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The map label for this gene is gltX

Identifier: 92113337

GI number: 92113337

Start: 1384812

End: 1386296

Strand: Direct

Name: gltX

Synonym: Csal_1211

Alternate gene names: 92113337

Gene position: 1384812-1386296 (Clockwise)

Preceding gene: 92113336

Following gene: 92113339

Centisome position: 37.46

GC content: 61.62

Gene sequence:

>1485_bases
ATGACCGTACGTACGCGTATCGCACCATCGCCCACCGGCGATCCTCATGTCGGCACGGCCTATATCGCCTTGTTCAATCT
GTGCTTCGCGCGCCAGCATGGTGGACAGTTCATTCTGCGCATCGAGGACACGGACCGGGTGCGCTCGACGGCCGAGTCGG
AGCAGATGATTCTCGACTCGCTGCGCTGGCTGGGCCTGGAATGGGACGAGGGGCCTGACGTGGGCGGCCCGCATGGCCCC
TATCGTCAAAGTGAGCGGGGCGATATCTACGCGCAATATGCGCAGCAGCTGCTCGATGCCGGGCACGCCTTCAAGTGCTA
CCGGACCAGCGAGGAGCTCGACGAACTGCGCGAAGCGCGCAAGGCGGCGGGTCTGCACCTGGCGCTCAAGCCGGCGGATC
TGGCGCTTCCCGACGATGAAGTGGCGCGTCGCGAGCGTGAGCAGTGGCCTTATGTGGTGCGCATGAAGGTGCCCGGGGAG
GGCAGCTGCGTGGTCAGCGACATGCTGCGAGGCGAGATCGAGGTGGACTGGGCCCAGGTCGATGCGCAGATCCTGCTCAA
GTCCGATGGCATGCCGACCTACCACCTGGCCAATGTCGTCGACGATCACCTGATGGGCATCACCCACGTGATGCGTGGCG
AGGAATGGATCAACTCGGCACCCAAGCACAAGCTTCTGTACGAGTATTTCGACTGGCCGATGCCGACGCTATGCCACATG
CCGCTGCTGCGTAACCCGGACAAGTCGAAGCTCTCCAAGCGCAAGAATCCGACTTCCATCAATTATTACCGGCGGATGGG
GTTTCTGCCGCAGGCAGTGACCAATTATCTGGGCCGCATGGGATGGTCAATGCCCGACGACCGCGAGAAGTTCACGCTCG
AGGAGATGATGGCGAACTTCGATATCCAGCGTGTTTCCCTGGGGGGGCCGGTCTTCGATCTCGAAAAGCTGACCTGGCTC
AATGGGGTGTACATTCGCGAGGATCTGGACGATGCAGCGCTGCTCCAGGCGTTGCGCGAGTGGGCGTTCAATGATGCCTA
CGTCAATCAGATTCTGCCGCAGGTGCGCCCGCGCATCTCGACACTGTCCGATGTCATGCCGCTGGCCGGGCATTTCTTTT
CCGGTGTGCCGCCGCTCGAGGCATCCAGCTTCGACGGCATCAAGCTGGAGCATGAAACGCTGCTCAAGTTGCTGCAGTTC
CTGACCTGGCGTCTCGAAGGCGTCTCCCGCTGGGAGAAGGACGTGCTGCTCGATGACGTCAAGGCTCTGGCCGAGGCCTT
CGACATGAAGATGAAGGCGTTCCTGGCGCCGGTTTTCATCGCTGTGACCGGCAGTGCGACATCGACATCGGTGTTGGATG
CCATGGCCATTCTGGGGTCGGACATGACCCGCGCGCGGCTGCGTCATGCCATCGAGGTGCTGGGTGGTGTCTCCAAGAAA
CAGGCCAAGCGCTTCGAGAAGGAATATCGGCAGTTGCAGGTGTGA

Upstream 100 bases:

>100_bases
CGAGCTTCGGTCTCGCCGGCGTTTGGCCCGGTGGTGCTGATGCCGGATAGGCGCATGCGTATAATGGTGCCATCGTCAAT
CACCAATGATCGGGAATTCC

Downstream 100 bases:

>100_bases
GTCGAGGCGCCTTGCGCCTGGAAGAGGGGGGAACGCCGCTATGCCACCTGGAGGGGCAGGCACGCTCTCCCTTCTTCCTG
AAAAGTGTTGACGTGGAAGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGQFILRIEDTDRVRSTAESEQMILDSLRWLGLEWDEGPDVGGPHGP
YRQSERGDIYAQYAQQLLDAGHAFKCYRTSEELDELREARKAAGLHLALKPADLALPDDEVARREREQWPYVVRMKVPGE
GSCVVSDMLRGEIEVDWAQVDAQILLKSDGMPTYHLANVVDDHLMGITHVMRGEEWINSAPKHKLLYEYFDWPMPTLCHM
PLLRNPDKSKLSKRKNPTSINYYRRMGFLPQAVTNYLGRMGWSMPDDREKFTLEEMMANFDIQRVSLGGPVFDLEKLTWL
NGVYIREDLDDAALLQALREWAFNDAYVNQILPQVRPRISTLSDVMPLAGHFFSGVPPLEASSFDGIKLEHETLLKLLQF
LTWRLEGVSRWEKDVLLDDVKALAEAFDMKMKAFLAPVFIAVTGSATSTSVLDAMAILGSDMTRARLRHAIEVLGGVSKK
QAKRFEKEYRQLQV

Sequences:

>Translated_494_residues
MTVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGQFILRIEDTDRVRSTAESEQMILDSLRWLGLEWDEGPDVGGPHGP
YRQSERGDIYAQYAQQLLDAGHAFKCYRTSEELDELREARKAAGLHLALKPADLALPDDEVARREREQWPYVVRMKVPGE
GSCVVSDMLRGEIEVDWAQVDAQILLKSDGMPTYHLANVVDDHLMGITHVMRGEEWINSAPKHKLLYEYFDWPMPTLCHM
PLLRNPDKSKLSKRKNPTSINYYRRMGFLPQAVTNYLGRMGWSMPDDREKFTLEEMMANFDIQRVSLGGPVFDLEKLTWL
NGVYIREDLDDAALLQALREWAFNDAYVNQILPQVRPRISTLSDVMPLAGHFFSGVPPLEASSFDGIKLEHETLLKLLQF
LTWRLEGVSRWEKDVLLDDVKALAEAFDMKMKAFLAPVFIAVTGSATSTSVLDAMAILGSDMTRARLRHAIEVLGGVSKK
QAKRFEKEYRQLQV
>Mature_493_residues
TVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGQFILRIEDTDRVRSTAESEQMILDSLRWLGLEWDEGPDVGGPHGPY
RQSERGDIYAQYAQQLLDAGHAFKCYRTSEELDELREARKAAGLHLALKPADLALPDDEVARREREQWPYVVRMKVPGEG
SCVVSDMLRGEIEVDWAQVDAQILLKSDGMPTYHLANVVDDHLMGITHVMRGEEWINSAPKHKLLYEYFDWPMPTLCHMP
LLRNPDKSKLSKRKNPTSINYYRRMGFLPQAVTNYLGRMGWSMPDDREKFTLEEMMANFDIQRVSLGGPVFDLEKLTWLN
GVYIREDLDDAALLQALREWAFNDAYVNQILPQVRPRISTLSDVMPLAGHFFSGVPPLEASSFDGIKLEHETLLKLLQFL
TWRLEGVSRWEKDVLLDDVKALAEAFDMKMKAFLAPVFIAVTGSATSTSVLDAMAILGSDMTRARLRHAIEVLGGVSKKQ
AKRFEKEYRQLQV

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=492, Percent_Identity=31.7073170731707, Blast_Score=239, Evalue=3e-63,
Organism=Escherichia coli, GI1788743, Length=493, Percent_Identity=34.6855983772819, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI226510925, Length=257, Percent_Identity=32.295719844358, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71993394, Length=357, Percent_Identity=37.8151260504202, Blast_Score=230, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6324540, Length=357, Percent_Identity=39.4957983193277, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24665321, Length=499, Percent_Identity=33.4669338677355, Blast_Score=247, Evalue=2e-65,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CHRSD (Q1QY92)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_573265.1
- ProteinModelPortal:   Q1QY92
- SMR:   Q1QY92
- STRING:   Q1QY92
- GeneID:   4027356
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_1211
- NMPDR:   fig|290398.4.peg.1036
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   CSAL290398:CSAL_1211-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56183; Mature: 56052

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGQFILRIEDTDRVRSTAESEQMILDS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
LRWLGLEWDEGPDVGGPHGPYRQSERGDIYAQYAQQLLDAGHAFKCYRTSEELDELREAR
HHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
KAAGLHLALKPADLALPDDEVARREREQWPYVVRMKVPGEGSCVVSDMLRGEIEVDWAQV
HHCCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCEEEHHHH
DAQILLKSDGMPTYHLANVVDDHLMGITHVMRGEEWINSAPKHKLLYEYFDWPMPTLCHM
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCCCHHHHCC
PLLRNPDKSKLSKRKNPTSINYYRRMGFLPQAVTNYLGRMGWSMPDDREKFTLEEMMANF
HHHCCCCHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
DIQRVSLGGPVFDLEKLTWLNGVYIREDLDDAALLQALREWAFNDAYVNQILPQVRPRIS
CCEEEECCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TLSDVMPLAGHFFSGVPPLEASSFDGIKLEHETLLKLLQFLTWRLEGVSRWEKDVLLDDV
HHHHHHHHHHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KALAEAFDMKMKAFLAPVFIAVTGSATSTSVLDAMAILGSDMTRARLRHAIEVLGGVSKK
HHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHH
QAKRFEKEYRQLQV
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGQFILRIEDTDRVRSTAESEQMILDS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
LRWLGLEWDEGPDVGGPHGPYRQSERGDIYAQYAQQLLDAGHAFKCYRTSEELDELREAR
HHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
KAAGLHLALKPADLALPDDEVARREREQWPYVVRMKVPGEGSCVVSDMLRGEIEVDWAQV
HHCCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCEEEHHHH
DAQILLKSDGMPTYHLANVVDDHLMGITHVMRGEEWINSAPKHKLLYEYFDWPMPTLCHM
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCCCHHHHCC
PLLRNPDKSKLSKRKNPTSINYYRRMGFLPQAVTNYLGRMGWSMPDDREKFTLEEMMANF
HHHCCCCHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
DIQRVSLGGPVFDLEKLTWLNGVYIREDLDDAALLQALREWAFNDAYVNQILPQVRPRIS
CCEEEECCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TLSDVMPLAGHFFSGVPPLEASSFDGIKLEHETLLKLLQFLTWRLEGVSRWEKDVLLDDV
HHHHHHHHHHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KALAEAFDMKMKAFLAPVFIAVTGSATSTSVLDAMAILGSDMTRARLRHAIEVLGGVSKK
HHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHH
QAKRFEKEYRQLQV
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA