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The map label for this gene is rluB [H]

Identifier: 92112979

GI number: 92112979

Start: 950903

End: 951763

Strand: Direct

Name: rluB [H]

Synonym: Csal_0851

Alternate gene names: 92112979

Gene position: 950903-951763 (Clockwise)

Preceding gene: 92112978

Following gene: 92112980

Centisome position: 25.72

GC content: 65.27

Gene sequence:

>861_bases
ATGAACACGCCTACCGAGAAATTACAGAAGGTCCTGGCCCGCGCGGGCTTCGGCTCGCGCCGCGAGATGGAGACCGCCAT
CGCCGATGGCCGCGTCAAGGTCAACGGTCAGGTCGCCACCCTGGGCGATCGCATCGAGACACGCGACCGCGTCACCTTCG
ACGAGCGGCCGGTCAATCTGCGCGATGCCGAGGAAGTGCCCCGGCGCGTCATCATGTACAACAAGCCCGAAGGCGAGCTG
TGCACGCGACGTGACCCCGAGGGTCGGCGTACCGTGTTCGAGCGCCTGCCGCGTCTCAAGGGCGAGCGCTGGATCGCCGT
CGGTCGTCTCGACATCAACACCAGCGGCCTGCTGCTGTTCACCACCGATGGCGAGCTCGCCAACCGCCTGATGCATCCCG
CCACGCAGGTCGAGCGCGAATACGCGGTGCGCGTGATGGGCAACGTTCGCCAGGAACACGTCAATGCCATGGTCGAGGGC
GTGATGCTCGAGGATGGCCCGGCGCGTTTCACCGATGTGCAGGAATTCGGCGGCGAGGGCATCAACACCTGGTTCCACGT
GGTGATCCTTGAGGGACGCAACCGCGAGGTACGGCGTCTCTGGGAATCTCAAGGACTCACGGTGAGTCGTCTCAAGCGTG
TGCGCTACGGCAACATCTTTCTCGACAAGCGCGCCAAGGCCGGCGAATGGGTCGAGCTGTCGCAGGATGAGATCGACGAC
CTGGCGCAGCTGGCGGACCTGCCAGCGCGCAAGGTGCCCGAACTGACGCCCGACGAGCAGAATCGCTGGAGCCGTGACAA
GAACAAGCGCCGTCCGGTCAATGCCATGCGCAAGCCGAAGTCGTCGCGGCGCTCGCGGTGA

Upstream 100 bases:

>100_bases
GCGCCCGCCGGCGCACCGAGCAGGCCGAATCGTCCCGAGCGGGCGACGACGCCTCCGCGGCGGACGCGGAACATTCATCA
GACGAGACAACGTTCGATCC

Downstream 100 bases:

>100_bases
GCGCGTTCCCGGCAAAAAAATCTTGTCAATGTCGAGCGTTATCAGTACTATGTGCGCCCTGTCGGGACGGGTCGTTAGCT
CAGTGGTAGAGCAGTTGGCT

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNTPTEKLQKVLARAGFGSRREMETAIADGRVKVNGQVATLGDRIETRDRVTFDERPVNLRDAEEVPRRVIMYNKPEGEL
CTRRDPEGRRTVFERLPRLKGERWIAVGRLDINTSGLLLFTTDGELANRLMHPATQVEREYAVRVMGNVRQEHVNAMVEG
VMLEDGPARFTDVQEFGGEGINTWFHVVILEGRNREVRRLWESQGLTVSRLKRVRYGNIFLDKRAKAGEWVELSQDEIDD
LAQLADLPARKVPELTPDEQNRWSRDKNKRRPVNAMRKPKSSRRSR

Sequences:

>Translated_286_residues
MNTPTEKLQKVLARAGFGSRREMETAIADGRVKVNGQVATLGDRIETRDRVTFDERPVNLRDAEEVPRRVIMYNKPEGEL
CTRRDPEGRRTVFERLPRLKGERWIAVGRLDINTSGLLLFTTDGELANRLMHPATQVEREYAVRVMGNVRQEHVNAMVEG
VMLEDGPARFTDVQEFGGEGINTWFHVVILEGRNREVRRLWESQGLTVSRLKRVRYGNIFLDKRAKAGEWVELSQDEIDD
LAQLADLPARKVPELTPDEQNRWSRDKNKRRPVNAMRKPKSSRRSR
>Mature_286_residues
MNTPTEKLQKVLARAGFGSRREMETAIADGRVKVNGQVATLGDRIETRDRVTFDERPVNLRDAEEVPRRVIMYNKPEGEL
CTRRDPEGRRTVFERLPRLKGERWIAVGRLDINTSGLLLFTTDGELANRLMHPATQVEREYAVRVMGNVRQEHVNAMVEG
VMLEDGPARFTDVQEFGGEGINTWFHVVILEGRNREVRRLWESQGLTVSRLKRVRYGNIFLDKRAKAGEWVELSQDEIDD
LAQLADLPARKVPELTPDEQNRWSRDKNKRRPVNAMRKPKSSRRSR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=283, Percent_Identity=54.416961130742, Blast_Score=294, Evalue=4e-81,
Organism=Escherichia coli, GI1790453, Length=247, Percent_Identity=31.1740890688259, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87081838, Length=194, Percent_Identity=34.020618556701, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=29.8319327731092, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 33026; Mature: 33026

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPTEKLQKVLARAGFGSRREMETAIADGRVKVNGQVATLGDRIETRDRVTFDERPVNL
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCEEEECCCHHHHCCCCEECCCCCCC
RDAEEVPRRVIMYNKPEGELCTRRDPEGRRTVFERLPRLKGERWIAVGRLDINTSGLLLF
CCHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEE
TTDGELANRLMHPATQVEREYAVRVMGNVRQEHVNAMVEGVMLEDGPARFTDVQEFGGEG
ECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCC
INTWFHVVILEGRNREVRRLWESQGLTVSRLKRVRYGNIFLDKRAKAGEWVELSQDEIDD
CCCEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCEECCHHHHHH
LAQLADLPARKVPELTPDEQNRWSRDKNKRRPVNAMRKPKSSRRSR
HHHHHCCCHHHCCCCCCCCHHHHHCCHHCCCCHHHHHCCCHHCCCC
>Mature Secondary Structure
MNTPTEKLQKVLARAGFGSRREMETAIADGRVKVNGQVATLGDRIETRDRVTFDERPVNL
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCEEEECCCHHHHCCCCEECCCCCCC
RDAEEVPRRVIMYNKPEGELCTRRDPEGRRTVFERLPRLKGERWIAVGRLDINTSGLLLF
CCHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEE
TTDGELANRLMHPATQVEREYAVRVMGNVRQEHVNAMVEGVMLEDGPARFTDVQEFGGEG
ECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCC
INTWFHVVILEGRNREVRRLWESQGLTVSRLKRVRYGNIFLDKRAKAGEWVELSQDEIDD
CCCEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCEECCHHHHHH
LAQLADLPARKVPELTPDEQNRWSRDKNKRRPVNAMRKPKSSRRSR
HHHHHCCCHHHCCCCCCCCHHHHHCCHHCCCCHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]