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The map label for this gene is amiC [H]

Identifier: 92113400

GI number: 92113400

Start: 1452884

End: 1454377

Strand: Direct

Name: amiC [H]

Synonym: Csal_1274

Alternate gene names: 92113400

Gene position: 1452884-1454377 (Clockwise)

Preceding gene: 92113399

Following gene: 92113401

Centisome position: 39.3

GC content: 64.99

Gene sequence:

>1494_bases
TTGACGCTGTACCCCGCTATGCCTCGGCGCGCGATGCGCGTCATGCGTACCTTCCGGCGCAGCATGAGCCTGGCGTTGTT
GACGCTGCTCACGCTCGCGCAGCTGACGTCCGCGCACGCCGCCGAGGTGGACAACATTCGTACCTGGGCGGCGCCGGACC
ACGTTCGGGTGGTCTTCGATCTGTCCGACGAGGCAGAGAGTTCCATCTTCATGCTCGACGATCCACGCCGTCTGGTCGTC
GATCTGGAAGATACCCGGCTGGCCTTCGACCCCGGTGAGGTCGAGCCCGGCGACAGCGCGATCGAGGCGTTGCGCACCGG
GGTCCGCAACGGTGACGATCTGCGCGTGGTGCTGGACCTCGGGCGCGAGGTCGTGCCGCATCACTTCATGCTGCCGCCCA
CCGACCAGTACGGTCATCGCCTGGTGGTGGATCTCGACTATCCCGGCGAAAGCGCCCTCGACGAACCCATCGACCCGATC
GCCGCGATGATCCGCGATCAGGAAATGAGCGCCGAGCGGGCCCTGGTCGAAGCCAAGGCCCAGGGCAAGGATCCCGACGA
GGTCCTTGCCCGTCAGGACAGTCAGGCCAAGCCGCATCCCAAGCGCGACATCATCATCGCCGTCGATCCGGGGCACGGGG
GGGAGGATCCCGGCGCCATCGGACCGGATGGCACCTACGAGAAGAATGTGGTGCTGGCCATCGCCCGCAAGCTGAACGCA
AAGCTCAACAATACGCCGGGGTTCAAGGCCTTCCTCACCCGGGACGGCGATTATTATGTCGGACTACGGCAGCGTACCTT
GCTGGCGCGAAAGCAGAAGGCCGATTTCTTCGTCTCGGTGCATGCCGATGCGGTCAAGAGCCGGGGGCCGAGCGGCAGTT
CGGTGTATGCGCTGTCGAAGCATGGGGCCACTTCCGAGAGCGCGCGCTGGCTGGCGGCGTCGGAAAACCGTGCCGACCTG
ATCGGCGGCGTCGACGGTAGCCTGGACCTTTCCGACAAGGATGAGGTGCTGCGCGGGGTGCTGCTCGACTTGACCATGAC
CGCGACGATGAACGAGTCGCTCAGTACCGGGGGGCAGGTGCTCGACCAGCTGGGGCGTGTCAACCGCCTGCATAAGTCCC
AGGTCGAACAGGCCGGTTTCGTGGTGCTCAAGTCACCGGATATCCCGTCACTGCTGGTGGAGACGGGCTTCATTTCCAAC
CCCTCGGAGGAAAGGCGGCTCTTGTCGTCGCCTTTCCAGGCGAAACTCGCGGATGCCATCGGCACCGGCATCATCGCGCA
TTTCCGTCAGAACCCACCTCCCAGCAGCTTGCTGGCGTGGCAGCGCGATCAGGGCCGTGGCAGCGGGCCGGGCGAGTATC
GCGTTCAGGCAGGCGACACGCTGTCGGAGGTCGCCTCGCGCCATGACGTGACGATGCTGGCCCTCAAGGAAGCGAACGAT
CTCAATGGCGACATTCTGCGCGTGGGCCAGGTCCTGAAGATTCCGCAGTCGTAA

Upstream 100 bases:

>100_bases
CCGGCGAGGGCCGCGAGGTCTCGCTCGAAGGTCTCACGCCCCGCGGGCAAGCGGTAATCGCCGCCCTACACGACACATGC
CGGGACAAGGAAGCCGACTC

Downstream 100 bases:

>100_bases
GCGCGCGCATGACGCGCGCCGCTTGCGCGAGGCACAATGGTGTTATGACGCATACTCGACGCATTCACATTCTCGACCCT
CGACTCGCCAACCAGATCGC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTLYPAMPRRAMRVMRTFRRSMSLALLTLLTLAQLTSAHAAEVDNIRTWAAPDHVRVVFDLSDEAESSIFMLDDPRRLVV
DLEDTRLAFDPGEVEPGDSAIEALRTGVRNGDDLRVVLDLGREVVPHHFMLPPTDQYGHRLVVDLDYPGESALDEPIDPI
AAMIRDQEMSAERALVEAKAQGKDPDEVLARQDSQAKPHPKRDIIIAVDPGHGGEDPGAIGPDGTYEKNVVLAIARKLNA
KLNNTPGFKAFLTRDGDYYVGLRQRTLLARKQKADFFVSVHADAVKSRGPSGSSVYALSKHGATSESARWLAASENRADL
IGGVDGSLDLSDKDEVLRGVLLDLTMTATMNESLSTGGQVLDQLGRVNRLHKSQVEQAGFVVLKSPDIPSLLVETGFISN
PSEERRLLSSPFQAKLADAIGTGIIAHFRQNPPPSSLLAWQRDQGRGSGPGEYRVQAGDTLSEVASRHDVTMLALKEAND
LNGDILRVGQVLKIPQS

Sequences:

>Translated_497_residues
MTLYPAMPRRAMRVMRTFRRSMSLALLTLLTLAQLTSAHAAEVDNIRTWAAPDHVRVVFDLSDEAESSIFMLDDPRRLVV
DLEDTRLAFDPGEVEPGDSAIEALRTGVRNGDDLRVVLDLGREVVPHHFMLPPTDQYGHRLVVDLDYPGESALDEPIDPI
AAMIRDQEMSAERALVEAKAQGKDPDEVLARQDSQAKPHPKRDIIIAVDPGHGGEDPGAIGPDGTYEKNVVLAIARKLNA
KLNNTPGFKAFLTRDGDYYVGLRQRTLLARKQKADFFVSVHADAVKSRGPSGSSVYALSKHGATSESARWLAASENRADL
IGGVDGSLDLSDKDEVLRGVLLDLTMTATMNESLSTGGQVLDQLGRVNRLHKSQVEQAGFVVLKSPDIPSLLVETGFISN
PSEERRLLSSPFQAKLADAIGTGIIAHFRQNPPPSSLLAWQRDQGRGSGPGEYRVQAGDTLSEVASRHDVTMLALKEAND
LNGDILRVGQVLKIPQS
>Mature_496_residues
TLYPAMPRRAMRVMRTFRRSMSLALLTLLTLAQLTSAHAAEVDNIRTWAAPDHVRVVFDLSDEAESSIFMLDDPRRLVVD
LEDTRLAFDPGEVEPGDSAIEALRTGVRNGDDLRVVLDLGREVVPHHFMLPPTDQYGHRLVVDLDYPGESALDEPIDPIA
AMIRDQEMSAERALVEAKAQGKDPDEVLARQDSQAKPHPKRDIIIAVDPGHGGEDPGAIGPDGTYEKNVVLAIARKLNAK
LNNTPGFKAFLTRDGDYYVGLRQRTLLARKQKADFFVSVHADAVKSRGPSGSSVYALSKHGATSESARWLAASENRADLI
GGVDGSLDLSDKDEVLRGVLLDLTMTATMNESLSTGGQVLDQLGRVNRLHKSQVEQAGFVVLKSPDIPSLLVETGFISNP
SEERRLLSSPFQAKLADAIGTGIIAHFRQNPPPSSLLAWQRDQGRGSGPGEYRVQAGDTLSEVASRHDVTMLALKEANDL
NGDILRVGQVLKIPQS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=406, Percent_Identity=40.3940886699507, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI1790611, Length=416, Percent_Identity=35.3365384615385, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1788776, Length=252, Percent_Identity=44.8412698412698, Blast_Score=212, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 54175; Mature: 54044

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLYPAMPRRAMRVMRTFRRSMSLALLTLLTLAQLTSAHAAEVDNIRTWAAPDHVRVVFD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEE
LSDEAESSIFMLDDPRRLVVDLEDTRLAFDPGEVEPGDSAIEALRTGVRNGDDLRVVLDL
ECCCCCCCEEEEECCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEHECC
GREVVPHHFMLPPTDQYGHRLVVDLDYPGESALDEPIDPIAAMIRDQEMSAERALVEAKA
CHHHCCCCEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QGKDPDEVLARQDSQAKPHPKRDIIIAVDPGHGGEDPGAIGPDGTYEKNVVLAIARKLNA
CCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
KLNNTPGFKAFLTRDGDYYVGLRQRTLLARKQKADFFVSVHADAVKSRGPSGSSVYALSK
CCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCEEEEEEC
HGATSESARWLAASENRADLIGGVDGSLDLSDKDEVLRGVLLDLTMTATMNESLSTGGQV
CCCCCCCCEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LDQLGRVNRLHKSQVEQAGFVVLKSPDIPSLLVETGFISNPSEERRLLSSPFQAKLADAI
HHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHH
GTGIIAHFRQNPPPSSLLAWQRDQGRGSGPGEYRVQAGDTLSEVASRHDVTMLALKEAND
HHHHHHHHCCCCCCHHHEEEECCCCCCCCCCCEEEECCCHHHHHHHHCCEEEEEEECCCC
LNGDILRVGQVLKIPQS
CCCHHEEHHHHHCCCCC
>Mature Secondary Structure 
TLYPAMPRRAMRVMRTFRRSMSLALLTLLTLAQLTSAHAAEVDNIRTWAAPDHVRVVFD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEE
LSDEAESSIFMLDDPRRLVVDLEDTRLAFDPGEVEPGDSAIEALRTGVRNGDDLRVVLDL
ECCCCCCCEEEEECCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEHECC
GREVVPHHFMLPPTDQYGHRLVVDLDYPGESALDEPIDPIAAMIRDQEMSAERALVEAKA
CHHHCCCCEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QGKDPDEVLARQDSQAKPHPKRDIIIAVDPGHGGEDPGAIGPDGTYEKNVVLAIARKLNA
CCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
KLNNTPGFKAFLTRDGDYYVGLRQRTLLARKQKADFFVSVHADAVKSRGPSGSSVYALSK
CCCCCCCCEEEEECCCCEEEEHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCEEEEEEC
HGATSESARWLAASENRADLIGGVDGSLDLSDKDEVLRGVLLDLTMTATMNESLSTGGQV
CCCCCCCCEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LDQLGRVNRLHKSQVEQAGFVVLKSPDIPSLLVETGFISNPSEERRLLSSPFQAKLADAI
HHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHH
GTGIIAHFRQNPPPSSLLAWQRDQGRGSGPGEYRVQAGDTLSEVASRHDVTMLALKEAND
HHHHHHHHCCCCCCHHHEEEECCCCCCCCCCCEEEECCCHHHHHHHHCCEEEEEEECCCC
LNGDILRVGQVLKIPQS
CCCHHEEHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]