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The map label for this gene is puuB [H]

Identifier: 92112906

GI number: 92112906

Start: 870672

End: 871961

Strand: Direct

Name: puuB [H]

Synonym: Csal_0778

Alternate gene names: 92112906

Gene position: 870672-871961 (Clockwise)

Preceding gene: 92112905

Following gene: 92112907

Centisome position: 23.55

GC content: 64.5

Gene sequence:

>1290_bases
ATGACCCAGCAACGCTGTGCTTCCTATTACACCGCATCGATCGTCCAGGAATCCGACTATCCGCGACTGGAAGGTGAGCA
TCGTGTCGACGTGGCGATCGTCGGCGGCGGCTTCACCGGCGTGGCCACGGCGGTCGAGCTGGCCGAGCGCGGCTATCGCG
TGGCCATCGTCGAGGCCAACCGGATCGGCTGGGGGGCCAGCGGACGCAACGGCGGGCAGGTGACCGGCAGTCTCTCCGGC
CAGGACGCCATGGCCCGAGAAATGCGCCGCACGCTGGGCGACGACGCCGAAAGCTTCGTCTGGAACCTGCGCTGGCGCGG
GCATCGGATCATCAAGCAGCGGGTCGAAAAGTATGCGATCCCCTGCGACCTCAAGCACGGCCACCTGCAGGCGGCCTACA
AGCCCAGCCACATCAAGGCGTTGCGACAGGATTTCGAGGCGTCGCAGCACCATGCCATCGGCGAGCATGTGCACTGGCTG
GATGCCAAGGCGGTGCGCGAGGTGGTCGGTACCGAGCTCTATCACGGGGCGATCCGCAACGATTACAACATGCACCTGCA
CCCGCTCAACCTGTGCCTCGGGGAGGCCCGCGCGGCAGCAAGCCTGGGGACGCGGATCTTCGAGAATTCGCCGGTGGTGG
ATATCGAGCACGGCGATACGCCGGTGGTGGTCGGCGAGCACGGCCGGGTGACAGCCGACAGCGTGATCCTGGCCGGCAAC
GCCTATCACCGCCTCGAGCGCAAGCAGCTGAAGGGCAAGTTGTTTCCGGCATCGCTGGGCATCGTCACGACGGCGCCGCT
ATCGCCCGAACAGGTGCAGTCCATCGACCCCGAGGATCTTGCGGTCTATGACACGCGTTTCGTACTCGACTACTACCGAC
TGACCGCCGACAAGCGGCTTCTCTTCGGTGGCGGCGCCAACTACTCGGGGCGCGATTCACAAGACATCGCCTCGGAGTTG
CGGCCGCGCCTGGAGCACACCTTCCCGCAGCTCGAGGGCATTGCCATCGATTTTCAATGGCAGGGCATGGCAGGCATCGT
CATCAACCGCATTCCCCAGCTCGGCAAGCTCTCGGACAACGTCTACTACGCCCAGGGCTACTCTGGCCACGGCATCGCCA
CCTCGCACATCGTCGGCGAGATCATGGCCAATGCGGTTTCCGGCACGCTAGAGGAGTTCGATGCCTTCGCCAACGTGCGG
CACATCCGCATCCCGCTCGACGACCGGCTCGGCAATGCACTGCTCGCGGCCGGCATGTGGTACTACCAACTGCTCGAAAA
ACTCAAATAG

Upstream 100 bases:

>100_bases
GGGGTAAATGAGTATTTTGACCGGGCTGGCGTTCCAGTCGATTTTTAACGCCGTATGGGCGAGCGCAGGCATTTTTTCAG
ACAAAGCGTAAGGAGCAGGC

Downstream 100 bases:

>100_bases
CAATTACTCGAAAACTGAAATAGCGGCGCTTTGGCGCCCGACAATTCGGCTTCCCGTCATTCACGAAGGCACAACAACAA
CTCCGCTGAGGTGAGACACG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTQQRCASYYTASIVQESDYPRLEGEHRVDVAIVGGGFTGVATAVELAERGYRVAIVEANRIGWGASGRNGGQVTGSLSG
QDAMAREMRRTLGDDAESFVWNLRWRGHRIIKQRVEKYAIPCDLKHGHLQAAYKPSHIKALRQDFEASQHHAIGEHVHWL
DAKAVREVVGTELYHGAIRNDYNMHLHPLNLCLGEARAAASLGTRIFENSPVVDIEHGDTPVVVGEHGRVTADSVILAGN
AYHRLERKQLKGKLFPASLGIVTTAPLSPEQVQSIDPEDLAVYDTRFVLDYYRLTADKRLLFGGGANYSGRDSQDIASEL
RPRLEHTFPQLEGIAIDFQWQGMAGIVINRIPQLGKLSDNVYYAQGYSGHGIATSHIVGEIMANAVSGTLEEFDAFANVR
HIRIPLDDRLGNALLAAGMWYYQLLEKLK

Sequences:

>Translated_429_residues
MTQQRCASYYTASIVQESDYPRLEGEHRVDVAIVGGGFTGVATAVELAERGYRVAIVEANRIGWGASGRNGGQVTGSLSG
QDAMAREMRRTLGDDAESFVWNLRWRGHRIIKQRVEKYAIPCDLKHGHLQAAYKPSHIKALRQDFEASQHHAIGEHVHWL
DAKAVREVVGTELYHGAIRNDYNMHLHPLNLCLGEARAAASLGTRIFENSPVVDIEHGDTPVVVGEHGRVTADSVILAGN
AYHRLERKQLKGKLFPASLGIVTTAPLSPEQVQSIDPEDLAVYDTRFVLDYYRLTADKRLLFGGGANYSGRDSQDIASEL
RPRLEHTFPQLEGIAIDFQWQGMAGIVINRIPQLGKLSDNVYYAQGYSGHGIATSHIVGEIMANAVSGTLEEFDAFANVR
HIRIPLDDRLGNALLAAGMWYYQLLEKLK
>Mature_428_residues
TQQRCASYYTASIVQESDYPRLEGEHRVDVAIVGGGFTGVATAVELAERGYRVAIVEANRIGWGASGRNGGQVTGSLSGQ
DAMAREMRRTLGDDAESFVWNLRWRGHRIIKQRVEKYAIPCDLKHGHLQAAYKPSHIKALRQDFEASQHHAIGEHVHWLD
AKAVREVVGTELYHGAIRNDYNMHLHPLNLCLGEARAAASLGTRIFENSPVVDIEHGDTPVVVGEHGRVTADSVILAGNA
YHRLERKQLKGKLFPASLGIVTTAPLSPEQVQSIDPEDLAVYDTRFVLDYYRLTADKRLLFGGGANYSGRDSQDIASELR
PRLEHTFPQLEGIAIDFQWQGMAGIVINRIPQLGKLSDNVYYAQGYSGHGIATSHIVGEIMANAVSGTLEEFDAFANVRH
IRIPLDDRLGNALLAAGMWYYQLLEKLK

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=427, Percent_Identity=39.1100702576112, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47487; Mature: 47356

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQRCASYYTASIVQESDYPRLEGEHRVDVAIVGGGFTGVATAVELAERGYRVAIVEAN
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEC
RIGWGASGRNGGQVTGSLSGQDAMAREMRRTLGDDAESFVWNLRWRGHRIIKQRVEKYAI
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHEEEEEECHHHHHHHHHHHHCC
PCDLKHGHLQAAYKPSHIKALRQDFEASQHHAIGEHVHWLDAKAVREVVGTELYHGAIRN
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DYNMHLHPLNLCLGEARAAASLGTRIFENSPVVDIEHGDTPVVVGEHGRVTADSVILAGN
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEECCEEEECC
AYHRLERKQLKGKLFPASLGIVTTAPLSPEQVQSIDPEDLAVYDTRFVLDYYRLTADKRL
HHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCEE
LFGGGANYSGRDSQDIASELRPRLEHTFPQLEGIAIDFQWQGMAGIVINRIPQLGKLSDN
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCC
VYYAQGYSGHGIATSHIVGEIMANAVSGTLEEFDAFANVRHIRIPLDDRLGNALLAAGMW
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHCHHHHHHHHH
YYQLLEKLK
HHHHHHHCC
>Mature Secondary Structure 
TQQRCASYYTASIVQESDYPRLEGEHRVDVAIVGGGFTGVATAVELAERGYRVAIVEAN
CHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEEC
RIGWGASGRNGGQVTGSLSGQDAMAREMRRTLGDDAESFVWNLRWRGHRIIKQRVEKYAI
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHEEEEEECHHHHHHHHHHHHCC
PCDLKHGHLQAAYKPSHIKALRQDFEASQHHAIGEHVHWLDAKAVREVVGTELYHGAIRN
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DYNMHLHPLNLCLGEARAAASLGTRIFENSPVVDIEHGDTPVVVGEHGRVTADSVILAGN
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEECCEEEECC
AYHRLERKQLKGKLFPASLGIVTTAPLSPEQVQSIDPEDLAVYDTRFVLDYYRLTADKRL
HHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCEE
LFGGGANYSGRDSQDIASELRPRLEHTFPQLEGIAIDFQWQGMAGIVINRIPQLGKLSDN
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCC
VYYAQGYSGHGIATSHIVGEIMANAVSGTLEEFDAFANVRHIRIPLDDRLGNALLAAGMW
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHCHHHHHHHHH
YYQLLEKLK
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]