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The map label for this gene is nuoF2 [H]

Identifier: 92113124

GI number: 92113124

Start: 1119576

End: 1121363

Strand: Direct

Name: nuoF2 [H]

Synonym: Csal_0996

Alternate gene names: 92113124

Gene position: 1119576-1121363 (Clockwise)

Preceding gene: 92113123

Following gene: 92113125

Centisome position: 30.29

GC content: 67.23

Gene sequence:

>1788_bases
ATGGCGGTCCGCCACGTCGGCAGCGCTTTACCGGGCCAGTCAGTGCCTACCGATAACAACGAGCACGCCATGACCGTCGA
GACTCTCACTCCCAAGCGTCGCTTTCGCAACAAGCTCCGCGGACGCGACCTGCCCCCCGTCGCCCTGGCGGCGCTTCGCC
AACTCCTCGGCGACGAACGCGAGGACGGTGAACGGCGACGCCGCGATCTCCTGATCGAGCATCTGCACACCATCCAGGAT
GCCCAGGGCCACCTCTCGCTGGTGATGCTTCGCGCGCTTGCCACCTACATGAATCTGCCCATGGCCGCGGTCTACGAGAC
GGCGACCTTTTACGCTCACTTCGATGTGGTCCATGACGATCAGTCGCCACCGCCCGAGGTGACGGTCCGGGTCTGCGATT
CGCTGTCCTGCCGGCTGGCCGGTGCCGAGGCGCTCAAGGCAAAGCTGGCGGCCGGCGTCGATCCGGAAGCGGTCCGGGTG
GTGCGAGCCCCTTGCATGGGGCGCTGCGACACCGCGCCGGTGGTGGAAGTGGGGCATCATCACGTGCGCTTCGCCACCCA
TGAAGGGGTGGCCTCGGTGATCGATACCGGTCATTACCATCCCGAGGCGATTCTCTGGCAGCGGCTGGCGGATTATCGCG
CCGAGGGCGGCTATGCATTGCTCGCCGACTGCCACGCGGGACGCGTCACGGTCGAGGCGTTGATGCAGGCGCTGGAAACC
GCCAATCTGCGCGGCCTGGGCGGCGCCGGCTTCCCCACCTTCAAGAAATGGACCTTCGTGCGCCAGGAAGCGGGGCCGCG
CTACTGTGCCATCAACGCCGACGAAGGCGAACCCGGCACCTTCAAGGACCGCGTCTATCTGGAACGCTCGCCGCACCGCT
TCCTCGAGGGCGCCCTGGTCAGTGCCTGGGCGGTGGAAGCCGAGGCGCTCTACATTTACCTGCGCGACGAGTATCCGGGC
CTGCACCGCGTATTGCGCGAGGCGATCGCCGAGCTCGAGGACGAGGGCCTGGTGGTGCCGGACTATATCGTGCTGCGGCG
CGGTGCCGGTGCCTATATCTGCGGCGAGGAGTCGGCGATGATCGAGTCGCTGGAGGGCAAGCCCGGCAAGCCTCGCCATC
GGCCGCCCTTCGTCGCCCAGAGAGGGTTGTTCGATCGTCCCACGCTGGTCAACAACGTGGAAACCGTCTACTGGATCCCG
CTGATCTGGCAGCGCGGTGCCGAGGCCTTCGCGCGCCAGGGCCGCCACGGTCGGGTGGGGCTGCGCAGCTTCTCGGTCTC
CGGCCGGGTCAAGCACCCCGGCGTGCACTTGGCTCCGGCGGGCATCACCCTCGAGGAGTTGATCGAGGAATATTGCGGCG
GCATGGCCGAGGGGCATCGCCTTGTCGCCTACCTGCCCGGCGGCGCCTCGGGCGGCATCCTGCCGGCGTCCAAGGCGAAC
GTGCCGCTGGACTTCGACACCCTGCAGGCGTACGGCTGCTTCATCGGCTCGGCGGCGGTGATCGTGCTGTCCGACCAGGA
CGATCTGCGTGCCGTCGCCACCAATCTGCTGGCCTTCTTCGCCGACGAGTCCTGCGGTCAGTGCACCCCTTGTCGGGTGG
GCACCGAGAAGATGCTGACTCTGCTCGAGGGCGACGAATGGGATGCCGCGACCCTGACGCGCCTCTCGCAGGTGATGATG
GATGCCTCCATCTGCGGCCTCGGGCAGGCAGCGCCCAATCCGGTGCTGGGGCTGCTCAAGGACTTCCGCGGTGAACTCGC
CGCCCAGAACGTGATCGTCAAGGGATAA

Upstream 100 bases:

>100_bases
CTGACACCATGGTCTACCCGACATCGCCCCGCCCGGGTGCCAATATGCCATCAGGCGCCCGCTTCAGGGCGGGGCAGCCG
GTACCCGGAAGGCGTCTTCC

Downstream 100 bases:

>100_bases
GGGAGACATGGCATGAGCAAGCAATGCTCGAACACAAGCTTCACCCTGACCCTGGACGGGGTGGCAGTAAGCGCCACTGC
CGGGGAGACGCTCTGGCAGG

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MAVRHVGSALPGQSVPTDNNEHAMTVETLTPKRRFRNKLRGRDLPPVALAALRQLLGDEREDGERRRRDLLIEHLHTIQD
AQGHLSLVMLRALATYMNLPMAAVYETATFYAHFDVVHDDQSPPPEVTVRVCDSLSCRLAGAEALKAKLAAGVDPEAVRV
VRAPCMGRCDTAPVVEVGHHHVRFATHEGVASVIDTGHYHPEAILWQRLADYRAEGGYALLADCHAGRVTVEALMQALET
ANLRGLGGAGFPTFKKWTFVRQEAGPRYCAINADEGEPGTFKDRVYLERSPHRFLEGALVSAWAVEAEALYIYLRDEYPG
LHRVLREAIAELEDEGLVVPDYIVLRRGAGAYICGEESAMIESLEGKPGKPRHRPPFVAQRGLFDRPTLVNNVETVYWIP
LIWQRGAEAFARQGRHGRVGLRSFSVSGRVKHPGVHLAPAGITLEELIEEYCGGMAEGHRLVAYLPGGASGGILPASKAN
VPLDFDTLQAYGCFIGSAAVIVLSDQDDLRAVATNLLAFFADESCGQCTPCRVGTEKMLTLLEGDEWDAATLTRLSQVMM
DASICGLGQAAPNPVLGLLKDFRGELAAQNVIVKG

Sequences:

>Translated_595_residues
MAVRHVGSALPGQSVPTDNNEHAMTVETLTPKRRFRNKLRGRDLPPVALAALRQLLGDEREDGERRRRDLLIEHLHTIQD
AQGHLSLVMLRALATYMNLPMAAVYETATFYAHFDVVHDDQSPPPEVTVRVCDSLSCRLAGAEALKAKLAAGVDPEAVRV
VRAPCMGRCDTAPVVEVGHHHVRFATHEGVASVIDTGHYHPEAILWQRLADYRAEGGYALLADCHAGRVTVEALMQALET
ANLRGLGGAGFPTFKKWTFVRQEAGPRYCAINADEGEPGTFKDRVYLERSPHRFLEGALVSAWAVEAEALYIYLRDEYPG
LHRVLREAIAELEDEGLVVPDYIVLRRGAGAYICGEESAMIESLEGKPGKPRHRPPFVAQRGLFDRPTLVNNVETVYWIP
LIWQRGAEAFARQGRHGRVGLRSFSVSGRVKHPGVHLAPAGITLEELIEEYCGGMAEGHRLVAYLPGGASGGILPASKAN
VPLDFDTLQAYGCFIGSAAVIVLSDQDDLRAVATNLLAFFADESCGQCTPCRVGTEKMLTLLEGDEWDAATLTRLSQVMM
DASICGLGQAAPNPVLGLLKDFRGELAAQNVIVKG
>Mature_594_residues
AVRHVGSALPGQSVPTDNNEHAMTVETLTPKRRFRNKLRGRDLPPVALAALRQLLGDEREDGERRRRDLLIEHLHTIQDA
QGHLSLVMLRALATYMNLPMAAVYETATFYAHFDVVHDDQSPPPEVTVRVCDSLSCRLAGAEALKAKLAAGVDPEAVRVV
RAPCMGRCDTAPVVEVGHHHVRFATHEGVASVIDTGHYHPEAILWQRLADYRAEGGYALLADCHAGRVTVEALMQALETA
NLRGLGGAGFPTFKKWTFVRQEAGPRYCAINADEGEPGTFKDRVYLERSPHRFLEGALVSAWAVEAEALYIYLRDEYPGL
HRVLREAIAELEDEGLVVPDYIVLRRGAGAYICGEESAMIESLEGKPGKPRHRPPFVAQRGLFDRPTLVNNVETVYWIPL
IWQRGAEAFARQGRHGRVGLRSFSVSGRVKHPGVHLAPAGITLEELIEEYCGGMAEGHRLVAYLPGGASGGILPASKANV
PLDFDTLQAYGCFIGSAAVIVLSDQDDLRAVATNLLAFFADESCGQCTPCRVGTEKMLTLLEGDEWDAATLTRLSQVMMD
ASICGLGQAAPNPVLGLLKDFRGELAAQNVIVKG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=371, Percent_Identity=43.3962264150943, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI20149568, Length=371, Percent_Identity=43.3962264150943, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1788620, Length=424, Percent_Identity=35.8490566037736, Blast_Score=252, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI17531727, Length=398, Percent_Identity=41.9597989949749, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI19920794, Length=371, Percent_Identity=42.3180592991914, Blast_Score=275, Evalue=6e-74,
Organism=Drosophila melanogaster, GI45550452, Length=368, Percent_Identity=37.7717391304348, Blast_Score=239, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24653819, Length=316, Percent_Identity=37.3417721518987, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24653817, Length=294, Percent_Identity=36.734693877551, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 64923; Mature: 64792

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRHVGSALPGQSVPTDNNEHAMTVETLTPKRRFRNKLRGRDLPPVALAALRQLLGDER
CCCCHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
EDGERRRRDLLIEHLHTIQDAQGHLSLVMLRALATYMNLPMAAVYETATFYAHFDVVHDD
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHEEEEEEEEEECC
QSPPPEVTVRVCDSLSCRLAGAEALKAKLAAGVDPEAVRVVRAPCMGRCDTAPVVEVGHH
CCCCHHHHHHHHCCCCEEECCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHCCCC
HVRFATHEGVASVIDTGHYHPEAILWQRLADYRAEGGYALLADCHAGRVTVEALMQALET
EEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
ANLRGLGGAGFPTFKKWTFVRQEAGPRYCAINADEGEPGTFKDRVYLERSPHRFLEGALV
CCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHH
SAWAVEAEALYIYLRDEYPGLHRVLREAIAELEDEGLVVPDYIVLRRGAGAYICGEESAM
HHHEEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEECCCHHEEECCCCEEEECCHHHH
IESLEGKPGKPRHRPPFVAQRGLFDRPTLVNNVETVYWIPLIWQRGAEAFARQGRHGRVG
HHHHCCCCCCCCCCCCCHHHCCCCCCCCHHCCCCEEEEEHHHHHCCHHHHHHCCCCCCCE
LRSFSVSGRVKHPGVHLAPAGITLEELIEEYCGGMAEGHRLVAYLPGGASGGILPASKAN
EEEEECCCCEECCCEEEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCC
VPLDFDTLQAYGCFIGSAAVIVLSDQDDLRAVATNLLAFFADESCGQCTPCRVGTEKMLT
CCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LLEGDEWDAATLTRLSQVMMDASICGLGQAAPNPVLGLLKDFRGELAAQNVIVKG
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEECC
>Mature Secondary Structure 
AVRHVGSALPGQSVPTDNNEHAMTVETLTPKRRFRNKLRGRDLPPVALAALRQLLGDER
CCCHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
EDGERRRRDLLIEHLHTIQDAQGHLSLVMLRALATYMNLPMAAVYETATFYAHFDVVHDD
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHEEEEEEEEEECC
QSPPPEVTVRVCDSLSCRLAGAEALKAKLAAGVDPEAVRVVRAPCMGRCDTAPVVEVGHH
CCCCHHHHHHHHCCCCEEECCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHCCCC
HVRFATHEGVASVIDTGHYHPEAILWQRLADYRAEGGYALLADCHAGRVTVEALMQALET
EEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHH
ANLRGLGGAGFPTFKKWTFVRQEAGPRYCAINADEGEPGTFKDRVYLERSPHRFLEGALV
CCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHH
SAWAVEAEALYIYLRDEYPGLHRVLREAIAELEDEGLVVPDYIVLRRGAGAYICGEESAM
HHHEEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEECCCHHEEECCCCEEEECCHHHH
IESLEGKPGKPRHRPPFVAQRGLFDRPTLVNNVETVYWIPLIWQRGAEAFARQGRHGRVG
HHHHCCCCCCCCCCCCCHHHCCCCCCCCHHCCCCEEEEEHHHHHCCHHHHHHCCCCCCCE
LRSFSVSGRVKHPGVHLAPAGITLEELIEEYCGGMAEGHRLVAYLPGGASGGILPASKAN
EEEEECCCCEECCCEEEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCC
VPLDFDTLQAYGCFIGSAAVIVLSDQDDLRAVATNLLAFFADESCGQCTPCRVGTEKMLT
CCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LLEGDEWDAATLTRLSQVMMDASICGLGQAAPNPVLGLLKDFRGELAAQNVIVKG
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]