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The map label for this gene is trmJ [H]

Identifier: 92114283

GI number: 92114283

Start: 2429930

End: 2430670

Strand: Direct

Name: trmJ [H]

Synonym: Csal_2161

Alternate gene names: 92114283

Gene position: 2429930-2430670 (Clockwise)

Preceding gene: 92114282

Following gene: 92114291

Centisome position: 65.73

GC content: 63.83

Gene sequence:

>741_bases
ATGCTCTCCAATATTCGCATCGTACTCGTCCAGACCTTTCACCCCGGCAATATCGGCCAGGCGGCACGCGCCATGAAAAC
CATGGGCCTCAGCGACCTGGTGCTGGTCAATCCGCGCAGCTTTCCGGACGCGGAAGCGACTCGGCTGGCCGCAGGCGCCA
CCGATGTCATCGACAACGCACGCGTCGTCGAACACATCGCGCAAGCCGTGGGTGACTGCGTGCAGGTGGTAGGTGCCAGC
GCGCGGCTGCGCAGCCTTCCCCTGCCGCATTTCGACGACCCCGAGCCCATGGCGTCCGAGCTGATCGAGCATGCACAAGA
CGCCCCGGTAGCCCTGGTATTCGGTCGCGAACGCTTCGGGCTGACGAATGATGAGATTCGCCATTGCACGCACCAGGTCA
GCATCCCTGCCAACCCTGAATATGGCGTGCTCAACCTCGCCCAGGCGGTGCAGATCCTGGCCTACGAGGTGCGCCGAGCC
TGGCGTCATCACCAGGCCAGCCCCGCGACCTCGCAAACACCGCCCTCGCTACCGACCCGCGACCAGATGGCCCATTTCGA
GGCGCATTTGCACGAGGCCCTGGTGACATCGGGTTTCCTGACGCAACCTCATGCGCGTACCGAGGATCACCTGCGCGCAC
TCTTCGCGCGTGCACAGCCATCCCGCAAGGAGCTGTCCATTCTACGAGGCGTGTTATCGACGCTGGAGAAAGCGGGCAGT
AATCCGCGTTCCTCCTCGTAG

Upstream 100 bases:

>100_bases
GTTGCCGGCCGGTCACCGGTGCCGTAGCCCGTAGCCCGTAGCCCGTAGCCCGTAGCCCAAGTACACTATCGCCCTGCCCT
AGACTGCCTCGCGACTTCCT

Downstream 100 bases:

>100_bases
GCCCCGGCGTTGCCCCCGGCCGGACACACTCCGGCCTGGCATTGCCCCGACACATGTCTCTCGCCCCGGCCGCAGCCGGA
TGGTTCCCCTGCGCCCTCTC

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MLSNIRIVLVQTFHPGNIGQAARAMKTMGLSDLVLVNPRSFPDAEATRLAAGATDVIDNARVVEHIAQAVGDCVQVVGAS
ARLRSLPLPHFDDPEPMASELIEHAQDAPVALVFGRERFGLTNDEIRHCTHQVSIPANPEYGVLNLAQAVQILAYEVRRA
WRHHQASPATSQTPPSLPTRDQMAHFEAHLHEALVTSGFLTQPHARTEDHLRALFARAQPSRKELSILRGVLSTLEKAGS
NPRSSS

Sequences:

>Translated_246_residues
MLSNIRIVLVQTFHPGNIGQAARAMKTMGLSDLVLVNPRSFPDAEATRLAAGATDVIDNARVVEHIAQAVGDCVQVVGAS
ARLRSLPLPHFDDPEPMASELIEHAQDAPVALVFGRERFGLTNDEIRHCTHQVSIPANPEYGVLNLAQAVQILAYEVRRA
WRHHQASPATSQTPPSLPTRDQMAHFEAHLHEALVTSGFLTQPHARTEDHLRALFARAQPSRKELSILRGVLSTLEKAGS
NPRSSS
>Mature_246_residues
MLSNIRIVLVQTFHPGNIGQAARAMKTMGLSDLVLVNPRSFPDAEATRLAAGATDVIDNARVVEHIAQAVGDCVQVVGAS
ARLRSLPLPHFDDPEPMASELIEHAQDAPVALVFGRERFGLTNDEIRHCTHQVSIPANPEYGVLNLAQAVQILAYEVRRA
WRHHQASPATSQTPPSLPTRDQMAHFEAHLHEALVTSGFLTQPHARTEDHLRALFARAQPSRKELSILRGVLSTLEKAGS
NPRSSS

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=241, Percent_Identity=50.207468879668, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1790865, Length=154, Percent_Identity=37.012987012987, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26850; Mature: 26850

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNIRIVLVQTFHPGNIGQAARAMKTMGLSDLVLVNPRSFPDAEATRLAAGATDVIDNA
CCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHCCCHHHHHHH
RVVEHIAQAVGDCVQVVGASARLRSLPLPHFDDPEPMASELIEHAQDAPVALVFGRERFG
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHCC
LTNDEIRHCTHQVSIPANPEYGVLNLAQAVQILAYEVRRAWRHHQASPATSQTPPSLPTR
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
DQMAHFEAHLHEALVTSGFLTQPHARTEDHLRALFARAQPSRKELSILRGVLSTLEKAGS
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
NPRSSS
CCCCCC
>Mature Secondary Structure
MLSNIRIVLVQTFHPGNIGQAARAMKTMGLSDLVLVNPRSFPDAEATRLAAGATDVIDNA
CCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHCCCHHHHHHH
RVVEHIAQAVGDCVQVVGASARLRSLPLPHFDDPEPMASELIEHAQDAPVALVFGRERFG
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHCC
LTNDEIRHCTHQVSIPANPEYGVLNLAQAVQILAYEVRRAWRHHQASPATSQTPPSLPTR
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
DQMAHFEAHLHEALVTSGFLTQPHARTEDHLRALFARAQPSRKELSILRGVLSTLEKAGS
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
NPRSSS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA