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The map label for this gene is topB [H]

Identifier: 92114268

GI number: 92114268

Start: 2417369

End: 2419333

Strand: Direct

Name: topB [H]

Synonym: Csal_2146

Alternate gene names: 92114268

Gene position: 2417369-2419333 (Clockwise)

Preceding gene: 92114267

Following gene: 92114269

Centisome position: 65.39

GC content: 68.19

Gene sequence:

>1965_bases
ATGCGCTTGATCATCGCCGAAAAACCCAGCCTGGCACGAGCCATCGCCGATGCCCTGCCAACGGCGCCGCAGCGCCATGA
GGGCTACCTGGTGTGCGGGGAGACCTGCATCAGCTGGTGCGTGGGACATCTCCTCGAGCAGGCACCGCCCGACGCCTACG
ACGCCCGCTATCAGCAATGGCGTCTGGATCATCTGCCCATCGTGCCCCGGCAATGGAAGCTGATCCCGCGTTCCAAGGCG
CGCGCGCAGCTGGCGGTGATTCGCAAGCTGCTCAAGCGTGCCGACACCGTCGTGCACGCCGGCGACCCCGACCGCGAGGG
GCAACTGCTGGTGCAACAGGTGATCGCGCATCTGGGCTGGCGCGGCTCGATCTCGCGCCTGCTGGTCAGCGACCTCAATC
GCCCCGCCGTGCGCCAGGCCCTGCAGTGCATCGAGGACAATGCGCGTTATCAATCGCTGTTCGAGGCCGCCGAGGCCCGT
TCGAGGGCCGACTGGGTATATGGCATCAATCTGTCTCGCGCCTGGACCCTCACCGGTCGCCAGGCCGGCTATCAGGGCGT
CCTGTCGGTCGGCCGCGTGCAAACCCCGGTGCTGGGGCTCATCGTGCGCCGCGACCTGGACATCGAAGCCTTCACGCCAC
GCCCTTTCTACCCCCTGTGGGCGGACCTGAAGGTCGAGCACGGAACCCTGCGTGCCTGGTGGCAACCGCAAGCCCCCCAC
CCACTCGACGAGCAGGGCCGACTGCTCGAGCGCGCCCCGGCCGAGGCCCTGGCGGCCCATCTGCCCGGCGCCGAGGGCCA
CCTGAGCGAGTTGCAGAGCAAGCGACAGACGCAATCGGCACCGTTGCCGTATTCGCTTTCTGCCCTGCAGGTCGATGCCG
CGCGCCGCTACAAGTTGCCGGCCAAGCGCGTGCTGGATATCTGCCAGACACTCTATGAACGCCACCAGTTGATCACCTAT
CCGCGTTCGGATTGCCGCTACCTGCCCGAGGGGCATTTCGCCGCGGCGAAGAAGACGCTGGACACGGCCTGCGCCCAGGA
CGCCACCCTGCGCGGGTGGTATCAAGGGGCGGATTTTTCGCGCCGTTCGCGCGCCTGGAACGACAAGCAGGTCGGCGCCC
ACCACGCCCTGGCACCCACCGGGCGGACGTTCGACGCCTCCCGCCTGACCGGCGACGAGGCCAATGTCTTTCGTCTGATT
GCGCGCAACGTGCTGGCGCAGTTCTATTCACCGCTGGTGACCCGCGACGTCAAGGCAGCGTTCTCGATTCACGGCGAGCA
CTTTCGTGCCCAGGGGCGCGAGATTCTCGAGCCTGGCTGGAAGCCGCTCTTCACGACGCGCGACGAGGCCCCGCCCTTGC
CCGCGCTGCACGAAGGCGAGGCGGCACGGGTCGTCGACTGCGCGGTGGAGGATCGTCAGACACGGCCGCCGGATCCCTTC
ACCGATGCCAGCCTGATCAAGGCCATGATGAACATCGCCCGCTACGTCGACGACGCCACGGTCAAGCGTACCTTGCGGGA
TACCGACGGACTGGGGACCGAGGCCACGCGCGCGGGAATCATGGAAACCCTCGTCACGCGCGGTTATATCGTGCGTCAGC
AAGGCGCGTTACGTGCCACTCGCACCGGACGCGCGCTCATCCAGGCCTTGCCGGATACCGCCACCCGTCCCGAGCGAACG
GCACTCTGGGAACAACGTCTTGCCGACATCGCCGAGGGCCGCGGCGATGCGGATGCCTTCCTCGACGCGCTGGTGGGTGA
TGTCCGCCAACTGCTCGACCAGGCGGATGCCGCACGCATGCGCCAGGCGCTGGCCACCACCGGCGGTGCCGAGCCGAGCT
CGCCCTCGCGGGGCAAGAAGCGCGGCACCCGGCGCGGCAGCGGCGACGCCTCGTCCAGCCCCCGACGCCGCGCCGGGAAG
TCCACGGCCAGGCGTCGCACCTCCTCCAAGAAAGGCCAGACATGA

Upstream 100 bases:

>100_bases
GCGCGCCGACTCTTCGGACGCCGTTGAGACCTCGCACCCCGTTTCGCCACGTTCCGGGGGACGTGGCAAACTGACCGCCC
GATAGCCGGAAATCGTCTCC

Downstream 100 bases:

>100_bases
TGCCCGCCTCGTCTCCTTGGTGGCTGCTCGGTGCCGGCGCCATGGGACGCCTGTTCGCCGCCCGCCTGAGCGTCGCGCGT
CCGGTGTGCCTGATCGGCCG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 654; Mature: 654

Protein sequence:

>654_residues
MRLIIAEKPSLARAIADALPTAPQRHEGYLVCGETCISWCVGHLLEQAPPDAYDARYQQWRLDHLPIVPRQWKLIPRSKA
RAQLAVIRKLLKRADTVVHAGDPDREGQLLVQQVIAHLGWRGSISRLLVSDLNRPAVRQALQCIEDNARYQSLFEAAEAR
SRADWVYGINLSRAWTLTGRQAGYQGVLSVGRVQTPVLGLIVRRDLDIEAFTPRPFYPLWADLKVEHGTLRAWWQPQAPH
PLDEQGRLLERAPAEALAAHLPGAEGHLSELQSKRQTQSAPLPYSLSALQVDAARRYKLPAKRVLDICQTLYERHQLITY
PRSDCRYLPEGHFAAAKKTLDTACAQDATLRGWYQGADFSRRSRAWNDKQVGAHHALAPTGRTFDASRLTGDEANVFRLI
ARNVLAQFYSPLVTRDVKAAFSIHGEHFRAQGREILEPGWKPLFTTRDEAPPLPALHEGEAARVVDCAVEDRQTRPPDPF
TDASLIKAMMNIARYVDDATVKRTLRDTDGLGTEATRAGIMETLVTRGYIVRQQGALRATRTGRALIQALPDTATRPERT
ALWEQRLADIAEGRGDADAFLDALVGDVRQLLDQADAARMRQALATTGGAEPSSPSRGKKRGTRRGSGDASSSPRRRAGK
STARRRTSSKKGQT

Sequences:

>Translated_654_residues
MRLIIAEKPSLARAIADALPTAPQRHEGYLVCGETCISWCVGHLLEQAPPDAYDARYQQWRLDHLPIVPRQWKLIPRSKA
RAQLAVIRKLLKRADTVVHAGDPDREGQLLVQQVIAHLGWRGSISRLLVSDLNRPAVRQALQCIEDNARYQSLFEAAEAR
SRADWVYGINLSRAWTLTGRQAGYQGVLSVGRVQTPVLGLIVRRDLDIEAFTPRPFYPLWADLKVEHGTLRAWWQPQAPH
PLDEQGRLLERAPAEALAAHLPGAEGHLSELQSKRQTQSAPLPYSLSALQVDAARRYKLPAKRVLDICQTLYERHQLITY
PRSDCRYLPEGHFAAAKKTLDTACAQDATLRGWYQGADFSRRSRAWNDKQVGAHHALAPTGRTFDASRLTGDEANVFRLI
ARNVLAQFYSPLVTRDVKAAFSIHGEHFRAQGREILEPGWKPLFTTRDEAPPLPALHEGEAARVVDCAVEDRQTRPPDPF
TDASLIKAMMNIARYVDDATVKRTLRDTDGLGTEATRAGIMETLVTRGYIVRQQGALRATRTGRALIQALPDTATRPERT
ALWEQRLADIAEGRGDADAFLDALVGDVRQLLDQADAARMRQALATTGGAEPSSPSRGKKRGTRRGSGDASSSPRRRAGK
STARRRTSSKKGQT
>Mature_654_residues
MRLIIAEKPSLARAIADALPTAPQRHEGYLVCGETCISWCVGHLLEQAPPDAYDARYQQWRLDHLPIVPRQWKLIPRSKA
RAQLAVIRKLLKRADTVVHAGDPDREGQLLVQQVIAHLGWRGSISRLLVSDLNRPAVRQALQCIEDNARYQSLFEAAEAR
SRADWVYGINLSRAWTLTGRQAGYQGVLSVGRVQTPVLGLIVRRDLDIEAFTPRPFYPLWADLKVEHGTLRAWWQPQAPH
PLDEQGRLLERAPAEALAAHLPGAEGHLSELQSKRQTQSAPLPYSLSALQVDAARRYKLPAKRVLDICQTLYERHQLITY
PRSDCRYLPEGHFAAAKKTLDTACAQDATLRGWYQGADFSRRSRAWNDKQVGAHHALAPTGRTFDASRLTGDEANVFRLI
ARNVLAQFYSPLVTRDVKAAFSIHGEHFRAQGREILEPGWKPLFTTRDEAPPLPALHEGEAARVVDCAVEDRQTRPPDPF
TDASLIKAMMNIARYVDDATVKRTLRDTDGLGTEATRAGIMETLVTRGYIVRQQGALRATRTGRALIQALPDTATRPERT
ALWEQRLADIAEGRGDADAFLDALVGDVRQLLDQADAARMRQALATTGGAEPSSPSRGKKRGTRRGSGDASSSPRRRAGK
STARRRTSSKKGQT

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=625, Percent_Identity=22.24, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1788061, Length=655, Percent_Identity=46.1068702290076, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1787529, Length=592, Percent_Identity=25.5067567567568, Blast_Score=127, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=634, Percent_Identity=24.2902208201893, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=619, Percent_Identity=22.9402261712439, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323263, Length=685, Percent_Identity=23.5036496350365, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585251, Length=671, Percent_Identity=24.441132637854, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 72727; Mature: 72727

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIIAEKPSLARAIADALPTAPQRHEGYLVCGETCISWCVGHLLEQAPPDAYDARYQQW
CEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RLDHLPIVPRQWKLIPRSKARAQLAVIRKLLKRADTVVHAGDPDREGQLLVQQVIAHLGW
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHCCC
RGSISRLLVSDLNRPAVRQALQCIEDNARYQSLFEAAEARSRADWVYGINLSRAWTLTGR
CHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCC
QAGYQGVLSVGRVQTPVLGLIVRRDLDIEAFTPRPFYPLWADLKVEHGTLRAWWQPQAPH
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEECCCCEEEEECCCCCC
PLDEQGRLLERAPAEALAAHLPGAEGHLSELQSKRQTQSAPLPYSLSALQVDAARRYKLP
CCCHHCHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
AKRVLDICQTLYERHQLITYPRSDCRYLPEGHFAAAKKTLDTACAQDATLRGWYQGADFS
HHHHHHHHHHHHHHHHEEECCCHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHH
RRSRAWNDKQVGAHHALAPTGRTFDASRLTGDEANVFRLIARNVLAQFYSPLVTRDVKAA
HHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSIHGEHFRAQGREILEPGWKPLFTTRDEAPPLPALHEGEAARVVDCAVEDRQTRPPDPF
HHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
TDASLIKAMMNIARYVDDATVKRTLRDTDGLGTEATRAGIMETLVTRGYIVRQQGALRAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEECCCCHHHH
RTGRALIQALPDTATRPERTALWEQRLADIAEGRGDADAFLDALVGDVRQLLDQADAARM
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RQALATTGGAEPSSPSRGKKRGTRRGSGDASSSPRRRAGKSTARRRTSSKKGQT
HHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRLIIAEKPSLARAIADALPTAPQRHEGYLVCGETCISWCVGHLLEQAPPDAYDARYQQW
CEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RLDHLPIVPRQWKLIPRSKARAQLAVIRKLLKRADTVVHAGDPDREGQLLVQQVIAHLGW
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHCCC
RGSISRLLVSDLNRPAVRQALQCIEDNARYQSLFEAAEARSRADWVYGINLSRAWTLTGR
CHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCC
QAGYQGVLSVGRVQTPVLGLIVRRDLDIEAFTPRPFYPLWADLKVEHGTLRAWWQPQAPH
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEEECCCCEEEEECCCCCC
PLDEQGRLLERAPAEALAAHLPGAEGHLSELQSKRQTQSAPLPYSLSALQVDAARRYKLP
CCCHHCHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
AKRVLDICQTLYERHQLITYPRSDCRYLPEGHFAAAKKTLDTACAQDATLRGWYQGADFS
HHHHHHHHHHHHHHHHEEECCCHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHH
RRSRAWNDKQVGAHHALAPTGRTFDASRLTGDEANVFRLIARNVLAQFYSPLVTRDVKAA
HHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSIHGEHFRAQGREILEPGWKPLFTTRDEAPPLPALHEGEAARVVDCAVEDRQTRPPDPF
HHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
TDASLIKAMMNIARYVDDATVKRTLRDTDGLGTEATRAGIMETLVTRGYIVRQQGALRAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEECCCCHHHH
RTGRALIQALPDTATRPERTALWEQRLADIAEGRGDADAFLDALVGDVRQLLDQADAARM
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RQALATTGGAEPSSPSRGKKRGTRRGSGDASSSPRRRAGKSTARRRTSSKKGQT
HHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]