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The map label for this gene is yafC [C]

Identifier: 92113463

GI number: 92113463

Start: 1529586

End: 1530479

Strand: Direct

Name: yafC [C]

Synonym: Csal_1337

Alternate gene names: 92113463

Gene position: 1529586-1530479 (Clockwise)

Preceding gene: 92113460

Following gene: 92113464

Centisome position: 41.38

GC content: 68.01

Gene sequence:

>894_bases
ATGGACCGCCTGCAGGCGATGCACATCTTTCGTGCCGTGGTCGACGCGGGGAGCTTCGCCGGTGGCGCGCGCAAGCTGGA
ACTCTCCAACGCCATGGTCAGCAAGCAGATCGCCAAGCTGGAAGCCAGCCTGGGGGTGCGGCTGTTGACGCGTACCACGC
GGCGCTTGCAGCTGACCGCCGAGGGCGAGCGCTATCTCAACGGCGCCAGTCGCCTGCTCGACGAATTGCAGGAGCTGGAA
ACGGGCCTCGGCGAGCAGCGTGGCGAGGTGCGCGGCAAGCTGCGTCTCTCCGCGCCGCTGGACTTCGGGGTGCGCGCGCT
GATCCCGGCCCTGCAGGCCTTCGAGTGTCGTCATCCCGCGGTGATGCTCGACGTCGTGCTCGAGGATCGGCGCGTCGATC
CGGTGGCCGAGGATTTCGATCTGGTGGTGCGCATCGGCGAGCTGGAGGACTCCAGCCTGATCGCGCGCCCGCTGATGGAC
ATGCCGCGCCACGTGTATGCCGCGCCGTCGTACCTGGCGCGTCACGGGACCCCGACGCATCCCGCCGACCTGCGCGGGCA
TCGTTGCCTGCACTACAGCCTGGCCCGCGAAGGGCAGTACTGGCACTTCCAGGTCGAGGGCGCGCCGTATCGGGTCCGCA
TTCGCCCGGTCATGGCCTGCAACAACGGTCGCGCCCTGACCGAAGCCGCGATACGCGGCATGGGCCTGGTCTACAAACCG
GCGTTCCTGGGCGAACCGGAAGTCGAGGCCGGACGCCTGGTGCGCGTTCTCGATGGCTTCAGCGTGGCACCGATCAGCGT
GCACCTGCTGTATGCCGAGCGTGAATTCATCCCCGCACGTTTGCGGGCGCTGGTGGACACCTTGCTGGCACACTTTGCGG
AGGCTCGCGCATGA

Upstream 100 bases:

>100_bases
GACGTTTTCGAGCACGTTCGTCCAGAACAGTCGTTACGAAAAGTCGTCAAGAACAATCGTCAAGAACCGTCGTTACGAGA
CGTCGATACGAGGGAGCCGT

Downstream 100 bases:

>100_bases
CGGCGCCGATCCTGATCTTCGACTCCGGCGTCGGCGGGCTGTCGGTGGTGGGGGCCTTGCGGGCGCGCCTGCCCGGCGCG
GCGCTGGCCTATGTCTGCGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MDRLQAMHIFRAVVDAGSFAGGARKLELSNAMVSKQIAKLEASLGVRLLTRTTRRLQLTAEGERYLNGASRLLDELQELE
TGLGEQRGEVRGKLRLSAPLDFGVRALIPALQAFECRHPAVMLDVVLEDRRVDPVAEDFDLVVRIGELEDSSLIARPLMD
MPRHVYAAPSYLARHGTPTHPADLRGHRCLHYSLAREGQYWHFQVEGAPYRVRIRPVMACNNGRALTEAAIRGMGLVYKP
AFLGEPEVEAGRLVRVLDGFSVAPISVHLLYAEREFIPARLRALVDTLLAHFAEARA

Sequences:

>Translated_297_residues
MDRLQAMHIFRAVVDAGSFAGGARKLELSNAMVSKQIAKLEASLGVRLLTRTTRRLQLTAEGERYLNGASRLLDELQELE
TGLGEQRGEVRGKLRLSAPLDFGVRALIPALQAFECRHPAVMLDVVLEDRRVDPVAEDFDLVVRIGELEDSSLIARPLMD
MPRHVYAAPSYLARHGTPTHPADLRGHRCLHYSLAREGQYWHFQVEGAPYRVRIRPVMACNNGRALTEAAIRGMGLVYKP
AFLGEPEVEAGRLVRVLDGFSVAPISVHLLYAEREFIPARLRALVDTLLAHFAEARA
>Mature_297_residues
MDRLQAMHIFRAVVDAGSFAGGARKLELSNAMVSKQIAKLEASLGVRLLTRTTRRLQLTAEGERYLNGASRLLDELQELE
TGLGEQRGEVRGKLRLSAPLDFGVRALIPALQAFECRHPAVMLDVVLEDRRVDPVAEDFDLVVRIGELEDSSLIARPLMD
MPRHVYAAPSYLARHGTPTHPADLRGHRCLHYSLAREGQYWHFQVEGAPYRVRIRPVMACNNGRALTEAAIRGMGLVYKP
AFLGEPEVEAGRLVRVLDGFSVAPISVHLLYAEREFIPARLRALVDTLLAHFAEARA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=32.6530612244898, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1789639, Length=294, Percent_Identity=31.9727891156463, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=32.9896907216495, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=33.2203389830508, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787589, Length=282, Percent_Identity=29.0780141843972, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=26.7123287671233, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787128, Length=278, Percent_Identity=27.6978417266187, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=27.2413793103448, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788706, Length=199, Percent_Identity=29.6482412060301, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786448, Length=255, Percent_Identity=30.5882352941176, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI157672245, Length=156, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790262, Length=249, Percent_Identity=24.8995983935743, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33060; Mature: 33060

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLQAMHIFRAVVDAGSFAGGARKLELSNAMVSKQIAKLEASLGVRLLTRTTRRLQLTA
CCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCHHHEECCCCEEEEEE
EGERYLNGASRLLDELQELETGLGEQRGEVRGKLRLSAPLDFGVRALIPALQAFECRHPA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCC
VMLDVVLEDRRVDPVAEDFDLVVRIGELEDSSLIARPLMDMPRHVYAAPSYLARHGTPTH
EEEEEHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHCCCHHHHHCCCCCC
PADLRGHRCLHYSLAREGQYWHFQVEGAPYRVRIRPVMACNNGRALTEAAIRGMGLVYKP
CCCCCCCEEEEEHHHCCCCEEEEEECCCCEEEEEEEEEEECCCCHHHHHHHHCCCEEECC
AFLGEPEVEAGRLVRVLDGFSVAPISVHLLYAEREFIPARLRALVDTLLAHFAEARA
CCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRLQAMHIFRAVVDAGSFAGGARKLELSNAMVSKQIAKLEASLGVRLLTRTTRRLQLTA
CCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCHHHEECCCCEEEEEE
EGERYLNGASRLLDELQELETGLGEQRGEVRGKLRLSAPLDFGVRALIPALQAFECRHPA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCC
VMLDVVLEDRRVDPVAEDFDLVVRIGELEDSSLIARPLMDMPRHVYAAPSYLARHGTPTH
EEEEEHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHCCCHHHHHCCCCCC
PADLRGHRCLHYSLAREGQYWHFQVEGAPYRVRIRPVMACNNGRALTEAAIRGMGLVYKP
CCCCCCCEEEEEHHHCCCCEEEEEECCCCEEEEEEEEEEECCCCHHHHHHHHCCCEEECC
AFLGEPEVEAGRLVRVLDGFSVAPISVHLLYAEREFIPARLRALVDTLLAHFAEARA
CCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]