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The map label for this gene is puuA [H]

Identifier: 92112905

GI number: 92112905

Start: 869132

End: 870502

Strand: Direct

Name: puuA [H]

Synonym: Csal_0777

Alternate gene names: 92112905

Gene position: 869132-870502 (Clockwise)

Preceding gene: 92112902

Following gene: 92112906

Centisome position: 23.51

GC content: 67.32

Gene sequence:

>1371_bases
ATGGCGCAGATATCGCAGGCTCCGTTTGCCCGCAGCACCGACCGCCCTCACGAGGACGCGGCCACCGACCGGGTCGAAGT
GCTGTGCATCGACATGAACGGCATTCCGCGCGGCAAGTGGGTGCCCGCCGAACAGCTCGACAAGGTGATGGAGGGGCAGG
TGCGCCTGCCGCTCTCGACGCAGTCGCTGGATATCTGGGGCGAGGACAACGATGCGCTGACCGGGCTGTCGCAGTCGATC
GGCGACCCCGACGGGGTCTGTCACGCCGATGAGCGTACCTTGACGCCACTGCCCTGGGCCAAGGGCCACCAGGTGCTCAC
CACCCTGCACGATTTGAACGGCACGCCGAGTTTCATGGACCCGCGCGCCATGCTCGCCGCGGTCGTCGAGCGCTTTCGTC
GCCACGGCTGGACGCCGGTGGTCGCCATCGAGCTGGAGTTCTACCTGCTCGATGCCGAGGCTCGGCACGCGCTCGAGCCG
AGGCCGCCCGCGCCGCTGTGTCCGGGTGGGGATCCCCGGGGGCAGCAGCTCTACGAGCTCGAGACGATGGATGCGGTGGC
GCCGGTCCTCGACACCATCCGCGCGTTCGCCCGCGCCCAGAACATCCCGGCGGATACGCTGATCGCCGAGTTCGGGCCGG
GGCAGTTCGAGGTCAATCTCTGGCATCGCCCGGATGCCCTGGCCGCCGCCGACGATGCGCTGTACTTCAAGCGTCTCGTG
GCCCAGGCCGCCCGTCATCACGGCCTGGCGAGCACCTTCATGGCCAAGCCCTACACCGATCAGGCGGGATCGGGCATGCA
TCTGCACGTCAGTGTGCTCGATGAAGACGGCAACAATATCTTCGATCGCGACAACGGCGCCGGACATCTCGACGCTGCCA
TCGGCGGCGTGATGCACAGCTTGCTCGATGGTCAGGCGATCTTCGCGCCGCACGGCAATTCCTACCGCCGCTTTCAACCC
GACAGCTTCGCGCCGATCGAGTGCAACTGGGGGCGCGATCATCGCGGTGCGGCCGTGCGCGTGCCGGAATGGCAAGGCGC
CGCGGCGCGTCTGGAACATCGCGTGGCCGGCGCCGATGCCAACCCTTATCTGGTCGCCACGGCGCTGCTGGGGGGCATTC
TGCATGGGCTTGAGCAACGCATCGCGCCGCCACCGGCCATCGAGGACGAAGCCCACGCGCGGCAGCGCTCCGAGCATCTC
ACCCATGATTGGCTCACCGCGCTCGAGCGTTTCGCCGCCTCGCCGGCCATGGCCGATATCTTCGGCACGGCGTATCGAGA
TCTCTACGCCAGGATCAAGCGTCACGAGGCCCGGGAGCTCATGGCCAGGGTCACCGATGTCGACCGCCAGACGTATCTTT
CCCGTCTTTAA

Upstream 100 bases:

>100_bases
GCAATCACGATACCGGCTTGCTTGTGGGGTTGATGGGGCTCTGCTAGTGTGATCACACATATGTGATCACACCGGACCAT
AACAAGATCGAGAGTCCCCT

Downstream 100 bases:

>100_bases
GCTCTGTCTGAAAAATCGACGAGCGATGGCCAGACAAGGCAAAAATTGGCGAAAAAGCGGAGTTTACGCGGGGTAAATGA
GTATTTTGACCGGGCTGGCG

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAQISQAPFARSTDRPHEDAATDRVEVLCIDMNGIPRGKWVPAEQLDKVMEGQVRLPLSTQSLDIWGEDNDALTGLSQSI
GDPDGVCHADERTLTPLPWAKGHQVLTTLHDLNGTPSFMDPRAMLAAVVERFRRHGWTPVVAIELEFYLLDAEARHALEP
RPPAPLCPGGDPRGQQLYELETMDAVAPVLDTIRAFARAQNIPADTLIAEFGPGQFEVNLWHRPDALAAADDALYFKRLV
AQAARHHGLASTFMAKPYTDQAGSGMHLHVSVLDEDGNNIFDRDNGAGHLDAAIGGVMHSLLDGQAIFAPHGNSYRRFQP
DSFAPIECNWGRDHRGAAVRVPEWQGAAARLEHRVAGADANPYLVATALLGGILHGLEQRIAPPPAIEDEAHARQRSEHL
THDWLTALERFAASPAMADIFGTAYRDLYARIKRHEARELMARVTDVDRQTYLSRL

Sequences:

>Translated_456_residues
MAQISQAPFARSTDRPHEDAATDRVEVLCIDMNGIPRGKWVPAEQLDKVMEGQVRLPLSTQSLDIWGEDNDALTGLSQSI
GDPDGVCHADERTLTPLPWAKGHQVLTTLHDLNGTPSFMDPRAMLAAVVERFRRHGWTPVVAIELEFYLLDAEARHALEP
RPPAPLCPGGDPRGQQLYELETMDAVAPVLDTIRAFARAQNIPADTLIAEFGPGQFEVNLWHRPDALAAADDALYFKRLV
AQAARHHGLASTFMAKPYTDQAGSGMHLHVSVLDEDGNNIFDRDNGAGHLDAAIGGVMHSLLDGQAIFAPHGNSYRRFQP
DSFAPIECNWGRDHRGAAVRVPEWQGAAARLEHRVAGADANPYLVATALLGGILHGLEQRIAPPPAIEDEAHARQRSEHL
THDWLTALERFAASPAMADIFGTAYRDLYARIKRHEARELMARVTDVDRQTYLSRL
>Mature_455_residues
AQISQAPFARSTDRPHEDAATDRVEVLCIDMNGIPRGKWVPAEQLDKVMEGQVRLPLSTQSLDIWGEDNDALTGLSQSIG
DPDGVCHADERTLTPLPWAKGHQVLTTLHDLNGTPSFMDPRAMLAAVVERFRRHGWTPVVAIELEFYLLDAEARHALEPR
PPAPLCPGGDPRGQQLYELETMDAVAPVLDTIRAFARAQNIPADTLIAEFGPGQFEVNLWHRPDALAAADDALYFKRLVA
QAARHHGLASTFMAKPYTDQAGSGMHLHVSVLDEDGNNIFDRDNGAGHLDAAIGGVMHSLLDGQAIFAPHGNSYRRFQPD
SFAPIECNWGRDHRGAAVRVPEWQGAAARLEHRVAGADANPYLVATALLGGILHGLEQRIAPPPAIEDEAHARQRSEHLT
HDWLTALERFAASPAMADIFGTAYRDLYARIKRHEARELMARVTDVDRQTYLSRL

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081870, Length=376, Percent_Identity=41.4893617021277, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1790301, Length=163, Percent_Identity=36.8098159509202, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50240; Mature: 50109

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQISQAPFARSTDRPHEDAATDRVEVLCIDMNGIPRGKWVPAEQLDKVMEGQVRLPLST
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEECCCC
QSLDIWGEDNDALTGLSQSIGDPDGVCHADERTLTPLPWAKGHQVLTTLHDLNGTPSFMD
CCEEEECCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
PRAMLAAVVERFRRHGWTPVVAIELEFYLLDAEARHALEPRPPAPLCPGGDPRGQQLYEL
HHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCEEEEH
ETMDAVAPVLDTIRAFARAQNIPADTLIAEFGPGQFEVNLWHRPDALAAADDALYFKRLV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
AQAARHHGLASTFMAKPYTDQAGSGMHLHVSVLDEDGNNIFDRDNGAGHLDAAIGGVMHS
HHHHHHCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LLDGQAIFAPHGNSYRRFQPDSFAPIECNWGRDHRGAAVRVPEWQGAAARLEHRVAGADA
HHCCCEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCEEECCCCCCHHHHHHHHHCCCCC
NPYLVATALLGGILHGLEQRIAPPPAIEDEAHARQRSEHLTHDWLTALERFAASPAMADI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FGTAYRDLYARIKRHEARELMARVTDVDRQTYLSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQISQAPFARSTDRPHEDAATDRVEVLCIDMNGIPRGKWVPAEQLDKVMEGQVRLPLST
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEECCCC
QSLDIWGEDNDALTGLSQSIGDPDGVCHADERTLTPLPWAKGHQVLTTLHDLNGTPSFMD
CCEEEECCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
PRAMLAAVVERFRRHGWTPVVAIELEFYLLDAEARHALEPRPPAPLCPGGDPRGQQLYEL
HHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCEEEEH
ETMDAVAPVLDTIRAFARAQNIPADTLIAEFGPGQFEVNLWHRPDALAAADDALYFKRLV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
AQAARHHGLASTFMAKPYTDQAGSGMHLHVSVLDEDGNNIFDRDNGAGHLDAAIGGVMHS
HHHHHHCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
LLDGQAIFAPHGNSYRRFQPDSFAPIECNWGRDHRGAAVRVPEWQGAAARLEHRVAGADA
HHCCCEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCEEECCCCCCHHHHHHHHHCCCCC
NPYLVATALLGGILHGLEQRIAPPPAIEDEAHARQRSEHLTHDWLTALERFAASPAMADI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FGTAYRDLYARIKRHEARELMARVTDVDRQTYLSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]