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The map label for this gene is lutR [H]

Identifier: 92112882

GI number: 92112882

Start: 847423

End: 848193

Strand: Direct

Name: lutR [H]

Synonym: Csal_0753

Alternate gene names: 92112882

Gene position: 847423-848193 (Clockwise)

Preceding gene: 92112881

Following gene: 92112883

Centisome position: 22.92

GC content: 62.65

Gene sequence:

>771_bases
ATGGCTGAGGAGACGACCCGAGGGAAGGGCGAGGAACTGCGCGGCGCCGAGAAGGTGTTCGGGTATTTCCGCGATCAGGT
CCTTTCGGGGGCGCTGAGCCCCGGGGATCGTCTGCCCAGCGAGCGCGAGCTGTCGCTGACCCTGGGAGTGGGGCGGCCGT
TGCTGCGCGAGGTCATGCGGTCACTCAACATGCTGGGCGTGCTCGATATCCGCCATGGCAAGGGGGCTTTCATCGCCCGC
GCCAACTTCGGAATGCTGTCGGATTTCTTCACCTTTTATCTGGCCCAGGAGCAGAGCGCGCTCGATGACGTCATGCAGGC
CCGTATCGCCATCGAGTGTCAGGCTATCCGCCTCGCCTGCGAGAGAGCCACGGATGCCGACATTTCCCGCATCGAGACCT
GCTTCACGCAGTTGACGGAGACCGTGGACGACCCGGAGCTGGGCGGTCGCTCGGATTATGAGTTCCACATCGCCATCGTG
GAGGCGAGCCACAGCCAGACGTTGATCACCCTCTACCACGCCATCTCCAACATGCTGCTGAAAAGTCACGTCGAGCGGCG
AAGAACGACGGCGAACGCACCCGAGATCATCGAGAGCCACATCGACTCCCACCGCCAGGTGTTCTTGGCAATCGTCGAGG
GCGATGGCGACCTGGCCCACCAGAGGCTCCGCGAACACTTCGCGATCGGCGACGAACTGCGCCGGCGAAGCATCATTGCT
GCCCATACCAAGGGCAGCGCCAGCACGTACTCAACCGACAAATATAAGTGA

Upstream 100 bases:

>100_bases
GCTTCCCCGCCAATGTCGAGCTTCGAAGCTTCGCTGATGCTTCAGTTAGAACAACGATGGCGTGTCATTGGTGCCAAGAG
GTAAGACCAGAGGAGGATGC

Downstream 100 bases:

>100_bases
GGCACGCATGTTCACGAGAATCGGCATGGGAGTGGCCACGGCGGCATTGGCGCTGGGCCTGACCACTACGGCATCCGCTG
AAACCCTGCGCTACGCCCAC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAEETTRGKGEELRGAEKVFGYFRDQVLSGALSPGDRLPSERELSLTLGVGRPLLREVMRSLNMLGVLDIRHGKGAFIAR
ANFGMLSDFFTFYLAQEQSALDDVMQARIAIECQAIRLACERATDADISRIETCFTQLTETVDDPELGGRSDYEFHIAIV
EASHSQTLITLYHAISNMLLKSHVERRRTTANAPEIIESHIDSHRQVFLAIVEGDGDLAHQRLREHFAIGDELRRRSIIA
AHTKGSASTYSTDKYK

Sequences:

>Translated_256_residues
MAEETTRGKGEELRGAEKVFGYFRDQVLSGALSPGDRLPSERELSLTLGVGRPLLREVMRSLNMLGVLDIRHGKGAFIAR
ANFGMLSDFFTFYLAQEQSALDDVMQARIAIECQAIRLACERATDADISRIETCFTQLTETVDDPELGGRSDYEFHIAIV
EASHSQTLITLYHAISNMLLKSHVERRRTTANAPEIIESHIDSHRQVFLAIVEGDGDLAHQRLREHFAIGDELRRRSIIA
AHTKGSASTYSTDKYK
>Mature_255_residues
AEETTRGKGEELRGAEKVFGYFRDQVLSGALSPGDRLPSERELSLTLGVGRPLLREVMRSLNMLGVLDIRHGKGAFIARA
NFGMLSDFFTFYLAQEQSALDDVMQARIAIECQAIRLACERATDADISRIETCFTQLTETVDDPELGGRSDYEFHIAIVE
ASHSQTLITLYHAISNMLLKSHVERRRTTANAPEIIESHIDSHRQVFLAIVEGDGDLAHQRLREHFAIGDELRRRSIIAA
HTKGSASTYSTDKYK

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=204, Percent_Identity=32.3529411764706, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994955, Length=218, Percent_Identity=29.8165137614679, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786303, Length=210, Percent_Identity=29.5238095238095, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790780, Length=229, Percent_Identity=25.3275109170306, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789353, Length=205, Percent_Identity=25.3658536585366, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789621, Length=205, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 28653; Mature: 28522

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEETTRGKGEELRGAEKVFGYFRDQVLSGALSPGDRLPSERELSLTLGVGRPLLREVMR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCEEEEECCCCHHHHHHHH
SLNMLGVLDIRHGKGAFIARANFGMLSDFFTFYLAQEQSALDDVMQARIAIECQAIRLAC
HCCHHEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERATDADISRIETCFTQLTETVDDPELGGRSDYEFHIAIVEASHSQTLITLYHAISNMLL
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
KSHVERRRTTANAPEIIESHIDSHRQVFLAIVEGDGDLAHQRLREHFAIGDELRRRSIIA
HHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCCHHHHHHCEEE
AHTKGSASTYSTDKYK
EECCCCCCCCCCCCCC
>Mature Secondary Structure 
AEETTRGKGEELRGAEKVFGYFRDQVLSGALSPGDRLPSERELSLTLGVGRPLLREVMR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCEEEEECCCCHHHHHHHH
SLNMLGVLDIRHGKGAFIARANFGMLSDFFTFYLAQEQSALDDVMQARIAIECQAIRLAC
HCCHHEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERATDADISRIETCFTQLTETVDDPELGGRSDYEFHIAIVEASHSQTLITLYHAISNMLL
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
KSHVERRRTTANAPEIIESHIDSHRQVFLAIVEGDGDLAHQRLREHFAIGDELRRRSIIA
HHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHCCCHHHHHHCEEE
AHTKGSASTYSTDKYK
EECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]