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The map label for this gene is nifM [H]

Identifier: 92113460

GI number: 92113460

Start: 1526849

End: 1527661

Strand: Direct

Name: nifM [H]

Synonym: Csal_1334

Alternate gene names: 92113460

Gene position: 1526849-1527661 (Clockwise)

Preceding gene: 92113459

Following gene: 92113463

Centisome position: 41.3

GC content: 69.5

Gene sequence:

>813_bases
ATGCAGAAGATTGCTATCGAGCAGATTCCGGGGCGCGCCAGTCTCGCCCCCGTGCGGGTCGGCGAGATCTCCATCGATGA
GGCCGCCATCGCCCGGGAAACGCAATATCACCCGGCGGAGAGCGCCGGCGATGCTCGGTACAAGGCGGCCCGTGCCCTGG
TGGTGCGCGAGCTGCTGCGTCAGCGTGCCGGGCAGTTGGGACTGGCGGTGGAAGGCGAGTCGAATATCGAGGTCTCCATT
GCCGCGCTGCTGGAGCGCGAGCTGGAAGTCCCCGAGCCCTCCGAGGCCGACTGCCGCCGCTTCCACGCCGCCCACGCCGA
GCGCTTCAGCGAACCCACCCGTCTCAGGGTGCGGCATATCCTGCTGGCGGCGGCGCCGGACGATGCCGAGGCCCGTGATA
CCGGTTACCGTCTGGGTGAGACGCTTATCACGCAATTGAGCGAATGCCCCGAGCGCTTCACCGAGTTCGCCCAGCGCCAT
TCCGCCTGCCCCTCGAAGGACAAGGGCGGCGAGCTGGGCTGGCTGGCGCCCGGCCAGACGGTGCCCGAGCTGGATCGTGC
CCTGCAGCACCTGGCGACCGGGCTCCATGCACGGCCGCTGGCCTCCCGCTACGGCTGGCACCTGGTGAGCCTCGACGCCC
GCGAGGAGGGGCGGGCGCTGCCCTACGACGCGGTCGCCGAGCGGGTCCGGCACAGCCTGCGTGAACAGGCCACCCGCCGC
GCCCTGCGCCACTACCTGCTGGCGCTGGAGGATGACATCGGTATTCAGGGGTTTCGGCTCGACGAACAGGCGGATGGTTC
CCTGATGCAATGA

Upstream 100 bases:

>100_bases
GGCGTTATCAGGTCGTCCGCAAGCGCGGCTGAAGCGGGCCTTGCCAACGGAAACGCTCCCGATCGTCGGAACGGTTGAGA
AGAACTTCAAGGATTAAGCC

Downstream 100 bases:

>100_bases
GAGCCTGTCACCTGGCGCGTGCCCGAGGCAGCTCGTCCCAGCGCGTGGTATAGCAGGCCGAACGGTAGGCGCTGTTCATC
TGCCAGGGGGCGTCGGCGGG

Product: PpiC-type peptidyl-prolyl cis-trans isomerase

Products: Isomerized Proline [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MQKIAIEQIPGRASLAPVRVGEISIDEAAIARETQYHPAESAGDARYKAARALVVRELLRQRAGQLGLAVEGESNIEVSI
AALLERELEVPEPSEADCRRFHAAHAERFSEPTRLRVRHILLAAAPDDAEARDTGYRLGETLITQLSECPERFTEFAQRH
SACPSKDKGGELGWLAPGQTVPELDRALQHLATGLHARPLASRYGWHLVSLDAREEGRALPYDAVAERVRHSLREQATRR
ALRHYLLALEDDIGIQGFRLDEQADGSLMQ

Sequences:

>Translated_270_residues
MQKIAIEQIPGRASLAPVRVGEISIDEAAIARETQYHPAESAGDARYKAARALVVRELLRQRAGQLGLAVEGESNIEVSI
AALLERELEVPEPSEADCRRFHAAHAERFSEPTRLRVRHILLAAAPDDAEARDTGYRLGETLITQLSECPERFTEFAQRH
SACPSKDKGGELGWLAPGQTVPELDRALQHLATGLHARPLASRYGWHLVSLDAREEGRALPYDAVAERVRHSLREQATRR
ALRHYLLALEDDIGIQGFRLDEQADGSLMQ
>Mature_270_residues
MQKIAIEQIPGRASLAPVRVGEISIDEAAIARETQYHPAESAGDARYKAARALVVRELLRQRAGQLGLAVEGESNIEVSI
AALLERELEVPEPSEADCRRFHAAHAERFSEPTRLRVRHILLAAAPDDAEARDTGYRLGETLITQLSECPERFTEFAQRH
SACPSKDKGGELGWLAPGQTVPELDRALQHLATGLHARPLASRYGWHLVSLDAREEGRALPYDAVAERVRHSLREQATRR
ALRHYLLALEDDIGIQGFRLDEQADGSLMQ

Specific function: Required for the activation and stabilization of the iron-component (nifH) of nitrogenase. Probable PPIase [H]

COG id: COG0760

COG function: function code O; Parvulin-like peptidyl-prolyl isomerase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PpiC domain [H]

Homologues:

None

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014282
- InterPro:   IPR000297
- InterPro:   IPR023058
- InterPro:   IPR008880 [H]

Pfam domain/function: PF00639 Rotamase [H]

EC number: 5.2.1.8 [C]

Molecular weight: Translated: 29969; Mature: 29969

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50198 PPIC_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKIAIEQIPGRASLAPVRVGEISIDEAAIARETQYHPAESAGDARYKAARALVVRELLR
CCCCCHHHCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
QRAGQLGLAVEGESNIEVSIAALLERELEVPEPSEADCRRFHAAHAERFSEPTRLRVRHI
HHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LLAAAPDDAEARDTGYRLGETLITQLSECPERFTEFAQRHSACPSKDKGGELGWLAPGQT
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCC
VPELDRALQHLATGLHARPLASRYGWHLVSLDAREEGRALPYDAVAERVRHSLREQATRR
HHHHHHHHHHHHHCCCCCHHHHHCCEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALRHYLLALEDDIGIQGFRLDEQADGSLMQ
HHHHHHHHHCCCCCCCCEECCCCCCCCCCC
>Mature Secondary Structure
MQKIAIEQIPGRASLAPVRVGEISIDEAAIARETQYHPAESAGDARYKAARALVVRELLR
CCCCCHHHCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
QRAGQLGLAVEGESNIEVSIAALLERELEVPEPSEADCRRFHAAHAERFSEPTRLRVRHI
HHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LLAAAPDDAEARDTGYRLGETLITQLSECPERFTEFAQRHSACPSKDKGGELGWLAPGQT
HEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCC
VPELDRALQHLATGLHARPLASRYGWHLVSLDAREEGRALPYDAVAERVRHSLREQATRR
HHHHHHHHHHHHHCCCCCHHHHHCCEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALRHYLLALEDDIGIQGFRLDEQADGSLMQ
HHHHHHHHHCCCCCCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proline Imidic Peptide Bonds In Oligopeptides [C]

Specific reaction: Cis-Trans Isomerization Of Proline Imidic Peptide Bonds In Oligopeptides. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885524 [H]