIMADGNS
CHHHHHEECCCCCCCCCCCHHHHHCCHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCCC
LELGELARQEGHPDLRRSGLSKVLAGITSLEEINRVVLE
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLSSSSTSDTEASPLKGLAARLVQEGLLEEQQARSALEAVRESHQSMLEYVIERQWVPA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
RAATQVAAWEYGLPFVDLEAVDLGRLPTGEGLPDAMIRRLGILPLARSERHISVAVPYPA
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCH
TLAKLDELQFSTGLSAEGVLVAADQLQPAIDSHLAQRETHGMLEALGDASEALETLDLDD
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCC
VEPLEDGLGTEDDDAPVVRFVHKMMIDAARRGASDIHFEPYESTFRIRFRIDGILVEVAR
CCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEEECC
PPFNLRARLSARLKVMSRLDISERRLPQDGAIKLRLSASRTIDFRVSTLPTVHGEKIVLR
CCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHH
LLDAGATRLGIDALGFSEAQRAMFEHALAQPQGMILATGPTGSGKTVTLYTGLNILNTDA
HHHCCCCEECCHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEECCCC
RNISTAEDPVELKLDGINQVSVQPKIGLDFANALRAFLRQDPDVVMVGEVRDLETAEIAV
CCCCCCCCCCEEEECCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHEE
KAAQTGHLVLSTLHTNSAAETLTRLRNMGVAAYNIASSVSLIVAQRLVRQLCPHCKTPVE
ECCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LPRELFIEAGFTSTDIESTTCYRAAGCAQCTHGYKGRVGIYEVLPVSEAMSKLIMADGNS
CHHHHHEECCCCCCCCCCCHHHHHCCHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCCC
LELGELARQEGHPDLRRSGLSKVLAGITSLEEINRVVLE
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]