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The map label for this gene is trpA

Identifier: 92113388

GI number: 92113388

Start: 1440677

End: 1441492

Strand: Direct

Name: trpA

Synonym: Csal_1262

Alternate gene names: 92113388

Gene position: 1440677-1441492 (Clockwise)

Preceding gene: 92113387

Following gene: 92113389

Centisome position: 38.97

GC content: 66.67

Gene sequence:

>816_bases
ATGAATCGTATCGATAAACGCTTCGCGGCGCTCAAGTCTCAACAGCGCCGCGCGTTGATTCCTTATATCACCGCGGGCGA
TCCGGCGCCGCAGCATACCGTGGGGTTCATGCACGCCTGCGTCGAGGCTGGCGCCGACGTGCTGGAGCTGGGCGTGCCGT
TTTCCGACCCCATGGCCGATGGCCCGGTGATCCAGAAAGCCTGCGAGCGTGCGCTCGCCAAGGGCACGCGGTTGACCGAT
GTTCTCGACATGGTCGCGACGTTTCGCGCCACCGATGCGGAAACGCCCGTGGTCCTGATGGGGTATCTCAACCCCGTCGA
GCGCATGGGATACGAGCATTTCGCGCGGCGCGCGCGGGAGGCGGGCGTCGACGGCGTGCTGATGGTCGACATGCCGCCGG
AGGAGATGGCTGAGGTCGCACCGCTGTTCTCCGAGCACGGGCTGCAGTCGATCTTTCTGCTTGCCCCTACCACCTCGCCG
TCGCGGGCCGCTACAATATGTCGACATGGCGAAGGCTATCTGTACTACGTATCGCTCAAGGGCGTTACCGGTGCCGCCTC
GCTGGACGTCGATGCCGTCGAGCGCCAGTTGGCGCCGCTTCGCGAGATGACCGACCTGCCGCTATGCGTCGGGTTCGGGA
TTCGGGATGGCGACACGGCGGCGGCAGTGGGCCGGGTGGCCGACGGGGTCATCGTCGGCAGTGCTCTGGTGTCGCGCATC
GCCGAAAACGCCGAGACCCCCGAGGCCATACCGGCGGCGCTCAAGGCGGTGCTCGGCGACATGCGCGATGCGCTGGACCG
TTCCGACGGCCGCTGA

Upstream 100 bases:

>100_bases
AGCACATCATCGTCAATCTCTCCGGGCGTGGCGACAAGGACATCCACACGGTGGCCAAGATCGACGGTCTGGAATTCTAA
GCGCTGGCGGAGACATGACT

Downstream 100 bases:

>100_bases
TGCCCTACGGCATTCGCCAACGACTATTCGCTGCATGGGGCCACGGATGTGGCTTCATGCGTCAGACAAGCAACACGGAA
GCGTCTCAGAACCATGAGCT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNRIDKRFAALKSQQRRALIPYITAGDPAPQHTVGFMHACVEAGADVLELGVPFSDPMADGPVIQKACERALAKGTRLTD
VLDMVATFRATDAETPVVLMGYLNPVERMGYEHFARRAREAGVDGVLMVDMPPEEMAEVAPLFSEHGLQSIFLLAPTTSP
SRAATICRHGEGYLYYVSLKGVTGAASLDVDAVERQLAPLREMTDLPLCVGFGIRDGDTAAAVGRVADGVIVGSALVSRI
AENAETPEAIPAALKAVLGDMRDALDRSDGR

Sequences:

>Translated_271_residues
MNRIDKRFAALKSQQRRALIPYITAGDPAPQHTVGFMHACVEAGADVLELGVPFSDPMADGPVIQKACERALAKGTRLTD
VLDMVATFRATDAETPVVLMGYLNPVERMGYEHFARRAREAGVDGVLMVDMPPEEMAEVAPLFSEHGLQSIFLLAPTTSP
SRAATICRHGEGYLYYVSLKGVTGAASLDVDAVERQLAPLREMTDLPLCVGFGIRDGDTAAAVGRVADGVIVGSALVSRI
AENAETPEAIPAALKAVLGDMRDALDRSDGR
>Mature_271_residues
MNRIDKRFAALKSQQRRALIPYITAGDPAPQHTVGFMHACVEAGADVLELGVPFSDPMADGPVIQKACERALAKGTRLTD
VLDMVATFRATDAETPVVLMGYLNPVERMGYEHFARRAREAGVDGVLMVDMPPEEMAEVAPLFSEHGLQSIFLLAPTTSP
SRAATICRHGEGYLYYVSLKGVTGAASLDVDAVERQLAPLREMTDLPLCVGFGIRDGDTAAAVGRVADGVIVGSALVSRI
AENAETPEAIPAALKAVLGDMRDALDRSDGR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=265, Percent_Identity=35.8490566037736, Blast_Score=157, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6321412, Length=237, Percent_Identity=38.3966244725738, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_CHRSD (Q1QY41)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_573316.1
- ProteinModelPortal:   Q1QY41
- SMR:   Q1QY41
- STRING:   Q1QY41
- GeneID:   4027583
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_1262
- NMPDR:   fig|290398.4.peg.1252
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- BioCyc:   CSAL290398:CSAL_1262-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28920; Mature: 28920

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIDKRFAALKSQQRRALIPYITAGDPAPQHTVGFMHACVEAGADVLELGVPFSDPMAD
CCHHHHHHHHHHHHCCCEECCEEECCCCCCHHHHHHHHHHHHCCCHHEECCCCCCCCCCC
GPVIQKACERALAKGTRLTDVLDMVATFRATDAETPVVLMGYLNPVERMGYEHFARRARE
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHCHHHHHHHHHH
AGVDGVLMVDMPPEEMAEVAPLFSEHGLQSIFLLAPTTSPSRAATICRHGEGYLYYVSLK
CCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEEEEC
GVTGAASLDVDAVERQLAPLREMTDLPLCVGFGIRDGDTAAAVGRVADGVIVGSALVSRI
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AENAETPEAIPAALKAVLGDMRDALDRSDGR
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNRIDKRFAALKSQQRRALIPYITAGDPAPQHTVGFMHACVEAGADVLELGVPFSDPMAD
CCHHHHHHHHHHHHCCCEECCEEECCCCCCHHHHHHHHHHHHCCCHHEECCCCCCCCCCC
GPVIQKACERALAKGTRLTDVLDMVATFRATDAETPVVLMGYLNPVERMGYEHFARRARE
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHCHHHHHHHHHH
AGVDGVLMVDMPPEEMAEVAPLFSEHGLQSIFLLAPTTSPSRAATICRHGEGYLYYVSLK
CCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEEEEC
GVTGAASLDVDAVERQLAPLREMTDLPLCVGFGIRDGDTAAAVGRVADGVIVGSALVSRI
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AENAETPEAIPAALKAVLGDMRDALDRSDGR
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA